Advertisement
Agilent 1260 Infinity Manual: A Comprehensive Guide to Best Practices and Troubleshooting
Author: Dr. Anya Sharma, PhD in Analytical Chemistry with 15 years of experience in HPLC and UHPLC techniques, including extensive use of the Agilent 1260 Infinity series.
Publisher: Chromatography Solutions, a leading publisher specializing in analytical chemistry and chromatography techniques. They offer a wide range of resources for scientists and researchers, including manuals, training materials, and troubleshooting guides.
Editor: Dr. Ben Carter, PhD in Chemical Engineering with 10 years of experience in editing scientific publications and technical manuals.
Summary: This comprehensive guide serves as an enhanced "Agilent 1260 Infinity Manual," providing detailed instructions, best practices, and troubleshooting advice for users of the Agilent 1260 Infinity series HPLC systems. It covers system setup, method development, maintenance procedures, common problems and their solutions, and data analysis, empowering users to maximize the performance and longevity of their instrument.
1. Introduction to the Agilent 1260 Infinity Manual and System Overview
The Agilent 1260 Infinity is a modular HPLC system offering high performance and versatility for a wide range of applications. This guide complements the official Agilent 1260 Infinity manual, providing practical advice and addressing common challenges encountered by users. Understanding the modularity of the system – allowing for customization with various detectors, autosamplers, and columns – is crucial for optimal performance. This "Agilent 1260 Infinity Manual" enhancement focuses on practical aspects often overlooked in standard manuals.
2. System Setup and Installation: A Step-by-Step Guide
Proper setup is paramount for accurate and reliable results. This section of our "Agilent 1260 Infinity Manual" guide details the correct installation procedures, including connecting modules, configuring software, and performing initial system checks. We emphasize the importance of following Agilent's recommended guidelines for solvent delivery, pump priming, and detector calibration. Ignoring these steps often leads to initial system errors that are easily avoidable.
3. Method Development and Optimization using the Agilent 1260 Infinity
Method development is a critical aspect of HPLC analysis. This section of our enhanced "Agilent 1260 Infinity Manual" explains the steps involved in optimizing chromatographic parameters such as mobile phase composition, flow rate, column temperature, and injection volume to achieve optimal separation and sensitivity. We will discuss gradient optimization techniques and the use of Agilent's OpenLab CDS software for method creation and management.
4. Routine Maintenance and Preventative Measures
Regular maintenance is essential for ensuring the long-term performance and reliability of your Agilent 1260 Infinity system. This part of our "Agilent 1260 Infinity Manual" guide covers preventative maintenance procedures, including flushing the system, replacing filters, and cleaning the autosampler. We highlight the importance of regular preventative maintenance to prevent costly repairs and downtime.
5. Troubleshooting Common Problems with the Agilent 1260 Infinity
This section serves as an extensive troubleshooting guide, addressing common issues encountered by Agilent 1260 Infinity users. We provide solutions for problems such as pressure fluctuations, peak tailing, ghost peaks, and detector noise. This practical section of our enhanced "Agilent 1260 Infinity Manual" significantly reduces troubleshooting time and frustration.
6. Data Analysis and Reporting
Understanding the data generated by the Agilent 1260 Infinity is crucial. This section focuses on data processing using OpenLab CDS software, including peak integration, quantification, and report generation. We discuss various data analysis techniques and best practices for ensuring data integrity. Efficient data analysis is critical for accurate interpretation of results.
7. Advanced Techniques and Applications
This section explores advanced applications of the Agilent 1260 Infinity, such as method validation, system suitability testing, and quality control. It delves into specialized techniques applicable to diverse analytical challenges, expanding upon the basic functionalities outlined in the standard Agilent 1260 Infinity manual.
8. Safety Precautions and Regulatory Compliance
Working with HPLC systems requires adherence to safety protocols. This section emphasizes safety procedures related to handling solvents, high-pressure systems, and electrical components. We also address regulatory compliance aspects, ensuring users understand relevant safety regulations and standards.
9. Conclusion
The Agilent 1260 Infinity is a powerful and versatile HPLC system. This comprehensive guide, built upon the standard Agilent 1260 Infinity manual, provides the practical knowledge and troubleshooting skills necessary to maximize the instrument’s potential. By following the best practices and preventative maintenance outlined herein, users can ensure accurate, reliable results and prolong the lifespan of their system.
FAQs
1. How often should I perform preventative maintenance on my Agilent 1260 Infinity? Follow Agilent's recommendations, but generally, weekly checks and monthly more in-depth maintenance are advised.
2. What are the most common causes of pressure fluctuations in the Agilent 1260 Infinity? Air bubbles in the system, blocked filters, or column issues are frequently the culprits.
3. How do I troubleshoot ghost peaks in my chromatograms? System contamination is often the cause; thorough system cleaning and mobile phase purity checks are crucial.
