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| amino acid analysis by lc-ms/ms: Amino Acid Analysis Michail A. Alterman, Peter Hunziker, 2011-12-02 Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences. |
| amino acid analysis by lc-ms/ms: Techniques in Protein Chemistry III Ruth Hogue Angeletti, 2014-06-28 Techniques in Protein Chemistry III compiles papers presented at the Fifth Protein Society Symposium in Baltimore on June 22-26, 1991. This book discusses the protein and peptide recovery from PVDF membranes; high-sensitivity peptide mapping utilizing reversed-phase microbore and microcolumn liquid chromatography; and capillary electrophoresis for preparation of peptides and direct determination of amino acids. The TFMSA/TFA cleavage in t-Boc peptide synthesis; applications of automatic PTC amino acid analysis; and identification of O-glycosylation sites with a gas phase sequencer are also elaborated. This text likewise covers the conformational stability of the molten globule of cytochrome c and role of aqueous solvation in protein folding. This publication is useful to students and researchers interested in methods and research approaches on protein chemistry. |
| amino acid analysis by lc-ms/ms: Branched Chain Amino Acids in Clinical Nutrition Rajkumar Rajendram, Victor R. Preedy, Vinood B. Patel, 2014-11-14 This is the first volume in a 2-volume compendium that is the go-to source for both research- and practice-oriented information on the importance of branched chain amino acids in maintaining the nutritional status and overall health of individuals, especially those with certain disease conditions. Over 150 well recognized and respected contributors have come together to compile these up-to-date and well-referenced works. The volumes will serve the reader as the benchmarks in this complex area of interrelationships between dietary protein intakes and individual amino acid supplementation, the unique role of the branched chain amino acids in the synthesis of brain neurotransmitters, collagen formation, insulin and glucose modulation and the functioning of all organ systems that are involved in the maintenance of the body’s metabolic integrity. Moreover, the physiological, genetic and pathological interactions between plasma levels of branched chain amino acids and aromatic amino acids are clearly delineated so that students as well as practitioners can better understand the complexities of these interactions. Branched Chain Amino Acids in Clinical Nutrition: Volume 1 covers basic processes at the cellular level, inherited defects in branched chain amino acid metabolism, and experimental models of growth and disease states. |
| amino acid analysis by lc-ms/ms: Handbook of Solid Phase Microextraction Janusz Pawliszyn, 2011-12-01 The relatively new technique of solid phase microextraction (SPME) is an important tool to prepare samples both in the lab and on-site. SPME is a green technology because it eliminates organic solvents from analytical laboratory and can be used in environmental, food and fragrance, and forensic and drug analysis. This handbook offers a thorough background of the theory and practical implementation of SPME. SPME protocols are presented outlining each stage of the method and providing useful tips and potential pitfalls. In addition, devices and fiber coatings, automated SPME systems, SPME method development, and In Vivo applications are discussed. This handbook is essential for its discussion of the latest SPME developments as well as its in depth information on the history, theory, and practical application of the method. - Practical application of Solid Phase Microextraction methods including detailed steps - Provides history of extraction methods to better understand the process - Suitable for all levels, from beginning student to experienced practitioner |
| amino acid analysis by lc-ms/ms: Laboratory Guide to the Methods in Biochemical Genetics Nenad Blau, Marinus Duran, K. Michael Gibson, 2008-05-31 This manual deals specifically with laboratory approaches to diagnosing inborn errors of metabolism. The key feature is that each chapter is sufficiently detailed so that any individual can adopt the described method into their own respective laboratory. |
| amino acid analysis by lc-ms/ms: Protein and Peptide Analysis by LC-MS Thomas Letzel, 2011-07-22 This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more. |
| amino acid analysis by lc-ms/ms: Advances in Cyanobacterial Biology Prashant Kumar Singh, Ajay Kumar, Vipin Kumar Singh, Alok Kumar Shrivistava, 2020-02-19 Advances in Cyanobacterial Biology presents the novel, practical, and theoretical aspects of cyanobacteria, providing a better understanding of basic and advanced biotechnological application in the field of sustainable agriculture. Chapters have been designed to deal with the different aspects of cyanobacteria including their role in the evolution of life, cyanobacterial diversity and classification, isolation, and characterization of cyanobacteria through biochemical and molecular approaches, phylogeny and biogeography of cyanobacteria, symbiosis, Cyanobacterial photosynthesis, morphological and physiological adaptation to abiotic stresses, stress-tolerant cyanobacterium, biological nitrogen fixation. Other topics include circadian rhythms, genetics and molecular biology of abiotic stress responses, application of cyanobacteria and cyanobacterial mats in wastewater treatments, use as a source of novel stress-responsive genes for development of stress tolerance and as a source of biofuels, industrial application, as biofertilizer, cyanobacterial blooms, use in Nano-technology and nanomedicines as well as potential applications. This book will be important for academics and researchers working in cyanobacteria, cyanobacterial environmental biology, cyanobacterial agriculture and cyanobacterial molecular biologists. |
