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| dynamic light scattering data analysis: Introduction to Dynamic Light Scattering by Macromolecules Kenneth S Schmitz, 2012-12-02 An Introduction to Dynamic Light Scattering by Macromolecules provides an introduction to the basic concepts of dynamic light scattering (DLS), with an emphasis on the interpretation of DLS data. It presents the appropriate equations used to interpret DLS data. The material is presented in order of increasing complexity of the systems under examination, ranging from dilute solutions of noninteracting particles to concentrated multicomponent solutions of strongly interacting particles and gels. Problems are presented at the end of each chapter to emphasize these concepts. Since a major emphasis of this textbook is the interpretation of DLS data obtained by polarized light scattering studies on macromolecular solutions, the results of complementary experimental techniques are also presented in order to gain insight into the dynamics of these systems. This textbook is intended for (1) advanced undergraduate students and graduate students in the chemical, physical, and biological sciences; (2) scientists who might wish to apply DLS methods to systems of interest to them but who have no formal training in the field of DLS; and (3) those who are simply curious as to the type of information that might be obtained from DLS techniques. |
| dynamic light scattering data analysis: Dynamic Light Scattering Wyn Brown, 1993-01-28 Dynamic light scattering is an experimental technique now commonly found in laboratories concerned with fundamental studies of macromolecular systems--Preface. |
| dynamic light scattering data analysis: Measurement Techniques and Practices of Colloid and Interface Phenomena Masahiko Abe, 2019-03-30 This book is a manual of measurement of colloids and interfaces designed especially for new researchers who have just begun research on these topics. The book is written by active researchers in the field of colloids and interfacial chemistry, based on the practical experience of the authors. In each chapter, the key points of measurement, how to analyze data correctly, points to be careful about, and merits of a particular method are concisely explained from the point of view of the readers. Not only in industries such as cosmetics and pharmaceuticals but also in academic studies of nanotechnology, correct understanding of colloid and interface phenomena is vital because the properties of these items, however small, are affected by the nature of interfaces. This book will be particularly useful for researchers who are not yet fully confident of the measurement techniques that are clearly explained here. |
| dynamic light scattering data analysis: Soft-Matter Characterization Redouane Borsali, Robert Pecora, 2008-07-28 This 2-volume set includes extensive discussions of scattering techniques (light, neutron and X-ray) and related fluctuation and grating techniques that are at the forefront of this field. Most of the scattering techniques are Fourier space techniques. Recent advances have seen the development of powerful direct imaging methods such as atomic force microscopy and scanning probe microscopy. In addition, techniques that can be used to manipulate soft matter on the nanometer scale are also in rapid development. These include the scanning probe microscopy technique mentioned above as well as optical and magnetic tweezers. |
| dynamic light scattering data analysis: Light Scattering Reviews 4 Alexander A. Kokhanovsky, 2009-07-25 This fourth volume of Light Scattering Reviews is composed of three parts. The ?rstpartisconcernedwiththeoreticalandexperimentalstudiesofsinglelightsc- tering by small nonspherical particles. Light scattering by small particles such as, for instance, droplets in the terrestrial clouds is a well understood area of physical optics. On the other hand, exact theoretical calculations of light scattering p- terns for most of nonspherical and irregularly shaped particles can be performed only for the restricted values of the size parameter, which is proportional to the ratio of the characteristic size of the particle to the wavelength?. For the large nonspherical particles, approximations are used (e. g. , ray optics). The exact th- retical techniques such as the T-matrix method cannot be used for extremely large particles, such as those in ice clouds, because then the size parameter in the v- iblex=2?a/???,wherea is the characteristic size (radius for spheres), and the associated numerical codes become unstable and produce wrong answers. Yet another problem is due to the fact that particles in many turbid media (e. g. , dust clouds) cannot be characterized by a single shape. Often, refractive indices also vary. Because of problems with theoretical calculations, experimental (i. e. , la- ratory) investigations are important for the characterization and understanding of the optical properties of such types of particles. The ?rst paper in this volume, written by B. Gustafson, is aimed at the descr- tionofscaledanalogueexperimentsinelectromagneticscattering. |
| dynamic light scattering data analysis: Dynamic and Electrophoretic Light Scattering Cornelia Bellmann, Anja Caspari, Christian Moitzi, Thomas Luxbacher, Michael Schäffler, Michael Stintz, 2019 |
| dynamic light scattering data analysis: Dynamic Light Scattering R. Pecora, 2013-11-11 In the twenty years since their inception, modern dynamic light-scattering techniques have become increasingly sophisticated, and their applications have grown exceedingly diverse. Applications of the techniques to problems in physics, chemistry, biology, medicine, and fluid mechanics have prolifer ated. It is probably no longer possible for one or two authors to write a monograph to cover in depth the advances in scattering techniques and the main areas in which they have made a major impact. This volume, which we expect to be the first of aseries, presents reviews of selected specialized areas by renowned experts. It makes no attempt to be comprehensive; it emphasizes a body of related applications to polymeric, biological, and colloidal systems, and to critical phenomena. The well-known monographs on dynamic light scattering by Berne and Pecora and by Chu were published almost ten years ago. They provided comprehensive treatments of the general principles of dynamic light scat tering and gave introductions to a wide variety of applications, but natu rally they could not treat the new applications and advances in older ones that have arisen in the last decade. The new applications include studies of interacting particles in solution (Chapter 4); scaling approaches to the dynamics of polymers, including polymers in semidilute solution (Chapter 5); the use of both Fabry-Perot interferometry and photon correlation spectroscopy to study bulk polymers (Chapter 6); studies of micelIes and microemulsions (Chapter 8); studies of polymer gels (Chapter 9). |
