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| flow cytometry data analysis: Flow Cytometry Data Analysis James V. Watson, 2005-09-15 This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analyzing dynamic processes from flow cytometry data. These are developed for the analysis of not only individual DNA histograms to obtain the proportion of cells in the cell cycle phases, but also time courses of DNA histograms to yield cell cycle kinetic information; overlapping immunofluorescence distributions with confidence limits for the estimated proportions; enzyme kinetic and membrane transport parameters and a brief introduction to multivariate analysis is given. A distinction is made between data handling, for example gating and counting the numbers of cells within that gate, a process commonly regarded as data analysis but which, in reality, is data handling, and data analysis itself which is the means by which information is extracted. |
| flow cytometry data analysis: Flow Cytometry Data Analysis James V. Watson, 2005-09-15 This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analyzing dynamic processes from flow cytometry data. These are developed for the analysis of not only individual DNA histograms to obtain the proportion of cells in the cell cycle phases, but also time courses of DNA histograms to yield cell cycle kinetic information; overlapping immunofluorescence distributions with confidence limits for the estimated proportions; enzyme kinetic and membrane transport parameters and a brief introduction to multivariate analysis is given. A distinction is made between data handling, for example gating and counting the numbers of cells within that gate, a process commonly regarded as data analysis but which, in reality, is data handling, and data analysis itself which is the means by which information is extracted. |
| flow cytometry data analysis: High-Dimensional Single Cell Analysis Harris G. Fienberg, Garry P. Nolan, 2014-04-22 This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is an invaluable reference book for both basic and clinical researchers. |
| flow cytometry data analysis: Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies Anna Porwit, Marie Christine Béné, 2018-01-25 Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time. |
| flow cytometry data analysis: Practical Flow Cytometry Howard M. Shapiro, 2005-02-25 From the reviews of the 3rd Edition... The standard reference for anyone interested in understandingflow cytometry technology. American Journal of Clinical Oncology ...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating. Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers. |
| flow cytometry data analysis: Flow Cytometry Protocols Teresa S. Hawley, |
| flow cytometry data analysis: Flow Cytometry in Hematopathology Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan, 2003 This unique text offers a systematic and practical approach to the analysis and interpretation of FCM graphics. Using numerous FCM illustrations derived from actual well-documented clinical cases, the authors demonstrate a step-by-step approach to optimal FCM data analysis on specimens suspected of harboring hematopoietic malignancies. The discussion moves from simple to complex specimens, with an emphasis on visual pattern analysis. A wide variety of hematologic disorders are covered, including leukemias and lymphomas. The companion CD-ROM with 80 detailed case studies provides additional opportunities to gain a deeper understanding of FCM data analysis. |
| flow cytometry data analysis: Flow Cytometry Alice Longobardi Givan, 2013-04-10 Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature. |
| flow cytometry data analysis: Flow Cytometry Basics for the Non-Expert Christine Goetz, Christopher Hammerbeck, Jody Bonnevier, 2018-11-08 This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine. |
| flow cytometry data analysis: Flow Cytometry Marion G. Macey, 2007-11-03 Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry. |
| flow cytometry data analysis: Flow Cytometry in Hematopathology Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan, 2002-11-26 Flow cytometry immunophenotyping of hematopoietic disorders is a complex and demanding exercise that requires a good understanding of cell lineages, developmental pathways, and physiological changes, as well as broad experience in hematopathology. The process includes several interrelated stages, from the initial medical decision regarding which hematologic c- dition is appropriate for FCM assay, to the final step of diagnosis whereby the FCM data is correlated with other relevant clinical and laboratory information. The actual FCM testing involves three major steps: pre-analytical (specimen processing, antibody staining), analytical (acquiring data on the flow cytometer) and post-analytical (data analysis and interpretation). The literature, including the latest FCM textbooks, provides ample information on the te- nical