| amino acid analysis by lc-ms/ms: Neuroproteomics Oscar Alzate, 2009-10-26 In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg |
| amino acid analysis by lc-ms/ms: Targeted Biomarker Quantitation by LC-MS Naidong Weng, Wenying Jian, 2017-07-31 The first book to offer a blueprint for overcoming the challenges to successfully quantifying biomarkers in living organisms The demand among scientists and clinicians for targeted quantitation experiments has experienced explosive growth in recent years. While there are a few books dedicated to bioanalysis and biomarkers in general, until now there were none devoted exclusively to addressing critical issues surrounding this area of intense research. Target Biomarker Quantitation by LC-MS provides a detailed blueprint for quantifying biomarkers in biological systems. It uses numerous real-world cases to exemplify key concepts, all of which were carefully selected and presented so as to allow the concepts they embody to be easily expanded to future applications, including new biomarker development. Target Biomarker Quantitation by LC-MS primarily focuses on the assay establishment for biomarker quantitation—a critical issue rarely treated in depth. It offers comprehensive coverage of three core areas of biomarker assay establishment: the relationship between the measured biomarkers and their intended usage; contemporary regulatory requirements for biomarker assays (a thorough understanding of which is essential to producing a successful and defendable submission); and the technical challenges of analyzing biomarkers produced inside a living organism or cell. Covers the theory of and applications for state-of-the-art mass spectrometry and chromatography and their applications in biomarker analysis Features real-life examples illustrating the challenges involved in target biomarker quantitation and the innovative approaches which have been used to overcome those challenges Addresses potential obstacles to obtain effective biomarker level and data interpretation, such as specificity establishment and sample collection Outlines a tiered approach and fit-for-purpose assay protocol for target biomarker quantitation Highlights the current state of the biomarker regulatory environment and protocol standards Target Biomarker Quantitation by LC-MS is a valuable resource for bioanalytical scientists, drug metabolism and pharmacokinetics scientists, clinical scientists, analytical chemists, and others for whom biomarker quantitation is an important tool of the trade. It also functions as an excellent text for graduate courses in pharmaceutical, biochemistry and chemistry. |
| amino acid analysis by lc-ms/ms: Organic Analysis John Mitchell, Izaak Maurits Kolthoff, Erich S. Proskauer, 1953 |
| amino acid analysis by lc-ms/ms: Handbook of LC-MS Bioanalysis Wenkui Li, Jie Zhang, Francis L. S. Tse, 2013-09-03 Consolidates the information LC-MS bioanalytical scientists need to analyze small molecules and macromolecules The field of bioanalysis has advanced rapidly, propelled by new approaches for developing bioanalytical methods, new liquid chromatographic (LC) techniques, and new mass spectrometric (MS) instruments. Moreover, there are a host of guidelines and regulations designed to ensure the quality of bioanalytical results. Presenting the best practices, experimental protocols, and the latest understanding of regulations, this book offers a comprehensive review of LC-MS bioanalysis of small molecules and macromolecules. It not only addresses the needs of bioanalytical scientists working on routine projects, but also explores advanced and emerging technologies such as high-resolution mass spectrometry and dried blood spot microsampling. Handbook of LC-MS Bioanalysis features contributions from an international team of leading bioanalytical scientists. Their contributions reflect a review of the latest findings, practices, and regulations as well as their own firsthand analytical laboratory experience. The book thoroughly examines: Fundamentals of LC-MS bioanalysis in drug discovery, drug development, and therapeutic drug monitoring The current understanding of regulations governing LC-MS bioanalysis Best practices and detailed technical instructions for LC-MS bioanalysis method development, validation, and stability assessment of analyte(s) of interest Experimental guidelines and protocols for quantitative LC-MS bioanalysis of challenging molecules, including pro-drugs, acyl glucuronides, N-oxides, reactive compounds, and photosensitive and autooxidative compounds With its focus on current bioanalytical practice, Handbook of LC-MS Bioanalysis enables bioanalytical scientists to develop and validate robust LC-MS assay methods, all in compliance with current regulations and standards. |
| amino acid analysis by lc-ms/ms: Clinical Applications of Mass Spectrometry in Biomolecular Analysis Uttam Garg, 2015-11-25 This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and no need for specialized reagents. The techniques described are a must for the measurement of many clinically relevant analytes in the fields of drug analysis, endocrinology, and inborn errors of metabolism. Each chapter provides a brief introduction about a specified analyte, followed by detailed instructions on the analytical protocol. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols is a great resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories. |
