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| fragment analysis by capillary electrophoresis: Capillary Gel Electrophoresis Andras Guttman, László Hajba, 2021-12-04 Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses |
| fragment analysis by capillary electrophoresis: Capillary Electrophoresis Technology Norberto A. Guzman, 1993-08-10 Tracing the sequence of observations that has led to current understanding in the field, this reference presents the basic concepts, instrumentation and applications of capillary electrophoresis, examining its many features such as high-power resolution, high-mass sensitivity, overall sensitivity and low-sample volume requirements. This work highlights the use of capillary elctrophoresis for the identification, separation, detection and characterization of substances on the molecular counting level. Illustrating the major technical maneuvers for common operations and applications, Capillary Electrophoresis Technology outlines the theoretical concepts and mathematical expressions of capillary electrophoresis; describes advances in instrumentation hardware and detection systems. It explains the advantages and limitations of the different variants of capillary electrophoresis; and provides extra coverage of areas in which capillary electrophoresis has grown increasingly popular, including the identification and characterization of small molecules and macromolecules. Written by experts in the field, this book is aimed at analytical and clinical chemists and biochemists, chemical engineers, biologists, pharmacists, biotechnologists and students in these disciplines. |
| fragment analysis by capillary electrophoresis: Capillary Electrophoresis Paul D. Grossman, Joel C. Colburn, 2012-12-02 This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations.This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:Electroosmosis, separation efficiency, and Joule heatingDetection methodsIn-depth discussion of the separation principles and capabilities of the major modes of CESieving gel electrophoresisIsoelectric focusingFree solution CEMicellar electrokinetic capillary chromatographyEntangled polymer matrix-based separationDetailed treatment of the application of CE to a wide range of molecules, supplemented with extensive hands-on illustrations |
| fragment analysis by capillary electrophoresis: Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das, 2021-02-21 The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples. It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples. Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications. |
| fragment analysis by capillary electrophoresis: Analysis of Nucleic Acids by Capillary Electrophoresis Christoph Heller, 2013-06-29 Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids. |
| fragment analysis by capillary electrophoresis: The FBI DNA Laboratory CreateSpace Independent Publishing Platform, Office of the Investigator General, 2018-07-09 The FBI DNA laboratory : a review of protocol and practice vulnerabilities. |
| fragment analysis by capillary electrophoresis: Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis Bin Zhuang, 2017-07-20 This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice. |
| fragment analysis by capillary electrophoresis: Bacterial Artificial Chromosomes Shaying Zhao, Marvin Stodolsky, 2004 |
| fragment analysis by capillary electrophoresis: Epigenomics Krishnarao Appasani, 2012-08-02 Understanding mechanisms of gene regulation that are independent of the DNA sequence itself - epigenetics - has the potential to overthrow long-held views on central topics in biology, such as the biology of disease or the evolution of species. High throughput technologies reveal epigenetic mechanisms at a genome-wide level, giving rise to epigenomics as a new discipline with a distinct set of research questions and methods. Leading experts from academia, the biotechnology and pharmaceutical industries explain the role of epigenomics in a wide range of contexts, covering basic chromatin biology, imprinting at a genome-wide level, and epigenomics in disease biology and epidemiology. Details on assays and sequencing technology serve as an up-to-date overview of the available technological tool kit. A reliable guide for newcomers to the field as well as experienced scientists, this is a unique resource for anyone interested in applying the power of twenty-first-century genomics to epigenetic studies. |
| fragment analysis by capillary electrophoresis: Molecular Biomethods Handbook John M. Walker, Ralph Rapley, 2008-11-04 Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs. |
| fragment analysis by capillary electrophoresis: Separation Techniques Applied to Omics Sciences Ana Valéria Colnaghi Simionato, 2021-10-09 This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one. |
| fragment analysis by capillary electrophoresis: The Nucleic Acid Protocols Handbook Ralph Rapley, 2008-06-29 A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field. |
| fragment analysis by capillary electrophoresis: Capillary Electrophoresis of Nucleic Acids Keith R. Mitchelson, Jing Cheng, 2008-02-05 The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications. |
