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| apoptosis analysis by flow cytometry: Flow Cytometry Protocols Teresa S. Hawley, |
| apoptosis analysis by flow cytometry: Flow Cytometry Applications in Cell Culture Mohamed Al-Rubeai, A Nichol Emery, 2020-07-24 This work present practical, biotechnological applications of flow cytometry techniques for the study of animal, plant and microbial cells, explaining methodologies for sample preparation, staining and analysis. It discusses cell variability in cell culture processes and shows how the quantitative analysis of heterogeneous populations aids in the biotechnological exploitation of cells. |
| apoptosis analysis by flow cytometry: Flow Cytometry Marion G. Macey, 2007-11-03 Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry. |
| apoptosis analysis by flow cytometry: Imaging Flow Cytometry Natasha S. Barteneva, Ivan A. Vorobjev, 2015-11-23 This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies. |
| apoptosis analysis by flow cytometry: Advanced Flow Cytometry: Applications in Biological Research R.C. Sobti, A. Krishan, 2013-04-17 Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology. |
| apoptosis analysis by flow cytometry: Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies Anna Porwit, Marie Christine Béné, 2018-01-25 Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time. |
| apoptosis analysis by flow cytometry: Apoptosis John C. Reed, 2000-07-05 Volume 322 of Methods in Enzymology is dedicated to apoptosis. Major topics covered include measuring apoptosis and apoptosis-induced endonucleases, measuring apoptosis in lower organisms, proteases involved in apoptosis and their inhibitors, cell free systems for monitoring steps in apoptosis pathways, mitochondria and apoptosis, bCl-2 family proteins, and studying receptors and signal transduction events implicated in cell survival and cell death. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. |
| apoptosis analysis by flow cytometry: Cell Proliferation and Apoptosis David Hughes, Huseyin Mehmet, 2004-03 Cell Proliferation and Apoptosis provides a detailed practical guide to cell proliferation and apoptosis detection methods. A novel approach combining both these areas allows important comparisons to be made. Topics covered include all aspects of tissue handling from collection, storage, fixation and processing through to locating and quantifying cells in different stages of the cell cycle. This book is an essential and comprehensive practical guide to these important and expanding areas. |
| apoptosis analysis by flow cytometry: Flow Cytometry and Cell Sorting Andreas Radbruch, 2013-03-14 The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended. CYTOBIOS |
| apoptosis analysis by flow cytometry: Flow Cytometry Alice Longobardi Givan, 2013-04-10 Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature. |
| apoptosis analysis by flow cytometry: Molecular Diagnostics George P. Patrinos, Wilhelm Ansorge, Phillip B. Danielson, 2016-10-27 Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. - Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories - Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression - Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field |
| apoptosis analysis by flow cytometry: Practical Flow Cytometry Howard M. Shapiro, 2005-02-25 From the reviews of the 3rd Edition... The standard reference for anyone interested in understandingflow cytometry technology. American Journal of Clinical Oncology ...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating. Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers. |
| apoptosis analysis by flow cytometry: Apoptosis and Cancer Gil Mor, Ayesha Alvero, 2014-10-13 In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research. |
| apoptosis analysis by flow cytometry: Basic Principles in Flow Cytometry Vasiliki E. Kalodimou, 2013 |
| apoptosis analysis by flow cytometry: Mitochondrial Dysfunction Caused by Drugs and Environmental Toxicants Yvonne Will, James A. Dykens, 2018-03-23 Developed as a one-stop reference source for drug safety and toxicology professionals, this book explains why mitochondrial failure is a crucial step in drug toxicity and how it can be avoided. • Covers both basic science and applied technology / methods • Allows readers to understand the basis of mitochondrial function, the preclinical assessments used, and what they reveal about drug effects • Contains both in vitro and in vivo methods for analysis, including practical screening approaches for drug discovery and development • Adds coverage about mitochondrial toxicity underlying organ injury, clinical reports on drug classes, and discussion of environmental toxicants affecting mitochondria |
| apoptosis analysis by flow cytometry: Flow Cytometry Zbigniew Darzynkiewicz, J. Paul Robinson, 1994 Flow Cytometry, Second Edition is a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Unique comprehensive methodological coverage * In-depth treatment of procedures, including: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who developed or modified the techniques * Methods cover cell death and cell cycle analyses * Practical, handbook-style presentation works in lab and in the classroom * Color plates illustrate technique |
