Advertisement
| amicon ultra 0.5 ml manual: Pseudokinases , 2022-05-05 Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Includes the latest information on Pseudokinases |
| amicon ultra 0.5 ml manual: Methods in Biotechnology Seung-Beom Hong, M. Bazlur Rashid, Lory Z. Santiago-Vázquez, 2016-08-01 As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level – Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field. |
| amicon ultra 0.5 ml manual: Experiments in the Purification and Characterization of Enzymes Thomas E. Crowley, Jack Kyte, 2014-01-11 Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. - Offers project lab formats for students that closely simulate original research projects - Provides instructional guidance for students to design their own experiments - Includes advanced analytical techniques - Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH - Includes access to a website with additional resources for instructors |
| amicon ultra 0.5 ml manual: Biophysical Chemistry of Proteins Engelbert Buxbaum, 2010-11-18 The book is structured in nine sections, each containing several chapters. The volume starts with an overview of analytical techniques and progresses through purification of proteins; protein modification and inactivation; protein size, shape, and structure; enzyme kinetics; protein-ligand interactions; industrial enzymology; and laboratory quality control. The book is targeted at all scientists interested in protein research. |
| amicon ultra 0.5 ml manual: High Energy Conformations in Protein Folding and Binding David Edward Wildes, 2006 |
| amicon ultra 0.5 ml manual: Human Stem Cell Manual Suzanne Peterson, Jeanne F. Loring, 2012-10-22 This manual is a comprehensive compilation of methods that work for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. - Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications - Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America - Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs |
| amicon ultra 0.5 ml manual: Peptidomics Michael Schrader, |
| amicon ultra 0.5 ml manual: Nanomaterial Characterization Ratna Tantra, 2016-04-04 Nanomaterial Characterization Providing various properties of nanomaterials and the various methods available for their characterization Over the course of the last few decades, research activity on nanomaterials has gained considerable press coverage. The use of nanomaterials has meant that consumer products can be made lighter, stronger, esthetically more pleasing, and less expensive. The significant role of nanomaterials in improving the quality of life is clear, resulting in faster computers, cleaner energy production, target-driven pharmaceuticals, and better construction materials. It is not surprising, therefore, that nanomaterial research has really taken off, spanning across different scientific disciplines from material science to nanotoxicology. A critical part of any nanomaterial research, however, is the need to characterize physicochemical properties of the nanomaterials, which is not a trivial matter. Nanomaterial Characterization: An Introduction is dedicated to understanding the key physicochemical properties and their characterization methods. Each chapter begins by giving an overview of the topic before a case study is presented. The purpose of the case study is to demonstrate how the reader may make use of the background information presented to them and show how this can be translated to solve a nanospecific application scenario. Thus, it will be useful for researchers in helping them design experimental investigations. The book begins with a general overview of the subject, thus giving the reader a solid foundation to nanomaterial characterization. Nanomaterial Characterization: An Introduction features: Nanomaterial synthesis and reference nananomaterials Key physicochemical properties and their measurements including particle size distribution by number, solubility, surface area, surface chemistry, mechanical/tribological properties, and dustiness Scanning tunneling microscopy methods operated under extreme conditions Novel strategy for biological characterization of nanomaterial methods Methods to handle and visualize multidimensional nanomaterial characterization data The book is written in such a way that both students and experts in other fields of science will find the information useful, whether they are in academia, industry, or regulation, or those whose analytical background may be limited.There is also an extensive list of references associated with every chapter to encourage further reading. |
