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Decoding the Agilent 6890: A Comprehensive Troubleshooting Guide
Author: Dr. Evelyn Reed, PhD, Analytical Chemistry; 15+ years experience in GC maintenance and troubleshooting within pharmaceutical and environmental testing labs.
Publisher: Chromatography Solutions Inc. – A leading provider of chromatography instrument support and training materials with over 20 years of industry experience.
Editor: Mark Johnson, MSc Analytical Chemistry; 10+ years editing scientific publications and technical manuals focusing on analytical instrumentation.
Summary: This comprehensive guide delves into the intricacies of Agilent 6890 Gas Chromatograph troubleshooting, addressing common issues, preventative maintenance, and the broader implications of efficient GC operation within various industries. It provides practical solutions and expert insights for maximizing instrument uptime and data integrity.
Keywords: Agilent 6890 troubleshooting guide, Agilent 6890 GC troubleshooting, Gas chromatography troubleshooting, GC maintenance, Agilent 6890 repair, HPLC troubleshooting, Analytical instrumentation, Chromatography troubleshooting
1. Introduction: Mastering the Agilent 6890
The Agilent 6890 Gas Chromatograph (GC) remains a cornerstone instrument in numerous analytical laboratories worldwide. Its versatility and reliability are critical for a vast range of applications, from environmental monitoring and food safety analysis to pharmaceutical quality control and petrochemical testing. However, even the most robust instrument requires occasional maintenance and troubleshooting. An effective Agilent 6890 troubleshooting guide is therefore essential for maintaining high levels of productivity and data quality. This guide aims to provide a comprehensive resource for technicians and scientists working with the Agilent 6890, empowering them to diagnose and resolve common problems efficiently.
2. Common Agilent 6890 Problems and Solutions
This section of our Agilent 6890 troubleshooting guide focuses on the most frequently encountered issues. We'll explore practical solutions supported by real-world examples.
Poor Peak Shape: This can stem from various causes, including column issues (bleeding, contamination, improper installation), injector problems (split ratio, septum leaks), and detector malfunctions (contamination, low sensitivity). Our guide offers a systematic approach to identifying the root cause, covering checks for column integrity, injector cleaning, and detector optimization.
Ghost Peaks: These unwanted peaks can arise from column contamination, sample carryover, or system leaks. The Agilent 6890 troubleshooting guide explains how to implement effective cleaning procedures for both the injector and detector, along with methods for identifying and rectifying leaks in the system.
Baseline Drift: Baseline instability often indicates detector issues or problems with the carrier gas flow. The guide details how to troubleshoot these problems, checking for gas leaks, detector contamination, and appropriate temperature control.
Loss of Sensitivity: Reduced detector response can be attributed to several factors, including detector contamination, column degradation, or insufficient carrier gas flow. Our Agilent 6890 troubleshooting guide provides a stepwise process for investigating these aspects and restoring optimal sensitivity.
Injector Problems: Septum leaks, improper split ratio settings, and injector liner contamination are frequent sources of difficulty. This guide includes detailed instructions for septum replacement, split ratio optimization, and liner cleaning or replacement.
Carrier Gas Issues: Problems with the carrier gas supply, such as pressure fluctuations or contamination, can significantly impact performance. The guide explains how to diagnose and address these issues, including checking for gas leaks and ensuring the purity of the carrier gas.
3. Preventative Maintenance: Extending the Lifespan of Your Agilent 6890
Preventative maintenance is crucial for minimizing downtime and maximizing the lifespan of your Agilent 6890. A well-structured Agilent 6890 troubleshooting guide should incorporate a robust preventative maintenance schedule. This involves regular checks of:
Carrier gas flow rates and pressures: Consistent monitoring prevents issues arising from gas leaks or supply problems.
Injector and detector cleanliness: Regular cleaning and maintenance of these crucial components are crucial.
Column condition: Checking for column degradation helps identify potential issues before they significantly impact analysis.
Septum integrity: Replacing the septum at regular intervals prevents leaks and improves performance.
Instrument temperature control: Ensuring proper temperature control for the oven, injector, and detector is critical for consistent and reliable results.
4. The Impact of Efficient GC Operation
The efficient operation of the Agilent 6890, aided by a comprehensive Agilent 6890 troubleshooting guide, has significant implications across diverse industries. Reliable analysis ensures:
Improved Data Quality: Accurate and precise data forms the foundation of informed decision-making in various applications.
Increased Productivity: Minimizing downtime through effective troubleshooting maximizes laboratory efficiency.