| apoptosis analysis by flow cytometry: Introduction to Flow Cytometry James V. Watson, 1991-04-26 Flow cytometry allows a cell population to be analysed. This book describes the technology involved. |
| apoptosis analysis by flow cytometry: Clinical Flow Cytometry Ingrid Schmid, 2012-05-16 Clinical Flow Cytometry - Emerging Applications contains a collection of reviews and original papers that illustrate the relevance of flow cytometry for the study of specific diseases and clinical evaluations. The chapters have been contributed by authors from a wide variety of countries showing the broad application and importance of this technology in medicine. Examples include chapters on autoimmune disease, cancer, and the evaluation of new drugs. The book is intended to give newcomers a helpful introduction, but also to provide experienced flow cytometrists with novel insights and a better understanding of clinical cytometry. |
| apoptosis analysis by flow cytometry: The Microflow Cytometer Frances S. Ligler, Jason S. Kim, 2010-05-31 Great book! Excellent compilation. From history of the very early days of flow cytometers to the latest unique unconventional microflow cytometers. From commercialization philosophy to cutting edge engineering designs. From fluid mechanics to optics to electronic circuit considerations. Well balanced and comprehensive.--Shuichi Takayama University of Michigan, USA. |
| apoptosis analysis by flow cytometry: Cytoskeleton Jose C. Jimenez-Lopez, 2017-05-17 The cytoskeleton is a highly dynamic intracellular platform constituted by a three-dimensional network of proteins responsible for key cellular roles as structure and shape, cell growth and development, and offering to the cell with motility that being the ability of the entire cell to move and for material to be moved within the cell in a regulated fashion (vesicle trafficking). The present edition of Cytoskeleton provides new insights into the structure-functional features, dynamics, and cytoskeleton's relationship to diseases. The authors' contribution in this book will be of substantial importance to a wide audience such as clinicians, researches, educators, and students interested in getting updated knowledge about molecular basis of cytoskeleton, such as regulation of cell vital processes by actin-binding proteins as cell morphogenesis, motility, their implications in cell signaling, as well as strategies for clinical trial and alternative therapies based in multitargeting molecules to tackle diseases, that is, cancer. |
| apoptosis analysis by flow cytometry: Flow Cytometry M. G. Ormerod, David Novo, 2008 Flow cytometry is a technique used to study cells, such as blood cells or cancer cells. It is used in medical and research laboratories. |
| apoptosis analysis by flow cytometry: Flow Cytometry M. G. Ormerod, 1990 The recent explosion in the field of cytometry and the rapid increase in the number of machines being installed has resulted in many comparatively inexperienced workers trying to cope with this new technology. Stressing clinical applications, this book offers practical guidance in preparing and analysing samples. Easily accessible to those new to this field, the volume will also act as a first point of reference for established workers who wish to add to their repertoire of applications. |
| apoptosis analysis by flow cytometry: General Methods in Biomarker Research and their Applications Victor R. Preedy, Vinood B. Patel, 2015-08-14 In the past decade there has been a major sea change in the way disease is diagnosed and investigated due to the advent of high throughput technologies, such as microarrays, lab on a chip, proteomics, genomics, lipomics, metabolomics etc. These advances have enabled the discovery of new and novel markers of disease relating to autoimmune disorders, cancers, endocrine diseases, genetic disorders, sensory damage, intestinal diseases etc. In many instances these developments have gone hand in hand with the discovery of biomarkers elucidated via traditional or conventional methods, such as histopathology or clinical biochemistry. Together with microprocessor-based data analysis, advanced statistics and bioinformatics these markers have been used to identify individuals with active disease or pathology as well as those who are refractory or have distinguishing pathologies. New analytical methods that have been used to identify markers of disease and is suggested that there may be as many as 40 different platforms. Unfortunately techniques and methods have not been readily transferable to other disease states and sometimes diagnosis still relies on single analytes rather than a cohort of markers. There is thus a demand for a comprehensive and focused evidenced-based text and scientific literature that addresses these issues. Hence the formulation of Biomarkers in Disease. The series covers a wide number of areas including for example, nutrition, cancer, endocrinology, cardiology, addictions, immunology, birth defects, genetics and so on. The chapters are written by national or international experts and specialists. |