| amicon ultra 0.5 ml manual: Laboratory Methods Manual for Hormone Action and Molecular Endocrinology , 1986 |
| amicon ultra 0.5 ml manual: Forensic DNA Biology Kelly M. Elkins, 2012-08-03 DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies will be used throughout. - Provides practical step-by-step instruction on how to perform forensic DNA analysis - Includes analysis of hair, presumptive testing of body fluids, human DNA typing and statistics - Covers techniques such as pipetting, microscopy and DNA extraction - Pre- and post-lab exercises and questions assist the reader in learning the material - Report writing templates assure the reader learns real world crime lab procedure |
| amicon ultra 0.5 ml manual: Mass Spectrometry-Based Proteomics Kris Gevaert, 2023-09-04 This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters on automated preparation of clinical samples, the analysis of formalin-fixed paraffin-embedded samples, protocols for the isolation of extracellular vesicles and for the monitoring of selected protein modifications in clinical samples, and, finally, structural proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Proteomics serves as an ideal guide to its subject for both novices in the field of proteomics as well as specialists. |
| amicon ultra 0.5 ml manual: Serum/Plasma Proteomics Richard J Simpson, David W Greening, 2016-05-01 Blood science has become a cornerstone of multiple disciplines. This book, contributed to by leading experts in the field, provides a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. |
| amicon ultra 0.5 ml manual: Asymmetric and Selective Biocatalysis Jose M. Palomo, Cesar Mateo, 2019-04-12 This Issue contains one communication, six articles, and two reviews. The communication from Paola Vitale et al. represents a work where whole cells were used as biocatalysts for the reduction of optically active chloroalkyl arylketones followed by a chemical cyclization to give the desired heterocycles. Among the various whole cells screened (baker’s yeast, Kluyveromyces marxianus CBS 6556, Saccharomyces cerevisiae CBS 7336, Lactobacillus reuteri DSM 20016), baker’s yeast provided the best yields and the highest enantiomeric ratios (95:5) in the bioreduction of the above ketones. In this respect, valuable chiral non-racemic functionalized oxygen-containing heterocycles (e.g., (S)-styrene oxide, (S)-2-phenyloxetane, (S)-2-phenyltetrahydrofuran), amenable to be further elaborated on, can be smoothly and successfully generated from their prochiral precursors. Studies about pure biocatalysts with mechanistical studies, application in different reactions, and new immobilization methods for improving their stability were reported in five different articles. The article by Su-Yan Wang et al. describes the cloning, expression, purification, and characterization of an N-acetylglucosamine 2-epimerase from Pedobacter heparinus (PhGn2E). For this, several N-acylated glucosamine derivatives were chemically synthesized and used to test the substrate specificity of the enzyme. The mechanism of the enzyme was studied by hydrogen/deuterium NMR. The study at the anomeric hydroxyl group and C-2 position of the substrate in the reaction mixture confirmed the epimerization reaction via ring-opening/enolate formation. Site-directed mutagenesis was also used to confirm the proposed mechanism of this interesting enzyme. The article by Forest H. Andrews et al. studies two enzymes, benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC), which catalyze the non-oxidative decarboxylation of 2-keto acids with different specificity. BFDC from Pseudomonas putida exhibited very limited activity with pyruvate, whereas the PDCs from S. cerevisiae or from Zymomonas mobilis showed virtually no activity with benzoylformate (phenylglyoxylate). After studies using saturation mutagenesis, the BFDC T377L/A460Y variant was obtained, with 10,000-fold increase in pyruvate/benzoylformate. The change was attributed to an improvement in the Km value for pyruvate and a decrease in the kcat value for benzoylformate. The characterization of the new catalyst was performed, providing context for the observed changes in the specificity. The article by Xin Wang et al. compares two types of biocatalysts to produce D-lysine L-lysine in a cascade process catalyzed by two enzymes: racemase from microorganisms that racemize L-lysine to give