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| destain solution for coomassie: Gel Electrophoresis of Proteins B. D. Hames, 1998-10-01 This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods were presented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so. |
| destain solution for coomassie: Guide to Protein Purification Murray P. Deutscher, 1990 Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure. |
| destain solution for coomassie: Protein Gel Detection and Imaging Biji T. Kurien, R. Hal Scofield, 2019-08-24 This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field. |
| destain solution for coomassie: Principles and Reactions of Protein Extraction, Purification, and Characterization Hafiz Ahmed, Hafiz Ahmed PhD, 2017-07-27 Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different |
| destain solution for coomassie: Protein Electrophoresis Biji T. Kurien, R. Hal Scofield, 2012-05-16 Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field. |
| destain solution for coomassie: The Protein Protocols Handbook John M. Walker, 2008-02-12 In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose. |
| destain solution for coomassie: Quantitative Proteomics by Mass Spectrometry Salvatore Sechi, 2008-02-05 This volume is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral. Attention is given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. This volume is an indispensable resource in the search for novel biomarkers. |
| destain solution for coomassie: Principles and Techniques of Biochemistry and Molecular Biology Keith Wilson, John Walker, 2010-03-04 Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry. |
| destain solution for coomassie: Protein Sequencing and Identification Using Tandem Mass Spectrometry Michael Kinter, Nicholas E. Sherman, 2005-04-12 How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more |
| destain solution for coomassie: Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition Leland J. Cseke, Peter B. Kaufman, Gopi K. Podila, Chung-Jui Tsai, 2003-11-24 Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology. |
| destain solution for coomassie: Calpain Methods and Protocols John S. Elce, 2008-02-05 The purpose of Calpain Methods and Protocols is quite straightf- ward: it is to present the actual experimental methods used in many different laboratories for the study of calpain. It will provide the vital experimental detail, and the discussion of possible pitfalls, for which the standard journals no longer provide space. This will make it as easy as possible for investi- tors interested in calpain to adopt established methods without repeating old mistakes, and to adapt and apply these methods in novel approaches to the many outstanding calpain questions. These questions range from purely biochemical problems of protein structure and enzyme regulation at the molecular level, through large areas of cell biology, to applied and clinical aspects of calpain function in human d- ease. Within this panoply of topics, a wide range of investigators will find many fascinating and as yet unanswered questions about calpain. Calpain Methods and Protocols will provide instant access to many essential te- niques, while saving them the time and effort involved in developing a new method. In addition to questions relating to the normal physiological roles of the calpains, there is considerable evidence that inappropriate calpain activity may have pathological effects in many tissues, for example, following ischemia. This provides a major stimulus for the development of specific calpain inhi- tors for therapeutic purposes, and for the development of methods to evaluate such inhibitors. |
| destain solution for coomassie: Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls John T. Corthell, 2014-04-16 Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. - Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding - Offers many details not present in other protocol books - Describes relevant controls for each technique and what those controls mean - Chapters include references (key articles, books, protocols) for additional study - Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules |
| destain solution for coomassie: The Proteome Revisited A. Stoyanov, M. Zhukov, Pier Giorgio Righetti, 2001-10-25 The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time. |
| destain solution for coomassie: Biomarker Methods in Drug Discovery and Development Feng Wang, 2008-03-11 In this book, expert researchers provide a tool box for those who have a general interest in biomarker research and for those currently specializing in certain technologies but desiring an understanding of other available methodologies. Its chapters include validated, mature methods as well as new, incredibly promising protocols. This book is the perfect biomarker technical guideline and reference to stimulate more exciting biomarker research and technology development. |
| destain solution for coomassie: Evidence-based Research Methods for Chinese Medicine Siu-wai Leung, Hao Hu, 2016-11-24 This book is a practical guide to the evidence-based medicine (EBM) research methods that are applicable to Chinese medicine (CM). It includes 3 parts: clinical research, healthcare data research and laboratory research. In each chapter, it explains how to acquire, analyze and evaluate a specific kind of CM research result. Authors exemplify the most important methods with successful cases in transforming reliable data into new knowledge of CM. This book emphasizes the requirements for CM researchers to design and conduct CM studies by following the best available principles, standards and guidelines. |