Reduced Costs: Preventative maintenance and timely repairs prevent costly instrument failures.
Enhanced Regulatory Compliance: Accurate results are crucial for meeting regulatory requirements in industries like pharmaceuticals and environmental monitoring.
5. Conclusion
The Agilent 6890 troubleshooting guide presented here serves as a valuable tool for maximizing the efficiency and reliability of this essential analytical instrument. By addressing common issues, promoting preventative maintenance, and understanding the broader implications of efficient GC operation, laboratories can ensure the production of high-quality data, enhance productivity, and contribute to advancements across various scientific fields.
FAQs
1. How often should I replace the Agilent 6890 septum? Generally, replace it every 10-20 injections, or more frequently if you observe leaks or poor peak shapes.
2. What causes ghost peaks in Agilent 6890 GC? Ghost peaks are typically due to column contamination, sample carryover, or system leaks.
3. How can I improve the baseline stability of my Agilent 6890? Check for gas leaks, detector contamination, and ensure appropriate temperature control.
4. My Agilent 6890 has lost sensitivity. What are the possible causes? Consider detector contamination, column degradation, or insufficient carrier gas flow.
5. What are the common injector problems encountered with the Agilent 6890? Septum leaks, improper split ratio settings, and injector liner contamination are common.
6. How do I check for leaks in my Agilent 6890 GC system? Use a leak detector, check connections visually, and monitor pressure fluctuations.
7. What is the importance of preventative maintenance for my Agilent 6890? Preventative maintenance extends instrument lifespan, minimizes downtime, and improves data quality.
8. How does an effective Agilent 6890 troubleshooting guide impact laboratory productivity? It minimizes downtime and ensures faster problem resolution.
9. What industries benefit most from a comprehensive Agilent 6890 troubleshooting guide? Pharmaceuticals, environmental monitoring, food safety, and petrochemical industries are among the primary beneficiaries.
Related Articles:
1. Agilent 6890 GC Injector Maintenance and Repair: A detailed guide focusing on injector troubleshooting and maintenance procedures.
2. Troubleshooting Agilent 6890 GC Detector Problems: Concentrates specifically on diagnosing and fixing common detector issues.
3. Agilent 6890 GC Column Selection and Optimization: Covers the importance of selecting and optimizing columns for optimal separation.
4. Understanding Agilent 6890 GC Carrier Gas Systems: Explores the critical role of carrier gas in GC analysis.
5. Preventative Maintenance for Agilent 6890 GC: A comprehensive schedule for regular maintenance to avoid potential issues.
6. Agilent 6890 GC Method Development and Validation: Guide to method development and validation for various applications.
7. Data Integrity in Agilent 6890 GC Analysis: Focuses on maintaining data integrity in GC analysis.
8. Common Errors and their Solutions in Agilent 6890 GC: Covers various common errors and their efficient solutions.
9. Comparison of Agilent 6890 GC with Other Models: Compares features, performance, and applications of Agilent 6890 GC with other GC models.
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| agilent 6890 troubleshooting guide: Split and Splitless Injection for Quantitative Gas Chromatography Konrad Grob, 2008-11-21 This comprehensive and unique handbook of split and splitless injection techniques has been completely revised and updated. This new edition offers: - New insights concerning sample evaporation in the injector - Information about matrix effects - A new chapter on injector design The real processes within the injector are for the first time visualized and explained by the CD-ROM included in the book. Furthermore the reader will understand the concepts of injection techniques and get a knowledge of the sources of error. The handbook also includes many practical guidelines. From reviews of former editions: This substantial book is on injection techniques alone, which ... demonstrates this can have many pitfalls ... no one should be allowed to direct a laboratory doing quantitative analysis by GC without first being thoroughly familar with this book ... The Analyst This is a detailed reference volume filled with practical suggestions and techniques for managing split and splitless injection in the day-to-day world of the working gas chromatographer. It will be useful ... for anyone who must work hands-on with GC. Journal of High Resolution Chromatography |
| agilent 6890 troubleshooting guide: Amino Acid Analysis Michail A. Alterman, Peter Hunziker, 2011-12-02 Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences. |
| agilent 6890 troubleshooting guide: Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry Rainer Cramer, 2015-11-09 This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics |
| agilent 6890 troubleshooting guide: Drugs and Poisons in Humans Osamu Suzuki, Kanako Watanabe, 2006-01-16 Unique analysis of drugs and poisons to facilitate testing in all laboratories even by inexperienced chemists Includes source of chemicals needed for the experiments Texts are composed by 67 experts in analyzing the respective compounds Clear and uniform structure of chapters for ease of reading The text is illustrated by many diagrams and tables |