| fragment analysis by capillary electrophoresis: Antibody Glycosylation Marija Pezer, 2021-10-22 This book summarizes recent advances in antibody glycosylation research. Covering major topics relevant for immunoglobulin glycosylation - analytical methods, biosynthesis and regulation, modulation of effector functions - it provides new perspectives for research and development in the field of therapeutic antibodies, biomarkers, vaccinations, and immunotherapy. Glycans attached to both variable and constant regions of antibodies are known to affect the antibody conformation, stability, and effector functions. Although it focuses on immunoglobulin G (IgG), the most explored antibody in this context, and unravels the natural phenomena resulting from the mixture of IgG glycovariants present in the human body, the book also discusses other classes of human immunoglobulins, as well as immunoglobulins produced in other species and production systems. Further, it reviews the glycoanalytical methods applied to antibodies and addresses a range of less commonly explored topics, such as automatization and bioinformatics aspects of high-throughput antibody glycosylation analysis. Lastly, the book highlights application areas ranging from the ones already benefitting from antibody glycoengineering (such as monoclonal antibody production), to those still in the research stages (such as exploration of antibody glycosylation as a clinical or biological age biomarker), and the potential use of antibody glycosylation in the optimization of vaccine production and immunization protocols. Summarizing the current knowledge on the broad topic of antibody glycosylation and its therapeutic and biomarker potential, this book will appeal to a wide biomedical readership in academia and industry alike. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. |
| fragment analysis by capillary electrophoresis: Forensic DNA Typing John M. Butler, 2005-02-08 Forensic DNA Typing, Second Edition, is the only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome. It examines the science of current forensic DNA typing methods by focusing on the biology, technology, and genetic interpretation of short tandem repeat (STR) markers, which encompass the most common forensic DNA analysis methods used today. The book covers topics from introductory level right up to cutting edge research. High-profile cases are addressed throughout the text, near the sections dealing with the science or issues behind these cases. Ten new chapters have been added to accommodate the explosion of new information since the turn of the century. These additional chapters cover statistical genetic analysis of DNA data, an emerging field of interest to DNA research. Several chapters on statistical analysis of short tandem repeat (STR) typing data have been contributed by Dr. George Carmody, a well-respected professor in forensic genetics. Specific examples make the concepts of population genetics more understandable. This book will be of interest to researchers and practitioners in forensic DNA analysis, forensic scientists, population geneticists, military and private and public forensic laboratories (for identifying individuals through remains), and students of forensic science. *The only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome*Chapters cover the topic from introductory level right up to cutting edge research*High-profile cases are addressed throughout the book, near the sections dealing with the science or issues behind these cases*NEW TO THIS EDITION: D.N.A. Boxes--boxed Data, Notes & Applications sections throughout the book offer higher levels of detail on specific questions |
| fragment analysis by capillary electrophoresis: Clinical Applications of Capillary Electrophoresis Terry M. Phillips, Heather Kalish, 2012-09-13 Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used. Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science. Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research. |
| fragment analysis by capillary electrophoresis: Biotechnologies for Plant Mutation Breeding Joanna Jankowicz-Cieslak, Thomas H. Tai, Jochen Kumlehn, Bradley J. Till, 2016-12-08 This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species. |
| fragment analysis by capillary electrophoresis: Mutation Detection R. G. H. Cotton, E. Edkins, S. Forrest, 1998-02-05 Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnbosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field. |
| fragment analysis by capillary electrophoresis: Microsatellites S. K. Kantartzi, 2013 Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology[trade mark symbol] series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis -- page 4 of cover. |
| fragment analysis by capillary electrophoresis: Handbook of Capillary Electrophoresis, Second Edition James P. Landers, 1996-12-23 Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science. |