4. What is the best way to optimize my gradient method for the Agilent 1260 Infinity? Use Agilent's OpenLab software and systematically vary parameters like gradient slope and solvent composition.
5. How can I improve peak shape in my Agilent 1260 Infinity analysis? Check column condition, ensure proper equilibration, and optimize mobile phase pH.
6. How do I calibrate the detector on my Agilent 1260 Infinity? Follow the instructions provided in the Agilent 1260 Infinity manual and use certified standards.
7. What are the common causes of detector noise in the Agilent 1260 Infinity? Electrical interference, improper grounding, or worn-out detector components are possible causes.
8. How do I troubleshoot a pump failure in my Agilent 1260 Infinity system? Check for air bubbles, leaks, and ensure proper priming; contact Agilent support if needed.
9. Where can I find additional resources and support for my Agilent 1260 Infinity? Visit Agilent's website for manuals, troubleshooting guides, and contact information for technical support.
Related Articles:
1. Agilent 1260 Infinity Troubleshooting Guide: Pressure Issues: Focuses specifically on diagnosing and resolving pressure-related problems in the Agilent 1260 Infinity HPLC.
2. Optimizing Gradient Elution on the Agilent 1260 Infinity: A detailed guide to method development and optimization using gradient techniques on the Agilent 1260 Infinity.
3. Agilent 1260 Infinity Maintenance Checklist: A comprehensive checklist for regular preventative maintenance of the Agilent 1260 Infinity.
4. Data Analysis Techniques for Agilent 1260 Infinity Data: Covers advanced data processing techniques using OpenLab CDS software for Agilent 1260 Infinity data.
5. Agilent 1260 Infinity and OpenLab CDS Integration: A practical guide on integrating the Agilent 1260 Infinity with OpenLab CDS software for seamless data acquisition and analysis.
6. Common Errors and Solutions for Agilent 1260 Infinity Autosampler: Focuses on troubleshooting issues related to the autosampler module of the Agilent 1260 Infinity.
7. Validating HPLC Methods on the Agilent 1260 Infinity: Discusses the process of validating HPLC methods according to regulatory guidelines using the Agilent 1260 Infinity.
8. Advanced Applications of the Agilent 1260 Infinity in Pharmaceutical Analysis: Explores the use of the Agilent 1260 Infinity in pharmaceutical analysis, including impurity analysis and quality control.
9. Comparative Study: Agilent 1260 Infinity vs. Other HPLC Systems: Compares the Agilent 1260 Infinity with other leading HPLC systems, highlighting its strengths and weaknesses.
| agilent 1260 infinity manual: Preparative Liquid Chromatography B.A. Bidlingmeyer, 1987-07-01 This volume provides a straightforward approach to isolation and purification problems with a thorough presentation of preparative LC strategy including the interrelationship between the input and output of the instrumentation, while keeping to an application focus.The book stresses the practical aspects of preparative scale separations from TLC isolations through various laboratory scale column separations to very large scale production. It also gives a thorough description of the performance parameters (e.g. throughput, separation quality, etc.) as a function of operational parameters (e.g. particle size, column size, solvent usage, etc.). Experts in the field have contributed a well balanced presentation of separation development strategies from preparative TLC to commercial preparative process with practical examples in a wide variety of application areas such as drugs, proteins, nucleotides, industrial extracts, organic chemicals, enantiomers, polymers, etc. |
| agilent 1260 infinity manual: Optimization in HPLC Stavros Kromidas, 2021-08-06 Learn to maximize the performance of your HPLC or UHPLC system with this resource from leading experts in the field Optimization in HPLC: Concepts and Strategies delivers tried-and-tested strategies for optimizing the performance of HPLC and UHPLC systems for a wide variety of analytical tasks. The book explains how to optimize the different HPLC operation modes for a range of analyses, including small molecules, chiral substances, and biomolecules. It also shows readers when and how computational tools may be used to optimize performance. The practice-oriented text describes common challenges faced by users and developers of HPLC and UHPLC systems, as well as how those challenges can be overcome. Written for first-time and experienced users of HPLC technology and keeping pace with recent developments in HPLC instrumentation and operation modes, this comprehensive guide leaves few questions unanswered. Readers will also benefit from the inclusion of: A thorough introduction to optimization strategies for different modes and uses of HPLC, including working under regulatory constraints An exploration of computer aided HPLC optimization, including ChromSwordAuto and Fusion QbD A treatment of current challenges for HPLC users in industry as well as large and small analytical service providers Discussions of current challenges for HPLC equipment suppliers Tailor-made for analytical chemists, chromatographers, pharmacologists, toxicologists, and lab technicians, Optimization in HPLC: Concepts and Strategies will also earn a place on the shelves of analytical laboratories in academia and industry who seek a one-stop reference for optimizing the performance of HPLC systems. |
| agilent 1260 infinity manual: Liquid Chromatography/Mass Spectrometry, MS/MS and Time of Flight MS Imma Ferrer, E. M. Thurman, 2003-08-14 This volume explores state-of-the-art mass spectrometric techniques. It focuses on liquid chromatography/mass spectrometry/mass spectrometry and time-of-flight/mass spectrometry to determine emerging contaminants, such as pharmaceuticals, hormones, pesticides, surfactants and unknown natural products. |