| agilent 6890 troubleshooting guide: Practical HPLC Method Development Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch, 2012-12-03 This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations. |
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| agilent 6890 troubleshooting guide: Environmental Sustainability and Education for Waste Management Winnie Wing Mui So, Cheuk Fai Chow, John Chi Kin Lee, 2019-07-25 This book focuses on education for environmental sustainability, in particular the area of solid waste management. Presenting the latest studies from different countries, industries and education sectors on the approaches and innovative ideas to educate future citizens regarding sustainable development of our planet, it is of interest to educators, academics, tertiary students, policy-makers, environmental scientists, social scientists and practitioners who have been involved in education, policy, science, and technological innovation for solid waste management. |
| agilent 6890 troubleshooting guide: Selective Detectors Robert E. Sievers, 1995-04-03 A timely and authoritative review of the current state of selective detector technology This book was written for professionals who need to keep abreast of the latest developments and emerging trends in selective detectors and their applications. It comprises contributions from many of the leading innovators and pioneers in the field, including James Lovelock, inventor of the electron capture detector, whose own contribution is certain to be a rich source of ideas and inspiration for all who read it. Offering a balanced presentation of theory and practice, Selective Detectors: Reviews the theory and underlying principles of a broad range of devices Discusses, in detail, capabilities and current applications, with an emphasis on interdisciplinary applications, including environmental, petrochemical, biomedical, and quality control Explores, in depth, the latest advances and emerging technologies Arms readers with a wealth of practical how-to information on selecting, using, modifying, and building selective detectors for a wide range of applications Future historians studying the late twentieth century will almost certainly come to view the advent of selective detectors as among the truly formative technological developments of the period. Anyone who doubts this thesis need only consider the impact of selective detection on environmental quality, the sciences, technology, medicine, business and industry, public policy, quality control, and many other fields. Yet, despite the obvious importance of selective detectors, there continues to be a scarcity of books dedicated to helping professionals keep abreast of the latest developments and emerging trends in this in fluential technology. This timely and authoritative review of the current state of selective detector technology fills that gap. This book focuses on the newest selective detectors for chromatographic analysis. Conceived and shepherded into existence by a major figure in analytical chemistry and environmental analysis, it includes contributions from many of the leading innovators and pioneers in the field. Most prominent among these is Dr. James Lovelock, inventor of the electron capture detector, whose chapter on the history and development of selective detectors will be a rich source of ideas and inspiration for all who read it. Offering a balanced presentation of theory and practice, Selective Detectors reviews the theory and underlying principles of selective detectors; discusses, in detail, their current capabilities and applications; explores the latest advances and emerging technologies; and arms readers with a wealth of practical how-to information on selecting, using, modifying, and building selective detectors for a wide range of applications. Selective Detectors is an invaluable resource for analytical chemists and technicians working in a variety of disciplines, including environmental science, petrochemical industries, the food and beverage industries, biotechnology, medicine, and more. |
| agilent 6890 troubleshooting guide: Vietnam Studies the War in the Northen Provinces 1966-1968 Army Center for MIlitary History, 2013-12-16 An account of North Vietnamese attempts to seize control of Quang Tri and Thua Thien Provinces and the response of the allied forces, particularly U.S. Army units. Contents Chapter I. EARLY DEVELOPMENTS Background The Northern Border, 1965-1967 Continuing Activity Along the Demilitarized Zone II. PREPARING FOR A SHOWDOWN The Anti-Infiltration System Free World Forces The Growth of Logistic Facilities Upgrading of the Vietnamese Army Forces III. THE BLEAK PICTURE Operation Niagara. The Battle of Keh Sanh- Opening Round The Tet Offensive--First Phase The Battle for Hue Intelligence Battle for Quang Tri Enemy Attacks on the Logistical System Task Force Clearwater IV. U.S. RESPONSE TO THE TET OFFENSIVE Planning for the Reliel of Khe Sanh Single Manager for Air Concept V. KHE SANH AND PEGASUS Planning for Pegasus Operation Orders VI. THE FREE WORLD COUNTEROFFENSIVE Opening Operations Back to A Shau VII. ANALYSIS OF NORTH VIETNAMES GOALS AND FAILURES Intelligence Organization for Combat Airmobility Superior Firepower Communications Logistics Improvement of Vietnamese Armed Forces The Other War Conclusion GLOSSARY INDEX |
