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# Agilent GC Column Selection Guide: A Comprehensive Guide for Optimal Chromatography
Author: Dr. Emily Carter, PhD, Analytical Chemist with 15 years of experience in gas chromatography, specializing in method development and optimization at a leading pharmaceutical company.
Publisher: Chromatography Solutions Inc., a leading provider of chromatography supplies, training, and consulting services with over 20 years of experience in the field.
Editor: Dr. John Davis, PhD, Professor of Analytical Chemistry at the University of California, Berkeley, with expertise in gas chromatography and separation science.
Summary: This Agilent GC column selection guide provides a comprehensive overview of choosing the right GC column for your specific analytical needs. It covers key considerations like stationary phase selection, column dimensions, film thickness, and temperature limits. The guide also addresses common pitfalls and best practices for achieving optimal chromatographic performance and discusses the impact of column selection on method development and validation. Finally, it includes a FAQ section to address common queries and further reading resources.
1. Understanding Your Analytical Needs: The Foundation of Agilent GC Column Selection
Before embarking on the Agilent GC column selection process, a thorough understanding of your analytical goals is paramount. Several key factors need careful consideration:
Analyte Properties: The physical and chemical properties of your target analytes, such as polarity, boiling point, and thermal stability, directly influence column selection. Polar analytes require polar stationary phases, while non-polar analytes are best separated on non-polar columns. Volatile analytes may need shorter columns or lower temperatures, while thermally labile compounds require specialized columns and conditions.
Sample Matrix: The complexity of your sample matrix impacts column choice. A clean sample might allow the use of a less selective column, whereas a complex matrix might necessitate a highly selective column to achieve adequate separation.
Desired Resolution and Sensitivity: The required level of separation between analytes (resolution) and the detection limits (sensitivity) influence the column's dimensions (length, internal diameter), stationary phase film thickness, and potentially the use of specialized detectors.
Throughput Requirements: High-throughput analysis might favor shorter, faster columns, while achieving high resolution might require longer columns.
Regulatory Compliance: For regulated industries, compliance with specific standards and guidelines is crucial. The choice of column might be dictated by the regulatory requirements of the analysis.
2. Agilent GC Column Stationary Phases: A Detailed Overview
Agilent offers a wide variety of GC columns with diverse stationary phases. The selection of the stationary phase is the most critical aspect of Agilent GC column selection. Common stationary phases include:
Polysiloxanes (Silicone-based): These are the most common stationary phases and are available with varying degrees of polarity (e.g., methyl silicone, 5% phenyl methyl silicone, 50% phenyl methyl silicone). The percentage of phenyl groups determines the polarity of the phase.
PEG (Polyethylene Glycol): Highly polar stationary phase useful for separating polar compounds.
Wax-based columns: These are typically used for separating volatile compounds with low boiling points.
The choice of stationary phase depends primarily on the polarity of your analytes. A "like dissolves like" principle is applied; polar analytes are better separated on polar stationary phases, and non-polar analytes on non-polar stationary phases.
3. Agilent GC Column Dimensions and Film Thickness: Optimizing Performance
Beyond stationary phase, column dimensions and film thickness play a crucial role in Agilent GC column selection.
Column Length: Longer columns provide greater separation efficiency but increase analysis time.
Column Internal Diameter (ID): Narrower ID columns improve sensitivity but may require higher carrier gas flow rates.
Film Thickness: Thicker films increase capacity but reduce efficiency. Thinner films improve resolution but may have lower capacity. Careful consideration of these parameters is essential for optimizing separation and speed.
4. Temperature Limits and Operational Considerations: Ensuring Column Longevity
Each Agilent GC column has specific temperature limits. Exceeding these limits can damage the stationary phase, leading to poor performance and column failure. Careful consideration of the temperature program, including maximum temperatures, ramp rates, and isothermal holds, is essential.
5. Common Pitfalls in Agilent GC Column Selection and Best Practices
Ignoring analyte properties: Choosing a column without considering analyte polarity is a frequent mistake.
Incorrect film thickness selection: Using the wrong film thickness can lead to poor resolution or peak tailing.
Exceeding temperature limits: Overheating the column can damage the stationary phase and shorten column lifetime.
Improper column installation: Incorrect installation can lead to poor peak shapes and reduced sensitivity.
Insufficient conditioning: Not properly conditioning a new column can affect peak shape and retention times.
6. Method Development and Validation: The Role of Agilent GC Column Selection
The choice of Agilent GC column significantly impacts method development and validation. Careful selection ensures the method meets the required sensitivity, selectivity, and robustness.
7. Choosing the Right Agilent GC Column: A Step-by-Step Approach
This Agilent GC column selection guide recommends a systematic approach:
1. Analyze your analytes: Understand their properties and polarity.
2. Consider your sample matrix: Assess its complexity.
3. Define your resolution and sensitivity requirements: Determine the level of separation and detection limits needed.
4. Evaluate throughput needs: Consider the analysis time required.
5. Consult Agilent's column selection guides and databases: Utilize the resources available from Agilent Technologies.
6. Test different columns: Experimentation is essential to optimize your method.
Conclusion
Selecting the appropriate Agilent GC column is crucial for achieving optimal chromatographic performance. This guide highlights the key factors influencing column selection, common pitfalls to avoid, and best practices for ensuring successful analysis. Careful consideration of analyte properties, sample matrix, desired resolution, and column dimensions is essential for optimizing separation efficiency and method robustness. By following the recommendations outlined in this Agilent GC column selection guide, analysts can significantly improve their chromatographic results and achieve accurate and reliable data.
FAQs
1. What is the difference between DB-1 and DB-5 columns? DB-1 is a 100% dimethyl polysiloxane (non-polar), while DB-5 is a 5% phenyl, 95% dimethyl polysiloxane (slightly polar).
2. How do I determine the appropriate film thickness for my Agilent GC column? The film thickness choice depends on the analyte concentration and desired resolution. Thinner films offer better resolution for trace analysis, while thicker films are suitable for higher concentration samples.
3. How often should I replace my Agilent GC column? Column lifetime varies depending on usage, but signs of degradation include decreased resolution, peak tailing, and baseline noise. Regular performance checks are recommended.