| agilent 1260 infinity manual: The HPLC Expert II Stavros Kromidas, 2017-01-05 How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful HPLC Expert in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications. |
| agilent 1260 infinity manual: Practical HPLC Methodology and Applications Brian A. Bidlingmeyer, 1993-05-06 Of related interest. Trace and Ultratrace Analysis by HPLC Satinder Ahuja Written by a leading scientist in the field, this monograph provides the first definitive and technically up-to-date treatment of the theory, equipment, and applications of chemistry's most powerful reliable analytical technique. Coverage includes an encyclopedic compendium of common substances that require trace and ultratrace analysis, and features clear discussion of such important topics as considerations for HPLC equipment, sensitive detectors, sample preparation, method development, selectivity and computer-based optimizations, optimizing detectability, and much more. 1991 (0 471-51419-5) 432 pp. High Performance Liquid Chromatography in Biotechnology Edited by William S. Hancock Analytical chemists, biochemists, and chemical engineers will find this up-to-date guide to HPLC's recent developments essential for enhancing on-the-job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as those in monoclonal antibody and nucleic acid purification. Techniques for quality control programs and advanced technology are also discussed. 1990 (0 471-82584-0) 564 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together for the first time the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physical-mathematical form, facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide background material applicable to a wide range of separation methods, while the final five chapters illustrate specific techniques and methods. 1991 (0 471-52089-6) 320 pp. |
| agilent 1260 infinity manual: Optimization in HPLC Stavros Kromidas, 2021-11-08 Learn to maximize the performance of your HPLC or UHPLC system with this resource from leading experts in the field Optimization in HPLC: Concepts and Strategies delivers tried-and-tested strategies for optimizing the performance of HPLC and UHPLC systems for a wide variety of analytical tasks. The book explains how to optimize the different HPLC operation modes for a range of analyses, including small molecules, chiral substances, and biomolecules. It also shows readers when and how computational tools may be used to optimize performance. The practice-oriented text describes common challenges faced by users and developers of HPLC and UHPLC systems, as well as how those challenges can be overcome. Written for first-time and experienced users of HPLC technology and keeping pace with recent developments in HPLC instrumentation and operation modes, this comprehensive guide leaves few questions unanswered. Readers will also benefit from the inclusion of: A thorough introduction to optimization strategies for different modes and uses of HPLC, including working under regulatory constraints An exploration of computer aided HPLC optimization, including ChromSwordAuto and Fusion QbD A treatment of current challenges for HPLC users in industry as well as large and small analytical service providers Discussions of current challenges for HPLC equipment suppliers Tailor-made for analytical chemists, chromatographers, pharmacologists, toxicologists, and lab technicians, Optimization in HPLC: Concepts and Strategies will also earn a place on the shelves of analytical laboratories in academia and industry who seek a one-stop reference for optimizing the performance of HPLC systems. |
| agilent 1260 infinity manual: The Bios Companion Phil Croucher, 2001 This text describes the functions that the BIOS controls and how these relate to the hardware in a PC. It covers the CMOS and chipset set-up options found in most common modern BIOSs. It also features tables listing error codes needed to troubleshoot problems caused by the BIOS. |
| agilent 1260 infinity manual: Supercritical Fluid Chromatography Gérard Rossé, 2018-12-17 Supercritical Fluid Chromatography (SFC) provides a timely overview of SFC application areas which were unimaginable just a decade ago. This two-volume series opens with an overview of the history and expectant future of SFC and continues with recent applications in the pharmaceutical industry and other fascinating areas of science. SFC has found its place in the pharmaceutical industry with an increasing body of applications for chiral and achiral molecules in both the research and development phases of the drug discovery process. As illustrated in this two-volume series, the current interest in SFC extends well beyond the pharmaceutical industry. Chapters encompassing applications for polar and non-polar mixtures of importance are covering widely disparate areas in substance abuse, natural products including cannabinoids, bioactive lipids, flavor and fragrance. With its broad balance and coverage, this two-volume book constitutes a unique educational platform to students and scientists for many years to come. The major objective of this book editions is to inspire and stimulate readers to continue exploring the possibilities of exploiting supercritical fluids as a particular media for analysis, purifications and synthesis |