| fragment analysis by capillary electrophoresis: Molecular Diagnostics William B. Coleman, Gregory J. Tsongalis, 2007-10-28 Accompanying CD-ROM contains ... a companion eBook version of Molecular diagnostics : for the clinical laboratorian, Second edition ... for downloading and use in the reader's PC or PDA.--Page 4 of cover. |
| fragment analysis by capillary electrophoresis: Capillary Electrophoresis–Mass Spectrometry for Metabolomics Rawi Ramautar, 2018-07-13 Capillary electrophoresis–mass spectrometry (CE-MS) has become a very useful analytical technique for the profiling of highly polar and charged metabolites in biological samples. In this book, the unique features of CE-MS for metabolomics studies are highlighted including CE separation modes, capillary coatings and practical aspects of CE-MS coupling alongside a comprehensive overview of recent technological developments and applications. CE-MS can be considered a relatively new technique in the field of metabolomics and it is therefore important to inform the scientific community about the possibilities of advanced CE-MS approaches for metabolomics studies. This book outlines the potential of this technique for researchers working in metabolomics, bioanalytics and biomarker analysis. |
| fragment analysis by capillary electrophoresis: DNA Technology in Forensic Science National Research Council, Division on Earth and Life Studies, Commission on Life Sciences, Committee on DNA Technology in Forensic Science, 1992-02-01 Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students. |
| fragment analysis by capillary electrophoresis: Electrophoresis in Practice Reiner Westermeier, 2016-05-16 Electrophoresis in Practice ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese. |
| fragment analysis by capillary electrophoresis: Forensic DNA Evidence Interpretation John S. Buckleton, Jo-Anne Bright, Duncan Taylor, 2016-04-21 Now in its second edition, Forensic DNA Evidence Interpretation is the most comprehensive resource for DNA casework available today. Written by leaders in the fields of biology and statistics, including a contribution from Peter Gill, the father of DNA analysis, the book emphasizes the interpretation of test results and provides the necessary formulae in an easily accessible manner. This latest edition is fully updated and includes current and emerging techniques in this fast-moving field. The book begins by reviewing all pertinent biology, and then provides information on every aspect of DNA analysis. This includes modern interpretation methods and contemporary population genetic models available for estimating DNA frequencies or likelihood ratios. Following a chapter on procedures for validating databases, the text presents overviews and performance assessments of both modern sampling uncertainty methods and current paternity testing techniques, including new guidelines on paternity testing in alignment with the International Society for Forensic Genetics. Later chapters discuss the latest methods for mixture analysis, LCN (ultra trace) analysis and non-autosomal (mito, X, and Y) DNA analysis. The text concludes with an overview of procedures for disaster victim identification and information on DNA intelligence databases. Highlights of the second edition include: New information about PCR processes, heterozygote balance and back and forward stuttering New information on the interpretation of low template DNA, drop models and continuous models Additional coverage of lineage marker subpopulation effects, mixtures and combinations with autosomal markers This authoritative book provides a link among the biological, forensic, and interpretative domains of the DNA profiling field. It continues to serve as an invaluable resource that allows forensic scientists, technicians, molecular biologists and attorneys to use forensic DNA evidence to its greatest potential. |
| fragment analysis by capillary electrophoresis: Capillary Electrophoresis: Principles and Practice Reinhard Kuhn, Sabrina Hoffstetter-Kuhn, 2013-03-07 Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. This method gives new information about the investigated substances which can- not easily be obtained by other means. CE has become an established method only recently, but will be implemented in almost every analytical laboratory in industry, service units and academia in the near future. The most important fields of CE application are pharmaceutical and biochemical research and quality control. The authors have exhaustive practical experience in the application of CE methods in the pharmaceutical industry and provide the reader with a comprehensive treatment of this method. The main focus is on how to solve problems when applying CE in the laboratory. Physico-chemical theory is only dealt with in depth when necessary to understand the underlying separation mechanisms in order to solve your problems at the analytical bench. An addendum includes tables on the preparation of buffers and recommended further reading. |