principles of FCM such as instrumentation, reagents and laboratory methods, as well as quality control and quality assurance. Similarly, correlations of morphologic findings and p- notypic profiles have been well covered in many publications. In contrast, much less attention has been given to the other equally important aspects of FCM immunophenotyping, especially data analysis. The latter is a crucial step by which a phenotypic profile is established. To bridge this gap in the literature, the focus of this book is more on FCM data analysis than laboratory methods and technical details. For the reader to become familiar with our data analysis strategy, an overview of our approach to the pre-analytical and analytical steps is also presented, with an emphasis on the pre-analytical aspects, which have been rarely touched upon in the literature. |
| flow cytometry data analysis: Flow Cytometry and Cell Sorting Andreas Radbruch, 2013-03-14 The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended. CYTOBIOS |
| flow cytometry data analysis: Imaging Flow Cytometry Natasha S. Barteneva, Ivan A. Vorobjev, 2015-11-23 This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies. |
| flow cytometry data analysis: Immunophenotyping J Philip McCoy Jr, 2019-09-29 This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field. |
| flow cytometry data analysis: Flow Cytometry with Plant Cells Jaroslav Dolezel, Johann Greilhuber, Jan Suda, 2007-06-27 Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists. |
| flow cytometry data analysis: Finding Groups in Data Leonard Kaufman, Peter J. Rousseeuw, 1990-03-22 Partitioning around medoids (Program PAM). Clustering large applications (Program CLARA). Fuzzy analysis (Program FANNY). Agglomerative Nesting (Program AGNES). Divisive analysis (Program DIANA). Monothetic analysis (Program MONA). Appendix. |
| flow cytometry data analysis: Single Cell Analysis J. Paul Robinson, Andrea Cossarizza, 2017-04-18 This book highlights the current state of the art in single cell analysis, an area that involves many fields of science – from clinical hematology, functional analysis and drug screening, to platelet and microparticle analysis, marine biology and fundamental cancer research. This book brings together an eclectic group of current applications, all of which have a significant impact on our current state of knowledge. The authors of these chapters are all pioneering researchers in the field of single cell analysis. The book will not only appeal to those readers more focused on clinical applications, but also those interested in highly technical aspects of the technologies. All of the technologies identified utilize unique applications of photon detection systems. |
| flow cytometry data analysis: Mass Cytometry Helen M McGuire, 2019-08-19 |
| flow cytometry data analysis: Flow Cytometry M. G. Ormerod, David Novo, 2008 Flow cytometry is a technique used to study cells, such as blood cells or cancer cells. It is used in medical and research laboratories. |
| flow cytometry data analysis: Introductory Statistics with R Peter Dalgaard, 2008-06-27 This book provides an elementary-level introduction to R, targeting both non-statistician scientists in various fields and students of statistics. The main mode of presentation is via code examples with liberal commenting of the code and the output, from the computational as well as the statistical viewpoint. Brief sections introduce the statistical methods before they are used. A supplementary R package can be downloaded and contains the data sets. All examples are directly runnable and all graphics in the text are generated from the examples. The statistical methodology covered includes statistical standard distributions, one- and two-sample tests with continuous data, regression analysis, one-and two-way analysis of variance, regression analysis, analysis of tabular data, and sample size calculations. In addition, the last four chapters contain introductions to multiple linear regression analysis, linear models in general, logistic regression, and survival analysis. |
| flow cytometry data analysis: Basic Science Methods for Clinical Researchers Morteza Jalali, Francesca Yvonne Louise Saldanha, Mehdi Jalali, 2017-03-31 Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP) |
| flow cytometry data analysis: Doubled Haploid Production in Crop Plants M. Maluszynski, Kenneth Kasha, B.P. Forster, I. Szarejko, 2013-06-29 The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding programmes. This Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for many crops including some woody tree species. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid populations in numerous crop species, we hope that this Manual will also help molecular biologists in establishing such mapping populations. For many years, both the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) have supported and coordinated research that focuses on development of more efficient doubled haploid production methods and their applications in breeding of new varieties and basic research through their Plant Breeding and Genetics Section of the Joint F AO/IAEA Division of Nuclear Techniques in Food and Agriculture. The first F AO/IAEA scientific network (Coordinated Research Programme - CRP) dealing with doubled haploids was initiated by the Plant Breeding and Genetics Section in 1986. |