| agilent 1260 infinity manual: High Performance Liquid Chromatography & Capillary Electrophoresis Andrea Weston, Phyllis R. Brown, 1997-07-21 HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity.Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. - Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques - Applicable to a wide variety of separation problems - Covers basic concepts governing any separation as well as instrumentation and how to use it - Helps the user to obtain optimal resolution in minimal time - Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation - Includes information on upcoming trends such as miniaturization - Major concepts in each chapter are organized to allow access to information easily and quickly - Contains practical bibliography for accessing the literature |
| agilent 1260 infinity manual: HPLC and UHPLC for Practicing Scientists Michael W. Dong, 2019-07-23 A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher. |
| agilent 1260 infinity manual: Mixture Toxicity Cornelis A. M. van Gestel, Martijs Jonker, Jan E. Kammenga, Ryszard Laskowski, Claus Svendsen, 2016-04-19 In the last decade and a half, great progress has been made in the development of concepts and models for mixture toxicity, both in human and environmental toxicology. However, due to their different protection goals, developments have often progressed in parallel but with little integration. Arguably the first book to clearly link ecotoxicology an |
| agilent 1260 infinity manual: INCREaSE António Mortal, Jaime Aníbal, Jânio Monteiro, Cláudia Sequeira, Jorge Semião, Manuela Moreira da Silva, Miguel Oliveira, 2018-01-04 This book presents the proceedings of the INternational CongRess on Engineering and Sustainability in the XXI cEntury – INCREaSE 2017, which was held in Faro, Portugal, from October 11 to 13, 2017. The book promotes a multidisciplinary approach to sustainable development, exploring a number of transversal challenges. It discusses natural and anthropogenic risks; tourism and sustainability; healthy food; water and society; sustainable mobility; renewable energy; and energy efficiency, offering perspectives from civil, electronics, mechanical and food engineering. |
| agilent 1260 infinity manual: ICIPEG 2014 Mariyamni Awang, Berihun Mamo Negash, Nur Asyraf Md Akhir, Luluan Almanna Lubis, 2015-03-20 This book presents the proceedings of the 3rd International Conference on Integrated Petroleum Engineering and Geosciences 2014 (ICIPEG2014). Topics covered on the petroleum engineering side include reservoir modeling and simulation, enhanced oil recovery, unconventional oil and gas reservoirs, production and operation. Similarly geoscience presentations cover diverse areas in geology, geophysics palaeontology and geochemistry. The selected papers focus on current interests in petroleum engineering and geoscience. This book will be a bridge between engineers, geoscientists, academicians and industry. |
| agilent 1260 infinity manual: Electronic Portable Instruments Halit Eren, 2003-10-16 With the availability of advanced technologies, digital systems, and communications, portable instruments are rapidly evolving from simple, stand alone, low-accuracy measuring instruments to complex multifunctional, network integrated, high-performance digital devices with advanced interface capabilities. The relatively brief treatments these instr |
| agilent 1260 infinity manual: Pll Performance, Simulation and Design Dean Banerjee, 2006-08 This book is intended for the reader who wishes to gain a solid understanding of Phase Locked Loop architectures and their applications. It provides a unique balance between both theoretical perspectives and practical design trade-offs. Engineers faced with real world design problems will find this book to be a valuable reference providing example implementations, the underlying equations that describe synthesizer behavior, and measured results that will improve confidence that the equations are a reliable predictor of system behavior. New material in the Fourth Edition includes partially integrated loop filter implementations, voltage controlled oscillators, and modulation using the PLL. |
| agilent 1260 infinity manual: UHPLC in Life Sciences Davy Guillarme, Jean-Luc Veuthey, 2015-11-09 Since its commercial introduction in 2004, UHPLC (Ultra-High Performance Liquid Chromatography) has begun to replace conventional HPLC in academia and industry and interest in this technique continues to grow. Both the increases in speed and resolution make this an attractive method; particularly to the life sciences and more than 1500 papers have been written on this strongly-evolving topic to date. This book provides a solid background on how to work with UHPLC and its application to the life sciences. The first part of the book covers the basics of this approach and the specifics of a UHPLC system, providing the reader with a solid background to working properly with such a system. The second part examines the application of UHPLC to the life sciences, with a focus on drug analysis strategies. UHPLC-MS, a key technique in pharmaceutical and toxicological analyses, is also examined in detail. The editors (Davy Guillarme and Jean-Luc Veuthey) were some of the earliest adopters of UHPLC and have published and lectured extensively on this topic. Between them they have brought together an excellent team of contributors from Europe and the United States, presenting a wealth of expertise and knowledge. This book is an essential handbook for anyone wishing to adopt an UHPLC system in either an academic or industrial setting and will benefit postgraduate students and experienced workers alike. |