D,L-lysine and decarboxylase that can be in cells, permeabilized cells, and the isolated enzyme. The comparison between the different forms demonstrated that the isolated enzyme showed the higher decarboxylase activity. Under optimal conditions, 750.7 mmol/L D-lysine was finally obtained from 1710 mmol/L L-lysine after 1 h of racemization reaction and 0.5 h of decarboxylation reaction. D-lysine yield could reach 48.8% with enantiomeric excess (ee) of 99%. In the article by Rivero and Palomo, lipase from Candida rugosa (CRL) was highly stabilized at alkaline pH in the presence of PEG, which permitted its immobilization for the first time by multipoint covalent attachment on different aldehyde-activated matrices. Different covalent immobilized preparation of the enzyme was successfully obtained. The thermal and solvent stability was highly increased by this treatment, and the novel catalysts showed high regioselectivity in the deprotection of per-O-acetylated nucleosides. The article by Robson Carlos Alnoch et al. describes the protocol and use of a new generation of tailor-made bifunctional supports activated with alkyl groups that allow the immobilization of proteins through the most hydrophobic region of the protein surface and aldehyde groups that allows the covalent immobilization of the previously adsorbed proteins. These supports were especially used in the case of lipase immobilization. The immobilization of a new metagenomic lipase (LipC12) yielded a biocatalyst 3.5-fold more active and 5000-fold more stable than the soluble enzyme. The PEGylated immobilized lipase showed high regioselectivity, producing high yields of the C-3 monodeacetylated product at pH 5.0 and 4 °C. Hybrid catalysts composed of an enzyme and metallic complex are also treated in this Special Issue. The article by Christian Herrero et al. describes the development of the Mn(TpCPP)-Xln10A artificial metalloenzyme, obtained by non-covalent insertion of Mn(III)-meso-tetrakis(p-carboxyphenyl)porphyrin [Mn(TpCPP), 1-Mn] into xylanase 10A from Streptomyces lividans (Xln10A). The complex was found able to catalyze the selective photo-induced oxidation of organic substrates in the presence of [RuII(bpy)3]2+ as a photosensitizer and [CoIII(NH3)5Cl]2+ as a sacrificial electron acceptor, using water as oxygen atom source. The two published reviews describe different subjects with interest in the fields of biocatalysis and mix metallic-biocatalysis, respectively. The review by Anika Scholtissek et al. describes the state-of-the-art regarding ene-reductases from the old yellow enzyme family (OYEs) to catalyze the asymmetric hydrogenation of activated alkenes to produce chiral products with industrial interest. The dependence of OYEs on pyridine nucleotide coenzyme can be avoided by using nicotinamide coenzyme mimetics. In the review, three main classes of OYEs are described and characterized. The review by Yajie Wang and Huimin Zhao highlights some of the recent examples in the past three years that combine transition metal catalysis with enzymatic catalysis. With recent advances in protein engineering, catalyst synthesis, artificial metalloenzymes, and supramolecular assembly, there is great potential to develop more sophisticated tandem chemoenzymatic processes for the synthesis of structurally complex chemicals. In conclusion, these nine publications give an overview of the possibilities of different catalysts, both traditional biocatalysts and hybrids with metals or organometallic complexes to be used in different processes—particularly in synthetic reactions—under very mild reaction conditions. |
| amicon ultra 0.5 ml manual: Riboswitch Discovery, Structure and Function , 2014-11-21 This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, and ligand interactions and thermodynamics. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods in riboswitch discovery, structure and function - Contains sections on such topics as riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, ligand interactions and thermodynamics |
| amicon ultra 0.5 ml manual: The Development of Biomarkers in Psychiatry Takashi Nakano, Takahiro A. Kato, Masahiro Takamura, Shin-ichi Kano, 2023-01-03 |