4. How do I condition a new Agilent GC column? Conditioning involves heating the column to a specified temperature under a carrier gas flow to remove any residual solvents or impurities.
5. What is the impact of carrier gas flow rate on Agilent GC column performance? The carrier gas flow rate affects peak broadening, retention time, and sensitivity. Optimizing flow rate is essential for achieving optimal results.
6. How do I choose between packed and capillary columns? Capillary columns offer better resolution and sensitivity than packed columns and are preferred for most modern GC applications.
7. What is the role of the stationary phase in Agilent GC column separation? The stationary phase is responsible for separating the analytes based on their interactions with the stationary phase. The polarity of the stationary phase determines the selectivity of the separation.
8. What is the difference between isothermal and temperature-programmed GC? Isothermal GC maintains a constant column temperature, while temperature-programmed GC varies the temperature over time to optimize separation of analytes with a wide range of boiling points.
9. How do I troubleshoot poor peak shape in my Agilent GC analysis? Poor peak shape may be caused by various factors including column degradation, injector problems, detector issues, or improper sample preparation. Systematic troubleshooting is necessary to identify the cause.
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| agilent gc column selection guide: GC Inlets Matthew S. Klee, 1990-02-01 |
| agilent gc column selection guide: Basic Gas Chromatography Harold M. McNair, James M. Miller, 2011-09-20 The New Edition of the Well-Regarded Handbook on Gas Chromatography Since the publication of the highly successful first edition of Basic Gas Chromatography, the practice of chromatography has undergone several notable developments. Basic Gas Chromatography, Second Edition covers the latest in the field, giving readers the most up-to-date guide available, while maintaining the first edition's practical, applied approach to the subject and its accessibility to a wide range of readers. The text provides comprehensive coverage of basic topics in the field, such as stationary phases, packed columns and inlets, capillary columns and inlets, detectors, and qualitative and quantitative analysis. At the same time, the coverage also features key additions and updated topics including: Gas chromatography-mass spectrometry (GC-MS) Sampling methods Multidimensional gas chromatography Fast gas chromatography Gas chromatography analysis of nonvolatile compounds Inverse gas chromatography and pyrolysis gas chromatography Along with these new and updated topics, the references, resources, and Web sites in Basic Gas Chromatography have been revised to reflect the state of the field. Concise and fundamental in its coverage, Basic Gas Chromatography, Second Edition remains the standard handbook for everyone from undergraduates studying analytical chemistry to working industrial chemists. |
| agilent gc column selection guide: Preparative Liquid Chromatography B.A. Bidlingmeyer, 1987-07-01 This volume provides a straightforward approach to isolation and purification problems with a thorough presentation of preparative LC strategy including the interrelationship between the input and output of the instrumentation, while keeping to an application focus.The book stresses the practical aspects of preparative scale separations from TLC isolations through various laboratory scale column separations to very large scale production. It also gives a thorough description of the performance parameters (e.g. throughput, separation quality, etc.) as a function of operational parameters (e.g. particle size, column size, solvent usage, etc.). Experts in the field have contributed a well balanced presentation of separation development strategies from preparative TLC to commercial preparative process with practical examples in a wide variety of application areas such as drugs, proteins, nucleotides, industrial extracts, organic chemicals, enantiomers, polymers, etc. |
| agilent gc column selection guide: Portable Spectroscopy and Spectrometry, Applications Richard A. Crocombe, Pauline E. Leary, Brooke W. Kammrath, 2021-04-28 The most comprehensive resource available on the many applications of portable spectrometers, including material not found in any other published work Portable Spectroscopy and Spectrometry: Volume Two is an authoritative and up-to-date compendium of the diverse applications for portable spectrometers across numerous disciplines. Whereas Volume One focuses on the specific technologies of the portable spectrometers themselves, Volume Two explores the use of portable instruments in wide range of fields, including pharmaceutical development, clinical research, food analysis, forensic science, geology, astrobiology, cultural heritage and archaeology. Volume Two features contributions by a multidisciplinary team of experts with hands-on experience using portable instruments in their respective areas of expertise. Organized both by instrumentation type and by scientific or technical discipline, 21 detailed chapters cover various applications of portable ion mobility spectrometry (IMS), infrared and near-infrared (NIR) spectroscopy, Raman and x-ray fluorescence (XRF) spectroscopy, smartphone spectroscopy, and many others. Filling a significant gap in literature on the subject, the second volume of Portable Spectroscopy and Spectrometry: Features a significant amount of content published for the first time, or not available in existing literature Brings together work by authors with assorted backgrounds and fields of study Discusses the central role of applications in portable instrument development Covers the algorithms, calibrations, and libraries that are of critical importance to successful applications of portable instruments Includes chapters on portable spectroscopy applications in areas such as the military, agriculture and feed, hazardous materials (HazMat), art conservation, and environmental science Portable Spectroscopy and Spectrometry: Volume Two is an indispensable resource for developers of portable instruments in universities, research institutes, instrument companies, civilian and government purchasers, trainers, operators of portable instruments, and educators and students in portable spectroscopy courses. |
| agilent gc column selection guide: Practical HPLC Method Development Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch, 2012-12-03 This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations. |
| agilent gc column selection guide: Practical HPLC Methodology and Applications Brian A. Bidlingmeyer, 1993-05-06 Of related interest. Trace and Ultratrace Analysis by HPLC Satinder Ahuja Written by a leading scientist in the field, this monograph provides the first definitive and technically up-to-date treatment of the theory, equipment, and applications of chemistry's most powerful reliable analytical technique. Coverage includes an encyclopedic compendium of common substances that require trace and ultratrace analysis, and features clear discussion of such important topics as considerations for HPLC equipment, sensitive detectors, sample preparation, method development, selectivity and computer-based optimizations, optimizing detectability, and much more. 1991 (0 471-51419-5) 432 pp. High Performance Liquid Chromatography in Biotechnology Edited by William S. Hancock Analytical chemists, biochemists, and chemical engineers will find this up-to-date guide to HPLC's recent developments essential for enhancing on-the-job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as those in monoclonal antibody and nucleic acid purification. Techniques for quality control programs and advanced technology are also discussed. 1990 (0 471-82584-0) 564 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together for the first time the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physical-mathematical form, facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide background material applicable to a wide range of separation methods, while the final five chapters illustrate specific techniques and methods. 1991 (0 471-52089-6) 320 pp. |