| agilent 1260 infinity manual: Applied Biocatalysis John Whittall, Peter W. Sutton, 2020-08-21 Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistry This book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and includes short but detailed experimental methods on timely novel biocatalytic transformations using new enzymes or new methodologies using known enzymes. The book also features reactions that are “expanding and making the enzyme toolbox available to chemists”—providing readers with comprehensive methodology and detailed key sourcing information of a wide range of enzymes. Chapters in Applied Biocatalysis: The Chemist’s Enzyme Toolkit are organized by reaction type and feature a short introductory section describing the current state of the art for each example. Much of the book focuses on processes for which the enzymes are readily available so that organic chemists can synthesize appropriate quantities of chemicals with available materials in a standard chemical laboratory. Advanced methods are included to present examples of new enzymes that might encourage collaboration with suppliers or academic groups and that will educate chemists of rapidly expanding future possibilities. Focuses on current topics within the applied industrial biocatalysis field Offers experimental methods on novel biocatalytic transformations using new enzymes or new methodology using known enzymes Covers the hot topics of enzyme and chemoenzymatic cascades and biocatalysis in flow Edited by noted experts from both academia and industry with years of experience in the field of biocatalysis—particularly, the industrial applications of enzymes Written for synthetic organic chemists working in all industries but especially the pharmaceutical industry and for those in academia with an eye for biocatalysis, Applied Biocatalysis: The Chemist’s Enzyme Toolkit will also benefit academic groups in chemistry and related sciences that are using enzymes for synthetic purposes, as well as those working in the area of enzymology and molecular biology. |
| agilent 1260 infinity manual: Mass Spectrometry for the Clinical Laboratory Hari Nair, William Clarke, 2016-11-02 Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to meet CLIA requirements, appropriate interference testing, measuring, validation of ion suppression/matrix effects, and quality control. These tools offer guidance on what type of instrumentation is optimal for each assay, what options are available, and the pros and cons of each. Readers will find a full set of tools that are either directly related to the assay they want to adopt or for an analogous assay they could use as an example. Written by expert users of the most common assays found in a clinical laboratory (clinical chemists, toxicologists, and clinical pathologists practicing mass spectrometry), the book lays out how experts in the field have chosen their mass spectrometers, purchased, installed, validated, and brought them on line for routine testing. The early chapters of the book covers what the practitioners have learned from years of experience, the challenges they have faced, and their recommendations on how to build and validate assays to avoid problems. These chapters also include recommendations for maintaining continuity of quality in testing. The later parts of the book focuses on specific types of assays (therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has been written by an expert practitioner of an assay that is currently running in his or her clinical lab. Provides readers with the keys to choosing, installing, and validating a mass spectrometry platform Offers tools to evaluate, validate, and troubleshoot the most common assays seen in clinical pathology labs Explains validation, ion suppression, interference testing, and quality control design to the detail that is required for implementation in the lab |
| agilent 1260 infinity manual: Mass Spectrometry-Based Lipidomics Fong-Fu Hsu, 2021-05-29 This detailed volume covers conventional MS-based “shotgun lipidomics” by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies. |
| agilent 1260 infinity manual: Food Analysis by HPLC Leo M.L. Nollet, Fidel Toldra, 2012-11-16 For food scientists, high-performance liquid chromatography (HPLC) is a powerful tool for product composition testing and assuring product quality. Since the last edition of this volume was published, great strides have been made in HPLC analysis techniques-with particular attention given to miniaturization, automatization, and green chemistry. Tho |
| agilent 1260 infinity manual: Complete Wireless Design Cotter Sayre, 2001-02-12 Easily design today’s wireless systems and circuits Design an entire radio system from the ground up instead of relying on a simple plug-in selection of circuits to be modified. Avoid an arduous trek through theory and mathematical derivations. Cotter Sayre’s Complete Wireless Design covers wireless hardware design more thoroughly than any other handbook —and does it without burying you in math. This new guide from today’s bestselling wireless author gives you all the skills you need to design wireless systems and circuits. If you want to climb the learning curve with grace, and start designing what you need immediately, this reasonably priced resource is your best choice. It’s certain to be the most-used reference in your wireless arsenal for designing cutting-edge filters, amplifiers, RF switches, oscillators, and more. You get: Simplified calculations for impedance matching, analysis of wireless links, and completing a frequency plan Real-world examples of designing with RFIC’s and MMIC’s Full circuit and electromagnetic software simulations More |