| dynamic light scattering data analysis: Dynamic Light Scattering Bruce J. Berne, Robert Pecora, 2013-07-24 Lasers play an increasingly important role in a variety of detection techniques, making inelastic light scattering a tool of growing value in the investigation of dynamic and structural problems in chemistry, biology, and physics. Until the initial publication of this work, however, no monograph treated the principles behind current developments in the field.This volume presents a comprehensive introduction to the principles underlying laser light scattering, focusing on the time dependence of fluctuations in fluid systems; it also serves as an introduction to the theory of time correlation functions, with chapters on projection operator techniques in statistical mechanics. The first half comprises most of the material necessary for an elementary understanding of the applications to the study of macromolecules, or comparable sized particles in fluids, and to the motility of microorganisms. The study of collective (or many particle) effects constitutes the second half, including more sophisticated treatments of macromolecules in solution and most of the applications of light scattering to the study of fluids containing small molecules.With its wide-ranging discussions of the many applications of light scattering, this text will be of interest to research chemists, physicists, biologists, medical and fluid mechanics researchers, engineers, and graduate students in these areas. |
| dynamic light scattering data analysis: Radiation in Bioanalysis , 2019 This book describes the state of the art across the broad range of spectroscopic techniques used in the study of biological systems. It reviews some of the latest advances achieved in the application of these techniques in the analysis and characterization of small and large biological compounds, covering topics such as VUV/UV and UV-visible spectroscopies, fluorescence spectroscopy, IR and Raman techniques, dynamic light scattering (DLS), circular dichroism (CD/SR-CD), pulsed electron paramagnetic resonance techniques, Mössbauer spectroscopy, nuclear magnetic resonance, X-ray methods and electron and ion impact spectroscopies. The second part of the book focuses on modelling methods and illustrates how these tools have been used and integrated with other experimental and theoretical techniques including also electron transfer processes and fast kinetics methods. The book will benefit students, researchers and professionals working with these techniques to understand the fundamental mechanisms of biological systems. |
| dynamic light scattering data analysis: Food Polymers, Gels and Colloids E. Dickinson, 1991-01-01 Manufactured foodstuffs typically exist in the form of complex, multi-phase, multi-component, colloidal systems. One way to try to make sense of their chemical and structural complexity is to study simple model systems in which the nature and properties of the polymer molecules and dispersed particles are relatively well known. This volume consists of a collection of papers delivered at a conference on food colloids, the main theme of which was the role of food macromolecules in determining the stability, structure, texture and rheology of food colloids, with particular reference to gelling behaviour and interactions between macromolecules and interfaces. A feature of the collection is the wide range of physico-chemical techniques now being used to address problems in this field. |
| dynamic light scattering data analysis: Light Scattering from Polymer Solutions and Nanoparticle Dispersions Wolfgang Schärtl, 2007-08-13 Light scattering is a very powerful method for characterizing the structure of polymers and nanoparticles in solution. As part of the Springer Laboratory series, this book provides a simple-to-read and illustrative textbook probing the seemingly very complicated topic of light scattering from polymers and nanoparticles in dilute solution, and goes further to cover some of the latest technical developments in experimental light scattering. |
| dynamic light scattering data analysis: Particle Characterization: Light Scattering Methods Renliang Xu, 2006-04-11 Particle characterization is an important component in product research and development, manufacture, and quality control of particulate materials and an important tool in the frontier of sciences, such as in biotechnology and nanotechnology. This book systematically describes one major branch of modern particle characterization technology - the light scattering methods. This is the first monograph in particle science and technology covering the principles, instrumentation, data interpretation, applications, and latest experimental development in laser diffraction, optical particle counting, photon correlation spectroscopy, and electrophoretic light scattering. In addition, a summary of all major particle sizing and other characterization methods, basic statistics and sample preparation techniques used in particle characterization, as well as almost 500 latest references are provided. The book is a must for industrial users of light scattering techniques characterizing a variety of particulate systems and for undergraduate or graduate students who want to learn how to use light scattering to study particular materials, in chemical engineering, material sciences, physical chemistry and other related fields. |
| dynamic light scattering data analysis: RNA Spectroscopy Véronique Arluison, Frank Wien, 2020-02-01 This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins. The chapters in this book cover topics such as single-molecule spectroscopy of multiple RNA species; surface plasmon resonance, MS or microcalorimetry for investigating molecular interactions with RNA; FTIR, SAXS, SANS and SRCD spectroscopies to analyze RNA structure; use of fluorescent nucleotides to map RNA-binding sites on proteins surfaces or CryoEM; and much more. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Spectroscopy: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field. |
| dynamic light scattering data analysis: Bacterial Chromatin Remus T. Dame, |
| dynamic light scattering data analysis: Light Scattering by Ice Crystals Kuo-Nan Liou, Ping Yang, 2016-10-06 This volume outlines the fundamentals and applications of light scattering, absorption and polarization processes involving ice crystals. |