| agilent gc column selection guide: Porous Polymers Michael S. Silverstein, Neil R. Cameron, Marc A. Hillmyer, 2011-04-19 This book gathers the various aspects of the porous polymer field into one volume. It not only presents a fundamental description of the field, but also describes the state of the art for such materials and provides a glimpse into the future. Emphasizing a different aspect of the ongoing research and development in porous polymers, the book is divided into three sections: Synthesis, Characterization, and Applications. The first part of each chapter presents the basic scientific and engineering principles underlying the topic, while the second part presents the state of the art results based on those principles. In this fashion, the book connects and integrates topics from seemingly disparate fields, each of which embodies different aspects inherent in the diverse field of porous polymeric materials. |
| agilent gc column selection guide: Processing Metabolomics and Proteomics Data with Open Software Robert Winkler, 2020-03-19 Metabolomics and proteomics allow deep insights into the chemistry and physiology of biological systems. This book expounds open-source programs, platforms and programming tools for analysing metabolomics and proteomics mass spectrometry data. In contrast to commercial software, open-source software is created by the academic community, which facilitates the direct interaction between users and developers and accelerates the implementation of new concepts and ideas. The first section of the book covers the basics of mass spectrometry, experimental strategies, data operations, the open-source philosophy, metabolomics, proteomics and statistics/ data mining. In the second section, active programmers and users describe available software packages. Included tutorials, datasets and code examples can be used for training and for building custom workflows. Finally, every reader is invited to participate in the open science movement. |
| agilent gc column selection guide: Practical Gas Chromatography Katja Dettmer-Wilde, Werner Engewald, 2014-11-05 Gas chromatography continues to be one of the most widely used analytical techniques, since its applications today expand into fields such as biomarker research or metabolomics. This new practical textbook enables the reader to make full use of gas chromatography. Essential fundamentals and their implications for the practical work at the instrument are provided, as well as details on the instrumentation such as inlet systems, columns and detectors. Specialized techniques from all aspects of GC are introduced ranging from sample preparation, solvent-free injection techniques, and pyrolysis GC, to separation including fast GC and comprehensive GCxGC and finally detection, such as GC-MS and element-specific detection. Various fields of application such as enantiomer, food, flavor and fragrance analysis, physicochemical measurements, forensic toxicology, and clinical analysis are discussed as well as cutting-edge application in metabolomics is covered. |
| agilent gc column selection guide: The HPLC Expert II Stavros Kromidas, 2017-01-05 How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful HPLC Expert in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications. |
| agilent gc column selection guide: Static Headspace-Gas Chromatography Bruno Kolb, Leslie S. Ettre, 2006-04-20 STATIC HEADSPACE-GAS CHROMATOGRAPHY THE ONLY REFERENCE TO PROVIDE BOTH CURRENT AND THOROUGH COVERAGE OF THIS IMPORTANT ANALYTICAL TECHNIQUE Static headspace-gas chromatography (HS-GC) is an indispensable technique for analyzing volatile organic compounds, enabling the analyst to assay a variety of sample matrices while avoiding the costly and time-consuming preparation involved with traditional GC. Static Headspace-Gas Chromatography: Theory and Practice has long been the only reference to provide in-depth coverage of this method of analysis. The Second Edition has been thoroughly updated to reflect the most recent developments and practices, and also includes coverage of solid-phase microextraction (SPME) and the purge-and-trap technique. Chapters cover: Principles of static and dynamic headspace analysis, including the evolution of HS-GC methods and regulatory methods using static HS-GC Basic theory of headspace analysis—physicochemical relationships, sensitivity, and the principles of multiple headspace extraction HS-GC techniques—vials, cleaning, caps, sample volume, enrichment, and cryogenic techniques Sample handling Cryogenic HS-GC Method development in HS-GC Nonequilibrium static headspace analysis Determination of physicochemical functions such as vapor pressures, activity coefficients, and more Comprehensive and focused, Static Headspace-Gas Chromatography, Second Edition provides an excellent resource to help the reader achieve optimal chromatographic results. Practical examples with original data help readers to master determinations in a wide variety of areas, such as forensic, environmental, pharmaceutical, and industrial applications. |
| agilent gc column selection guide: Modern HPLC for Practicing Scientists Michael W. Dong, 2016-04-06 A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and greener methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. |
| agilent gc column selection guide: Handbook of Essential Oils K. Husnu Can Baser, Gerhard Buchbauer, 2009-12-28 Egyptian hieroglyphs, Chinese scrolls, and Ayurvedic literature record physicians administering aromatic oils to their patients. Today society looks to science to document health choices and the oils do not disappoint. The growing body of evidence of their efficacy for more than just scenting a room underscores the need for production standards, quality control parameters for raw materials and finished products, and well-defined Good Manufacturing Practices. Edited by two renowned experts, the Handbook of Essential Oils covers all aspects of essential oils from chemistry, pharmacology, and biological activity, to production and trade, to uses and regulation. Bringing together significant research and market profiles, this comprehensive handbook provides a much-needed compilation of information related to the development, use, and marketing of essential oils, including their chemistry and biochemistry. A select group of authoritative experts explores the historical, biological, regulatory, and microbial aspects. This reference also covers sources, production, analysis, storage, and transport of oils as well as aromatherapy, pharmacology, toxicology, and metabolism. It includes discussions of biological activity testing, results of antimicrobial and antioxidant tests, and penetration-enhancing activities useful in drug delivery. New information on essential oils may lead to an increased understanding of their multidimensional uses and better, more ecologically friendly production methods. Reflecting the immense developments in scientific knowledge available on essential oils, this book brings multidisciplinary coverage of essential oils into one all-inclusive resource. |