| agilent 6890 troubleshooting guide: Natural Products Isolation Satya D. Sarker, 2008-02-05 The term “natural products” spans an extremely large and diverse range of chemical compounds derived and isolated from biological sources. Our interest in natural products can be traced back thousands of years for their usefulness to humankind, and this continues to the present day. Compounds and extracts derived from the biosphere have found uses in medicine, agriculture, cosmetics, and food in ancient and modern societies around the world. Therefore, the ability to access natural products, understand their usefulness, and derive applications has been a major driving force in the field of natural product research. The first edition of Natural Products Isolation provided readers for the first time with some practical guidance in the process of extraction and isolation of natural products and was the result of Richard Cannell’s unique vision and tireless efforts. Unfortunately, Richard Cannell died in 1999 soon after completing the first edition. We are indebted to him and hope this new edition pays adequate tribute to his excellent work. The first edition laid down the “ground rules” and established the techniques available at the time. Since its publication in 1998, there have been significant developments in some areas in natural product isolation. To capture these developments, publication of a second edition is long overdue, and we believe it brings the work up to date while still covering many basic techniques known to save time and effort, and capable of results equivalent to those from more recent and expensive techniques. |
| agilent 6890 troubleshooting guide: Static Headspace-Gas Chromatography Bruno Kolb, Leslie S. Ettre, 2006-04-20 STATIC HEADSPACE-GAS CHROMATOGRAPHY THE ONLY REFERENCE TO PROVIDE BOTH CURRENT AND THOROUGH COVERAGE OF THIS IMPORTANT ANALYTICAL TECHNIQUE Static headspace-gas chromatography (HS-GC) is an indispensable technique for analyzing volatile organic compounds, enabling the analyst to assay a variety of sample matrices while avoiding the costly and time-consuming preparation involved with traditional GC. Static Headspace-Gas Chromatography: Theory and Practice has long been the only reference to provide in-depth coverage of this method of analysis. The Second Edition has been thoroughly updated to reflect the most recent developments and practices, and also includes coverage of solid-phase microextraction (SPME) and the purge-and-trap technique. Chapters cover: Principles of static and dynamic headspace analysis, including the evolution of HS-GC methods and regulatory methods using static HS-GC Basic theory of headspace analysis—physicochemical relationships, sensitivity, and the principles of multiple headspace extraction HS-GC techniques—vials, cleaning, caps, sample volume, enrichment, and cryogenic techniques Sample handling Cryogenic HS-GC Method development in HS-GC Nonequilibrium static headspace analysis Determination of physicochemical functions such as vapor pressures, activity coefficients, and more Comprehensive and focused, Static Headspace-Gas Chromatography, Second Edition provides an excellent resource to help the reader achieve optimal chromatographic results. Practical examples with original data help readers to master determinations in a wide variety of areas, such as forensic, environmental, pharmaceutical, and industrial applications. |
| agilent 6890 troubleshooting guide: Basic Gas Chromatography Harold M. McNair, James M. Miller, 2011-09-20 The New Edition of the Well-Regarded Handbook on Gas Chromatography Since the publication of the highly successful first edition of Basic Gas Chromatography, the practice of chromatography has undergone several notable developments. Basic Gas Chromatography, Second Edition covers the latest in the field, giving readers the most up-to-date guide available, while maintaining the first edition's practical, applied approach to the subject and its accessibility to a wide range of readers. The text provides comprehensive coverage of basic topics in the field, such as stationary phases, packed columns and inlets, capillary columns and inlets, detectors, and qualitative and quantitative analysis. At the same time, the coverage also features key additions and updated topics including: Gas chromatography-mass spectrometry (GC-MS) Sampling methods Multidimensional gas chromatography Fast gas chromatography Gas chromatography analysis of nonvolatile compounds Inverse gas chromatography and pyrolysis gas chromatography Along with these new and updated topics, the references, resources, and Web sites in Basic Gas Chromatography have been revised to reflect the state of the field. Concise and fundamental in its coverage, Basic Gas Chromatography, Second Edition remains the standard handbook for everyone from undergraduates studying analytical chemistry to working industrial chemists. |