| dynamic light scattering data analysis: Single-Chain Polymer Nanoparticles José A. Pomposo, 2017-08-18 This first book on this important and emerging topic presents an overview of the very latest results obtained in single-chain polymer nanoparticles obtained by folding synthetic single polymer chains, painting a complete picture from synthesis via characterization to everyday applications. The initial chapters describe the synthetics methods as well as the molecular simulation of these nanoparticles, while subsequent chapters discuss the analytical techniques that are applied to characterize them, including size and structural characterization as well as scattering techniques. The final chapters are then devoted to the practical applications in nanomedicine, sensing, catalysis and several other uses, concluding with a look at the future for such nanoparticles. Essential reading for polymer and materials scientists, materials engineers, biochemists as well as environmental chemists. |
| dynamic light scattering data analysis: Soft Nanoparticles for Biomedical Applications José Callejas-Fernández, Joan Estelrich, Manuel Quesada-Pérez, Jacqueline Forcada, 2014-06-18 Nanoparticles are attractive for many biomedical applications such as imaging, therapeutics and diagnostics. This new book looks at different soft nanoparticles and their current and potential uses in medicine and health including magnetoliposomes, micro/nanogels, polymeric micelles, DNA particles, dendrimers and bicelles. Each chapter provides a description of the synthesis of the particles and focus on the techniques used to characterize the size, shape, surface charge, internal structure, and surface microstructure of the nanoparticles together with modeling and simulation methods. By giving a strong physical-chemical approach to the topic, readers will gain a good background into the subject and an overview of recent developments. The multidisciplinary point of view makes the book suitable for postgraduate students and researchers in physics, chemistry, and biology interested in soft matter and its uses. |
| dynamic light scattering data analysis: Handbook of Materials Characterization Surender Kumar Sharma, 2018-09-18 This book focuses on the widely used experimental techniques available for the structural, morphological, and spectroscopic characterization of materials. Recent developments in a wide range of experimental techniques and their application to the quantification of materials properties are an essential side of this book. Moreover, it provides concise but thorough coverage of the practical and theoretical aspects of the analytical techniques used to characterize a wide variety of functional nanomaterials. The book provides an overview of widely used characterization techniques for a broad audience: from beginners and graduate students, to advanced specialists in both academia and industry. |
| dynamic light scattering data analysis: Laser Light Scattering Benjamin Chu, 2012-12-02 Laser Light Scattering: Basic Principles and Practice, Second Edition deals with the technical aspects of laser light scattering, including the basic principles and practice. Topics covered include light scattering theory, optical mixing spectrometry, photon correlation spectroscopy, and interferometry. Experimental methods and methods of data analysis are also described. This book is comprised of eight chapters and begins with a discussion on the interrelationship between laser light scattering and other types of scattering techniques that use X-rays and neutrons, with particular reference to momentum and energy transfers as well as time-averaged and time-dependent scattered intensity. The spectrum of scattered light and a single-particle approach to time-averaged scattered intensity are considered. The following chapters focus on photoelectric detection of the scattered electric field; optical mixing spectrometers; basic equations for photon correlation spectroscopy; and the principles of Fabry-Perot interferometry. The pertinent features of the experimental aspects of laser light scattering are also outlined, together with the Laplace inversion problem. The final chapter examines polymer molecular-weight distributions in relation to particle sizing. This monograph will be of interest to physicists. |
| dynamic light scattering data analysis: High Resolution Imaging in Microscopy and Ophthalmology Josef F. Bille, 2019-08-13 This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend. |
| dynamic light scattering data analysis: Dynamics of Proteins and Nucleic Acids , 2013-08-14 Published continuously since 1944, Advances in Protein Chemistry and Structural Biology has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each volume brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics. - Covers reviews of methodology and research in all aspects of protein chemistry - Brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics |
| dynamic light scattering data analysis: Evaluation Technologies for Food Quality Jian Zhong, Xichang Wang, 2019-04-16 Evaluation Technologies for Food Quality summarizes food quality evaluation technologies, which include sensory evaluation techniques and chemical and physical analysis. In particular, the book introduces many novel micro and nano evaluation techniques, such as atomic force microscopy, scanning electron microscopy, and other nanomaterial-based methods. All topics cover basic principles, procedures, advantages, limitations, recent technology development, and application progress in different types of foods. This book is a valuable resource for scientists in the field of food science, engineering, and professionals in the food industry, as well as for undergraduate and postgraduate students studying food quality evaluation technology. - Explains basic principles, procedures, advantages, limitations, and current applications of recent food quality technologies - Provides guidance on the understanding and application of food quality evaluation technology in the field of food research and food industry - Introduces many novel micro/nano evaluation techniques, such as atomic force and scanning electron microscopies and other nanomaterial-based methods |
| dynamic light scattering data analysis: Inorganic Frameworks as Smart Nanomedicines Alexandru Mihai Grumezescu, 2018-04-09 Inorganic Frameworks as Smart Nanocarriers for Drug Delivery brings together recent research in the area of inorganic frameworks for drug delivery. Different types of nanocarriers are presented and discussed in detail, providing an up-to-date overview on inorganic nanoparticles with pharmaceutical applications. Written by a diverse range of international academics, this book is a valuable reference resource for researchers in biomaterials, the pharmaceutical industry, and those who want to learn more about the current applications of inorganic smart nanocarriers. - Includes assembly methods for a variety of smart nanocarrier systems, also showing how they are applied - Highlights how metal-oxide nanoparticles are effectively used in drug delivery - Assesses the pros and cons of different metallic nanomaterials as drug carriers |