| fragment analysis by capillary electrophoresis: Forensic DNA Typing: Principles, Applications and Advancements Pankaj Shrivastava, Hirak Ranjan Dash, Jose A. Lorente, Jahangir Imam, 2020-12-11 The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis. Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis. Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science. Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics. The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping. Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level. |
| fragment analysis by capillary electrophoresis: Capillary Zone Electrophoresis František Foret, Ludmila Křivánková, Petr Boček, 1993 |
| fragment analysis by capillary electrophoresis: Medical BioMethods Handbook John M. Walker, Ralph Rapley, 2005-03-18 John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy. |
| fragment analysis by capillary electrophoresis: Forensic DNA Typing Protocols Angel Carracedo, 2008-02-02 A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs. |
| fragment analysis by capillary electrophoresis: Protein Electrophoresis in Clinical Diagnosis David Keren, 2003-09-26 Since the publication of High-Resolution Electrophorsesis and Immunofixation 2e, there have been ever-increasing advances in the analyses of proteins, by electrophoresis in particular. Protein Electrophoresis in Clinical Diagnosis shows the changes in both techniques and interpretation, presenting a comprehensive review of serum protein techniques, |
| fragment analysis by capillary electrophoresis: RNA Methodologies Robert E. Farrell Jr., 2010-07-22 This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects |
| fragment analysis by capillary electrophoresis: Genotyping Ibrokhim Y. Abdurakhmonov, 2018 A rare norovirus (NoV) genotype GII.17 has recently emerged and rapidly became predominant in most East Asian countries in the winters of 2014-2015. In this study, we report the diversity of NoV GII.17 in detail; a total of 646 GII.17 sequences obtained during 1978-2015 were analyzed and subjected to meta-analysis. At least five major recombinant GII.17 clusters were identified. Each recombinant variant group appeared to have emerged following the time order: GII.P4-GII.17 (1978-1990), GII.P16-GII.17 (2001-2004), GII.P13-GII.17 (2004-2010), GII.Pe-GII.17 (2012-2015) and GII.P3-GII.17 (2011-2015). The newly emerged GII.P3-GII.17 variant, which exhibited significant sequence and structure variations, is evolving toward a unique lineage. Our results indicate that circulation of GII.17 appears to change every 3-5 years due to replacement by a newly emerged variant and that the evolution of GII.17 is sequentially promoted by inter-genotype recombination, which contributes to the exchange between non-GII.17 and GII.17 RdRp genes and drives the evolution of GII.17 capsid genes. |
| fragment analysis by capillary electrophoresis: Essentials of Glycobiology Ajit Varki, Maarten J. Chrispeels, 1999 Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. Essentials of Glycobiology describes their biogenesis and function and offers a useful gateway to the understanding of glycans. |
| fragment analysis by capillary electrophoresis: Practical Capillary Electrophoresis Robert Weinberger, 2000-04-18 In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. - Illustrated with detailed diagrams of electrophoretic phenomena - Offers step-by-step methods development schemes - Presents techniques for developing quantitative, robust, and precise methods - Includes an extensive troubleshooting guide - Updates and greatly expands on the first edition-more than 50% of the text is new - Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE |
| fragment analysis by capillary electrophoresis: Gel Electrophoresis: Nucleic Acids Dr Robin Martin, 2020-08-26 Through its clear presentation of the basic concepts, Gel Electrophoresis: Nucleic Acids breaks new ground by describing the principles of the technique without resorting to complicated protocols and recipes. |
| fragment analysis by capillary electrophoresis: Handbook of Capillary Electrophoresis Applications H. Shintani, 2012-12-06 Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science. |
| fragment analysis by capillary electrophoresis: Capillary Electromigration Separation Methods Colin F. Poole, 2018-04-13 Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins. - Edited and authored by world-leading capillary electrophoresis experts - Presents comprehensive coverage on the subject - Includes extensive referencing that facilitates the identification of key research developments - Provides more than 50 figures and tables that aid in the retention of key concepts |
| fragment analysis by capillary electrophoresis: Principles and Technical Aspects of PCR Amplification Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays, 2008-03-14 Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its discovery, multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book). |