| amino acid analysis by lc-ms/ms: Clinical Metabolomics Martin Giera, 2018 This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion. |
| amino acid analysis by lc-ms/ms: LC-MS/MS in Proteomics Pedro R. Cutillas, John F. Timms, 2011-04-06 With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem. |
| amino acid analysis by lc-ms/ms: Biological Mass Spectrometry A.L. Burlingame, 2005-11-28 Describes and integrates the techniques of many advances in both chromatographic and mass spectrometric technologies. This book also covers various biophysical applications, such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. It also describes atto-to-zepto-mole quantitation of 14C and 3H. |
| amino acid analysis by lc-ms/ms: Long-lived Proteins in Human Aging and Disease Roger J. W. Truscott, 2021-04-19 This authoritative overview on an emerging topic in the molecular life sciences covers all aspects of the aging of (long-lived) proteins. It describes the molecular mechanisms of aging on the protein level, in particular the most common side chain modifications and includes analytical methods to study protein half-life and the accumulation of modifications. Finally, the impact of protein aging on several age-related disases in humans is dissected, and their role in limiting human lifespan is discussed. |
| amino acid analysis by lc-ms/ms: Chemistry and Biochemistry of the Amino Acids Graham Barrett, 2012-12-06 Amino acids are featured in course syllabuses and in project and research work over a wide spectrum of subject areas in chemistry and biology. Chemists and biochemists using amino acids have many common needs when they turn to the literature for comprehensive information. Among these common interests, analytical studies, in particular, have undergone rapid development in recent years. All other chemical and biochemical aspects of amino acids - synthesis, properties and reactions, preparation of derivatives for use in peptide synthesis, racemization and other fundamental mechanistic knowledge - have been the subject of vigorous progress. This book offers a thorough treatment of all these developing areas, and is structured in the belief that biochemists, physiologists and others will profit from access to information on topics such as the physical chemistry of amino acid solutions, as well as from thorough coverage of amino acid metabolism, biosynthesis and enzyme inhibition; and that chemists will find relevant material in biological areas as well as in the analysis, synthesis and reactions of amino acids. |
| amino acid analysis by lc-ms/ms: Peptidomics Mikhail Soloviev, Per Andrén, Chris Shaw, 2007-12-21 The definitive guide to peptidomics- a hands-on lab reference The first truly comprehensive book about peptidomics for protein and peptide analysis, this reference provides a detailed description of the hows and whys of peptidomics and how the techniques have evolved. With chapters contributed by leading experts, it covers naturally occurring peptides, peptidomics methods and new developments, and the peptidomics approach to biomarker discovery. Explaining both the principles and the applications, Peptidomics: Methods and Applications: * Features examples of applications in diverse fields, including pharmaceutical science, toxicity biomarkers, and neuroscience * Details the successful peptidomic analyses of biological material ranging from plants to mammals * Describes a cross section of analytical techniques, including traditional methodologies, emerging trends, and new techniques for high throughput approaches An enlightening reference for experienced professionals, this book is sufficiently detailed to serve as a step-by-step guide for beginning researchers and an excellent resource for students taking biotechnology and proteomics courses. It is an invaluable reference for protein chemists and biochemists, professionals and researchers in drug and biopharmaceutical development, analytical and bioanalytical chemists, toxicologists, and others. |
| amino acid analysis by lc-ms/ms: Sample Preparation in LC-MS Bioanalysis Wenkui Li, Wenying Jian, Yunlin Fu, 2019-03-12 Revised and Expanded Handbook Provides Comprehensive Introduction and Complete Instruction for Sample Preparation in Vital Category of Bioanalysis Following in the footsteps of the previously published Handbook of LC-MS Bioanalysis, this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography–Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies used in human healthcare. While advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include: Current basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, microsampling, sample extraction via electromembranes) Sample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor) Crucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivation strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates) Sample Preparation in LC-MS Bioanalysis will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring. |
| amino acid analysis by lc-ms/ms: Biochemistry of Smooth Muscle Contraction Michael Barany, 1996-01-04 This valuable resource provides a systematic account of the biochemistry of smooth muscle contraction. As a comprehensive guide to this rapidly growing area of research, it covers the structure and characteristic properties of contractile and regulatory proteins, with special emphasis on their predicted function in the live muscle. Also included in this book are intermediate filament proteins, and desmin and vimentin, whose function in smooth muscle is unknown; and several enzymes involved in the phosphorylation-dephosphorylation of contractile and other proteins. |