| destain solution for coomassie: Laboratory Methods in Enzymology: Protein Part C , 2014-03-22 In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on Protein |
| destain solution for coomassie: Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology Keith Wilson, Andreas Hofmann, John M. Walker, Samuel Clokie, 2018-04-19 A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research. |
| destain solution for coomassie: Protein Analysis and Purification Ian M. Rosenberg, 2013-12-01 How one goes about analyzing proteins is a constantly evolving field that is no longer solely the domain of the protein biochemist. Investi gators from diverse disciplines find themselves with the unanticipated task of identifying and analyzing a protein and studying its physical properties and biochemical interactions. In most cases, the ultimate goal remains understanding the role(s) that the target protein is playing in cellular physiology. It was my intention that this manual would make the initial steps in the discovery process less time consuming and less intimidating. This book is not meant to be read from cover to cover. The expanded Table of Contents and the index should help locate what you are seeking. My aim was to provide practically oriented information that will assist the experimentalist in benchtop problem solving. The appendices are filled with diverse information gleaned from catalogs, handbooks, and manuals that are presented in a distilled fashion designed to save trips to the library and calls to technical service representatives. The user is encouraged to expand on the tables and charts to fit individual experimental situations. This second edition pays homage to the computer explosion and the various genome projects that have revolutionized how benchtop scientific research is performed. Bioinformatics and In silica science are here to stay. However, the second edition still includes recipes for preparing buffers and methods for lysing cells. |
| destain solution for coomassie: Oxygen Sensing , 2004-05-10 The ability of cells to sense and respond to changes in oxygenation underlies a multitude of developmental, physiological, and pathological processes. This volume provides a comprehensive compendium of experimental approaches to the study of oxygen sensing in 48 chapters that are written by leaders in their fields. |
| destain solution for coomassie: Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions Clarice M. Yentsch, Frances C. Mague, Paul K. Horan, 2013-03-13 This volume represents a collection of pioneering papers introducing immunochemical techniques to the aquatic sciences. It is the product of a workshop entitled Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions held at the University of Southern Maine, Portland, Oct. 5-7, 1986. Funding from many sources made this workshop possible, and is gratefully acknowledged. The reader will note great variability in both the quality and scope of the papers which we have divided 'into four sections: I. Background material on the immune system (borrowed from a recent NIH publication) II. Background material related to aquatic sciences III. Techniques IV. Applications Readers familiar with terminology are directed to start with the original contributions related to aquatic sciences, page 21. Some of the contributions are abstracts only. Others are preliminary, for which we are grateful to the authors for their willingness to share recent sometimes tentative findings with a broader community. Some offer novel and tempting insights, but as they exist, lack adequate standards and controls. Others are thorough and represent major scientific contributions. The promise rests in generating and working with reagents of great specificity enabling orders of magnitude improvements in the sensitivity of our measurements. Authors also address the problems and limitations. We thank the numerous authors and reviewers. In support of every successful endeavor is someone of unusual savvy. For us, this has been David A. Phinney. His leadership, wisdom and wit have been important to the introduction of flow cytometry/sorting and immunochemistry into aquatic research. |
| destain solution for coomassie: Proteomics C. David O'Connor, B. David Hames, 2007-11-01 Proteomics: Methods Express identifies the most powerful new technologies and presents them in a way that allows their robust implementation. The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house. Every chapter discusses the merits and limitations of various approaches then provides tried-and-tested protocols with hints and tips for success and troubleshooting for when things go wrong. |