| agilent gc column selection guide: Handbook of GC/MS Hans-Joachim Hübschmann, 2008-12-03 This is the first comprehensive reference work for GC/MS now in its second edition. It offers broad coverage, from sample preparation to the evaluation of MS-Data, including library searches. Fundamentals, techniques, and applications are described. A large part of the book is devoted to numerous examples for GC/MS-applications in environmental, food, pharmaceutical and clinical analysis. These proven examples come from the daily practice of various laboratories. The book also features a glossary of terms and a substance index that helps the reader to find information for his particular analytical problem. The author presents in a consistent and clear style his experience from numerous user workshops which he has organized. This is a thoroughly revised and updated English edition based on an edition which was highly successful in Germany. |
| agilent gc column selection guide: Modern Practice of Gas Chromatography Robert L. Grob, PhD, Eugene F. Barry, 2004-08-04 The bible of gas chromatography-offering everything the professional and the novice need to know about running, maintaining, and interpreting the results from GC Analytical chemists, technicians, and scientists in allied disciplines have come to regard Modern Practice of Gas Chomatography as the standard reference in gas chromatography. In addition to serving as an invaluable reference for the experienced practitioner, this bestselling work provides the beginner with a solid understanding of gas chromatographic theory and basic techniques. This new Fourth Edition incorporates the most recent developments in the field, including entirely new chapters on gas chromatography/mass spectrometry (GC/MS); optimization of separations and computer assistance; high speed or fast gas chromatography; mobile phase requirements: gas system requirements and sample preparation techniques; qualitative and quantitative analysis by GC; updated information on detectors; validation and QA/QC of chromatographic methods; and useful hints for good gas chromatography. As in previous editions, contributing authors have been chosen for their expertise and active participation in their respective areas. Modern Practice of Gas Chromatography, Fourth Edition presents a well-rounded and comprehensive overview of the current state of this important technology, providing a practical reference that will greatly appeal to both experienced chomatographers and novices. |
| agilent gc column selection guide: Split and Splitless Injection for Quantitative Gas Chromatography Konrad Grob, 2008-11-21 This comprehensive and unique handbook of split and splitless injection techniques has been completely revised and updated. This new edition offers: - New insights concerning sample evaporation in the injector - Information about matrix effects - A new chapter on injector design The real processes within the injector are for the first time visualized and explained by the CD-ROM included in the book. Furthermore the reader will understand the concepts of injection techniques and get a knowledge of the sources of error. The handbook also includes many practical guidelines. From reviews of former editions: This substantial book is on injection techniques alone, which ... demonstrates this can have many pitfalls ... no one should be allowed to direct a laboratory doing quantitative analysis by GC without first being thoroughly familar with this book ... The Analyst This is a detailed reference volume filled with practical suggestions and techniques for managing split and splitless injection in the day-to-day world of the working gas chromatographer. It will be useful ... for anyone who must work hands-on with GC. Journal of High Resolution Chromatography |
| agilent gc column selection guide: Packed Column SFC T A Berger, 2007-10-31 Packed Column SFC is the third title to be published in this series and has been produced as a result of the dramatic re-emergence, in the last three years, of packed column instrumentation. This has led to a redefinition of the technique and an urgent need for a practical guide that deals with its subtleties. This book fulfils that need and deals exclusively with packed column SFC. It places the emphasis on understanding the underlying chemistry in order to perform rapid, systematic optimizations and provides many practical tips to help the new user avoid problems unique to SFC. It also proposes a detailed scheme for method development and provides lists of prioritized guidelines. The book clears up some of the confusion that surrounds the analytical use of supercritical fluids and assists the user in understanding the power and utility of this technique. Detailed chapters cover the most promising new application areas for packed column SFC, which are often overlooked in the mainstream chromatography literature. Like the other books in this popular series, Packed Column SFC will prove an invaluable guide and is essential reading for graduates, postgraduates and researchers with interests in pharmaceuticals, agricultural chemistry, small polar drug molecules, chiral analysis, environmental chemistry, and chromatography/instrumentation. |
| agilent gc column selection guide: HPLC Columns Uwe D. Neue, 1997-07-28 An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include: * Comprehensive overview of state-of-the-art HPLC column technology * Explanation of the underlying principles of HPLC columns * Methods for selecting columns * Practical advice on using and applying columns, including examples * Section by M. Zoubair El Fallah on methods development * Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed * Troubleshooting section HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses. |
| agilent gc column selection guide: Advances in the Use of Liquid Chromatography Mass Spectrometry (LC-MS): Instrumentation Developments and Applications , 2018-01-02 Advances in the Use of Liquid Chromatography Mass Spectrometry (LC-MS): Instrumentation Developments and Application, Volume 79, highlights the most recent LC-MS evolutions through a series of contributions by world renowned scientists that will lead the readers through the most recent innovations in the field and their possible applications. Many authoritative books on LC-MS are already present in market, describing in detail the different interfaces and their principles of operation. This book focuses more on new trends, starting with the innovations of each technique, to the most progressive challenges of LC-MS. - Presents an understanding of the new advancements in LC and MS which are essential for a step forward in LC-MS applications - Provides insight into the state-of-the-art in the currently available LC-MS interfaces and their principle of use - Expounds on the new frontiers in LC-MS and their application potential |
| agilent gc column selection guide: Handbook of Pharmaceutical Analysis by HPLC Satinder Ahuja, Michael Dong, 2005-02-09 High pressure liquid chromatography–frequently called high performance liquid chromatography (HPLC or, LC) is the premier analytical technique in pharmaceutical analysis and is predominantly used in the pharmaceutical industry. Written by selected experts in their respective fields, the Handbook of Pharmaceutical Analysis by HPLC Volume 6, provides a complete yet concise reference guide for utilizing the versatility of HPLC in drug development and quality control. Highlighting novel approaches in HPLC and the latest developments in hyphenated techniques, the book captures the essence of major pharmaceutical applications (assays, stability testing, impurity testing, dissolution testing, cleaning validation, high-throughput screening). A complete reference guide to HPLC Describes best practices in HPLC and offers 'tricks of the trade' in HPLC operation and method development Reviews key HPLC pharmaceutical applications and highlights currents trends in HPLC ancillary techniques, sample preparations, and data handling |