| amino acid analysis by lc-ms/ms: Amino Acids and Derivatives B. J. Miflin, 1980 |
| amino acid analysis by lc-ms/ms: Vademecum Metabolicum Johannes Zschocke, Georg F. Hoffmann, 2011 |
| amino acid analysis by lc-ms/ms: Plant Metabolomics Kazuki Saito, Richard A. Dixon, Lothar Willmitzer, 2006-06-29 Metabolomics – which deals with all metabolites of an organism – is a rapidly-emerging sector of post-genome research fields. It plays significant roles in a variety of fields from medicine to agriculture and holds a fundamental position in functional genomics studies and their application in plant biotechnology. This volume comprehensively covers plant metabolomics for the first time. The chapters offer cutting-edge information on analytical technology, bioinformatics and applications. They were all written by leading researchers who have been directly involved in plant metabolomics research throughout the world. Up-to-date information and future developments are described, thereby producing a volume which is a landmark of plant metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all plant science fields. |
| amino acid analysis by lc-ms/ms: Proteomics Sample Preparation Jörg von Hagen, 2011-08-24 This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task. |
| amino acid analysis by lc-ms/ms: Quantitation of Amino Acids and Amines by Chromatography Ibolya Molnár-Perl, 2005-06-27 Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols is intended to serve as a ready-to-use guide for the identification and quantification of amino acids and amines in various matrices, providing an overview on the theory and protocol of available methods. It presents chromatograms with exact elution programs enabling visual analysis and compares the advantages-disadvantages of various chromatographic techniques. In accordance with the chronological order of the development of chromatographic methods, different techniques are discussed: The possibilities of gas chromatography (GC), followed by those of the high performance liquid chromatography (HPLC) and the most recent techniques capillary electrophoresis (CE), capillary, electrochromatography (CEC).The characteristics of the given chromatographic procedure, relating to the topic in question, are classified according to the preliminary preparation/derivatization process(es), which means the simple methods, suitable for the analysis of the selected compound(s) in natural form, are followed by various derivatization proposals. Detailed protocols provide the reader with guidance in beginning tasks and on how to improve current methods. This book appeals to a wide audience and is recommended for those looking towards the wider reaches of identification and quantification of amino acids and amines.* Provides a systematic, and comprehensive summary of chromatographic techniques and derivatization processes* Compares advantages/disadvantages of various chromatographic techniques* Readers can undertake practical tasks using detailed protocols given in the book |
| amino acid analysis by lc-ms/ms: Mass Spectrometry in Biophysics Igor A. Kaltashov, Stephen J. Eyles, 2005-05-06 The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical background and technical aspects of eachmethod, this much-needed reference offers an unparalleled overviewof the current state of biophysical MS. Mass Spectrometry inBiophysics begins with a helpful discussion of general biophysicalconcepts and MS-related techniques. Subsequent chaptersaddress: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-basedmethods * Techniques used to study structure and behavior of non-nativeprotein states that become populated under denaturingconditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information onprotein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physicsof electrospray mass spectrometry, Mass Spectrometry in Biophysicsalso offers a compelling look at the current challenges facingbiomolecular MS and the potential applications that will likelyshape its future. |
| amino acid analysis by lc-ms/ms: Analyzing Biomolecular Interactions by Mass Spectrometry Jeroen Kool, Wilfried M. A. Niessen, 2015-05-04 This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories. |
| amino acid analysis by lc-ms/ms: LC-MS in Drug Analysis Loralie J. Langman, Christine L. H. Snozek, 2012-07-06 Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance. The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques. The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies. |
| amino acid analysis by lc-ms/ms: Applications of Ion Exchange Materials in Biomedical Industries Inamuddin, 2019-01-30 This book presents the applications of ion-exchange materials in the biomedical industries. It includes topics related to the application of ion exchange chromatography in determination, extraction and separation of various compounds such as amino acids, morphine, antibiotics, nucleotides, penicillin and many more. This title is a highly valuable source of knowledge on ion-exchange materials and their applications suitable for postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology. Additionally, this book will provide an in-depth knowledge of ion-exchange column and operations suitable for engineers and industrialists. |
| amino acid analysis by lc-ms/ms: Essentials of Glycobiology Ajit Varki, Maarten J. Chrispeels, 1999 Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. Essentials of Glycobiology describes their biogenesis and function and offers a useful gateway to the understanding of glycans. |