| destain solution for coomassie: Checkpoint Controls and Cancer Axel H. Schönthal, 2008-02-03 Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors. Ext- nal stresses can be generated by the continuous assault of DNA-damaging agents, such as environmental mutagens, ultraviolet (UV) light, ionizing radiation, or the reactive oxygen species that can arise during normal cellular metabolism. In response to any of these assaults on the integrity of the genome, the activation of the network of checkpoint control pathways can lead to diverse cellular responses, such as cell cycle arrest, DNA repair, or elimination of the cell by cell death (apoptosis) if the damage cannot be repaired. Moreover, internal errors can occur during the highly orchestrated replication of the cellular genome and its distribution into daughter cells. Here, the temporal order of these cell cycle events must be strictly enforced—for example, to ensure that DNA replication is c- plete and occurs only once before cell division, or to monitor mitotic spindle assembly, and to prevent exit from mitosis until chromosome segregation has been completed. Thus, well functioning checkpoint mechanisms are central to the maintenance of genomic integrity and the basic viability of cells and, the- fore, are essential for proper development and survival. The importance of proper functioning of checkpoints becomes plainly obvious under conditions in which this control network malfunctions and fails. Depending on the severity and timing, failure of this machinery can lead to embryonic lethality, genetic diseases, and cancer. |
| destain solution for coomassie: Chemical Tools in Microbiology 1 , 2022-03-22 Clinical Microbiology, Volume 664 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Synthesis of chemical probes to study bacterial adenylating enzymes, Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux, Combining informatics with ABPP to identify serine hydrolyses in bacteria, A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS-CoV-2, Activity-based probes for bacterial histidine kinases, Metabolomic approaches to enzyme function and pathway discovery, Identification of bile salt hydrolase activity in gut microbiota, and much more. Other chapters cover Multiplex fluorescence screening and identification using multiplex TMT, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via SPR, Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry, Proteomics of short-chain fatty acid probes in Salmonella, Development and application of highly sensitive labeling reagents for amino acids, and a variety of other timely topics. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on Chemical Microbiology |
| destain solution for coomassie: Zone Electrophoresis Ivor Smith, 2013-10-22 Chromatographic & Electrophoretic Techniques, Fourth Edition, Volume II: Zone Electrophoresis presents the methods of zone electrophoresis. This book discusses all the techniques that can be applied routinely. Organized into 17 chapters, this volume begins with an overview of the fundamental principle of zone electrophoresis. This text then examines the apparatus in common use for paper electrophoresis and discusses paper electrophoresis at low voltages. Other chapters describe the apparatus that combines several advantages resulting from high voltage paper electrophoresis and thin-layer chromatography. This book discusses as well the advantages of cellulose acetate membrane filter paper as a supporting medium for zone electrophoresis. The final chapter deals with preparative acrylamide gel electrophoresis on a small scale, which may involve either continuous electrophoretic elution or extraction of the RNA from zones cut out of the gel. This book is a valuable resource for biochemists, clinical chemists, clinicians, technicians, biophysicists, pathologists, and medical researchers. |
| destain solution for coomassie: Mitochondria Dario Leister, Johannes M. Herrmann, 2007-06-12 Mitochondrial Genomics and Proteomics Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis. |
| destain solution for coomassie: Cell Biology Julio E Celis, 2014-05-10 Cell Biology: A Laboratory Handbook, Volume 3 is a handbook on cell biology and covers topics ranging from transfer of macromolecules and small molecules to cloning of embryos, transgenics, and gene targeting. Cell-free extracts, permeabilized cell systems, and expression systems are also discussed, along with proteins. Comprised of 58 chapters, this volume begins with a detailed account of microinjection of RNA, DNA, and proteins into somatic cells, followed by an analysis of computer-automated capillary microinjection of macromolecules into living cells. The reader is then introduced to syringe loading as a method for inserting macromolecules into cells in suspension; electroporation of cells; and the use of liposomes in drug targeting. Subsequent chapters focus on the cloning of rabbit embryos by nuclear transplantation; gene targeting by homologous recombination in embryonic stem cells; production and isolation of recombinant viruses; and gel electrophoresis. This book will be of interest to geneticists and molecular biologists. |
| destain solution for coomassie: Seed Analysis Hans-Ferdinand Linskens, John F. Jackson, 2013-11-11 Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of ex isting and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized jour nals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is dif ficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, com plete in itself with little need to consult other publications. Contribution authors have attempted to follow these guidelines in this New Series of volumes. |