| flow cytometry data analysis: Building Bioinformatics Solutions 2nd Edition Conrad Bessant, Darren Oakley, Ian Shadforth, 2014 This book introduces the reader to all the key concepts and technologies needed to begin developing their own bioinformatics tools. The new edition includes more bioinformatics-specific content and a new chapter on good software engineering practices to help people working in teams. |
| flow cytometry data analysis: Skew-Elliptical Distributions and Their Applications Marc G. Genton, 2004-07-27 This book reviews the state-of-the-art advances in skew-elliptical distributions and provides many new developments in a single volume, collecting theoretical results and applications previously scattered throughout the literature. The main goal of this research area is to develop flexible parametric classes of distributions beyond the classical no |
| flow cytometry data analysis: Modern Multidimensional Scaling Ingwer Borg, Patrick Groenen, 2013-04-18 Multidimensional scaling (MDS) is a technique for the analysis of similarity or dissimilarity data on a set of objects. Such data may be intercorrelations of test items, ratings of similarity on political candidates, or trade indices for a set of countries. MDS attempts to model such data as distances among points in a geometric space. The main reason for doing this is that one wants a graphical display of the structure of the data, one that is much easier to understand than an array of numbers and, moreover, one that displays the essential information in the data, smoothing out noise. There are numerous varieties of MDS. Some facets for distinguishing among them are the particular type of geometry into which one wants to map the data, the mapping function, the algorithms used to find an optimal data representation, the treatment of statistical error in the models, or the possibility to represent not just one but several similarity matrices at the same time. Other facets relate to the different purposes for which MDS has been used, to various ways of looking at or interpreting an MDS representation, or to differences in the data required for the particular models. In this book, we give a fairly comprehensive presentation of MDS. For the reader with applied interests only, the first six chapters of Part I should be sufficient. They explain the basic notions of ordinary MDS, with an emphasis on how MDS can be helpful in answering substantive questions. |
| flow cytometry data analysis: Handbook of Flow Cytometry Methods J. Paul Robinson, 1993-03-25 Handbook of Flow Cytometry Methods Edited By J. Paul Robinson Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory manual for investigators involved in research and diagnostic applications of flow cytometry. Handbook of flow cytometry methods offers a collection of proven, basic procedures designed for use in the flow cytometry laboratory. Methods implemented at Purdue University Cytometry Laboratories and developed in laboratories of contributors worldwide provide practical, step-by-step procedures for: the preparation and handling of standards,reagents and specimens laboratory analyses for immunophenotyping measurement of DNA content and cell cycle analyses determination of cell physiology and function set up and operation of flow cytometers computational and data analysis techniques safety precautions Drawing on their collective experience at leading research centers, the editors have fashioned a unique working manual intended to serve as the starting point for the evaluation and establishment of laboratory benchmark standards in flow and image cytometry. Each method is described in detail to be used and modified depending on the user’s needs. Special features of Handbook of Flow Cytometry Methods include: ample space throughout for personal annotations suggestions of alternative procedures; trouble-shooting tips extensive list of reagents and suppliers comb-bound format for ease of use at the lab bench Handbook of Flow Cytometry Methods is an essential laboratory resource for students, faculty, and researchers in cell and molecular biology, immunology, cancer biology, hematology/oncology, and laboratory medicine. |
| flow cytometry data analysis: Basic Principles in Flow Cytometry Vasiliki E. Kalodimou, 2013 |