| agilent 6890 troubleshooting guide: Modern Practice of Gas Chromatography Robert L. Grob, PhD, Eugene F. Barry, 2004-08-04 The bible of gas chromatography-offering everything the professional and the novice need to know about running, maintaining, and interpreting the results from GC Analytical chemists, technicians, and scientists in allied disciplines have come to regard Modern Practice of Gas Chomatography as the standard reference in gas chromatography. In addition to serving as an invaluable reference for the experienced practitioner, this bestselling work provides the beginner with a solid understanding of gas chromatographic theory and basic techniques. This new Fourth Edition incorporates the most recent developments in the field, including entirely new chapters on gas chromatography/mass spectrometry (GC/MS); optimization of separations and computer assistance; high speed or fast gas chromatography; mobile phase requirements: gas system requirements and sample preparation techniques; qualitative and quantitative analysis by GC; updated information on detectors; validation and QA/QC of chromatographic methods; and useful hints for good gas chromatography. As in previous editions, contributing authors have been chosen for their expertise and active participation in their respective areas. Modern Practice of Gas Chromatography, Fourth Edition presents a well-rounded and comprehensive overview of the current state of this important technology, providing a practical reference that will greatly appeal to both experienced chomatographers and novices. |
| agilent 6890 troubleshooting guide: Chromatographic Detectors Raymond P.W. Scott, 1996-07-17 Comprehensively covers the design, construction, and operation of gas chromatography, liquid chromatography, and thin-layer chromatography detectors--all in one convenient, up-to-date source. Emphasizes the essential use of common specifications to describe all detectors, allowing easy comparison of their attributes. |
| agilent 6890 troubleshooting guide: The Career Coward's Guide to Resumes Katy Piotrowski, 2008 This third book in the Career Coward's series helps readers tackle the Herculean task of assessing their strengths and creating a paper sales document about themselves. In her friendly and comforting style, career coach Katy Piotrowski walks readers though all the basic steps of writing a resume and cover letter that fairly promotes their strengths and experiences without sounding like bragging. You'll learn how to determine what to include on your resume for great results, develop your unique experiences into powerful resume content, follow a foolproof process for creating a resume that works, and write outstanding cover letters in just minutes |
| agilent 6890 troubleshooting guide: Direct Analysis in Real Time Mass Spectrometry Yiyang Dong, 2018-04-09 Clear, comprehensive, and state of the art, the groundbreaking book on the emerging technology of direct analysis in real time mass spectrometry Written by a noted expert in the field, Direct Analysis in Real Time Mass Spectrometry offers a review of the background and the most recent developments in DART-MS. Invented in 2005, DART-MS offers a wide range of applications for solving numerous analytical problems in various environments, including food science, forensics, and clinical analysis. The text presents an introduction to the history of the technology and includes information on the theoretical background, for exampleon the ionization mechanism. Chapters on sampling and coupling to different types of mass spectrometers are followed by a comprehensive discussion of a broad range of applications. Unlike most other ionization methods, DART does not require laborious sample preparation, as ionization takes place directly on the sample surface. This makes the technique especially attractive for applications in forensics and food science. Comprehensive in scope, this vital text: -Sets the standard on an important and emerging ionization technique -Thoroughly discusses all the relevant aspects from instrumentation to applications -Helps in solving numerous analytical problems in various applications, for example food science, forensics, environmental and clinical analysis -Covers mechanisms, coupling to mass spectrometers, and includes information on challenges and disadvantages of the technique Academics, analytical chemists, pharmaceutical chemists, clinical chemists, forensic scientists, and others will find this illuminating text a must-have resource for understanding the most recent developments in the field. |
| agilent 6890 troubleshooting guide: Analysis of Drugs of Abuse Rabi A. Musah, 2018-07-05 This volume features a comprehensive set of protocols featuring a range of both old and new technologies that can be used to analyze drugs of abuse, including prescription drugs, new psychoactive substances and psychoactive plants. Chapters guide readers through the application of color tests, light microscopy-based particle imaging, GC-MS, Raman spectroscopy, capillary electrophoresis, ultra-high performance LC-tandem MS, DART-MS, MALDI-mass spectrometry imaging, LC-MS/MS and HPLC-ESI-MS/MS to the analysis of abused drugs in wastewater, hair, urine and plant-derived materials, among other matrices. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Analysis of Drugs of Abuse aims to ensure successful results in the further study of this vital field. |