| destain solution for coomassie: Food Analysis Laboratory Manual S. Suzanne Nielsen, 2010-03-20 This second edition laboratory manual was written to accompany Food Analysis, Fourth Edition, ISBN 978-1-4419-1477-4, by the same author. The 21 laboratory exercises in the manual cover 20 of the 32 chapters in the textbook. Many of the laboratory exercises have multiple sections to cover several methods of analysis for a particular food component of characteristic. Most of the laboratory exercises include the following: introduction, reading assignment, objective, principle of method, chemicals, reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and references. This laboratory manual is ideal for the laboratory portion of undergraduate courses in food analysis. |
| destain solution for coomassie: Monoclonal Antibodies Heddy Zola, 2013-11-20 This book describes, in detail, tested techniques for the produc-tion and use of monoclonal antibodies. It covers those aspects of interest to all scientists working with monoclonal antibodies and presents methods in a step-by-step format for easy refer-ence. The text serves as a laboratory manual; and discusses rationale behind each method, and th |
| destain solution for coomassie: Handbook of Food Analytical Chemistry, Volume 1 Ronald E. Wrolstad, Terry E. Acree, Eric A. Decker, Michael H. Penner, David S. Reid, Steven J. Schwartz, Charles F. Shoemaker, Denise M. Smith, Peter Sporns, 2005-09-02 Emphasizing effective, state-of-the art methodology and written by recognized experts in the field, the Handbook of Food Analytical Chemistry is an indispensable reference for food scientists and technologists to enable successful analysis. * Provides detailed reports on experimental procedures * Includes sections on background theory and troubleshooting * Emphasizes effective, state-of-the art methodology, written by recognized experts in the field * Includes detailed instructions with annotated advisory comments, key references with annotation, time considerations and anticipated results |
| destain solution for coomassie: Issues in Biochemistry and Geochemistry: 2011 Edition , 2012-01-09 Issues in Biochemistry and Geochemistry / 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Biochemistry and Geochemistry. The editors have built Issues in Biochemistry and Geochemistry: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Biochemistry and Geochemistry in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Biochemistry and Geochemistry / 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/. |
| destain solution for coomassie: Breast Cancer Research Protocols Susan A. Brooks, Adrian Harris, 2008-02-02 A collection of both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes. |
| destain solution for coomassie: Proteomic Methods in Neuropsychiatric Research Paul C. Guest, 2017-03-28 Due to continuous technical developments and new insights into the high complexity of neurological diseases, there is an increasing need for the application of proteomic technologies which can yield potential biomarker readouts for improved clinical management as well as for the development of new drugs by struggling pharmaceutical companies. This book describes the step-by-step use of proteomic methods such as two-dimensional gel electrophoresis, multiplex immunoassay, liquid chromatography mass spectrometry (LC-MS) and selective reaction monitoring MS, to increase our understanding of these diseases, with the ultimate aim of improving patient care. The volume will be of high interest to clinical scientists, physicians and pharmaceutical company scientists as it gives insights into the latest technologies enabling the revolution of personalized medicine. It is of direct interest to both technical and bench biomarker scientists as it gives step by step instructions on how to carry out each of the protocols. It is also of interest to researchers as each technique will be presented in the context of a specific neurological disorder, including Alzheimer's disease, multiple sclerosis, autism spectrum disorders, schizophrenia, major depressive disorder and bipolar disorder. Finally, it will also highlight the future research efforts in this field, which are endeavoring to convert proteomic platforms to the form of hand held devices which can be used in a point of care setting and return diagnostic results within the timeframe of a visit to the general practitioner. |
| destain solution for coomassie: Practical Protocols in Molecular Biology Yongming Li, Yuqi Zhao, 1996 |
| destain solution for coomassie: Molecular Diagnosis of Infectious Diseases Jochen Decker, Udo Reischl, 2008-02-01 Populations of the western world are now healthier and enjoying higher life expectancy than ever. They are beginning to benefit from an array of costly new therapies made possible through recent rapid advances in medical science and technology, and their demands on modern medicine are rising. Meanwhile, healthcare systems are struggling with their outdated legacy models of the m- th 20 century and are experiencing ever-increasing financial pressure from g- ernments and health insurance organizations. The equation is no longer in balance, and this predicament is forcing societies to explore new approaches to managing healthcare in the future. Since the first edition of Molecular Diagnosis of Infectious Diseases was published, we have witnessed the sequencing of the (almost) complete human genome and a shift in medical research from an emphasis on genetics to the advancement and useful application of proteomics. Bioinformatics has become the key tool for managing and analyzing the upsurge of data, and faster and more effective test methods and technologies have opened up new prospects for ind- try and academia. The tools of modern genomics and proteomics are now being utilized to specifically guide the discovery of drugs for the prevention, diagnosis, and treatment of human disease. They may also help us to find a way out of the current healthcare calamity. |