| fragment analysis by capillary electrophoresis: Prenatal Diagnosis Brynn Levy, 2018-12-01 This second edition volume expands on the first edition with more detailed methodologies on prenatal testing and diagnosis, and also covers next-generation sequencing techniques. The chapters in this book are divided into three sections: preimplantation genetic testing, traditional prenatal testing, and non-invasive prenatal testing. This book covers topics such as molecular testing for preimplantation genetic diagnosis of single gene disorders; DNA extraction from various types of prenatal specimens; prenatal diagnosis of cystic fibrosis and Tay-Sachs disease; chromosomal SNP microarrays; and isolation of cell-free DNA from maternal plasma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Prenatal Diagnosis, Second Edition is a valuable resource for any researcher interested in reproducing these techniques in their clinical laboratories. |
DNA FRAGMENT ANALYSIS - University of Northern British …
DNA Fragment Analysis by Capillary Electrophoresis 7 Contents Understanding spatial calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Chapter 18 Capillary Electrophoresi s with Applied ... - Springer
capillary electrophoresis system used to perform fragment analysis of forensic samples using either an 8-capillary array (3500) or a 24-capillary array (3500xL) [1]. Polymer fills each of the …
Fragment Analysis By Capillary Electrophoresis
Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation …
DNA Sequencing and Fragment Analysis by Capillary …
Fluorescent DNA fragment analysis is one of the most useful methods in molecular biology. The method measures the relative size of DNA fragments with a very high resolution and …
Fragment analysis applications guide - Thermo Fisher Scientific
Apr 29, 2009 · In the following chapters, we provide thorough coverage of the principles of fragment analysis technologies, the basics of multi-color capillary electrophoresis, the steps in …
Nucleic Acid Analysis for Sample Quality Assessment Using …
The Fragment Analyzer systems utilize automated parallel capillary electrophoresis to provide reliable QC for a broad variety of sample types and applications, including NGS libraries, RNA …
DNA Fragment Analysis by Capillary Electrophoresis - LSU
DNA Fragment Analysis by Capillary Electrophoresis Publication Number 4474504 Revision B For Research Use Only. Not intended for use in diagnostic procedures. USER GUIDE
Fragment Analysis FAQ - biotech.ufl.edu
Fragment Analysis is the process of labelling DNA fragments with fluorescent dyes and separating the fragments by size using capillary electrophoresis. Multiple dyes can be used to label …
Analysis of double-stranded DNA fragments using capillary …
In this application note, we demonstrate how the Applied BiosystemsTM genetic analyzer family of CE instruments can be used for evaluating the quality of dsDNA samples. The simple …
Fragment Analysis By Capillary Electrophoresis Copy
Fragment Analysis By Capillary Electrophoresis: Capillary Electrophoresis of Nucleic Acids Keith R. Mitchelson,Jing Cheng,2008-02-05 The development of PCR which enables extremely …
D NA fragment analysis by capillary electrophoresis
fragment analysis can provide sizing, relative quantitation, and genotyping information for fluorescently labeled DNA fragments produced by PCR using primers designed for a
Fragment Analysis - Frequently Asked Questions - University …
Q: What is Fragment Analysis? A: Fragment Analysis is the process of labelling DNA fragments with fluorescent dyes and separating the fragments by size using capillary electrophoresis.
Fragment Analysis By Capillary Electrophoresis (book)
Fragment Analysis By Capillary Electrophoresis: Capillary Electrophoresis of Nucleic Acids Keith R. Mitchelson,Jing Cheng,2008-02-05 The development of PCR which enables extremely …
Multiplex detection of respiratory pathogens using fragment …
fragment analysis by capillary electrophoresis (CE) can be used for concurrent detection of several respiratory RNA viruses in a single sample, with high sensitivity and specificity • The …
Sample Quality Control in Next-Generation Sequencing …
The Agilent Fragment Analyzer systems use automated parallel capillary electrophoresis to provide reliable quality control (QC) of nucleic acids for various applications, including next …
Fragment Analysis By Capillary Electrophoresis (2024)
concepts and mathematical expressions of capillary electrophoresis describes advances in instrumentation hardware and detection systems It explains the advantages and limitations of …
Fragment Analysis by capillary electrophoresis
Fragment Analysis by capillary electrophoresis Technique used in genetics to separate and size fluorescently labeled DNA fragments. This fragment analysis 4-step workflow is …
DNA Fragment Analysis by Capillary Electrophoresis
DNA Fragment Analysis by Capillary Electrophoresis 7 Contents CHAPTER 5 Data Analysis with GeneMapper® Software and Peak Scanner™ Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . …
DNA FRAGMENT ANALYSIS - University of Northern British …
DNA Fragment Analysis by Capillary Electrophoresis 7 Contents Understanding spatial calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Capillary Electrophoresis of DNA - University of Michigan
Capillary electrophoresis (CE), an alternative to conventional slab gel electrophoresis, has developed over the past few years into a very powerful tool for the separation of DNA fragments.