| agilent gc column selection guide: Preparative and Production Scale Chromatography G. Ganetsos, P.E. Barker, 1992-09-16 Describes the latest developments in the scaling-up and application of chromatographic operations and demonstrates that production-scale chromatography is a powerful and invaluable separation process. The book covers every important process design and reveals actual, immediately applicable techniques and is designed to appeal to design, chemical/biochemical, and research and development engineers, process development managers, bioprocess technologists, analytical and clinical chemists and biochemists, pharmacists, and upper-level undergraduate, graduate, and continuing-education students in these disciplines. |
| agilent gc column selection guide: Fire Debris Analysis Eric Stauffer, Julia A. Dolan, Reta Newman, 2007-12-10 The study of fire debris analysis is vital to the function of all fire investigations, and, as such, Fire Debris Analysis is an essential resource for fire investigators. The present methods of analysis include the use of gas chromatography and gas chromatography-mass spectrometry, techniques which are well established and used by crime laboratories throughout the world. However, despite their universality, this is the first comprehensive resource that addresses their application to fire debris analysis.Fire Debris Analysis covers topics such as the physics and chemistry of fire and liquid fuels, the interpretation of data obtained from fire debris, and the future of the subject. Its cutting-edge material and experienced author team distinguishes this book as a quality reference that should be on the shelves of all crime laboratories. - Serves as a comprehensive guide to the science of fire debris analysis - Presents both basic and advanced concepts in an easily readable, logical sequence - Includes a full-color insert with figures that illustrate key concepts discussed in the text |
| agilent gc column selection guide: HPLC and UHPLC for Practicing Scientists Michael W. Dong, 2019-07-23 A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher. |
| agilent gc column selection guide: Advanced Gas Chromatography Mustafa Ali Mohd, 2012-03-21 Progress in agricultural, biomedical and industrial applications' is a compilation of recent advances and developments in gas chromatography and its applications. The chapters cover various aspects of applications ranging from basic biological, biomedical applications to industrial applications. Book chapters analyze new developments in chromatographic columns, microextraction techniques, derivatisation techniques and pyrolysis techniques. The book also includes several aspects of basic chromatography techniques and is suitable for both young and advanced chromatographers. It includes some new developments in chromatography such as multidimensional chromatography, inverse chromatography and some discussions on two-dimensional chromatography. The topics covered include analysis of volatiles, toxicants, indoor air, petroleum hydrocarbons, organometallic compounds and natural products. The chapters were written by experts from various fields and clearly assisted by simple diagrams and tables. This book is highly recommended for chemists as well as non-chemists working in gas chromatography. |
| agilent gc column selection guide: Selective Detectors Robert E. Sievers, 1995-04-03 A timely and authoritative review of the current state of selective detector technology This book was written for professionals who need to keep abreast of the latest developments and emerging trends in selective detectors and their applications. It comprises contributions from many of the leading innovators and pioneers in the field, including James Lovelock, inventor of the electron capture detector, whose own contribution is certain to be a rich source of ideas and inspiration for all who read it. Offering a balanced presentation of theory and practice, Selective Detectors: Reviews the theory and underlying principles of a broad range of devices Discusses, in detail, capabilities and current applications, with an emphasis on interdisciplinary applications, including environmental, petrochemical, biomedical, and quality control Explores, in depth, the latest advances and emerging technologies Arms readers with a wealth of practical how-to information on selecting, using, modifying, and building selective detectors for a wide range of applications Future historians studying the late twentieth century will almost certainly come to view the advent of selective detectors as among the truly formative technological developments of the period. Anyone who doubts this thesis need only consider the impact of selective detection on environmental quality, the sciences, technology, medicine, business and industry, public policy, quality control, and many other fields. Yet, despite the obvious importance of selective detectors, there continues to be a scarcity of books dedicated to helping professionals keep abreast of the latest developments and emerging trends in this in fluential technology. This timely and authoritative review of the current state of selective detector technology fills that gap. This book focuses on the newest selective detectors for chromatographic analysis. Conceived and shepherded into existence by a major figure in analytical chemistry and environmental analysis, it includes contributions from many of the leading innovators and pioneers in the field. Most prominent among these is Dr. James Lovelock, inventor of the electron capture detector, whose chapter on the history and development of selective detectors will be a rich source of ideas and inspiration for all who read it. Offering a balanced presentation of theory and practice, Selective Detectors reviews the theory and underlying principles of selective detectors; discusses, in detail, their current capabilities and applications; explores the latest advances and emerging technologies; and arms readers with a wealth of practical how-to information on selecting, using, modifying, and building selective detectors for a wide range of applications. Selective Detectors is an invaluable resource for analytical chemists and technicians working in a variety of disciplines, including environmental science, petrochemical industries, the food and beverage industries, biotechnology, medicine, and more. |
| agilent gc column selection guide: Gas Chromatography Peter Kusch, 2019-09-04 Gas chromatography (GC) is one of the most important types of chromatography used in analytical chemistry for separating and analyzing chemical organic compounds. Today, gas chromatography is one of the most widespread investigation methods of instrumental analysis. This technique is used in the laboratories of chemical, petrochemical, and pharmaceutical industries, in research institutes, and also in clinical, environmental, and food and beverage analysis. This book is the outcome of contributions by experts in the field of gas chromatography and includes a short history of gas chromatography, an overview of derivatization methods and sample preparation techniques, a comprehensive study on pyrazole mass spectrometric fragmentation, and a GC/MS/MS method for the determination and quantification of pesticide residues in grape samples. |