| amicon ultra 0.5 ml manual: Bacteriophages Pilar García Suárez, Lucía Fernández, 2020-11-24 There is talk of an upcoming antibiotic armageddon, with untreatable post-operative infections, and similarly untreatable complications after chemotherapy. Indeed, the now famous “O’Neill Report” (https://amr-review.org/) suggests that, by 2050, more people might die from antibiotic-resistant bacterial infections than from cancer. While we are still learning all the subtle drivers of antibiotic resistance, it seems increasingly clear that we need to take a “one health” approach, curtailing the use of antibiotics in both human and veterinary medicine. However, there are no new classes of antibiotics on our horizon. Maybe something that has been around “forever” can come to our rescue—bacteriophages! Nevertheless, it is also necessary to do things differently, and use these new antimicrobials appropriately. Therefore, an in-depth study of bacteriophage biology and case-by-case applications might be required. Whilst by no means comprehensive, this book does cover some of the many topics related to bacteriophages as antimicrobials, including their use in human therapy and aquaculture. It also explores the potential use of phage endolysins as substitutes of antibiotics in two sectors where there is an urgent need—human therapy and the agro-food industry. Last but not least, there is an excellent perspective article on phage therapy implementation. |
| amicon ultra 0.5 ml manual: Virology Methods Manual Hillar O. Kangro, Brian W.J. Mahy, 1996-04-16 The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work! - Provides up-to-date techniques by experts worldwide - Presents common, step-by-step protocols in an attractive, easy-to-use fashion - Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory |
| amicon ultra 0.5 ml manual: Hydrogels of Cytoskeletal Proteins Yoshihito Osada, Ryuzo Kawamura, Ken-Ichi Sano, 2016-01-22 This book describes a new family of bio-polymer gels made from cytoskeletal proteins - actin, microtubule, and tropomyosin. The importance of the gel state with multi-scale hierarchical structure is emphasized to utilize emergent functions in living organisms. Detailed protocol of gel preparation, specified method of structure investigation, and dynamic studies of self-organization, self-healing, synchronized oscillating, and autonomous motility functions are introduced together with biomimetic functions of synthetic hydrogels. |
| amicon ultra 0.5 ml manual: Structural DNA Nanotechnology Nadrian C. Seeman, 2015 Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology. |
| amicon ultra 0.5 ml manual: Structure , 2008 |
| amicon ultra 0.5 ml manual: PCR Protocols Michael A. Innis, David H. Gelfand, John J. Sninsky, Thomas J. White, 2012-12-02 The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. - Avoid contamination--with specific instructions on setting up your lab - Avoid cumbersome molecular biological techniques - Discover new applications |
| amicon ultra 0.5 ml manual: Nanocápsulas policatiónicas: nuevos sistemas para la liberación intracelular de fármacos antitumorales. , 2009 |
| amicon ultra 0.5 ml manual: Process Design Manual for Suspended Solids Removal Hazen and Sawyer, United States. Environmental Protection Agency. Office of Technology Transfer, 1975 |
| amicon ultra 0.5 ml manual: Membrane Technology and Applications Richard W. Baker, 2004-05-31 Table of Contents Preface Acknowledgments for the first edition Acknowledgments for the second edition 1 Overview of Membrane Science and Technology 1 2 Membrane Transport Theory 15 3 Membranes and Modules 89 4 Concentration Polarization 161 5 Reverse Osmosis 191 6 Ultrafiltration 237 7 Microfiltration 275 8 Gas Separation 301 9 Pervaporation 355 10 Ion Exchange Membrane Processes - Electrodialysis 393 11 Carrier Facilitated Transport 425 12 Medical Applications of Membranes 465 13 Other Membrane Processes 491 Appendix 523 Index 535. |
| amicon ultra 0.5 ml manual: The Cell Biology of Protist Parasite-Host Interfaces Carmen Faso, Adrian Hehl, 2022-04-28 |
| amicon ultra 0.5 ml manual: Pichia Protocols James M Cregg, 2007-08-08 This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition. |
| amicon ultra 0.5 ml manual: Capillary Gel Electrophoresis Andras Guttman, László Hajba, 2021-12-04 Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses |
| amicon ultra 0.5 ml manual: Handbook of Drug Monitoring Methods Amitava Dasgupta, 2007-10-23 Written in a handbook style with specific methods and tips on eliminating false positive and false negative results, this book is a practical guide to the detailed mechanisms of such occurrences. |