| agilent 1260 infinity manual: Packed Column SFC T A Berger, 2007-10-31 Packed Column SFC is the third title to be published in this series and has been produced as a result of the dramatic re-emergence, in the last three years, of packed column instrumentation. This has led to a redefinition of the technique and an urgent need for a practical guide that deals with its subtleties. This book fulfils that need and deals exclusively with packed column SFC. It places the emphasis on understanding the underlying chemistry in order to perform rapid, systematic optimizations and provides many practical tips to help the new user avoid problems unique to SFC. It also proposes a detailed scheme for method development and provides lists of prioritized guidelines. The book clears up some of the confusion that surrounds the analytical use of supercritical fluids and assists the user in understanding the power and utility of this technique. Detailed chapters cover the most promising new application areas for packed column SFC, which are often overlooked in the mainstream chromatography literature. Like the other books in this popular series, Packed Column SFC will prove an invaluable guide and is essential reading for graduates, postgraduates and researchers with interests in pharmaceuticals, agricultural chemistry, small polar drug molecules, chiral analysis, environmental chemistry, and chromatography/instrumentation. |
| agilent 1260 infinity manual: Handbook of Pharmaceutical Analysis by HPLC Satinder Ahuja, Michael Dong, 2005-02-09 High pressure liquid chromatography–frequently called high performance liquid chromatography (HPLC or, LC) is the premier analytical technique in pharmaceutical analysis and is predominantly used in the pharmaceutical industry. Written by selected experts in their respective fields, the Handbook of Pharmaceutical Analysis by HPLC Volume 6, provides a complete yet concise reference guide for utilizing the versatility of HPLC in drug development and quality control. Highlighting novel approaches in HPLC and the latest developments in hyphenated techniques, the book captures the essence of major pharmaceutical applications (assays, stability testing, impurity testing, dissolution testing, cleaning validation, high-throughput screening). A complete reference guide to HPLC Describes best practices in HPLC and offers 'tricks of the trade' in HPLC operation and method development Reviews key HPLC pharmaceutical applications and highlights currents trends in HPLC ancillary techniques, sample preparations, and data handling |
| agilent 1260 infinity manual: LC-MS in Drug Analysis Loralie J. Langman, Christine L. H. Snozek, 2012-07-06 Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance. The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques. The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies. |
| agilent 1260 infinity manual: Smart Cameras Ahmed Nabil Belbachir, 2009-10-20 A smart camera is an integrated machine vision system which, in addition to image capture circuitry, includes a processor, which can extract information from images without need for an external processing unit, and interface devices used to make results available to other devices. This book provides content on smart cameras for an interdisciplinary audience of professionals and students in embedded systems, image processing, and camera technology. It serves as a self-contained, single-source reference for material otherwise found only in sources such as conference proceedings, journal articles, or product data sheets. Coverage includes the 50 year chronology of smart cameras, their technical evolution, the state-of-the art, and numerous applications, such as surveillance and monitoring, robotics, and transportation. |
| agilent 1260 infinity manual: HPLC in Food Analysis R. Macrae, 1988-01 Theory and practice of HPLC; Applications of HPLC to food analysis; Determination of carborydrates; The analysis of lipids by HPLC; Determination of vitamins; Determination of food additives by HPLC; Determination of synthetic food colours by HPLC; HPLC of natural pigments in foodstuffs; Determination of mycotoxins; Determination of polynuclear aromatic hydrocarbons and nitrosamines; Determination of pesticide residues; Determination of amino acids; liquid chromatography/mass spectrometry. |
| agilent 1260 infinity manual: Analog Circuit Design Volume 2 Bob Dobkin, Jim Williams, 2012-12-31 Analog circuit and system design today is more essential than ever before. With the growth of digital systems, wireless communications, complex industrial and automotive systems, designers are being challenged to develop sophisticated analog solutions. This comprehensive source book of circuit design solutions aids engineers with elegant and practical design techniques that focus on common analog challenges. The book's in-depth application examples provide insight into circuit design and application solutions that you can apply in today's demanding designs. - This is the companion volume to the successful Analog Circuit Design: A Tutorial Guide to Applications and Solutions (October 2011), which has sold over 5000 copies in its the first 6 months of since publication. It extends the Linear Technology collection of application notes, which provides analog experts with a full collection of reference designs and problem solving insights to apply to their own engineering challenges - Full support package including online resources (LTSpice) - Contents include more application notes on power management, and data conversion and signal conditioning circuit solutions, plus an invaluable circuit collection of reference designs |
| agilent 1260 infinity manual: Laboratory Methods in Microfluidics Basant Giri, 2017-05-15 Laboratory Methods in Microfluidics features a range of lab methods and techniques necessary to fully understand microfluidic technology applications. Microfluidics deals with the manipulation of small volumes of fluids at sub-millimeter scale domain channels. This exciting new field is becoming an increasingly popular subject both for research and education in various disciplines of science, including chemistry, chemical engineering and environmental science. The unique properties of microfluidic technologies, such as rapid sample processing and precise control of fluids in assay have made them attractive candidates to replace traditional experimental approaches. Practical for students, instructors, and researchers, this book provides a much-needed, comprehensive new laboratory reference in this rapidly growing and exciting new field of research. - Provides a number of detailed methods and instructions for experiments in microfluidics - Features an appendix that highlights several standard laboratory techniques, including reagent preparation plus a list of materials vendors for quick reference - Authored by a microfluidics expert with nearly a decade of research on the subject |