| flow cytometry data analysis: Computer Aided Intervention and Diagnostics in Clinical and Medical Images J. Dinesh Peter, Steven Lawrence Fernandes, Carlos Eduardo Thomaz, Serestina Viriri, 2019-01-01 This book is a compendium of the ICCMIA 2018 proceedings, which provides an ideal reference for all medical imaging researchers and professionals to explore innovative methods and analyses on imaging technologies for better prospective patient care. This work serves as an exclusive source for new computer assisted clinical and medical developments in imaging diagnosis, intervention and analysis. It includes articles on computer assisted medical scanning techniques, computer-aided diagnosis, robotic surgery and imaging, imaging genomics, clinically-oriented imaging physics and informatics, augmented-reality medical visualization, imaging modalities, computerized radiology, oncology, and surgery. Moreover, information on non-medical imaging that has medical applications such as multi-photon microscopy and confocal, photoacoustic imaging, optical microendoscope, infra-red radiation, and other imaging modalities is also represented. |
| flow cytometry data analysis: Volume 16: How to Detect and Handle Outliers Boris Iglewicz, David C. Hoaglin, 1993-01-08 Outliers are the key focus of this book. The authors concentrate on the practical aspects of dealing with outliers in the forms of data that arise most often in applications: single and multiple samples, linear regression, and factorial experiments. Available only as an E-Book. |
| flow cytometry data analysis: Diagnostic Flow Cytometry in Cytology Pranab Dey, 2021-05-28 The book covers the essential practical techniques of flow cytometry in detail. It is divided into two sections: The first section includes the basic practical techniques of flow cytometry in cytology samples. Chapters under this section provide detailed description of the sampling technique, processing, acquisition of the sample, instrumentation and basic principles of flow cytometry. The second section elucidates clinical applications of flow cytometry. Chapters cover the flow cytometry applications in various haematolymphoid neoplasms, tumors of solid organs and body fluid samples. The flow cytometry findings of different tumors are described with the help of multiple colored cytology microphotographs, flow cytometry graphs, boxes, and tables. In addition, it also describes other ancillary techniques in those neoplastic lesions. The book helps practicing pathologists, technical staff and post graduate students to understand flow cytometry findings of the haematolymphoid neoplasms and solid tumor with special emphasis on cytology along with advanced technique. This book will help the students to interpret flow cytometry graphs. |
| flow cytometry data analysis: Current Protocols on CD-ROM. , 1997 |
| flow cytometry data analysis: Practical Flow Cytometry in Haematology Mike Leach, Mark Drummond, Allyson Doig, Pam McKay, Bob Jackson, Barbara J. Bain, 2015-06-01 The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations. |
| flow cytometry data analysis: Flow Cytogenetics , 2012-12-02 This is the first book to be devoted entirely to the application and development of flow techniques in cytogenetics. It provides comprehensive information on the use of flow cytometry and sorting for chromosome classification and purification. Cytogenetics and molecular biologists will find this book an invaluable reference source. - Practical details for the preparation and analysis of chromosomes using flow cytometry - Flow karyotyping for sensitive rapid analysis of chromosome normality and the detection of aberrant chromosomes - Flow sorting as a source of chromosome-specific DNA for gene mapping and recombinant DNA libraries - Construction and current status of chromosome-specific recombinant DNA libraries |
| flow cytometry data analysis: Flow Cytometry in Microbiology Martin G. Wilkinson, 2015 Flow cytometry is a powerful technique for the rapid analysis of single cells in a mixture. In microbiology, flow cytometry permits the reliable and rapid detection of single or multiple microbes and can provide information about their distribution within cell populations. Flow cytometry may also lead to a faster means of viability counting of microorganisms while, at the same time, enabling a better understanding of all bacterial cells within a given population. Specially adapted commercial cytometers for microbial detection are being developed. In this text, renowned contributors have brought together a wealth of experience demonstrating the power and limitations of flow cytometry as it currently stands in the field of microbiology. The book commences with an overview of flow cytometry by Professor Howard Shapiro, one of the most eminent scientists in the area of flow cytometry. Further chapters discuss: cytometry technology and applications in environmental biotechnology * microbial community fingerprinting * clinical microbiology * lactic acid bacteria * spore forming bacteria * yeasts and fungi * water analysis * chip-based cytometry assays. The final chapter describes the adoption of flow cytometry to routine water quality analysis in Switzerland and provides a fascinating case study of how this emerging microbial technology overcame technical, regulatory, and practical issues to become a standard rapid quality control methodology. The book provides a thorough description of flow cytometry and includes practical and up-to-date information aimed specifically at microbiologists. *** Librarians: ebook available on ProQuest and EBSCO [Subject: Microbiology, Life Science] |