| agilent gc column selection guide: Biochemical Analysis Tools Oana-Maria Boldura, Cornel Balta, Prof Nasser Awwad, 2020-06-24 This book explores the role of nucleic acid analysis and the advances it has led to in the field of life sciences. The first section is a collection of chapters covering experimental methods used in molecular biology, the techniques adjacent to these methods, and the steps of analysis before and after obtaining raw DNA data. The second section deals with the principles of chromatography, method development, sample preparation, and industrial applications. |
| agilent gc column selection guide: Modern Size-Exclusion Liquid Chromatography Andre Striegel, Wallace W. Yau, Joseph J. Kirkland, Donald D. Bly, 2009-07-31 The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods. |
| agilent gc column selection guide: Amino Acid Analysis Michail A. Alterman, Peter Hunziker, 2011-12-02 Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences. |
| agilent gc column selection guide: Comprehensive Guide to HILIC Waters Corporation, 2014-04-21 The Comprehensive Guide to HILIC: Hydrophilic Interaction Chromatography, a 72-page book, illustrates how HILIC works and how separation scientists can improve their success in separating and quantifying polar compounds in a variety of sample matrices. Looking for something else? Learn a new technique or technology with the Waters Primers Series, view other titles available here: http://www.wiley.com/go/waters |
| agilent gc column selection guide: HPLC of Polymers Harald Pasch, Bernd Trathnigg, 2012-12-06 Polymers are mainly characterized by molar mass, chemical composition, functionality and architecture. The determination of the complex structure of polymers by chromatographic and spectroscopic methods is one of the major concerns of polymer analysis and characterization. This lab manual describes the experimental approach to the chromatographic analysis of polymers. Different chromatographic methods, their theoretical background, equipment, experimental procedures and applications are discussed. The book will enable polymer chemists, physicists and material scientists as well as students of macromolecular and analytical science to optimize chromatographic conditions for a specific separation problem. Special emphasis is given to the description of applications for homo- and copolymers and polymer blends. |
| agilent gc column selection guide: Comprehensive Organic Chemistry Experiments for the Laboratory Classroom Carlos A. M. Afonso, Nuno R. Candeias, Dulce Pereira Simão, Alexandre F. Trindade, Jaime A. S. Coelho, Bin Tan, Robert Franzén, 2016-12-16 This expansive and practical textbook contains organic chemistry experiments for teaching in the laboratory at the undergraduate level covering a range of functional group transformations and key organic reactions.The editorial team have collected contributions from around the world and standardized them for publication. Each experiment will explore a modern chemistry scenario, such as: sustainable chemistry; application in the pharmaceutical industry; catalysis and material sciences, to name a few. All the experiments will be complemented with a set of questions to challenge the students and a section for the instructors, concerning the results obtained and advice on getting the best outcome from the experiment. A section covering practical aspects with tips and advice for the instructors, together with the results obtained in the laboratory by students, has been compiled for each experiment. Targeted at professors and lecturers in chemistry, this useful text will provide up to date experiments putting the science into context for the students. |
| agilent gc column selection guide: Beginner's Guide to Size-Exclusion Chromatography Waters Corporation, 2014-04-21 This book is designed to help you explore and understand a very powerful tool in sample preparation technology: solid-phase extraction [SPE]. You will see how this technology, which uses devices with chromatographic packing material, can help meet your analytical challenges. Looking for something else? Learn a new technique or technology with the Waters Primers Series, view other titles available here: http://www.wiley.com/go/waters |
| agilent gc column selection guide: More Practical Problem Solving in HPLC Stavros Kromidas, 2008-01-08 A unique approach to solving HPLC problems. Everyone who bought Problem Solving in HPLC by Stavros Kromidas will equally benefit from nearly 100 new practical examples for optimization, trouble-shooting, and instrument performance given in this sequel. The author provides - guidance for selecting and evaluating methods, intstruments and columns, - practical help with everyday trouble-shooting, - advice for optimizing separations, always explaining the reason why. In each case the problem, the solution and the conclusions are presented over a maximum of 4 pages, and in addition the book contains manufacturers' addresses, references, data tables and checklists. |
| agilent gc column selection guide: Modern Sample Preparation for Chromatography Serban C. Moldoveanu, Victor David, 2021-02-24 Modern Sample Preparation for Chromatography, Second Edition explains the principles of sample preparation for chromatographic analysis. A variety of procedures are applied to make real-world samples amenable for chromatographic analysis and to improve results. This book's authors discuss each procedure's advantages, disadvantages and their applicability to different types of samples, along with their fit for different types of chromatographic analysis. The book contains numerous literature references and examples of sample preparation for different matrices and new sections on green approaches in sample preparation, progress in automation of sample preparation, non-conventional solvents for LLE (ionic liquids, deep eutectic mixtures, and others), and more. - Presents numerous techniques applied for sample preparation for chromatographic analysis - Provides an up-to-date source of information regarding the progress made in sample preparation for chromatography - Describes examples for specific types of matrices, providing a guide for choosing the appropriate sample preparation method for a given analysis |
| agilent gc column selection guide: Handbook of Solid Phase Microextraction Janusz Pawliszyn, 2011-12-01 The relatively new technique of solid phase microextraction (SPME) is an important tool to prepare samples both in the lab and on-site. SPME is a green technology because it eliminates organic solvents from analytical laboratory and can be used in environmental, food and fragrance, and forensic and drug analysis. This handbook offers a thorough background of the theory and practical implementation of SPME. SPME protocols are presented outlining each stage of the method and providing useful tips and potential pitfalls. In addition, devices and fiber coatings, automated SPME systems, SPME method development, and In Vivo applications are discussed. This handbook is essential for its discussion of the latest SPME developments as well as its in depth information on the history, theory, and practical application of the method. - Practical application of Solid Phase Microextraction methods including detailed steps - Provides history of extraction methods to better understand the process - Suitable for all levels, from beginning student to experienced practitioner |