| agilent 1260 infinity manual: Introduction to Modern Liquid Chromatography Lloyd R. Snyder, Joseph J. Kirkland, John W. Dolan, 2011-09-20 The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the heart of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available. |
| agilent 1260 infinity manual: Lipidomics Xianlin Han, 2016-05-02 Covers the area of lipidomics from fundamentals and theory to applications Presents a balanced discussion of the fundamentals, theory, experimental methods and applications of lipidomics Covers different characterizations of lipids including Glycerophospholipids; Sphingolipids; Glycerolipids and Glycolipids; and Fatty Acids and Modified Fatty Acids Includes a section on quantification of Lipids in Lipidomics such as sample preparation; factors affecting accurate quantification; and data processing and interpretation Details applications of Lipidomics Tools including for Health and Disease; Plant Lipidomics; and Lipidomics on Cellular Membranes |
| agilent 1260 infinity manual: The HPLC Expert II Stavros Kromidas, 2017-01-05 How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful HPLC Expert in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications. |
| agilent 1260 infinity manual: Advances in the Use of Liquid Chromatography Mass Spectrometry (LC-MS): Instrumentation Developments and Applications , 2018-01-02 Advances in the Use of Liquid Chromatography Mass Spectrometry (LC-MS): Instrumentation Developments and Application, Volume 79, highlights the most recent LC-MS evolutions through a series of contributions by world renowned scientists that will lead the readers through the most recent innovations in the field and their possible applications. Many authoritative books on LC-MS are already present in market, describing in detail the different interfaces and their principles of operation. This book focuses more on new trends, starting with the innovations of each technique, to the most progressive challenges of LC-MS. - Presents an understanding of the new advancements in LC and MS which are essential for a step forward in LC-MS applications - Provides insight into the state-of-the-art in the currently available LC-MS interfaces and their principle of use - Expounds on the new frontiers in LC-MS and their application potential |
| agilent 1260 infinity manual: High-Performance Gradient Elution Lloyd R. Snyder, John W. Dolan, 2007-01-09 Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool. |
| agilent 1260 infinity manual: Gnotobiotics Trenton R Schoeb, Kathryn A Eaton, 2017-08-11 Gnotobiotics summarizes and analyzes the research conducted on the use of gnotobiotes, providing detailed information regarding actual facility operation and derivation of gnotobiotic animals. In response to the development of new tools for microbiota and microbiome analysis, the increasing recognition of the various roles of microbiota in health and disease, and the consequent expanding demand for gnotobiotic animals for microbiota/microbiome related research, this volume collates the research of this expanding field into one definitive resource. - Reviews and defines gnotobiotic animal species - Analyzes microbiota in numerous contexts - Presents detailed coverage of the protocols and operation of a gnotobiotic facility |
| agilent 1260 infinity manual: Proteomics Lucio Comai, Jonathan E. Katz, Parag Mallick, 2017-02-11 This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field. |
| agilent 1260 infinity manual: Vitamins In Foods George F.M. Ball, 2005-11-01 To achieve and maintain optimal health, it is essential that the vitamins in foods are present in sufficient quantity and are in a form that the body can assimilate. Vitamins inFoods: Analysis, Bioavailability, and Stability presents the latest information about vitamins and their analysis, bioavailability, and stability in foods. |
| agilent 1260 infinity manual: Epidemiology of Communicable and Non-Communicable Diseases Fyson Kasenga, 2016-11-09 Human sufferings, including deaths, can be reduced or avoided by applying routine principles of hygiene in individuals' lives. Some hygiene routines are purely simple remedies, which are inexpensive, affordable, acceptable and easily accessible. It is evident that change is first enacted from within the mindset of an individual, then transmitted to families, groups and communities, and eventually the mindset of a nation can change creating an environment which is better for everybody to live in. This book contains chapters discussing conditions or diseases that may not be common in the readers' area. Caution as such may never be underestimated considering the fact that we are living in a global village where one can never say 'this does not occur in my area' but rather question, does this occur in my community, why does it occur, who is affected, where and when does it occur and what can be done about it? These questions constitute what epidemiology is all about, and their precise and comprehensive answers can transform lives and help us have the right perceptions for the health challenges we face and accept the possibility of dealing with them directly. |