| flow cytometry data analysis: Flow Cytometry in Evaluation of Hematopoietic Neoplasms Sindhu Cherian, Brent Wood, 2012 Flow Cytometry in Evaluation of Hematopoietic Neoplasms: A Case-Based Approach is a practical, case-based guide to flow cytometric analysis in the workup of hematopoietic neoplasms presenting in the peripheral blood, marrow, lymphoid tissue, and extranodal sites. Using multi-color techniques pioneered by Brent Wood, the text demonstrates a unique approach to diagnosis of hematopoietic malignancies as well as identification of small abnormal populations in the posttherapy setting (minimal residual disease testing). The publication contains an introduction to immunophenotypic changes seen in normal hematopoiesis along with an overview of the evaluation of lymphomas, leukemias, and myeloid stem- cell neoplasms. These concepts are further illustrated by a series of 36 cases, each dedicated to a specific disease entity. Each case provides detailed, full-color images of flow cytometric dot plots that clearly outline the features of the disease, accompanied by a clinical history and thorough discussion, enabling readers to develop and work through a differential diagnosis and recognize potential diagnostic pitfalls. Take-home points for each case emphasize critical concepts in flow cytometric diagnosis. Flow Cytometry in Evaluation of Hematopoietic Neoplasms provides pathologists, residents, laboratory technologists, and hematologists with both a study guide and an atlas for regular consultation in the clinical flow cytometry laboratory. The 2008 World Health Organization (WHO) classification system, French-American-British (FAB) classifications, and 2006 Bethesda Consensus recommendations are incorporated in the text. |
| flow cytometry data analysis: Cytometry, Part A , 2000-10-31 Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area. - Contains 56 extensive chapters authored by world authorities on cytometry - Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases - Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine - Complements the first and second editions on flow cytometry in the Methods in Cell Biology series and includes new sections on technology principles |
| flow cytometry data analysis: Introduction to Flow Cytometry Jakub Werner, 2020-02-26 This book first discusses the general principles of flow cytometry. This technique continues to be developed and is used in many medical applications. The authors discuss the condition of cell suspension which is entrained in the centre of stream of liquid. Additionally, the most common usage and selected applications of flow cytometry in clinical practice is presented. In recent years, thanks to the use of new generation dyes, the cytometry has a much higher sensitivity and specificity and allows for the simultaneous registration of more parameters, which leads to a huge amount of information from a single experiment. Selected techniques of flow cytometry dedicated to measuring DNA content are reviewed. Flow cytometry is used to estimate DNA content in individual cells in large cell populations. Flow cytometry measures changes in the quality and quantity of specific cells. As such, flow cytometer-associated software for analysis of large data sets is examined. Parameters and probes used in this technique are also discussed. Next, the authors discuss the application of flow cytometry in the study of cells in normal blood and bone marrow. The application of flow cytometry to acute leukaemia diagnosis is explored. This diagnostic method is prerequisite for individual treatment strategies and for the evaluation of treatment response. Following this, the application of flow cytometry to disorders of plasma cell diagnosis is discussed. This compilation similarly explores the evolution of the crossmatch assay and the important factors to take into consideration while performing, as well as interpreting results of this fundamental assay for the fate of the transplanted organ. The penultimate chapter mainly focuses on comparing cytometric bead array to ELISA, which is considered the gold standard for soluble molecules determination. In closing, the authors discuss modern applications of flow cytometry, including the analysis of tumour cells, tumour infiltrating leukocytes, untouched isolation of tumour cells, exosome isolation and analysis, circulating tumour cells, and GMP-engineered T cells. |