| amino acid analysis by lc-ms/ms: Plant Lipid Metabolism J.C. Kader, Paul Mazliak, 2013-04-18 A collection of papers that comprehensively describe the major areas of research on lipid metabolism of plants. State-of-the-art knowledge about research on fatty acid and glycerolipid biosynthesis, isoprenoid metabolism, membrane structure and organization, lipid oxidation and degradation, lipids as intracellular and extracellular messengers, lipids and environment, oil seeds and gene technology is reviewed. The different topics covered show that modern tools of plant cellular and molecular biology, as well as molecular genetics, have been recently used to characterize several key enzymes of plant lipid metabolism (in particular, desaturases, thioesterases, fatty acid synthetase) and to isolate corresponding cDNAs and genomic clones, allowing the use of genetic engineering methods to modify the composition of membranes or storage lipids. These findings open fascinating perspectives, both for establishing the roles of lipids in membrane function and intracellular signalling and for adapting the composition of seed oil to the industrial needs. This book will be a good reference source for research scientists, advanced students and industrialists wishing to follow the considerable progress made in recent years on plant lipid metabolism and to envision the new opportunities offered by genetic engineering for the development of novel oil seeds. |
| amino acid analysis by lc-ms/ms: Mass Spectrometry Handbook Mike S. Lee, 2012-04-16 Due to its enormous sensitivity and ease of use, mass spectrometry has grown into the analytical tool of choice in most industries and areas of research. This unique reference provides an extensive library of methods used in mass spectrometry, covering applications of mass spectrometry in fields as diverse as drug discovery, environmental science, forensic science, clinical analysis, polymers, oil composition, doping, cellular research, semiconductor, ceramics, metals and alloys, and homeland security. The book provides the reader with a protocol for the technique described (including sampling methods) and explains why to use a particular method and not others. Essential for MS specialists working in industrial, environmental, and clinical fields. |
| amino acid analysis by lc-ms/ms: A Practical Guide to Protein and Peptide Purification for Microsequencing Paul T. Matsudaira, 2012-12-02 Why a Second Edition?The Second Edition provides practical answers to the general question, How can I obtain useful sequence information from my protein or peptide? rather than the more specific question asked in the first edition, How can I obtain the N-terminal sequence? Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing |
| amino acid analysis by lc-ms/ms: Amino Acid Analysis Protocols Catherine Cooper, 2000-09-05 A collection of classic and cutting-edge techniques of high utility in answering specific biological questions about amino acids. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Each method is described in step-by-step detail to ensure successful experimental results and emphasizes sample preparation, particularly the collection and storage of bodily fluids. Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids. |
| amino acid analysis by lc-ms/ms: Chiral Separations Gerald Gübitz, Martin G. Schmid, 2008-02-02 Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques. |
| amino acid analysis by lc-ms/ms: Proteomics for Food Authentication Leo M.L. Nollet, Semih Ötleş, 2020-05-07 Consumers have the right to know what is in the food they are eating, and accordingly, a number of global food regulations require that the provenance of the food can be guaranteed from farm to fork. Many different instrumental techniques have been proposed for food authentication. Although traditional methods are still being used, new approaches such as genomics, proteomics, and metabolomics are helping to complement existing methodologies for verifying the claims made about certain food products. During the last decade, proteomics (the largescale analysis of proteins in a particular biological system at a particular time) has been applied to different research areas within food technology. Since proteins can be used as markers for many properties of a food, even indicating processes to which the food has been subjected, they can provide further evidence of the foods labeling claim. Proteomics for Food Authentication, a volume in the Food Analysis and Properties Series, is a comprehensive and updated overview of the applications, drawbacks, advantages, and challenges of proteomics for food authentication. Features: Provides a comprehensive and critical overview of the application of proteomics in food Helps food scientists determine the authenticity of several food products Provides applied techniques for both laboratory and industrial environments Describes workflows, technologies, and tools that are being assessed in proteomics-related studies Workflows, technologies, and tools that are being assessed in proteomics-related studies are described, followed by a review of the specific applications regarding food authenticity and, now and then, food quality. The book will provide a comprehensive and critical overview of the application of proteomics approaches to determine the authenticity of several food products updating the performances and current limitations of the applied techniques in both laboratory and industrial environments. As such it is well suited to food scientist, chemical engineers, food engineers, research labs, universities, governments, related food industries. Also available in the Food Analysis and Properties Series: Food Aroma Evolution: During Food Processing, Cooking, and Aging, edited by Matteo Bordiga and Leo M.L. Nollet (ISBN: 9781138338241) Ambient Mass Spectroscopy Techniques in Food and the Environment, edited by Leo M.L. Nollet and Basil K. Munjanja (ISBN: 9781138505568) Hyperspectral Imaging Analysis and Applications for Food Quality, edited by N.C. Basantia, Leo M.L. Nollet, and Mohammed Kamruzzaman (ISBN: 9781138630796) For a complete list of books in this series, please visit our website at: www.crcpress.com/Food-Analysis--Properties/book-series/CRCFOODANPRO |