| agilent 1260 infinity manual: The Principles of Nuclear Magnetism A. Abragam, 1961 The Principles of Nuclear Magnetism |
| agilent 1260 infinity manual: Cytochrome P450 Zhengyin Yan, Gary W. Caldwell, 2021 This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. Authoritative and practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines. |
| agilent 1260 infinity manual: Solid-Phase Microextraction Constantinos K. Zacharis, Paraskevas D. Tzanavaras, 2020-02-07 This book covers the most recent research activities and achievements regarding to the solid phase microextraction (SPME) technique. It is a powerful sample preparation tool that addresses the new challenges of analytical laboratories. Among others, its fundamental applications involved the sampling of volatile compounds from various matrixes. The demonstrated topics ranged from aroma characterization of various fruits, essential oils to the utilization of SPME for in-tube extraction and isolation of selected compounds from complex samples followed by state-of-the-art analytical techniques. |
Capillary Column Installation Quick Reference Guide - Agilent …
Nov 10, 2022 · This Information Applies To: Agilent GC Systems Issue The data rate determines the number of sampling points across the chromatographic peak and the sample background. …
General Recommendations for Using an Agilent RID
Nov 10, 2022 · All Agilent degassers can be used except for G1379A and G1379B. Always have a thermostatted column compartment (TCC) / multicolumn thermostat (MCT) in your stack. …
4150 and 4200 TapeStation - Wiki - Automated Electrophoresis
Here is a collection of Agilent 4150 and 4200 TapeStation Resources. Links to user manuals , application resources and technical notes , quick guides , software
How to Bake-out the Split/Splitless Inlet (S/SL) on Agilent GC …
Nov 10, 2022 · This Information Applies To: Agilent 7697A headspace sampler. Issue: The headspace sampler may present a flow shutdown or poor chromatography with decreased …
How to Check, Configure and Use the IP Address of an Agilent …
Nov 10, 2022 · Agilent HPLC modules can be configured to use either a default IP address (192.168.254.11) that cannot be changed (static), or a stored IP address that can be defined by …
Recommended Column Nut and Ferrule Combinations for GC …
Jul 17, 2024 · This Information Applies To: Agilent 6850, 6890, 7820, 7890, 8850, 8860, 8890, Intuvo 9000 GC Systems with a Thermal Conductivity Detector (TCD) Issue The TCD …
Agilent Community
Seahorse Analytics - the newest data analysis application for the Agilent Seahorse XF Analzyer Products. Seahorse XF Assay Learning Center - a learning center designed to introduce you …
Collection of 7000 and 7010 Triple Quadrupole GC/MS Resources
This collection of Agilent triple quadrupole (TQ) GC/MS resources includes l inks to user manuals, reference guides, application notes and videos for the 7000 Series Triple Quadrupole GC/MS …
Collection of 8890, 8860, 8850, Intuvo 9000, 990 ... - Agilent …
This is a collection of popular Agilent GC resources, including user manuals, application notes, troubleshooting information, tools, and supplies on Agilent.com. Go to Collection of GC/MS …
Setting up and Running the MassHunter Optimizer Tool
Oct 13, 2023 · This information applies to: Agilent 6400 Triple Quadrupole LC/MS systems, MassHunter Workstation 10.1 or earlier. Issue The MassHunter Optimizer tool allows users to …
Capillary Column Installation Quick Reference Guide - Agilent …
Nov 10, 2022 · This Information Applies To: Agilent GC Systems Issue The data rate determines the number of sampling points across the chromatographic peak and the sample background. …
General Recommendations for Using an Agilent RID
Nov 10, 2022 · All Agilent degassers can be used except for G1379A and G1379B. Always have a thermostatted column compartment (TCC) / multicolumn thermostat (MCT) in your stack. …
4150 and 4200 TapeStation - Wiki - Automated Electrophoresis
Here is a collection of Agilent 4150 and 4200 TapeStation Resources. Links to user manuals , application resources and technical notes , quick guides , software
How to Bake-out the Split/Splitless Inlet (S/SL) on Agilent GC …
Nov 10, 2022 · This Information Applies To: Agilent 7697A headspace sampler. Issue: The headspace sampler may present a flow shutdown or poor chromatography with decreased …
How to Check, Configure and Use the IP Address of an Agilent LC …
Nov 10, 2022 · Agilent HPLC modules can be configured to use either a default IP address (192.168.254.11) that cannot be changed (static), or a stored IP address that can be defined …
Recommended Column Nut and Ferrule Combinations for GC and …
Jul 17, 2024 · This Information Applies To: Agilent 6850, 6890, 7820, 7890, 8850, 8860, 8890, Intuvo 9000 GC Systems with a Thermal Conductivity Detector (TCD) Issue The TCD …
Agilent Community
Seahorse Analytics - the newest data analysis application for the Agilent Seahorse XF Analzyer Products. Seahorse XF Assay Learning Center - a learning center designed to introduce you …
Collection of 7000 and 7010 Triple Quadrupole GC/MS Resources
This collection of Agilent triple quadrupole (TQ) GC/MS resources includes l inks to user manuals, reference guides, application notes and videos for the 7000 Series Triple Quadrupole GC/MS …
Collection of 8890, 8860, 8850, Intuvo 9000, 990 ... - Agilent …
This is a collection of popular Agilent GC resources, including user manuals, application notes, troubleshooting information, tools, and supplies on Agilent.com. Go to Collection of GC/MS …
Setting up and Running the MassHunter Optimizer Tool
Oct 13, 2023 · This information applies to: Agilent 6400 Triple Quadrupole LC/MS systems, MassHunter Workstation 10.1 or earlier. Issue The MassHunter Optimizer tool allows users to …