| apoptosis analysis by flow cytometry: Cytotoxicity Erman Salih Istifli, Hasan Basri İla, 2019-10-02 Compensating for cytotoxicity in the multicellular organism by a certain level of cellular proliferation is the primary aim of homeostasis. In addition, the loss of cellular proliferation control (tumorigenesis) is at least as important as cytotoxicity, however, it is a contrasting trauma. With the disruption of the delicate balance between cytotoxicity and proliferation, confrontation with cancer can inevitably occur. This book presents important information pertaining to the molecular control of the mechanisms of cytotoxicity and cellular proliferation as they relate to cancer. It is designed for students and researchers studying cytotoxicity and its control. |
| apoptosis analysis by flow cytometry: Hepatocytes Patrick Maurel, 2010-05-07 Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular BiologyTM series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy. |
| apoptosis analysis by flow cytometry: Cytometry, Part A , 2000-10-31 Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area. - Contains 56 extensive chapters authored by world authorities on cytometry - Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases - Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine - Complements the first and second editions on flow cytometry in the Methods in Cell Biology series and includes new sections on technology principles |
| apoptosis analysis by flow cytometry: Cellular Electron Microscopy J. Richard McIntosh, 2011-09-02 Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. - Updates the numerous technological innovations that have improved the capabilities of electron microscopy - Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging - Chapters include a balance of how to, so what and where next, providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going |
| apoptosis analysis by flow cytometry: Pathophysiology of Shock, Anoxia, and Ischemia R Adams Cowley, Benjamin F. Trump, 1982 |
| apoptosis analysis by flow cytometry: Manual of Sperm Function Testing in Human Assisted Reproduction Ashok Agarwal, Ralf Henkel, Ahmad Majzoub, 2021-04-15 Focusing on modern sperm function testing, this guide is essential in selecting sperm that will produce viable and healthy embryos. |
| apoptosis analysis by flow cytometry: Organic Materials as Smart Nanocarriers for Drug Delivery Alexandru Mihai Grumezescu, 2018-03-26 Organic Materials as Smart Nanocarriers for Drug Delivery presents the latest developments in the area of organic frameworks used in pharmaceutical nanotechnology. An up-to-date overview of organic smart nanocarriers is explored, along with the different types of nanocarriers, including polymeric micelles, cyclodextrins, hydrogels, lipid nanoparticles and nanoemlusions. Written by a diverse range of international academics, this book is a valuable reference for researchers in biomaterials, the pharmaceutical industry, and those who want to learn more about the current applications of organic smart nanocarriers. - Explores the most recent molecular- and structure-based applications of organic smart nanocarriers in drug delivery - Highlights different smart nanocarriers and assesses their intricate organic structural properties for improving drug delivery - Assesses how molecular organic frameworks lead to more effective drug delivery systems |
| apoptosis analysis by flow cytometry: Practical Handbook of Cell Therapy Cryopreservation Michael H. Creer, M. Victor Lemas, Aby J. Mathew, 2015-10 |
| apoptosis analysis by flow cytometry: Pharmaceutical Applications of Dendrimers Abhay Singh Chauhan, Hitesh Kulhari, 2019-11-15 Pharmaceutical Applications of Dendrimers explores the applications of dendrimers in the solubilization of hydrophobic active ingredients, drug delivery, gene delivery, imaging, diagnosis and photodynamic therapy. The book discusses the diagnostic applications of dendrimers, including their use as MRI contrast agents and in the imaging of diseased areas. In addition, the anti-inflammatory, antimicrobial and antiviral properties of PPI and PAMAM are also covered, along with a discussion on photosensitizers, such as rose Bengal and protoporphyrin IX that have been delivered using PAMAM and PPI dendrimers for the treatment of cancer. This book is an important research reference for those who want to learn more about the development of dendrimer-based solutions for drug delivery. |