| agilent 6890 troubleshooting guide: Analytical Gas Chromatography Walter Jennings, 2012-12-02 Analytical Gas Chromatography is a free-standing introduction to and guide through the rapidly progressing field of analytical gas chromatography. The book is divided into 10 chapters that cover various aspects of analytical gas chromatography, from most advantageous column type to troubleshooting. The opening chapters of the book discuss the advantages of the open tubular column over the packed column. This topic is followed by significant chapters on various variables in the gas chromatographic process, including sample injection, stationary phase, carrier gas, and installation. The effect of changes in these variables on the solution elution order is also considered. A chapter also examines the influence of instrumental design features, such as excessive or unswept volumes in the flow path; suitability of the detection mode; and speed and fidelity of the data-handling equipment. The book also presents selected methods that have been employed to achieve better results for a given gas chromatographic problem. The application areas of gas chromatographic process, including food, flavor, fragrance, petroleum- and chemical-related, environment, biology, and medicine, are also presented. The concluding chapter addresses the basic troubleshooting knowledge and considers other chromatographic problems and methods for their rectification. |
| agilent 6890 troubleshooting guide: Metabolic Profiling Georgios A. Theodoridis, Helen G. Gika, Ian D. Wilson, 2019-04-30 This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field. |
| agilent 6890 troubleshooting guide: Methods for the Determination of Organic Compounds in Drinking Water , 1990 |
| agilent 6890 troubleshooting guide: The Australian Official Journal of Trademarks , 1906 |
| agilent 6890 troubleshooting guide: Hydrocarbon and Lipid Microbiology Protocols Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, 2016-12-16 This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes. |
| agilent 6890 troubleshooting guide: Advances in the Use of Liquid Chromatography Mass Spectrometry (LC-MS): Instrumentation Developments and Applications , 2018-01-02 Advances in the Use of Liquid Chromatography Mass Spectrometry (LC-MS): Instrumentation Developments and Application, Volume 79, highlights the most recent LC-MS evolutions through a series of contributions by world renowned scientists that will lead the readers through the most recent innovations in the field and their possible applications. Many authoritative books on LC-MS are already present in market, describing in detail the different interfaces and their principles of operation. This book focuses more on new trends, starting with the innovations of each technique, to the most progressive challenges of LC-MS. - Presents an understanding of the new advancements in LC and MS which are essential for a step forward in LC-MS applications - Provides insight into the state-of-the-art in the currently available LC-MS interfaces and their principle of use - Expounds on the new frontiers in LC-MS and their application potential |
| agilent 6890 troubleshooting guide: Comprehensive Guide to HILIC Waters Corporation, 2014-04-21 The Comprehensive Guide to HILIC: Hydrophilic Interaction Chromatography, a 72-page book, illustrates how HILIC works and how separation scientists can improve their success in separating and quantifying polar compounds in a variety of sample matrices. Looking for something else? Learn a new technique or technology with the Waters Primers Series, view other titles available here: http://www.wiley.com/go/waters |
| agilent 6890 troubleshooting guide: Guidelines on Representative Drug Sampling United Nations, 2009 The present guidelines describe a number of sampling methods, from arbitrary methods with a statistical background. They focus on sampling in cases where large numbers of relatively homogeneous material are available. They do not deal with so-called tactical sampling, which may be applied for house-searches or in clandestine laboratory investigations. Its aim is to support drug analysis laboratories in the selection of their sampling strategies and best working practices. |
| agilent 6890 troubleshooting guide: Fire Debris Analysis Eric Stauffer, Julia A. Dolan, Reta Newman, 2007-12-10 The study of fire debris analysis is vital to the function of all fire investigations, and, as such, Fire Debris Analysis is an essential resource for fire investigators. The present methods of analysis include the use of gas chromatography and gas chromatography-mass spectrometry, techniques which are well established and used by crime laboratories throughout the world. However, despite their universality, this is the first comprehensive resource that addresses their application to fire debris analysis.Fire Debris Analysis covers topics such as the physics and chemistry of fire and liquid fuels, the interpretation of data obtained from fire debris, and the future of the subject. Its cutting-edge material and experienced author team distinguishes this book as a quality reference that should be on the shelves of all crime laboratories. - Serves as a comprehensive guide to the science of fire debris analysis - Presents both basic and advanced concepts in an easily readable, logical sequence - Includes a full-color insert with figures that illustrate key concepts discussed in the text |
| agilent 6890 troubleshooting guide: Plant Secondary Metabolism Engineering Arthur Germano Fett-Neto, 2010-05-06 This book presents detailed practical information on important methods used in the engineering of plant secondary metabolism pathways and the acquisition of essential knowledge in performing this activity, including important advances and emerging strategies. |