| amicon ultra 0.5 ml manual: Practical Handbook of Biochemistry and Molecular Biology Gerald D. Fasman, 1989-03-31 Methodologies and databases for biochemistry and molecular biology are included in this easy-to-use laboratory reference. Its logical presentation enables the reader to quickly and conveniently locate the information relevant to his or her needs. Featured are tables containing data on amino acids, proteins, nucleosides, nucleotides, and nucleic acids. Also featured are lipids and physical chemical data. Edited by a leading professional in the field, this compact, yet comprehensive bench manual serves as the definitive reference source for your laboratory. |
| amicon ultra 0.5 ml manual: Advances in Peptide and Peptidomimetic Design Inspiring Basic Science and Drug Discovery Henry I. Mosberg, Carrie Haskell-Luevano, Tomi K. Sawyer, 2020-03-13 Advances in Peptide and Peptidomimetic Design Inspiring Basic Science and Drug Discovery is a book dedicated to Prof. Victor J. Hruby on the occasion of his 80th birthday. This book includes twenty contributions from authors representing diverse multidisciplinary fields of scientific expertise, and is focused on the extraordinary potential of peptides and peptidomimetics as a surging therapeutic modality and as tools for basic research and technology development. |
| amicon ultra 0.5 ml manual: Introduction to Practical Biochemistry David T. Plummer, 2001-02 |
| amicon ultra 0.5 ml manual: Interplay Between Immunity and Fibrosis Anna-Maria Hoffmann-Vold, Oliver Distler, Jeska Kirsten De Vries-Bouwstra, 2021-12-20 Topic Editor Prof. Oliver Distler received financial support from Actelion, Bayer, Boehringer Ingelheim, Mitsubishi Tanabe Pharma companies. All other Topic Editors declare no competing interests with regards to the Research Topic subject. |
| amicon ultra 0.5 ml manual: Molecular Microbial Ecology Manual Antoon D. L. Akkermans, Jan Dirk Van Elsas, Frans J. De Bruijn, 2014-01-13 For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul tures. We now realise that only a minor fraction of the microbial com munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers. |
| amicon ultra 0.5 ml manual: Basic Exercises in Immunochemistry A. Nowotny, 2012-12-06 |
| amicon ultra 0.5 ml manual: Approaches that Foster a Pro-Regenerative Environment Carl Austin Gregory, Fei Liu, Daniel Alge, Ryang Hwa Lee, Paul Genever, 2022-03-29 |
| amicon ultra 0.5 ml manual: Dissection of the Molecular Basis of Fatty Acid Composition in Oil Crops and Molecular Breeding of Oil Crops With Improved Fatty Acid Composition Yong Xiao, Jacqueline Batley, Hyun Uk Kim, Dongdong LI, 2022-11-23 |
| amicon ultra 0.5 ml manual: Despeckle Filtering Algorithms and Software for Ultrasound Imaging Christos Loizou, Constantinos Pattichis, 2008-06-27 It is well-known that speckle is a multiplicative noise that degrades image quality and the visual evaluation in ultrasound imaging. This necessitates the need for robust despeckling techniques for both routine clinical practice and teleconsultation. The goal for this book is to introduce the theoretical background (equations), the algorithmic steps, and the MATLABTM code for the following group of despeckle filters: linear filtering, nonlinear filtering, anisotropic diffusion filtering and wavelet filtering. The book proposes a comparative evaluation framework of these despeckle filters based on texture analysis, image quality evaluation metrics, and visual evaluation by medical experts, in the assessment of cardiovascular ultrasound images recorded from the carotid artery. The results of our work presented in this book, suggest that the linear local statistics filter DsFlsmv, gave the best performance, followed by the nonlinear geometric filter DsFgf4d, and the linear homogeneous mask area filter DsFlsminsc. These filters improved the class separation between the asymptomatic and the symptomatic classes (of ultrasound images recorded from the carotid artery for the assessment of stroke) based on the statistics of the extracted texture features, gave only a marginal improvement in the classification success rate, and improved the visual assessment carried out by two medical experts. A despeckle filtering analysis and evaluation framework is proposed for selecting the most appropriate filter or filters for the images under investigation. These filters can be further developed and evaluated at a larger scale and in clinical practice in the automated image and video segmentation, texture analysis, and classification not only for medical ultrasound but for other modalities as well, such as synthetic aperture radar (SAR) images. Table of Contents: Introduction to Ultrasound Imaging / Despeckle Filtering Algorithms / Evaluation Methodology / Applications of Despeckle Filtering in Ultrasound Imaging / Comparison and Discussion of Despeckle Filtering Algorithms / Summary and Future Directions |