| apoptosis analysis by flow cytometry: When Cells Die Richard A. Lockshin, 1998-04-13 When Cells Die A Comprehensive Evaluation of Apoptosis and Programmed Cell Death Edited by Richard A. Lockshin, Zahra Zakeri, and Jonathan L. Tilly Cell death is fast becoming one of the most dynamic areas of biological research -involving as it does the study of apoptosis and programmed cell death and the role these phenomena play in development and homeostasis on the one hand, and aging and disease on the other. The profound implications for medicine and agriculture from the manipulation of these processes have spawned a deluge of research papers, articles, approaches, and methods -making it difficult for scientists to get an overview of the field. When Cells Die establishes a coherent framework for the study of cell death -cutting across viewpoints and disciplines and consolidating disparate research efforts. Leading international researchers describe a wide range of topics, including evaluation methods for programmed cell death and apoptosis in numerous tissues and circumstances; genetic mechanism, signal transduction, and observed manifestations of physiological cell death; model systems ranging from nematodes to humans; relevant work in cancer research, AIDS, immune disorders, fertility, eye disease, and Alzheimer's disease; and more. Written to provide an in-depth overview of cell death, the book is divided into five major parts: * The phenomenon of cell death * Themes and approaches to cell death * Cell death where mitosis is high and evanescence is desirable * Cell death in long-lived cells * The clinical relevance of apoptosis. When Cells Die offers a comprehensive introduction to an intriguing discipline, insight into areas in need of exploration, and information on new techniques and therapeutic applications -all supported with diagrams and flowcharts and a fully cross-referenced and indexed text. It is important reading for anyone working in cell and developmental biology, neuroscience, immunology, cancer research, and virology. It is also useful for advanced undergraduate and graduate-level students, postdoctoral fellows, and researchers just entering the field. |
| apoptosis analysis by flow cytometry: Eosinophils Garry M. Walsh, 2016-08-23 In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell. |
| apoptosis analysis by flow cytometry: Checkpoint Controls and Cancer Axel H. Schönthal, 2004-06-24 Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors. Ext- nal stresses can be generated by the continuous assault of DNA-damaging agents, such as environmental mutagens, ultraviolet (UV) light, ionizing radiation, or the reactive oxygen species that can arise during normal cellular metabolism. In response to any of these assaults on the integrity of the genome, the activation of the network of checkpoint control pathways can lead to diverse cellular responses, such as cell cycle arrest, DNA repair, or elimination of the cell by cell death (apoptosis) if the damage cannot be repaired. Moreover, internal errors can occur during the highly orchestrated replication of the cellular genome and its distribution into daughter cells. Here, the temporal order of these cell cycle events must be strictly enforced—for example, to ensure that DNA replication is c- plete and occurs only once before cell division, or to monitor mitotic spindle assembly, and to prevent exit from mitosis until chromosome segregation has been completed. Thus, well functioning checkpoint mechanisms are central to the maintenance of genomic integrity and the basic viability of cells and, the- fore, are essential for proper development and survival. The importance of proper functioning of checkpoints becomes plainly obvious under conditions in which this control network malfunctions and fails. Depending on the severity and timing, failure of this machinery can lead to embryonic lethality, genetic diseases, and cancer. |
| apoptosis analysis by flow cytometry: Nutrition and Immunology D.M. Klurfeld, 2013-08-01 Historically, nutrient deficiencies have been of greater concern than dietary excess. However, along with the realization that deaths due to certain diseases are more prevalent in affluent countries came the conclusion that nutritional excess is of equal or in greater concern in many nations. Because immunologic reactions may play a role development of both cancer and atherosclerosis, better understanding of these interre lated phenomena may lead to innovative ideas for control of these diseases. There has been considerable interest in the role various nutrients may play in regulating immunologic responses. This has been especially true as a possible mecha nism by which fat modulates growth of tumors in animals. Likewise, deficiency or excess of a number of other individual nutrients have been linked to altered immune responses. This volume of Human Nutrition-A Comprehensive Treatise details the effects of a number of nutrients on immunity. The first chapter covers questionable and fraudu lent claims linking nutrition and immunity. The next chapter examines several aspects of food allergy. Ensuing chapters focus on specific nutrients such as fat, cholesterol, arginine, vitamins C, A, and E, carotenoids, flavonoids, zinc, iron, copper, and sele nium. There are two chapters on total energy intake affecting immune response, one examining protein-energy malnutrition and the other describing the effects of food restriction in otherwise healthy animals. |