| dynamic light scattering data analysis: Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids Surendra Nimesh, Ramesh Chandra, Nidhi Gupta, 2017-04-04 Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids addresses several issues related to safe and effective delivery of nucleic acids (NAs) using nanoparticles. A further emphasis would be laid on the mechanism of delivery of NAs, the barriers encountered and the strategies adapted to combat them. An exhaustive account of the advantages as well shortcomings of all the delivery vectors being employed in delivery of various NAs will be provided. On final note the regulatory aspects of nanoparticles mediated NA would be discussed, with focus on their clinical relevance. The design and development of nucleic acid-based therapeutics for the treatment of diseases arising from genetic abnormalities has made significant progress over the past few years. NAs have been widely explored for the treatment of cancer and infectious diseases or to block cell proliferation and thereby caused diseases. Advances in synthetic oligonucleotide chemistry resulted in synthesis of NAs that are relatively stable in in vivo environments. However, cellular targeting and intracellular delivery of NAs still remains a challenge. Further development of NA-based therapeutics depends on the progress of safe and effective carriers for systemic administration. Nanomedicine has facilitated availability of vectors with diminished cytotoxicity and enhanced efficacy which are rapidly emerging as systems of choice. These vectors protect NAs from enzymatic degradation by forming condensed complexes along with targeted tissue and cellular delivery. During the past few years, a myriad reports have appeared reporting delivery of NAs mediated by nanoparticles. This book will provide an overview of nanoparticles being employed in the in vitro and in vivo delivery of therapeutically relevant NAs like DNA, siRNA, LNA, PNA, etc. - Provides a complete overview of the applicatiosn of nanomedicine in the delivery of nucleic acids, from characterization of nanoparticles, to in vitro and in vivo studies - Discusses delivery issues of less well explored nucleic acids, like PNAs, Ribozymes, DNAzymes, etc. - Summarizes the current state of research in nucleic acid delivery and underscores the future of nanomedicine in this field |
| dynamic light scattering data analysis: Targeting Chronic Inflammatory Lung Diseases Using Advanced Drug Delivery Systems Kamal Dua, Philip M. Hansbro, Ridhima Wadhwa, Mehra Haghi, Lisa G. Pont, Kylie A. Williams, 2020-08-04 Targeting Chronic Inflammatory Lung Diseases Using Advanced Drug Delivery Systems explores the development of novel therapeutics and diagnostics to improve pulmonary disease management, looking down to the nanoscale level for an efficient system of targeting and managing respiratory disease. The book examines numerous nanoparticle-based drug systems such as nanocrystals, dendrimers, polymeric micelles, protein-based, carbon nanotube, and liposomes that can offer advantages over traditional drug delivery systems. Starting with a brief introduction on different types of nanoparticles in respiratory disease conditions, the book then focuses on current trends in disease pathology that use different in vitro and in vivo models. The comprehensive resource is designed for those new to the field and to specialized scientists and researchers involved in pulmonary research and drug development. - Explores recent perspectives and challenges regarding the management and diagnosis of chronic respiratory diseases - Provides insights into how advanced drug delivery systems can be effectively formulated and delivered for the management of various pulmonary diseases - Includes the most recent information on diagnostic methods and treatment strategies using controlled drug delivery systems (including nanotechnology) |
| dynamic light scattering data analysis: Sequences of Proteins of Immunological Interest , 1991 Tabulation and analysis of amino acid and nucleic acid sequences of precursors, v-regions, c-regions, j-chain, T-cell receptors for antigen, T-cell surface antigens, l-microglobulins, major histocompatibility antigens, thy-1, complement, c-reactive protein, thymopoietin, integrins, post-gamma globulin, -macroglobulins, and other related proteins. |
| dynamic light scattering data analysis: Dairy Technology P. Walstra, 1999-04-23 Describes the efficient transformation of milk into a variety of products, focusing on the changes in raw material, and intermediate and final products, as well as the interactions between products and processing equipment. The book details the procedures for ensuring processing efficiency and product quality. |
| dynamic light scattering data analysis: Biophysical Characterization of Proteins in Developing Biopharmaceuticals Damian J. Houde, Steven A. Berkowitz, 2019-11-13 Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today's industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals. In addition, best practices of applying statistical analysis to biophysical characterization data is included, along with practical issues associated with the concept of a biopharmaceutical's developability and the technical decision-making process needed when dealing with biophysical characterization data. - Presents basic protein characterization methods and tools applicable to (bio)pharmaceutical research and development - Highlights the capabilities and limitations of each technique - Discusses the underlining science of each tool - Empowers industrial biophysical chemists by providing a roadmap for applying biophysical tools - Outlines the needs for new characterization and analytical tools in the biopharmaceutical industry |
| dynamic light scattering data analysis: Microrheology with Optical Tweezers Manlio Tassieri, 2016-10-14 Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to μs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)! Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies. The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the nature of light, followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book. |
| dynamic light scattering data analysis: Measurement of Suspended Particles by Quasi-elastic Light Scattering Barton E. Dahneke, 1983 |
| dynamic light scattering data analysis: Dynamic Light Scattering Imaging Anton Sdobnov, Evgenii Zherebtsov, Alexander Bykov, Igor Meglinski, 2024-10-18 This book examines Dynamic Light Scattering (DLS) and its derivatives Laser Doppler Flowmetry (LDF), Diffusing Wave Spectroscopy (DWS), Laser Speckle Contrast Imaging (LSCI), and Doppler Optical Coherence Tomography (OCT) for characterizing particle motion in turbid mediums like suspensions and solutions. It focuses on non-invasive blood flow imaging in biological tissues, detailing technological advancements, practical applications, and inherent challenges. Essential for professionals in biomedical optics and medical fields, as well as physics and engineering students, the book highlights its use in brain, skin, and micro-circulation studies, providing key insights and practical guidance. Key Features: • Presents a deep dive into DLS and its derivative techniques. • Emphasizes practical applications, including brain blood flow monitoring, skin perfusion measurements, and micro-circulation characterization. • Delivers insights into the challenges and limitations associated with DLS-based blood flow imaging. |