| amino acid analysis by lc-ms/ms: Non-Proteinogenic Amino Acids , 2018 |
| amino acid analysis by lc-ms/ms: Contemporary Practice in Clinical Chemistry William Clarke, Mark Marzinke, 2020-06-11 Contemporary Practice in Clinical Chemistry, Fourth Edition, provides a clear and concise overview of important topics in the field. This new edition is useful for students, residents and fellows in clinical chemistry and pathology, presenting an introduction and overview of the field to assist readers as they in review and prepare for board certification examinations. For new medical technologists, the book provides context for understanding the clinical utility of tests that they perform or use in other areas in the clinical laboratory. For experienced laboratorians, this revision continues to provide an opportunity for exposure to more recent trends and developments in clinical chemistry. - Includes enhanced illustration and new and revised color figures - Provides improved self-assessment questions and end-of-chapter assessment questions |
| amino acid analysis by lc-ms/ms: Mass Spectrometry for the Clinical Laboratory Hari Nair, William Clarke, 2016-11-02 Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to meet CLIA requirements, appropriate interference testing, measuring, validation of ion suppression/matrix effects, and quality control. These tools offer guidance on what type of instrumentation is optimal for each assay, what options are available, and the pros and cons of each. Readers will find a full set of tools that are either directly related to the assay they want to adopt or for an analogous assay they could use as an example. Written by expert users of the most common assays found in a clinical laboratory (clinical chemists, toxicologists, and clinical pathologists practicing mass spectrometry), the book lays out how experts in the field have chosen their mass spectrometers, purchased, installed, validated, and brought them on line for routine testing. The early chapters of the book covers what the practitioners have learned from years of experience, the challenges they have faced, and their recommendations on how to build and validate assays to avoid problems. These chapters also include recommendations for maintaining continuity of quality in testing. The later parts of the book focuses on specific types of assays (therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has been written by an expert practitioner of an assay that is currently running in his or her clinical lab. Provides readers with the keys to choosing, installing, and validating a mass spectrometry platform Offers tools to evaluate, validate, and troubleshoot the most common assays seen in clinical pathology labs Explains validation, ion suppression, interference testing, and quality control design to the detail that is required for implementation in the lab |
| amino acid analysis by lc-ms/ms: Validating Chromatographic Methods David M. Bliesner, 2006-09-11 All the information and tools needed to set up a successful method validation system Validating Chromatographic Methods brings order and Current Good Manufacturing Practices to the often chaotic process of chromatographic method validation. It provides readers with both the practical information and the tools necessary to successfully set up a new validation system or upgrade a current system to fully comply with government safety and quality regulations. The net results are validated and transferable analytical methods that will serve for extended periods of time with minimal or no complications. This guide focuses on high-performance liquid chromatographic methods validation; however, the concepts are generally applicable to the validation of other analytical techniques as well. Following an overview of analytical method validation and a discussion of its various components, the author dedicates a complete chapter to each step of validation: Method evaluation and further method development Final method development and trial method validation Formal method validation and report generation Formal data review and report issuance Templates and examples for Methods Validation Standard Operating Procedures, Standard Test Methods, Methods Validation Protocols, and Methods Validation Reports are all provided. Moreover, the guide features detailed flowcharts and checklists that lead readers through every stage of method validation to ensure success. All of the templates are also included on a supplementary support site, enabling readers to easily work with and customize them. For scientists and technicians new to method validation, this guide provides all the information and tools needed to develop a top-quality system. For those experienced with method validation, the guide helps to upgrade and improve existing systems. |
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Separation of an Amino Acid Mixture Using a Kinetex XB-C18 …
HPLC is a well characterized method for amino acid analysis, LC-MS/MS analysis of amino acids poses several challenges. In this application note, a Kinetex 2.6 µm XB-C18 column and a …
LC/MS Based Characterization of GLP-1 Therapeutic Peptide …