| destain solution for coomassie: Thiol Redox Transitions in Cell Signaling, Part B , 2010-08-19 This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. - Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling - Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines |
| destain solution for coomassie: Western Blotting for the Non-Expert Biji T. Kurien, 2021-07-24 This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes.. |
| destain solution for coomassie: Electrophoresis Fundamentals Budin Michov, 2022-04-04 The electrophoresis techniques are used in medicine, biochemistry, analytical chemistry, and biology to separate soluble and insoluble proteins, nucleic acids, chromosomes, viruses, as well as lysosomes, mitochondria, ribosomes and other cell organelles, red cells, tissue cells, and parasites. This book provides a view over the old electrophoresis techniques, as well as the recent developments in electrophoresis. Electrophoresis Fundamentals is based on the recent book Electrophoresis: Theory and Practice published in 2020 by De Gruyter. The previous book combines theory and technical applications with troubleshooting and problem solving. While Electrophoresis is intended for specialists, Electrophoresis Fundamentals is a book for laboratory technicians, students, biochemists, general practitioners, and more. |
| destain solution for coomassie: Metastasis Research Protocols Susan A. Brooks, Udo Schumacher, 2008-02-02 The process of metastasis formation is hugely complex, as described in the introductory chapter of this book, and this complexity has led us to compile two volumes of methods, from a vastly divergent background that attempts to encompass the whole spectrum of cancer biology. This first volume, Metastasis Research Protocols: Analysis of Cells and Tissues, concentrates on analysis and mapping of molecules produced by cells and tissues and analysis of the molecular biology underlying their expression, whereas the second volume, Metastasis Research Protocols: Cell Behavior In Vitro and In Vivo,focuses sharply on the determination of cell behavior in vitro and in vivo. We have deliberately included chapters describing well-established and familiar te- niques (for example, SDS-PAGE and Western blotting [Chapter 11], and immunocytochemistry [Chapter 2]) in addition to the newer and more speci- ized approaches and specific examples of their application, because—although the methodology is readily available in the published literature and established in many laboratories—we wished these volumes to “stand alone” and to make accessible here the standard techniques that underpin much metastasis research for both the newcomer to the field and the seasoned researcher. Undoubtedly, owing to the complexity of the metastatic cascade and the wealth of research techniques involved in scientific approaches to its unraveling, and despite our best efforts to make these volumes as comprehensive as seems feasible, this is a tall order, and there will inevitably be omissions. For these we apologize. |
| destain solution for coomassie: Electrophoresis Budin Michov, 2020-09-07 Electrophoresis is a straightforward but informative analytical method used in biochemistry, biology and medicine. This book combines a detailed discussion of theory and technical application with an elaborate section on troubleshooting and problem solving in electrophoresis. Therefore the book is an important guide for both students and scientists. |
Protocol for Staining Gels with Coomassie Blue G-250
3. Destain with 40% HPLC grade methanol/ 10% acetic acid, replacing the solution every 10-20 minutes until faint bands are observed. Kimwipes rolled up into balls can be added to speed up …
Staining of Gels with Coomassie Blue R-250 - Frederick …
5.3 Remove the stain solution, add the Fixative/Destain Solution and incubate with agitation in order to destain the gel. This solution can be replaced with fresh solution to promote
SAFETY DATA SHEET - The Wercs
Coomassie Brilliant Blue R-250 Destain Solution Revision date 15-Feb-2023 Precautionary Statements - Prevention Wash face, hands and any exposed skin thoroughly after handling
Coomassie Blue Staining - Bioscience
Coomassie Blue R250 is an anionic dye that stoichiometrically binds to proteins, and is therefore preferable to silver staining methods for estimation of relative abundance of proteins useful for …
QC Colloidal Coomassie Stain - Bio-Rad
Bio-Rad’s QC colloidal Coomassie stain is a ready-to-use single-bottle protein stain that does not require the mixing of any components or addition of any alcohols. It is a special formulation of …
Denaturing Reduced Protein Gels, Coomassie Staining, and …