| agilent gc column selection guide: Chiral Liquid Chromatography w Lough, 2012-12-06 While working as a chromatographer in the pharmaceutical industry, it became apparent to the editor that there was a pressing need for a comprehensive reference text for analysts working on the resolution of enantiomers by liquid chromatography (LC). This need arises from the fact that, whereas previously it was very difficult to determine enantiomers by direct means, there is now a wide choice of direct LC methods. At the same time, regulatory authorities have been changing their attitudes towards the administration of pharmaceuticals as racemates, partly because it is now possible to study the individual enantiomers. Clearly this abundance of new information needs to be rationalized. More importantly, the chiral LC systems which are commercially available or readily accessible to the practising chromatographer needed to be reviewed and, to a much greater extent than in existing reviews or books, discussed in terms of their practical application. Accordingly this book is very much orientated towards the practical aspects of these commercially available and readily accessible chiral LC systems. To this end, it is written for practising chromatographers by a team of practising, experienced chromatographers who have spent many years tackling the problems presented by resolving enantiomers by LC. The practical aspects of common chiral LC systems cannot be fully understood if discussed in isolation. |
| agilent gc column selection guide: A Practical Guide to the Care, Maintenance and Troubleshooting of Capillary Gas Chromatographic Systems Dean Rood, 1999-03-02 Dean Rood A Practical Guide to the Care, Maintenance, and Troubleshooting of Capillary Gas Chromatographic Systems Third, Revised Edition The field of gas chromatography continues the evolutionary process. This is well demonstrated by the continuous series of developments — in columns, equipment, apparatus, techniques, and applications — that have occured since the publication of the first edition of this very successful offering. Problems experienced by users differ from case to case, and these differences sometimes necessitate different approaches to care, maintenance, and trouble-shooting. This book is intended for the average GC user and not for those whose entire life revolves around capillary gas chromatography. The topics covered within these pages are based on the most common problems, questions, and misconceptions about capillary gas chromatography. These topics have been assembled and presented in a unique, practical, and concise format suitable even for the most inexperienced user. The author has not changed his successful approach to the topic in the present third edition. Instead, he has focused on updating and correcting the text of the widely acclaimed second edition. |
| agilent gc column selection guide: Multidimensional Chromatography Luigi Mondello, Alastair C. Lewis, Keith D. Bartle, 2002-02-15 Mehrdimensionale Chromatographie im analytischen Labor: Dieses Buch bespricht erstmals alle gängigen Verfahren sowie Anwendungen auf verschiedensten Gebieten, von der Pharmazie, Biologie und Chemie bis hin zur Umwelttechnik und erdölverarbeitender Industrie. Die Autoren sind selbst aktiv in der einschlägigen Forschung tätig. |
| agilent gc column selection guide: Gas Chromatography and Mass Spectrometry: A Practical Guide O. David Sparkman, Zelda Penton, Fulton G. Kitson, 2011-05-17 The second edition of Gas Chromatography and Mass Spectrometry: A Practical Guide follows the highly successful first edition by F.G. Kitson, B.S. Larsen, and C.N. McEwen (1996), which was designed as an indispensible resource for GC/MS practitioners regardless of whether they are a novice or well experienced. The Fundamentals section has been extensively reworked from the original edition to give more depth of an understanding of the techniques and science involved with GC/MS. Even with this expansion, the original brevity and simple didactic style has been retained. Information on chromatographic peak deconvolution has been added along with a more in-depth understanding of the use of mass spectral databases in the identification of unknowns. Since the last edition, a number of advances in GC inlet systems and sample introduction techniques have occurred, and they are included in the new edition. Other updates include a discussion on fast GC and options for combining GC detectors with mass spectrometry. The section regarding GC Conditions, Derivatization, and Mass Spectral Interpretation of Specific Compound Types has the same number of compound types as the original edition, but the information in each section has been expanded to not only explain some of the spectra but to also explain why certain fragmentations take place. The number of Appendices has been increased from 12 to 17. The Appendix on Atomic Masses and Isotope Abundances has been expanded to provide tools to aid in determination of elemental composition from isotope peak intensity ratios. An appendix with examples on Steps to follow in the determination of elemental compositions based on isotope peak intensities has been added. Appendices on whether to use GC/MS or LC/MS, third-party software for use in data analysis, list of information required in reporting GC/MS data, X+1 and X+2 peak relative intensities based on the number of atoms of carbon in an ion, and list of available EI mass spectral databases have been added. Others such as the ones on derivatization, isotope peak patterns for ions with Cl and/or Br, terms used in GC and in mass spectrometry, and tips on setting up, maintaining and troubleshooting a GC/MS system have all been expanded and updated. - Covers the practical instruction necessary for successful operation of GC/MS equipment - Reviews the latest advances in instrumentation, ionization methods, and quantitation - Includes troubleshooting techniques and a variety of additional information useful for the GC/MS practitioner - A true benchtop reference - A guide to a basic understanding of the components of a Gas Chromatograph-Mass Spectrometer (GC-MS) - Quick References to data interpretation - Ready source for information on new analyses |
Choosing the “Correct” GC Column Dimensions and ... - Agilent
Picking the appropriate stationary phase and optimum dimensions for the column will give the greatest resolution in the shortest analysis time. 2. Column Selection Page 3. Four Primary …
Agilent J&W GC Column Selection Guide - Axial Scientific
Column bleed can decrease spectral integrity, reduce uptime, and shorten column life. But Agilent J&W columns have the widest range of low-bleed standard and stationary phases featuring …
GC Column Selection Guide Cover - manuallib.com
Nov 4, 2023 · Column: DB-1,15mx0.25mmid,0.25µm Carrier: Heliumat30cm/sec Oven: 60°Cfor1min,60-180°Cat20°C/min 1. n-Decane(C10) 2. n-Undecane(C11) 3. n-Dodecane(C12) 4. …
GC Column Selection Guide - MilliporeSigma
How to Choose a Capillary Column. An optimized chromatographic separation begins with the column. The selection of the proper capillary column for any application should be based on four …
GC Column Selection Guide (420410) - PerkinElmer
This column selection guide outlines typical applications for each column so that you can be sure the chemical makeup of your sample and the chemical makeup of your column are perfectly …
Agilent GC, GC/MS, and ALS Site Preparation Guide
The specifications in this manual outline the necessary space, electrical outlets, gases, tubing, operating supplies, consumables, and other usage-dependent items such as columns, vials, …
The GC Column - Agilent
• Is it Volatile enough to chromatograph by GC? • Is it a Gas or a Liquid? • How are we getting the Sample Injected? • What is the sample Matrix? – Can we do sample clean up? • Is it an …
GC Column Selection Guide Cover
COLUMN SELECTION PRINCIPLES 5 BoilingPoint(°C) 174 196 216 234 253 268 287 Homologousseriesofhydrocarbons.Thesoluteseluteinorderoftheirincreasingboilingpoints ...
GC Column Selection Guide - market.mikro-polo.si
The selection of the proper capillary column for any application should be based on four significant factors: stationary phase, column I.D., film thickness, and column length.