| flow cytometry data analysis: Lipid Nanocarriers for Drug Targeting Alexandru Mihai Grumezescu, 2018-04-16 Lipid Nanocarriers for Drug Targeting presents recent advances in the area of lipid nanocarriers. The book focuses on cationic lipid nanocarriers, solid lipid nanocarriers, liposomes, thermosensitive vesicles, and cubosomes, with applications in phototherapy, cosmetic and others. As the first book related to lipid nanocarriers and their direct implication in pharmaceutical nanotechnology, this important reference resource is ideal for biomaterials scientists and those working in the medical and pharmaceutical industries that want to learn more on how lipids can be used to create more effective drug delivery systems. - Highlights the most commonly used types of lipid nanocarriers and explains how they are applied in pharmacy - Shows how lipid nanocarriers are used in different types of treatment, including oral medicine, skin repair and cancer treatment - Assesses the pros and cons of using different lipid nanocarriers for different therapies |
| flow cytometry data analysis: Flow Cytometry Marvin A. Van Dilla, 1985 |
| flow cytometry data analysis: Molecular Data Analysis Using R Csaba Ortutay, Zsuzsanna Ortutay, 2016-12-29 This book addresses the difficulties experienced by wet lab researchers with the statistical analysis of molecular biology related data. The authors explain how to use R and Bioconductor for the analysis of experimental data in the field of molecular biology. The content is based upon two university courses for bioinformatics and experimental biology students (Biological Data Analysis with R and High-throughput Data Analysis with R). The material is divided into chapters based upon the experimental methods used in the laboratories. Key features include: • Broad appeal--the authors target their material to researchers in several levels, ensuring that the basics are always covered. • First book to explain how to use R and Bioconductor for the analysis of several types of experimental data in the field of molecular biology. • Focuses on R and Bioconductor, which are widely used for data analysis. One great benefit of R and Bioconductor is that there is a vast user community and very active discussion in place, in addition to the practice of sharing codes. Further, R is the platform for implementing new analysis approaches, therefore novel methods are available early for R users. |
Chapter 4: Data Analysis | Flow Cytometry - A Basic Introduction
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How to Interpret Flow Cytometry Results | KCAS Bio
Feb 26, 2022 · In this blog we explore the important of flow cytometry data analysis and how the need to understand increasing complex data sets has driven the development of new …
Deans CoREs - Flow Cytometry | Icahn School of Medicine
The Flow Cytometry CoRE offers users access to FlowJo, a powerful cytometry data analysis software. This site-licensed tool provides premium analysis features, report generation, and …
Chapter 4: Data Analysis | Flow Cytometry - A Basic Introduction
While flow cytometry generally gives the percentage of a particular sub-set of cells, some flow cytometers precisely record the the volume of sample analysed or deliver a fixed volume of …
Basic Data Analysis, Gating, and Statistics in Flow Cytometry
•DNA Cell Cycle analysis •Proliferation analysis •Absolute counts •Volumetric based acquisition cytometer or counting beads spiked in sample at known concentration. (Counting beads tend …
Data analysis in flow cytometry - Abcam
Here we cover signal measurement, visualizing multi-color flow cytometry data and gating strategies to quantify your data.
Data-Driven Flow Cytometry Analysis - PMC - PubMed Central …
The emergence of flow and mass cytometry technologies capable of generating 40-dimensional data has spurred research into automated methodologies that address bottlenecks across the …
How to Interpret Flow Cytometry Data | Fortis Life Sciences
Flow cytometry data is typically depicted in one of two formats: a histogram or a scatter plot. Histograms are used to graphically present single-parameter data. This type of data …
Spectral Flow Cytometry Data Analysis - Thermo Fisher Scientific
Learn more about spectral flow cytometry data analysis including data generation, visualization, and data mining.
Flow cytometry data analysis: Recent tools and algorithms
Flow cytometry (FCM) allows scientists to rapidly quantify up to 50 parameters for millions of cells per sample.
Quick Tips on Flow Cytometry Data Analysis - BD Biosciences
Feb 12, 2024 · Watch the video below to see how you can adjust your data display to improve flow cytometry data analysis.
How to Interpret Flow Cytometry Results | KCAS Bio
Feb 26, 2022 · In this blog we explore the important of flow cytometry data analysis and how the need to understand increasing complex data sets has driven the development of new …
Deans CoREs - Flow Cytometry | Icahn School of Medicine
The Flow Cytometry CoRE offers users access to FlowJo, a powerful cytometry data analysis software. This site-licensed tool provides premium analysis features, report generation, and …