| apoptosis analysis by flow cytometry: Apoptosis in Toxicology Ruth A. Roberts, 1999 The first book to focus on the molecular regulation of apoptosis with particular emphasis on toxicant action. A timely volume written by leading authorities in their respective fields. Will have broad appeal. |
| apoptosis analysis by flow cytometry: Mechanisms of Cell Death Zahra Zakeri, Richard A. Lockshin, Luis Benitez-Bribiesca, 1999 Contains papers from a July 1998 conference held at the Queens College Campus of the City University of New York. Papers are arranged in sections on mechanisms and general considerations, programmed (developmental) cell death, and cell death and pathological and clinical situations. Specific topics |
| apoptosis analysis by flow cytometry: Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays Marica Gemei, 2018 Flow cytometry's informative potential has been underestimated for many years because of a lack of adequate instruments, automation, reagents, and know-how to approach, integrate, and also substitute other techniques giving single information per assay. In the last decade, flow cytometers have become capable of performing high-throughput screening and high content analysis, evaluating tens of different samples' features in a single run up to 1536 formats on multiple cell populations. The introduction of imaging flow cytometry has filled the gap between flow cytometry and conventional high content imaging screening, putting flow cytometry at the center of many laboratories, which can now cover with a single instrument the vast majority of needs in research programs. The flow cytometry community is a multidisciplinary and diversified group with many different interests and fields of action. These characteristics have prompted the evolution of the techniques, applications, and instruments that allow the use of complex, sophisticated, and standardized and reliable flow cytometric assays in academic and industrial programs. |
| apoptosis analysis by flow cytometry: Flow Cytometry, Part A , 1994-12-12 Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.Key Features* Completely revised and greatly expanded since the publication of the First Edition in 1990* Methods cover cell death and cell cycle analysesPractical, handbook-style presentation works in lab or classroom* Unique comprehensive methodological coverage* Color plates illustrate techniques* In-depth treatment of procedures, including a description of each procedure:* Theoretical foundations* Critical aspects* Possible pitfalls* Written by authors with extensive experience who:* Developed or modified the techniques* Describe their experience with different instruments and applications to different cell systems* Are the Who's Who in Flow Cytometry |
Measuring Apoptosis using Annexin V and Flow Cytometry
Apoptosis Perforin/Granzyme (T-cell mediated) From: Janeway’s Immunology, Seventh Edition, 2008. Healthy Cell. Apoptotic Cell. Top panels show a cytotoxic T-cell inducing apoptosis in a …
Detection of Apoptosis Using the BD Annexin V FITC Assay on …
Annexin V, a Ca2+-dependent phospholipid-binding protein, has high affinity for PS, and fluorochrome-labeled Annexin V can be used for the detection of exposed PS using flow …
Protocol for Apoptosis Assay by Flow Cytometry Using …
[Abstract] This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium Iodide …
Apoptosis Detection by Flow Cytometry November 2020
The study of the cell changes during apoptosis can be done with flow cytometry. A unique feature of apoptosis is the externalization of phosphatidylserine (PS) in the phospholipid bilayer. …
Flow cytometry analysis of dose response for apoptosis …
Data on cellular apoptosis via caspase-3/7 activity were acquired on an Attune NxT Flow Cytometer (4-laser) with Autosampler with the Invitrogen ™ Attune NxT Software
Quantitative Measurements of Apoptosis using the NovoCyte …
To demonstrate the flexibility and versatility of the Agilent NovoCyte flow cytometer system in assessing apoptosis, three assays were carried out to measure apoptosis in response to …
Chapter 10 Multiparametric Analysis of Apoptosis by Flow …
This chapter provides methods for combining assays for single apoptotic characteristics like caspase activation, annexin V binding, and cell membrane perme-ability into multiparametric …
Flow Cytometry of Apoptosis UNIT 18 - biotech.illinois.edu
This unit describes the most common methods applicable to flow cytometry that make it possible to: (1) identify and quantify dead or dying cells, (2) reveal a mode of cell death (apoptosis or …
Analysis of apoptosis by propidium iodide staining and flow …
Nov 9, 2006 · content by flow cytometric analysis, and subsequent identification of hypodiploid cells. The original protocol enhanced the capacity for a rapid, quantitative measure of cell …
Flow cytometry-based quantitative analysis of cellular protein ...