| dynamic light scattering data analysis: Bio-Nanoparticles Om V. Singh, 2015-06-22 Nanoparticles are the building blocks for nanotechnology; they are better built, long lasting, cleaner, safer, and smarter products for use across industries, including communications, medicine, transportation, agriculture and other industries. Controlled size, shape, composition, crystallinity, and structure-dependent properties govern the unique properties of nanotechnology. Bio-Nanoparticles: Biosynthesis and Sustainable Biotechnological Implications explores both the basics of and advancements in nanoparticle biosynthesis. The text introduces the reader to a variety of microorganisms able to synthesize nanoparticles, provides an overview of the methodologies applied to biosynthesize nanoparticles for medical and commercial use, and gives an overview of regulations governing their use. Authored by leaders in the field, Bio-Nanoparticles: Biosynthesis and Sustainable Biotechnological Implications bridges the gap between biology and technology, and is an invaluable resource for students and researchers alike. |
| dynamic light scattering data analysis: Data Evaluation in Light Scattering of Polymers Martin Helmstedt, Klaus Gast, 2000 This book is based on lectures and posters presented at the workshop Data Evaluation in Light Scattering of Polymersheld in Bad Schandau, Germany. The articles cover a broad range of applications including basic research studies on complex polymeric systems as well as routine measurements and provide a survey of novel data evaluation schemes mostly developed during the last decade. Additionally, this issue contains contributions dealing with novel and / or highly specialized light scattering experiments and with the combination of light scattering instruments with other experimental techniques. |
| dynamic light scattering data analysis: Comprehensive Biophysics , 2012-04-12 Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource |
| dynamic light scattering data analysis: Encapsulation and Controlled Release D R Karsa, R A Stephenson, 1993-01-01 Encapsulation and controlled release combines basic information on the subject with details of the latest research, making it suitable for both newcomers to the field and those with experience of encapsulation technology. It will also be of great interest to those working on water-soluble or dispersible polymers, as well as application chemists and biochemists in diverse areas. |
| dynamic light scattering data analysis: Molecular Characterization of Polymers Muhammad Imran Malik, Jimmy Mays, Muhammad Raza Shah, 2021-03-09 Molecular Characterization of Polymers presents a range of advanced and cutting-edge methods for the characterization of polymers at the molecular level, guiding the reader through theory, fundamentals, instrumentation, and applications, and supporting the end goal of efficient material selection and improved material performance. Each chapter focuses on a specific technique or family of techniques, including the different areas of chromatography, field flow fractionation, long chain branching, static and dynamic light scattering, mass spectrometry, NMR, X-Ray and neutron scattering, polymer dilute solution viscometry, microscopy, and vibrational spectroscopy. In each case, in-depth coverage explains how to successfully implement and utilize the technique. This practical resource is highly valuable to researchers and advanced students in polymer science, materials science, and engineering, and to those from other disciplines and industries who are unfamiliar with polymer characterization techniques. - Introduces a range of advanced characterization methods, covering aspects such as molecular weight, polydispersity, branching, composition, and tacticity - Enables the reader to understand and to compare the available technique, and implement the selected technique(s), with a view to improving properties of the polymeric material - Establishes a strong link between basic principles, characterization techniques, and real-life applications |
| dynamic light scattering data analysis: Nanotechnology for Oral Drug Delivery João Pedro Martins, Hélder A. Santos, 2020-07-27 Nanotechnology for Oral Drug Delivery: From Concept to Applications discusses the current challenges of oral drug delivery, broadly revising the different physicochemical barriers faced by nanotechnolgy-based oral drug delivery systems, and highlighting the challenges of improving intestinal permeability and drug absorption. Oral delivery is the most widely used form of drug administration due to ease of ingestion, cost effectiveness, and versatility, by allowing for the accommodation of different types of drugs, having the highest patient compliance. In this book, a comprehensive overview of the most promising and up-to-date engineered and surface functionalized drug carrier systems, as well as opportunities for the development of novel and robust delivery platforms for oral drug administration are discussed. The relevance of controlling the physicochemical properties of the developed particle formulations, from size and shape to drug release profile are broadly reviewed. Advances in both in vitro and in vivo scenarios are discussed, focusing on the possibilities to study the biological-material interface. The industrial perspective on the production of nanotechnology-based oral drug delivery systems is also covered. Nanotechnology for Oral Drug Delivery: From Concept to Applications is essential reading for researchers, professors, advanced students and industry professionals working in the development, manufacturing and/or commercialization of nanotechnology-based systems for oral drug delivery, targeted drug delivery, controlled drug release, materials science and biomaterials, in vitro and in vivo testing of potential oral drug delivery technologies. |