Stain the membrane with Coomassie blue stain for 7 minutes (on orbital shaker 75 rpm) in a rectangular petri dish. 1. Pour off excess Coomassie blue stain into the container labeled ‘Used …
protective equipment. Gel-Clear Destain Solution Gel-Clear …
After running, transfer the gels to a 1.4 quart microwave-safe, round, plastic container. Add 75 ml (or enough to submerge gels) of fresh staining solution (0.1% Coomassie Blue R250 in 40% …
Coomassie Blue Staining Method - Sinica
1. Fix gel in Fixing solution for 1 hr to overnight with gentle agitation. Change solution once at first 1 hr. 2. Stain gel in Staining solution for 20 min with gentle agitation. 3. Destain gel in …
A. Reagents - Children's Cancer Hospital Egypt
Coomassie Stain Protocol A. Reagents: – Coomassie Brilliant Blue (CBBR-250) stain: 0.1% CBBR-250, 10% acetic acid, 50% methanol. 1. Stir for 4 hours to overnight. 2. Filter through …
SOP: SP013.1 Coomassie Staining Protocol Materials and …
_____ Pour off first fix and add approximately 5 to 10 gel volumes of Coomassie destain solution (or 50% Methanol for LC-MS samples). Let rock until background is clear and only protein …
University of Melbourne iGEM 2014 Lab Procedure
How to perform a Coomassie stain on a SDS-PAGE membrane. Use this procedure after having performed SDS-PAGE. Place the SDS-PAGE membrane to be stained in a container with a lid. …
Rapid Staining Solution for Coomassie Brilliant Blue
Blue Fast Staining Solution (Coomassie Blue Fast Staining Solution) is based on Coomassie Blue G250 as the dye, and can be used for non-contaminating, rapid and highly sensitive staining of …
COOMASSIE STAINING PROTEIN GELS - langdalelab.com
COOMASSIE STAINING PROTEIN GELS 1. Stain for 5-30’ in stain solution, shaking at room temperature. Actual time depends on thickness of the gel. Minigels are OK with 5’. 2. Destain …
Coomassie Blue Staining Methods in the Multi 6-
staining solution: 0.03 % (w/v) Coomassie R-350 (2 tablets) in 2.5 L 12.5% acetic acid. Pour 600 mL into the staining tray standard size; staining tray large size: 1.2 L; multi6: 6 L. Stir while …
Coomassie® Brilliant Blue R 250 Powder - SERVA
For a first rapid destain, incubate gel for ca. 0.5 - 5 min. (dependent on gel thickness) in destainer I. Destain gel further as long as needed in destainer II (visual inspection).
Coomassie Stain of Protein Gel - fredhutch.org
Coomassie Stain of Protein Gel Hahn Lab, 2001 1. After electrophoresis of protein gel, transfer gel to round staining tray. Add ~200 ml protein gel stain. 2. Microwave for ~45 sec until the solution …
Superfast-Blue Coomasie Stain
staining solution specially formulated for nonhazardous sensitive detection of proteins. It is based on the colloidal properties of Coomassie Blue dyes created in aqueous solutions containing …
SAFETY DATA SHEET - bio-rad-sds.thewercs.com
Coomassie Brilliant Blue R-250 Destain Solution Revision date 15-Feb-2023 heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. Use grounding and bonding …
Polyacrylamide Gel Staining Protocols - ResearchGate
• Coomassie Working solution: 500 mL Methanol+ 30 mL Coomassie concentrated stain solution + 400 mL milli-Q water + 100 mL acetic acid. Mix and filter using a 0.22 micron presterilized...
DESTAIN Definition & Meaning - Merriam-Webster
The meaning of DESTAIN is to selectively remove stain from (a specimen for microscopic study).
DESTAIN Definition & Meaning | Dictionary.com
to remove stain (from a specimen) to enhance visibility and contrast of parts. Examples have not been reviewed. I feel badly that so many folks view this article with either destain or negativity. …
destain, v. meanings, etymology and more | Oxford English …
Factsheet What does the verb destain mean? There is one meaning in OED's entry for the verb destain. See ‘Meaning & use’ for definition, usage, and quotation evidence.
DESTAIN definition and meaning | Collins English Dictionary
2 meanings: chemistry to remove a stain from → an archaic spelling of distain.... Click for more definitions.
Destain - Definition, Meaning & Synonyms | Vocabulary.com
5 days ago · /diˈsteɪn/ IPA guide Other forms: destains; destained Definitions of destain verb remove stain from (a laboratory specimen) to enhance contrast see more
Destain - definition of destain by The Free Dictionary
Define destain. destain synonyms, destain pronunciation, destain translation, English dictionary definition of destain. tr.v. de·stained , de·stain·ing , de·stains To remove stain from to aid in …
destain - Wiktionary, the free dictionary
May 16, 2024 · destain (third-person singular simple present destains, present participle destaining, simple past and past participle destained) (transitive) To remove a chemical stain …
destain - WordReference.com Dictionary of English
de•stain (dē stān′), v.t. [Histol.] to remove stain (from a specimen) to enhance visibility and contrast of parts. No titles with the word (s) "destain". Visit the English Only Forum. Help …
What does Destain mean? - Definitions.net
Destain refers to the process or action of removing stains or dyes from a material or surface. This term is often used in biochemistry and molecular biology to refer to the removal of excess stain …
DESTAIN - Definition & Meaning - Reverso English Dictionary
Destain definition: remove a chemical stain from a surface. Check meanings, examples, usage tips, pronunciation, domains, related words.