| agilent 6890 troubleshooting guide: Plant Metabolomics Kazuki Saito, Richard A. Dixon, Lothar Willmitzer, 2006-06-29 Metabolomics – which deals with all metabolites of an organism – is a rapidly-emerging sector of post-genome research fields. It plays significant roles in a variety of fields from medicine to agriculture and holds a fundamental position in functional genomics studies and their application in plant biotechnology. This volume comprehensively covers plant metabolomics for the first time. The chapters offer cutting-edge information on analytical technology, bioinformatics and applications. They were all written by leading researchers who have been directly involved in plant metabolomics research throughout the world. Up-to-date information and future developments are described, thereby producing a volume which is a landmark of plant metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all plant science fields. |
| agilent 6890 troubleshooting guide: Immobilization of Enzymes and Cells Jose M. Guisan, Juan M. Bolivar, Fernando López-Gallego, Javier Rocha-Martín, 2021-01-29 This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field. |
| agilent 6890 troubleshooting guide: Algae Biotechnology Faizal Bux, Yusuf Chisti, 2016-03-09 This book examines the utilization of algae for the development of useful products and processes with the emphasis towards green technologies and processes, and the requirements to make these viable. Serving as a complete reference guide to the production of biofuels and other value added products from micro and macro algae, it covers various aspects of algal biotechnology from the basics to large scale cultivation, harvesting and processing for a variety of products. It is authored and edited by respected world experts in the field of algal biotechnology and provides the most up to date and cutting edge information on developments in the field. Over the past decade there has been substantial focus and related literature on the application of algal biomass for the generation of novel processes and products. ‘Algae Biotechnology: Products and Processes’ encompasses a holistic approach to critically evaluating developments in the field of algal biotechnology whilst taking into account recent advances and building on the body of knowledge. Aspects of the effects of harmful algae are also discussed, as well as the potential commercial application of algal biotechnology, the techno-economic feasibility of algal biodiesel production and the use of genetic and metabolic engineering for the improvement of yield. Other bioenergy sources such as alcohol fuels, aviation fuels, biohydrogen and biogas are also covered. This book is intended for postgraduates and researchers working in the biofuels and algal industry; it constitutes ideal reference material for both early stage and established researchers. |
| agilent 6890 troubleshooting guide: Laboratory Quality Assurance Manual Aiha Analytical Chemistry Committee, American Industrial Hygiene Association. Sampling and Laboratory Analysis Committee, 1997-12-01 |
| agilent 6890 troubleshooting guide: Porous Polymers Michael S. Silverstein, Neil R. Cameron, Marc A. Hillmyer, 2011-04-19 This book gathers the various aspects of the porous polymer field into one volume. It not only presents a fundamental description of the field, but also describes the state of the art for such materials and provides a glimpse into the future. Emphasizing a different aspect of the ongoing research and development in porous polymers, the book is divided into three sections: Synthesis, Characterization, and Applications. The first part of each chapter presents the basic scientific and engineering principles underlying the topic, while the second part presents the state of the art results based on those principles. In this fashion, the book connects and integrates topics from seemingly disparate fields, each of which embodies different aspects inherent in the diverse field of porous polymeric materials. |
| agilent 6890 troubleshooting guide: Hydrocarbon and Lipid Microbiology Protocols Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, 2016-06-28 This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology. It complements a second Volume that describes protocols for systems and synthetic biology applications. The wet experimental tools presented in this Volume include protocols for the standardisation of transcriptional measurements, application of uracil excision-based DNA editing for, inter alia, multi-gene assembly, the use of fluxomics to optimise “reducing power availability”, and the incorporation of non-canonical amino acids into proteins for optimisation of activities. Phenome-ing microbes, using a combination of RNA-seq and bioinformatic algorithms, is presented, as is an illustration, using methylotrophs as an example, of how the different key omics approaches constitute a pipeline for functional analysis, acquisition of a systems overview, and metabolic optimisation. Complementary computational tools that are presented include protocols for probing the genome architecture of regulatory networks, genome-scale metabolic reconstruction, and bioinformatic approaches to guide metabolic engineering. The Volume also includes an overview of how synthetic biology approaches can be used to improve biocontainment. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes. |