| dynamic light scattering data analysis: Gene therapy Surendra Nimesh, 2013-10-31 Gene therapy is emerging as a new class of therapeutics for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability of DNA in the physiological milieu limits its therapeutic potential, hence a vector which can protect and efficiently transport DNA to the target cells must be developed. Nanotechnology-based non-viral vectors have been proposed as potential candidates. Various polymeric nanoparticles have been shown to be suitable, with high cellular uptake efficiencies and reduced cytotoxicity. These delivery vectors form condensed complexes with DNA which result in shielding against enzymatic degradation and enhanced cellular targeting. Advantages including easy manipulatibility, high stability, low cost and high payload, mean that nanoparticles from various polymers have been exploited. Gene therapy gives a systematic account of the many aspects of nanotechnology mediated gene therapy, from the preparation of nanoparticles to physicochemical characterization, and follows with applications in in vitro and in vivo models. This book emphasizes the various aspects of nanotechnology-based gene therapy, with initial chapters detailing the tools and techniques available for preparation and in vitro and in vivo characterization of nanoparticles. Later chapters provide exhaustive details on polymeric systems employed for gene therapy. Provides an overview of nanotechnology applications in gene therapy, from preparation of nanoparticles to in vitro and in vivo studies Details the tools and techniques available for preparation, characterization and in vitro and in vivo study of nanoparticles Details the limitations of nanoparticle-mediated gene therapy and proposes ways in which they may be overcome |
| dynamic light scattering data analysis: Optical Properties of Advanced Materials Yoshinobu Aoyagi, Kotaro Kajikawa, 2013-05-14 In the last decade, optically functionalized materials have developed rapidly, from bulk matters to structured forms. Now we have a rich variety of attractive advanced materials. They are applied to optical and electrical devices that support the information communication technology in the mid 21-th century. Accordingly, it is quite important to have a broad knowledge of the optical properties of advanced materials for students, scientists and engineers working in optics and related fields. This book is designed to teach fundamental optical properties of such advanced materials effectively. These materials have their own peculiarities which are very interesting in modern optical physics and also for applications because the concepts of optical properties are quite different from those in conventional optical materials. Hence each chapter starts to review the basic concepts of the materials briefly and proceeds to the practical use. The important topics covered in this book include: quantum structures of semiconductors, spintronics, photonic crystals, surface plasmons in metallic nanostructures, photonic metamaterials, liquid crystal materials, organic LED materials and magnet-optics. |
| dynamic light scattering data analysis: The Mie Theory Wolfram Hergert, Thomas Wriedt, 2012-06-30 This book presents in a concise way the Mie theory and its current applications. It begins with an overview of current theories, computational methods, experimental techniques, and applications of optics of small particles. There is also some biographic information on Gustav Mie, who published his famous paper on the colour of Gold colloids in 1908. The Mie solution for the light scattering of small spherical particles set the basis for more advanced scattering theories and today there are many methods to calculate light scattering and absorption for practically any shape and composition of particles. The optics of small particles is of interest in industrial, atmospheric, astronomic and other research. The book covers the latest developments in divers fields in scattering theory such as plasmon resonance, multiple scattering and optical force. |
Interpreting and Understanding Dynamic Light Scattering …
What is Dynamic Light Scattering? Dynamic light scattering refers to measurement and interpretation of light scattering data on a microsecond time scale. Dynamic light scattering …
DLS Data Interpretation - Entegris
The Entegris Nicomp® dynamic light scattering (DLS) system is an easy to use particle size and zeta potential analyzer. This technical note shows typical results from the Nicomp and explains …
SCATTERING PARTICLE SIZE ANALYSIS BY DYNAMIC LIGHT
This method is based on ISO standard 22412:2017, Particle size analysis—Dynamic light scattering (DLS). Portions of the chapter text that are national USP text, and are not part of the …
Dynamic Light Scattering Training - University of California, …
What is Light Scattering? The intensity of light they produce is proportional to d6 where d is the particle diameter (ie. I α d6) Aims of this Section............... How can a 3-dimensional object be …
Introduction to Dynamic Light Scattering (DLS) - horiba.com
What is DLS and what does it measure? What is dynamic light scattering? Dynamic light scattering refers to measurement and interpretation of light scattering data on a microsecond …
DYNAMIC LIGHT SCATTERING - University of California, San …
Different methods can be used to study the dynamics of a system with particles in Brownian motion, depending on the time scale of the molecular fluctuations.
2.5.13. Particle size analysis by dynamic light scattering
The method is based on ISO standard 22412:2017 Particle Size Analysis - Dynamic light scattering (DLS). Dynamic light scattering (DLS) can be used to determine the average …
Dynamic Light Scattering
We suspect that this increase was due to multiple scattering as scattered light would pass through more of the sample, thereby increase the probability of a second scattering event. The bead …
A Primer on Particle Sizing Using Dynamic Light Scattering
presents a brief overview of the DLS tech-nique, along with common algorithms used to deconvolute the size distribution from the measured correlation curve. Light scattering is a …
Particle sizing by dynamic light scattering: non-linear …
One such technique, dynamic light scattering (DLS), can be applied to nanometric particles, size from a few nm to about 1 m, which can be suspended in a liquid. DLS has the advantages of …
Static and Dynamic Light Scattering - CMU
Static and dynamic light scattering methods for use in the characterization of dilute solutions of polymers or suspensions of dispersed particles are presented.
Revisiting the method of cumulants for the analysis of …
The method of cumulants is a standard technique used to analyze dynamic light-scattering data mea-sured for polydisperse samples. These data, from an intensity–intensity autocorrelation …
Particle Size Distribution and Zeta Potential Based on …
Size distribution and zeta potential based on dynamic light scattering (DLS) are the essential characterizations prior to any applications of some charged colloids.
Dynamic Light Scattering Distributions by Any Means
Here, we critically discuss the application of DLS for nanoparticle characterization and provide much-needed clarification for ambiguities in the mean-value practice of commercial DLS …
Particle size analysis by dynamic light scattering - PMDA
8 Dynamic light scattering (DLS) can be used to measure the average particle 9 size and the broadness of the size distribution of submicron particles dispersed 10 in a liquid.