Thermo Scientific GC Columns and Consumables Primer
Thermo Scientific GC consumables are available for both Thermo Scientific and Agilent instruments. Selection of the correct GC columns and accessories is critical to ensure optimum system …
More confidence in every separation - Agilent
Agilent J&W packed GC columns are designed and manufactured to offer excellent, reproducible performance for all sample types associated with packed column separations. Key advantages …
Restek Guide to GC Column Selection and Optimizing …
basics of how resolution is related to column characteristics, optimizing your analysis for both separation and speed becomes easier. This GC column selection guide discusses the basics of …
A Beginner s Guide to your GC Columns: Installation, Care, and …
Keeping your GC Column Happy and Healthy: Installation, Care, and Maintenance Agilent Restricted 28 Good installation Improper installation or injector leak Check for: • Too low of a split ratio • …
Quick Reference Guide: Agilent J&W Capillary GC columns
Maximize capillary GC column performance and lifetime by following these recommended guidelines for proper installation. Figure 1. Capillary column. Column cutting tool such as a diamond-, …
GC Column Selection Guide - Greyhound Chrom
Choosing a stationary phase is the most important step in selecting a column. A stationary phase is the film coated on the inner wall of a capillary column, and should be selected based on the …
GC Column Selection Guide - neutronco.com
GC Column Selection Guide How to Choose a Capillary Column An optimized chromatographic separation begins with the column. The selection of the proper capillary column for any …
Agilent Distributor News
“Agilent J&W GC Column Selection Guide,” publication 5989-6159EN, 144 pages, lets customers choose the highest-performing columns that deliver fast, reproducible results.
How To Select the Correct GC Column - Agilent
How To Select the Correct GC Column . Simon Jones Application Engineer . Things to Consider… • Is it Volatile enough to chromatograph by GC? • Is it a Gas or a Liquid? • How are we getting the …
The LC Handbook - Agilent
Feb 1, 2016 · compiled this handy guide to choosing LC columns, with plenty of tips and tricks to make your job easier and more productive. In addition, we’ve drawn on more than 40 years of …
Choosing the “Correct” GC Column Dimensions and ... - Agilent
Picking the appropriate stationary phase and optimum dimensions for the column will give the greatest resolution in the shortest analysis time. 2. Column Selection Page 3. Four Primary …
Agilent J&W GC Column Selection Guide - Axial Scientific
Column bleed can decrease spectral integrity, reduce uptime, and shorten column life. But Agilent J&W columns have the widest range of low-bleed standard and stationary phases featuring …
GC Column Selection Guide Cover - manuallib.com
Nov 4, 2023 · Column: DB-1,15mx0.25mmid,0.25µm Carrier: Heliumat30cm/sec Oven: 60°Cfor1min,60-180°Cat20°C/min 1. n-Decane(C10) 2. n-Undecane(C11) 3. n …
Guide to GC Column Selection and Optimizing Separations
• Learn how to choose the right column the first time. • Optimize separations for the best balance of resolution and speed. • Troubleshoot quickly and effectively based on chromatographic …
GC Column Selection Guide - MilliporeSigma
How to Choose a Capillary Column. An optimized chromatographic separation begins with the column. The selection of the proper capillary column for any application should be based on …
GC Column Selection Guide (420410) - PerkinElmer
This column selection guide outlines typical applications for each column so that you can be sure the chemical makeup of your sample and the chemical makeup of your column are perfectly …
Agilent GC, GC/MS, and ALS Site Preparation Guide
The specifications in this manual outline the necessary space, electrical outlets, gases, tubing, operating supplies, consumables, and other usage-dependent items such as columns, vials, …
The GC Column - Agilent
• Is it Volatile enough to chromatograph by GC? • Is it a Gas or a Liquid? • How are we getting the Sample Injected? • What is the sample Matrix? – Can we do sample clean up? • Is it an …
GC Column Selection Guide Cover
COLUMN SELECTION PRINCIPLES 5 BoilingPoint(°C) 174 196 216 234 253 268 287 Homologousseriesofhydrocarbons.Thesoluteseluteinorderoftheirincreasingboilingpoints ...
GC Column Selection Guide - market.mikro-polo.si
The selection of the proper capillary column for any application should be based on four significant factors: stationary phase, column I.D., film thickness, and column length.
Thermo Scientific GC Columns and Consumables Primer
Thermo Scientific GC consumables are available for both Thermo Scientific and Agilent instruments. Selection of the correct GC columns and accessories is critical to ensure …
More confidence in every separation - Agilent
Agilent J&W packed GC columns are designed and manufactured to offer excellent, reproducible performance for all sample types associated with packed column separations. Key advantages …
Restek Guide to GC Column Selection and Optimizing …
basics of how resolution is related to column characteristics, optimizing your analysis for both separation and speed becomes easier. This GC column selection guide discusses the basics …
A Beginner s Guide to your GC Columns: Installation, Care, …
Keeping your GC Column Happy and Healthy: Installation, Care, and Maintenance Agilent Restricted 28 Good installation Improper installation or injector leak Check for: • Too low of a …
Quick Reference Guide: Agilent J&W Capillary GC columns
Maximize capillary GC column performance and lifetime by following these recommended guidelines for proper installation. Figure 1. Capillary column. Column cutting tool such as a …
GC Column Selection Guide - Greyhound Chrom
Choosing a stationary phase is the most important step in selecting a column. A stationary phase is the film coated on the inner wall of a capillary column, and should be selected based on the …
GC Column Selection Guide - neutronco.com
GC Column Selection Guide How to Choose a Capillary Column An optimized chromatographic separation begins with the column. The selection of the proper capillary column for any …
Agilent Distributor News
“Agilent J&W GC Column Selection Guide,” publication 5989-6159EN, 144 pages, lets customers choose the highest-performing columns that deliver fast, reproducible results.
How To Select the Correct GC Column - Agilent
How To Select the Correct GC Column . Simon Jones Application Engineer . Things to Consider… • Is it Volatile enough to chromatograph by GC? • Is it a Gas or a Liquid? • How …
The LC Handbook - Agilent
Feb 1, 2016 · compiled this handy guide to choosing LC columns, with plenty of tips and tricks to make your job easier and more productive. In addition, we’ve drawn on more than 40 years of …