describes a workflow for LC/MS analysis of liraglutide and its impurities, using reversed-phase chromatography combined with Q-TOF MS/MS detection. Liraglutide is a full agonist of the …
National Institute of Standards & Technology Certificate of …
Measurement of BSA C oncentration by Amino Acid Analysis (ID-LC/MS/MS): The amino acid analysis method involved isotope dilution liquid chromatography/tandem mass spectrometry …
Quantitative amino acids analysis for the diagnosis and …
Aug 15, 2016 · MS/MS . GC-MS . UPLC . IP-LC-MS/MS . IP-LC-MS/MS . using TRAQ reagents . Anal Bioanal Chem (2009) 393:445452– • Isomers/isobars cannot be resolved • Not suitable …
The Analysis of Underivatized Amino Acids by HPLC with MS …
The analysis of amino acids plays an important . role in a wide range of applications, including those in food, beverage and biomedical industries. A common method for amino acid analysis …
EZ:Faast AMINO ACID ANALYSIS - AmXpress
AMINO ACID ANALYSIS TM EZ:Faast™ AMINO ACID ANALYSIS LC/MS Kit By taking the technology developed with the EZ:faast GC kit and applying it to LC/MS analysis, creation of …
0004377042 253..261 - Springer
chromatography-tandem mass spectrometry (LC-MS/MS). The method allows the simultaneous analysis of 18 D-amino acids with high sensitivity and reproducibility. Additionally, this chapter …
Underivatized amino acid analysis in wine by HILIC …
accurate analysis of both amino acid composition of wines and changes in amino acid content during wine manufacturing and aging. Proline is the most abundant amino acid in wine, …
Lc-MS Analysis of Amino Acids on a Novel Mixed-Mode …
demonstrated. Amino acid samples of varying complexity and from various matrices, including isomers, dipeptides, and related compounds are separated using optimized methods spanning …
Development of a novel amino acids analysis column for LC …
This novel HPLC method will be a powerful tool for amino acid LC-MS analysis in many different biochemistry applications. Described in each figure. METHOD RESULTS AND DISCUSSION …
Quantitative LC-MS Solution for Targeted Analysis of Cell
MIDAS™workflow. As shown, the identification of each isomer is based on MS/MS spectral matching between the experimental and theoretical MS/MS spectra from the AMMSL library. In …
Chapter 15 Ch - Springer
15 Amino Acid Analysis in Physiological Samples by GC MS 167 (iTRAQ) technology offered by AB SCIEX (Foster City , CA, USA). Originally developed for isotope labeling of peptides ( 10 ) , …
어류 중 아미노산 분석을 위한 비-유도체화 LC-MSMS 분석법 개발
어류 중 아미노산 분석을 위한 비-유도체화 LC-MS/MS 분석법 개발 아미노산 (Amino Acid, 이하 AA)은 건강과 활력을 유지하는 데 도움을 주는 신진 대사에서의 단백질과 중간체의 구성 요소로써 …
Solutions for Clinical Research - Shimadzu
Integration of Amino Acid, Acylcarnitine and Steroids Analysis in Single FIA/LC-MS/MS Platform In this study, we present a strategy for performing both amino acids (AA)/ acylcarnitines (AC) …
Amino Acid iological Fluids L-MS/MS Analysis Kit User …
Quantitative analysis of the amino acids in biological fluids also has clinical importance for determining some neurological and endocrinal disorders. LC-MS/MS is an influential device for …
Protein Sequencing Using High Resolution LC-MS/MS
Schematic representation of sample preparation for LC-MS/MS analysis. Non-reduced sample, LC/MS/MS analysis. Ovalbumin. Reduced/alkylated/ deglycosylated sample, LC/MS/MS …
Chiral Analysis of Amino Acids in Bacterial Samples Using …
1.8 Literature Results based on Studies Done for Amino Acid Analysis by LC-MS/MS. 1.9 Goals 2.0 Experimental 2.1 Chemicals 2.2 Gases and Solvents 2.3 Samples for Analysis 2.3.1 …
Determination of free amino acids in plants by liquid …
on the application of EZ:faast™ and LC-MS/MS for the analysis of AAs in plants. ... reagents were included in the EZ:faast™ LC-MS Free Amino Acid kit (Phenomenex, Torrance, CA, USA). The …
Analysis of Amino Acids by HPLCAnalysis of Amino Acids by …
Agilent’s Solutions for Amino Acid Analysis A Closer LookA Closer Look Agilent has one basic type of chemistry for amino acid analysis – OPA/FMOC derivatization. We have 3 columns and …
Note: 370 AminoAcid Derivatives in Plasma - Thermo Fisher …
Amino acids and their derivatives are important biomarkers in human physiological processes. Their identification and quantitation can provide significant insights related with human …
LC-MS/MS Strategies for Impurity profiling of Peptide API …
• Leu and Ile can be differentiated via fragmentation of immonium ions generated by source CID in a single bottom-up LC-MS/MS sequence confirmation run. • Isoglutamic acid and Isoaspartic …
Development of Precolumn Derivatization–LC/MS for …
amino acid concentrations in plasma is useful for risk screening of diseases including cancer, diabetes, and myocardial infarction. This functional-group-targeted analysis through LC/MS for …
USER’S MANUAL - Phenomenex
ies for most amino acid derivatives as run by the EZ:faast methodology. Spectral libraries are included for Varian Saturn™ and Agilent Chemstation software systems. Table 1 - …
Simultaneous Analysis of Amino Acids and Acylcarnitines in …
LC/MS and an Agilent 1200 Series G1367A Well-Plate Autosampler. The instrument conditions are listed in Table 2. Sample preparation ... is characteristic for each amino acid. The analysis …
Quantitative LC-MS Solution for Targeted Analysis of Cell
new CCM method targets important nutrients, including amino acids, vitamins, carbohydrates, fatty acids, nucleic acid, inorganic acids, and other essential compounds found in media. By …
Analysis of Amino Acids in Animal Feed Matrices Using the
for the quantitation of underivatized amino acids using an Agilent Ultivo triple quadrupole LC/MS system. Ultivo was designed to address many challenges faced by laboratories, and this study …