A GUIDEBOOK TO PARTICLE SIZE ANALYSIS - Horiba
A light scattering device will average the various dimensions as the particles fl ow randomly through the light beam, producing a distribution of sizes from the smallest to the largest …
NANOCOMPOSIX S GUIDE TO DYNAMIC LIGHT SCATTERING …
Dynamic Light Scattering (DLS) is an important tool for characterizing the size of nanoparticles in solution. DLS measures the light scattered from a laser that passes through a colloidal...
Nanoparticle Analysis: Dynamic Light Scattering for Particle …
What is Dynamic Light Scattering? Dynamic light scattering refers to measurement and interpretation of light scattering data on a microsecond time scale. Static Light Scattering: over …
How to Use Dynamic Light Scattering to Improve the …
Dynamic light scattering (DLS) has become one of the most useful diagnostic tools for crys-tallization. The main purpose of using DLS in crystal screening is to help the investigator under …
8 Advances in dynamic light scattering techniques - Springer
The effect of light scattering by moving particles is technically easier to analyze in the temporal domain when the scattering events are assumed to produce phase shifts rather than …
Characterization of an Unknown Polymer Using Static and …
dynamic light scattering data.1 Static and dynamic light scattering give complementary pieces of information, and for this reason they are commonly used in tandem for characterization of …
Aggregation of Antibody-Drug Conjugates: The Light …
In a separate study, MALS-UV-RI analysis determined drug-antibody ratio (DAR). The light scattering toolkit contributes to complete characterization of ADCs, leading to an efficient and …
WP5004: The diffusion interaction parameter (k as an …
dynamic light scattering (DLS) tools in order to rank the effectiveness of candidates and formulation conditions. DLS can also indicate chemical stability and the average molar mass …
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Dynamic light scattering, also known as photon correlation spectroscopy or quasi-elastic light scattering, is a technique that primarily measures the brownian motion of macromolecules in …
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principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, and on Doppler velocimetry (electrophoretic light scattering, ELS) for zeta potential, most measurements only …
DYNAMIC LIGHT SCATTERING Nanoparticle Size, Charge
All dynamic light scattering measurements use a form of ‘beating’ to strip away the . high optical frequency from the scattered light, leaving the particle motion-induced lower frequencies …
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a. Displays the intensity auto-correlation function (ACF), the raw dynamic light scattering data. b. Displays fit for both cumulants (a single species fit) and regularization (multi-species fit). c. …
DYNAMIC LIGHT SCATTERING - University of Cambridge
The measured data in a dynamic light scattering (DLS) experiment is the correlation curve which should be a smooth, single exponential ... International Standard ISO22412 Particle Size …
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Assessing Nonspecific Interactions with Light Scattering: A2 …
Assessing Nonspecific Interactions with Light Scattering Page 3 of 9 TN9102 Rev B. The Light Scattering Figure of Merit (LS- FOM) measures the contribution to the light scattering signal du …
Dynamic light scattering (DLS) - ResearchGate
DLS for particle size analysis Dynamic light scattering (DLS) is an optical measurement technique for the character- ... data analysis, which will improve the technique’s ability to handle ...
Information-weighted constrained regularization for particle …
Information-weighted constrained regularization for particle size distribution recovery in multiangle dynamic light scattering MIN XU, 1 JIN SHEN,1,* JOHN C. THOMAS,1,2 YU HUANG,1 …
Dynamic light scattering common terms defined
The Z-Average size or Z-Average mean used in dynamic light scattering is a parameter also known as the cumulants mean. It is the primary and ... correlation data (the cumulants …
Particle Light Scattering Methods and Applications
(electrophoretic light scattering) to the characterization of nanoparticles and biotherapeutic drugs. Emphasis will be placed on the analysis of light scattering data and the importance of certain …
A GUIDEBOOK TO PARTICLE SIZE ANALYSIS - Horiba
Static image analysis Dynamic image analysis ... produce the data. Although this approach is simplistic and not perfectly accurate, ... A light scattering device will average the various …
Raynals, an online tool for the analysis of dynamic light …
Dynamic light-scattering (DLS) experiments consist of illuminating a sample with a polarized monochromatic laser and detecting the scattered light with a high temporal reso-lution. The …
Revisiting the method of cumulants for the analysis of …
The method of cumulants is a standard technique used to analyze dynamic light-scattering data mea-sured for polydisperse samples. These data, from an intensity–intensity autocorrelation …
Light Scattering - University of Cincinnati
ideal solutions. In light scattering there are two non-ideal effect — nonideal solutions and large particle size effects. Thus, analysis or deconvolution of light scattering data requires two …
Laboratory Exercise - IUBMB
Protein Analysis by Dynamic Light Scattering: Methods and Techniques for Studentsz Received for publication, May 23, 2012; accepted 23 July 2012 ... avoid several pitfalls during the …
Laboratory Exercise - ResearchGate
Protein Analysis by Dynamic Light Scattering: Methods and Techniques for Studentsz Received for publication, May 23, 2012; accepted 23 July 2012 ... light scattering data, is the product of …
A GUIDEBOOK TO PARTICLE SIZE ANALYSIS - University of Iowa
A light scattering device will average the various dimensions as the particles flow randomly through the light beam, producing a distribution of sizes from the smallest to the largest …
Revisiting the method of cumulants for the analysis of …