| agilent 6890 troubleshooting guide: The Dramatic Works of Thomas Heywood Now First Collected with Illustrative Notes and a Memoir of the Author in Six Volumes Thomas Heywood, 1874 |
| agilent 6890 troubleshooting guide: The Home Inspection Book Marcia Darvin Spada, 2007 THE HOME INSPECTION BOOK is designed to serve as a learning tool as well as a reference guide. This professional guide is a comprehensive yet self-paced handbook covering both the practice and the business of home inspection addressing all the key areas associated with the business of home inspection in this rapidly growing profession. In addition, this handbook also takes you step-by-step through the entire process of home inspection following the most current guidelines established by the American Society of Home Inspectors (ASHI), the National Association of Home Inspectors (NAHI) and the National Association of Certified Home Inspectors (NACHI). |
| agilent 6890 troubleshooting guide: Identification of Essential Oil Components by Gas Chromatography/quadrupole Mass Spectroscopy Robert P. Adams, 2001 |
| agilent 6890 troubleshooting guide: Beginners Guide to UPLC Waters Corporation, 2014-04-21 The Beginners Guide to UPLC: Ultra-Performance Liquid Chromatography, is a 52-page book designed to provide new, existing and potential UPLC users the ability to understand how UPLC technology works, how to be successful with it, and how it can provide impactful results within organizations. Looking for something else? Learn a new technique or technology with the Waters Primers Series, view other titles available here: http://www.wiley.com/go/waters |
| agilent 6890 troubleshooting guide: Fungal Secondary Metabolism Nancy P. Keller, Geoffrey Turner, 2012-10-14 Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties. Recent focus on fungal genomics coupled with advances in detection and molecular manipulation techniques has galvanized a revitalization of this field. Fungal Secondary Metabolism: Methods and Protocols is aimed at providing the key methodologies currently in use and necessary for accessing and exploiting the natural product information provided by the genomes of this large and varied kingdom. Written by active researchers in the field, the chapters deal with all the steps necessary, from optimization of fungal culture conditions for metabolite production, through rapid genome sequencing and bioinformatics, and genetic manipulations for functional analysis, to detection and testing of metabolites. In addition, chapters on basic science address approaches to the genetic regulation, protein biochemistry, and cellular localization of the biosynthetic pathways. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and hands-on, Fungal Secondary Metabolism: Methods and Protocols encourages new investigators to enter the field and expands upon the expertise and range of skills of those already researching fungal natural products. |
Capillary Column Installation Quick Reference Guide - Agilent …
Nov 10, 2022 · This Information Applies To: Agilent GC Systems Issue The data rate determines the number of sampling points across the chromatographic peak and the sample background. …
General Recommendations for Using an Agilent RID
Nov 10, 2022 · All Agilent degassers can be used except for G1379A and G1379B. Always have a thermostatted column compartment (TCC) / multicolumn thermostat (MCT) in your stack. …
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How to Bake-out the Split/Splitless Inlet (S/SL) on Agilent GC …
Nov 10, 2022 · This Information Applies To: Agilent 7697A headspace sampler. Issue: The headspace sampler may present a flow shutdown or poor chromatography with decreased …
How to Check, Configure and Use the IP Address of an Agilent LC …
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Jul 17, 2024 · This Information Applies To: Agilent 6850, 6890, 7820, 7890, 8850, 8860, 8890, Intuvo 9000 GC Systems with a Thermal Conductivity Detector (TCD) Issue The TCD …
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Oct 13, 2023 · This information applies to: Agilent 6400 Triple Quadrupole LC/MS systems, MassHunter Workstation 10.1 or earlier. Issue The MassHunter Optimizer tool allows users to …
Capillary Column Installation Quick Reference Guide - Agilent Technol…
Nov 10, 2022 · This Information Applies To: Agilent GC Systems Issue The data rate determines the number of sampling points across the chromatographic peak and the …
General Recommendations for Using an Agilent RID
Nov 10, 2022 · All Agilent degassers can be used except for G1379A and G1379B. Always have a thermostatted column compartment (TCC) / multicolumn thermostat (MCT) in …
4150 and 4200 TapeStation - Wiki - Automated Electrophoresis - Agile…
Here is a collection of Agilent 4150 and 4200 TapeStation Resources. Links to user manuals , application resources and technical notes , quick guides , software
How to Bake-out the Split/Splitless Inlet (S/SL) on Agilent GC Systems
Nov 10, 2022 · This Information Applies To: Agilent 7697A headspace sampler. Issue: The headspace sampler may present a flow shutdown or poor chromatography with …
How to Check, Configure and Use the IP Address of an Agilent LC Mo…
Nov 10, 2022 · Agilent HPLC modules can be configured to use either a default IP address (192.168.254.11) that cannot be changed (static), or a stored IP address that can be …