Flow cytometry techniques utilizing dual staining with annexin V and propidium iodide (PI) provide a robust method for quantitatively analyzing apoptosis induction. Annexin V binds …
Flow Cytometry Method Analysis of Apoptosis - SAGE Journals
Apoptosis plays a major role in various physiological and pathological processes, which can be analyzed by flow cytometry method (FCM), a generally accepted and reliable tool for detection …
Apoptosis Analysis Using the Cytek Northern Lights Flow …
Flow cytometry is a powerful and well-established technique to study apoptosis, allowing statistically significant data to be rapidly obtained on a large number of cells and offers clear …
Apoptosis Detection Using the BD Accuri™ C6 Flow Cytometer
available to measure apoptosis. • Apoptosis detection by flow cytometry can be done easily, using two colors. • The BD Accuri C6 is a perfect analysis tool for new and experienced users.
Analysis of Apoptosis by Flow Cytometry - Springer
Analysis of Apoptosis by Flow Cytometry Wojciech Gorczyca, Myron R. Melamed, and Zbigniew Darzynkiewicz 1. Introduction Based on morphological, biochemical, and molecular criteria two …
Cell Death Analysis in Flow Cytometry - Aarhus Universitet
Apoptosis, things to bear in mind before you start: • Apoptosis is a very variable process depending of cell type. Always use more than one method for detecting apoptosis. • A good …
Using flow cytometry to follow the apoptotic cascade
Flow cytometry has been extensively used to follow the apoptotic cascade and to enumerate apoptotic cells, both in cell cultures and, to a lesser extent, in tissue biopsies. An overview of …
Analysis of apoptosis by propidium iodide staining and flow …
Nov 9, 2006 · In 1991, we published a rapid and simple flow cytometry method for measuring apoptosis in propidium iodide (PI)-stained mouse thymocytes6. With slight changes to the …
A learning for imaging flow cytometry - BD Biosciences
Using a cellular apoptotic model, we captured label-free images in a heterogeneous sample using high-throughput imaging flow cytometry. A novel deep learning algorithm was applied to …
Chapter 15 Flow Cytometric Analysis of Regulated Cell Death
Flow cytometry assays use primary antibodies that bind to specific antigens (or markers) to detect apoptosis. The primary markers of apoptosis include caspase activation, degradation of …
Multiparameter Analysis of Apoptosis Using Lab-on-a-Chip …
integrated microfluidic flow cytometry system, Fishman-R. The first protocol describes the use of a new plasma membrane–permeability marker, DRAQ7, and the fluorogenic caspase …
Measuring Apoptosis using Annexin V and Flow Cytom…
Apoptosis Perforin/Granzyme (T-cell mediated) From: Janeway’s Immunology, Seventh Edition, 2008. …
Detection of Apoptosis Using the BD Annexin V FI…
Annexin V, a Ca2+-dependent phospholipid-binding protein, has high affinity for PS, and fluorochrome …
Protocol for Apoptosis Assay by Flow Cytometry Using A…
[Abstract] This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will …
Apoptosis Detection by Flow Cytometry November 202…
The study of the cell changes during apoptosis can be done with flow cytometry. A unique feature of …
Flow cytometry analysis of dose response for apoptos…
Data on cellular apoptosis via caspase-3/7 activity were acquired on an Attune NxT Flow Cytometer (4-laser) with …