CLAMä LCMS Application Measuring Primary Metabolites in …
Plasma Using an LC/MS/MS System with Fully Automated Sample Preparation Module Capable of comprehensive quantitative analysis of primary metabolites in plasma
Separation of an Amino Acid Mixture in HILIC Mode Using a …
HPLC is a well characterized method for amino acid analysis, LC-MS/MS analysis of amino acids poses several challenges. Some compounds are very polar and are hard to retain by reversed …
USERGUIDE - .NET Framework
may find traditional amino acid analysis laborious and costly. EZ:faast Amino Acid analysis kits has been developed to overcome these obstacles with a simple kit containing reagents and …
Inborn errors of metabolism (IEM) - Quest Diagnostics
29881 Amino Acid Analysis, LC/MS, CSF1 767(X) Amino Acid Analysis, LC/MS, Plasma 1 36183(X) Amino Acid Analysis, LC/MS, Urine1 34694 Arylsulfatase A1 70132(X) Biotinidase1 …
Simultaneous Analysis of Water-soluble and Fat-soluble …
Simultaneous Analysis of Water-soluble and Fat-soluble Vitamins in Fish by Reversed-Phase LC-MS/MS Method A detection and quantitation method for 8 FSV and 9 WSV in ˜sh extract was …
C146-E336 LC/MS/MS Method Package for D/L Amino Acids
With conventional chiral amino acid analysis, it is necessary to perform derivatization or use very long run times. With this method package, derivatization is not necessary, and high-sensitivity …
Quantitative Analysis ASMS 2008 of Underivatized Glutamine, …
Quantitative Analysis of Underivatized Glutamine, Glutamic Acid, Asparagine, and Aspartic Acid in Cell Media using LC/MS/MS Glutamine (Gln), glutamic acid (Glu), Asparagine (Asn), and …
Certification of NIST standard reference material 2389a, …
the LC-MS/MS measurements with gravimetric preparation values agreed to within |1.1|% for all amino acids. NIST SRM 2389a will be available for characterization of routine methods for …
Improved and Simplified Liquid …
The amino acids were extracted from the matrixes using acidified water. Simul-taneous determination of 22 underivatized amino acids was carried out by liquid chromatography–mass …
LC/MS/MS Method Package for Sugars and Sugar Nucleotides
other substances within the body. The LC/MS/MS Method Package for Sugars and Sugar Nucleotides includes LC/MS/MS analytical methods with analytical conditions and MS …
Targeted Quantification of Cell Culture Media Components …
An LC-MS/MS method library was developed for the quantitative analysis of components in cell culture medium and spent cell culture medium. This method, which covers a wide range of …
Simplification of FDLA Pre-Column Derivatization for …
d,l-amino acids, LC/MS/MS analysis for the simultaneous enantioseparation of 20 proteinogenic amino acids derivat-ized with FDLA has not been reported yet. Therefore, we next examined …
Determination of inborn errors of metabolism - Thermo Fisher …
Table 2. LC method description Table 3. MS settings Table 3. MS settings Amino acids Amino acid internal standards Acylcarnitines Acylcarnitine internal standards Alanine d 4-Alanine …
Selective and highly accurate analysis of desmopressin …
Vanquish, Vanquish, desmopressin, LC-MS/MS, UHPLC, mixed-mode SPE, solid-phase extraction, micro-elution, peptides, bioanalysis, biopharma Goal To describe an accurate, …
A Practical Method for Amino Acid Analysis by LC-MS Using …
spectrometry (LC-MS) has become an essential analytical tool for separating AAs [13]. The structure of the analyte can be determined using MS, making the detection of AAs more …
Chapter 14
Amino Acid Analysis in Physiological Samples by GC-MS with Propyl Chloroformate Derivatization and iTRAQ-LC-MS/MS Katja Dettmer, Axel P. Stevens, Stephan R. Fagerer, …
Amino Acid Analysis of Pure Protein Hydrolysates with …
Amino acid analysis is used in the protein structure laboratory to provide two kinds of information. First, the ... LC conditions LC system: ACQUITY UPLC System with TUV detection at 260 …
QUANTITATIVE LC-MS/MS Analysis of proteins and peptides
LC-MS method optimization & characterization • Linear calibration curve • Limit of detection (LOD) –3:1 signal-to-noise ratio • Limit of quantitation (LOQ) –10:1 signal-to-noise ratio • Sample …
Tissue and plasma free amino acid detection by LC-MS/MS …
developed for estimating the risk of cancer by rapid analysis of plasma free amino acid (PFAA) levels. There is scarce evi- ... U-glutamic acid and valine amino acid levels by LC-MS/MS …
MSU_MSMC_002 Free Amino acid extraction
Mar 19, 2019 · 7) Store samples at -20˚ C (or -80˚ C) until ready to analyze by LC/MS/MS Amino acid standard curve 1) Prepare a stock solution of each unlabeled amino acid at 5 mM in water …
Rapid Analysis of Genotoxic Nitrosamines - SCIEX
were transferred to HPLC vials for analysis by LC-MS/MS. Standards are prepared by diluting mixtures of the eight compound’s working standards into the extraction solvent to final …
Amino Acid Analysis of Mammalian Cell Culture Medium by …
• ™Thermo Scientific™ Pierce Amino Acid Standard H, hydrolysates standard, 10 × 1 mL ampules (P/N 20088) Chemicals for chromatography • Deionized water, 18.2 MΩ·cm resistivity or higher …
quantitation of free amino acids in human plasma by single …
Liquid chromatography triple quadrupole mass spectrometry (LC-MS/MS) analysis of amino acids is becoming the method of choice by more and more laboratories because of its speed, …
iMethod Test Amino Acid Analysis - SCIEX
Test for the analysis of 45 Amino Acids in physiological fluid using amino reactive isotope coded tags in conjunction with LC/MS/MS. This method has been verified for use on both AB SCIEX …