| amicon ultra 0.5 ml manual: Tumor Micro-environment and Drug Resistance Wei Zhao, Zhe-Sheng Chen, Liwu Fu, Yong Song, 2022-02-16 |
| amicon ultra 0.5 ml manual: The Sorghum Genome Sujay Rakshit, Yi-Hong Wang, 2017-01-30 This book provides insights into the current state of sorghum genomics. It particularly focuses on the tools and strategies employed in genome sequencing and analysis, public and private genomic resources and how all this information is leading to direct outcomes for plant breeders. The advent of affordable whole genome sequencing in combination with existing cereal functional genomics data has enabled the leveraging of the significant novel diversity available in sorghum, the genome of which was fully sequenced in 2009, providing an unmatched resource for the genetic improvement of sorghum and other grass species. Cultivated grain sorghum is a food and feed cereal crop adapted to hot and dry climates, and is a staple for 500 million of the world’s poorest people. Globally, sorghum is also an important source of animal feed and forage, an emerging biofuel crop and model for C4 grasses, particularly genetically complex sugarcane. |
| amicon ultra 0.5 ml manual: Confocal Microscopy Stephen W. Paddock, 2008-02-03 In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images. |
Michigan Unclaimed Property – Official State Site
What is Unclaimed Property? The Michigan Department of Treasury has millions of dollars worth of lost or forgotten assets from dormant bank accounts, uncashed checks, valuables left in safe …
Michigan Unclaimed Property – Official State Site
For step-by-step instructions on the claims process, please visit our How to Claim Unclaimed Property section. Claim Use this free and secure service to claim your lost funds.
Michigan Unclaimed Property – Official State Site
What is unclaimed property? Unclaimed property is cash and other items of value that have been reported to the Department of Treasury because they have gone unclaimed by the owner …
Michigan Unclaimed Property – Official State Site
Unclaimed property consists of abandoned financial assets such as checking and savings accounts, unpaid wages, securities, life insurance payouts, uncashed checks, and the contents …
Michigan Unclaimed Property – Official State Site
For each property listed, select the Claimant Relationship that best describes your relationship to the owner. After selecting the Claimant Relationship, click FILE CLAIM. Claimant …
Michigan Unclaimed Property – Official State Site
accessible only by Unclaimed Property officials, and for claims processing purposes only.
Michigan Unclaimed Property – Official State Site
How to Claim Unclaimed Property. 1. Search For Property; 2. Submit a Claim; 3. Complete Your Claim; 4. Track your Claim’s Progress
Michigan Unclaimed Property – Official State Site
accessible only by Unclaimed Property officials, and for claims processing purposes only.
Michigan Unclaimed Property – Official State Site
Claiming Property. Search for Unclaimed Properties; Check the Status of a Claim; Upload Claim Documentation; How to Claim Property; Reporting Property. Submit a Report; Reporting …
Michigan Unclaimed Property – Official State Site
Reports listed below were submitted through the Michigan Holder portal. This list includes both fully processed reports as well as recently submitted reports waiting for the transfer of funds.
Veris en calle Avenida Juan De Ascaray - Quito - Sucursales 24
Horarios Teléfonos ☎ y Toda la Info de la sucursal de Veris en la calle Avenida Juan De Ascaray de Quito.
Veris La Y - Veris Mega Y, Juan de Azcaray, y, Quito, Ecuador …
Puede contactar la empresa al (02) 600-9600. Puede encontrar más información sobre Veris La Y en veris.com.ec. Categorías: Actividades …
Veris La Y | (02) 600-9600 | Quito - TodosNegocios
Puede contactar a Veris La Y por teléfono al (02) 600-9600. Veris La Y se encuentra enVeris Mega Y, Juan de Azcaray, y, Quito, Ecuador.
Ubicaciones - Veris
Av. Juan Tanca Marengo y Av. Agustín Freire Ycaza Esq. Edificio Veris. Teléfono: 603 2 000
VERIS - Plusmedical
Mega Y: Juan de Azcaray y Mariano Jimbo, esquina. Av. 10 de agosto y Juan de Azcaray. 12 de Octubre: Av. 12 de octubre 1812 y Luis Cordero, antiguo …