The method of cumulants is a standard technique used to analyze dynamic light-scattering data mea-sured for polydisperse samples. These data, from an intensity–intensity autocorrelation …
8 Advances in dynamic light scattering techniques - Springer
8 Advances in dynamic light scattering techniques P. Zakharov, F. Scheffold 8.1 Introduction Recent developments in processing techniques and detection hardware have opened new …
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Analysis of noisy multi-angle dynamic light scattering data
In multi-angle dynamic light scattering measurements, due to the inevitable presence of baseline mea-surement noise, the normalized intensity autocorrelation function (ACF) data deviates …
Fresh Insights into Nanoparticle Characterization: DLS
Interpreting Scattering Data • Scattering data typically cannot be inverted to find particle shape. • We use optical models to interpret data and understand our experiments. • Modern systems …
Application of Static and Dynamic Light Scattering …
CA) was used to collect and process experimental data. Dynamic light scattering analysis Dynamic light scattering measurements were performed on a DynaPro Titan instrument with …
Dynamic light scattering and application to proteins in …
2 DYNAMIC LIGHT SCATTERING THEORY 2.1 SCATTERED LIGHT A tipical set-up for the scattering experiment consist of a laser beam illuminating a sample and a detector set up at …
Dynamic Light Scattering Analysis in Biomedical Research …
Dynamic Light Scattering Analysis in Biomedical Research and Applications of Nanoparticles and Polymers Alexander Knysh, Pavel Sokolov, Igor Nabiev ... affect the calculated data or …
How to Use Dynamic Light Scattering to Improve the …
Dynamic light scattering (DLS) has become one of the most useful diagnostic tools for crys-tallization. The main purpose of using DLS in crystal screening is to help the investigator under …
DYNAMIC LIGHT SCATTERING - ResearchGate
The measured data in a dynamic light scattering (DLS) experiment is the correlation curve which should be a smooth, single exponential decay function for a mono-size particle dispersion.
Dynamic Light Scattering Data Analysis (2024)
Dynamic Light Scattering Data Analysis The Enigmatic Realm of Dynamic Light Scattering Data Analysis: Unleashing the Language is Inner Magic In a fast-paced digital era where …
Revisiting the method of cumulants for the analysis of …
The method of cumulants is a standard technique used to analyze dynamic light-scattering data mea-sured for polydisperse samples. These data, from an intensity–intensity autocorrelation …
Quasielastic Light Scattering for Protein Assembly Studies
data analysis procedures. We explain how the Stokes–Einstein relationship can be used to con- ... Quasielastic light scattering (QLS), also known as dynamic light scattering (DLS), is an optical …
A Practical Minicourse in Dynamic Light Scattering *1
IIb. A Second Solo Experiment: Dynamic Light Scattering Of Dilute Polymer Solutions….including Tips on Preparing Clean Samples, Expected vs. Actual Coherence, and Concentration …
DynaPro Plate Reader III: CMI Getting Started Guide to …
a. Displays the intensity auto-correlation function (ACF), the raw dynamic light scattering data. b. Displays fit for both cumulants (a single species fit) and regularization (multi-species fit). c. …
Selection of Particle Size Standards for Verifying and …
Dynamic light scattering (DLS) is the most accepted and used technique for submicron particle size analysis. Since DLS is a “first principles” technique, systems are not calibrated but …
Laser Light Scattering Dynamic Light Scattering Image …
molecular weight, and surface area analysis. A range of analytical techniques are employed including laser diffraction (Mie Theory), dynamic light scattering, nanoparticle tracking analysis …
Dynamic light scattering (DLS) - AUA
The principles of dynamic light scattering The method of dynamic light scattering (DLS) is the most common measurement technique for particle size analysis in the nanometer range. …
Dynamic Light Scattering - University of California, San Diego
Dynamic Light Scattering - measuring particle-size from the intensityspectrum of scattered light - Biophysical Measurement (Physics 173) - UCSD - Spring 2002 ... For determination of the …
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and undergraduate students w …Protein analysis by dynamic light scattering: methods and...001-2006PDG.pdf - 1 - 1 2 Particle size analysis by dynamic light scattering 3 4 The method is …
AN5007: Characterization of AAV-based viral vectors by …
analysis by SE -MALS. SE -MALS provides detailed analysis, but requires 30 minutes per sample and may not be appropriate for screening of processes and formulations. In contrast, batch …
DLS Sample Preparation - Entegris
With many samples to be analyzed using dynamic light scattering (DLS), the chemist simply pours/pipettes the suspension into a cell, hits go, and a few minutes later has a perfect …
NIR Dynamic Light Scattering for Quantum Dot particle size …
2. Dynamic Light Scattering (DLS) Dynamic Light Scattering (DLS) is a technique based on the measurement of the fluctuation of light scattering through colloidal suspension, in order to get …
Measuring the Size of Nanoparticles in Aqueous Media …
Dynamic Light Scattering NIST-NCL Joint Assay Protocol, PCC-1 Version 1.2 V. A. Hackley J. D. Clogston This publication is available free of charge from: ... and data analysis. This protocol …
Laboratory Exercise - ResearchGate
Protein Analysis by Dynamic Light Scattering: Methods and Techniques for Studentsz Received for publication, May 23, 2012; accepted 23 July 2012 ... light scattering data, is the product of …
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Principles, Methods, and Application of Particle Size Analysis
Jun 20, 2024 · Light scattering • Dynamic light scattering (DLS, measuring fluctuation change of the intensity of the scattered light) • Static light scattering (measuring the total intensity of the …
Dynamic Light Scattering (DLS) - static.igem.org
• Data Processing If characteristics are already known about the sample being measured, this window will allow an appropriate analysis model to be applied and so optimise the …