Chapter 18 Capillary Electrophoresi s with Applied ... - Springer
capillary electrophoresis system used to perform fragment analysis of forensic samples using either an 8-capillary array (3500) or a 24-capillary array (3500xL) [1]. Polymer fills each of the …
Fragment Analysis By Capillary Electrophoresis
Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation …
DNA Sequencing and Fragment Analysis by Capillary …
Fluorescent DNA fragment analysis is one of the most useful methods in molecular biology. The method measures the relative size of DNA fragments with a very high resolution and …
Fragment analysis applications guide - Thermo Fisher Scientific
Apr 29, 2009 · In the following chapters, we provide thorough coverage of the principles of fragment analysis technologies, the basics of multi-color capillary electrophoresis, the steps in …
Nucleic Acid Analysis for Sample Quality Assessment Using …
The Fragment Analyzer systems utilize automated parallel capillary electrophoresis to provide reliable QC for a broad variety of sample types and applications, including NGS libraries, RNA …
DNA Fragment Analysis by Capillary Electrophoresis - LSU
DNA Fragment Analysis by Capillary Electrophoresis Publication Number 4474504 Revision B For Research Use Only. Not intended for use in diagnostic procedures. USER GUIDE
Fragment Analysis FAQ - biotech.ufl.edu
Fragment Analysis is the process of labelling DNA fragments with fluorescent dyes and separating the fragments by size using capillary electrophoresis. Multiple dyes can be used to label …
Analysis of double-stranded DNA fragments using capillary …
In this application note, we demonstrate how the Applied BiosystemsTM genetic analyzer family of CE instruments can be used for evaluating the quality of dsDNA samples. The simple fragment …
Fragment Analysis By Capillary Electrophoresis Copy
Fragment Analysis By Capillary Electrophoresis: Capillary Electrophoresis of Nucleic Acids Keith R. Mitchelson,Jing Cheng,2008-02-05 The development of PCR which enables extremely …
D NA fragment analysis by capillary electrophoresis
fragment analysis can provide sizing, relative quantitation, and genotyping information for fluorescently labeled DNA fragments produced by PCR using primers designed for a
Fragment Analysis - Frequently Asked Questions - University …
Q: What is Fragment Analysis? A: Fragment Analysis is the process of labelling DNA fragments with fluorescent dyes and separating the fragments by size using capillary electrophoresis.
Fragment Analysis By Capillary Electrophoresis (book)
Fragment Analysis By Capillary Electrophoresis: Capillary Electrophoresis of Nucleic Acids Keith R. Mitchelson,Jing Cheng,2008-02-05 The development of PCR which enables extremely …
Multiplex detection of respiratory pathogens using fragment …
fragment analysis by capillary electrophoresis (CE) can be used for concurrent detection of several respiratory RNA viruses in a single sample, with high sensitivity and specificity • The …
Sample Quality Control in Next-Generation Sequencing …
The Agilent Fragment Analyzer systems use automated parallel capillary electrophoresis to provide reliable quality control (QC) of nucleic acids for various applications, including next …
Fragment Analysis By Capillary Electrophoresis (2024)
concepts and mathematical expressions of capillary electrophoresis describes advances in instrumentation hardware and detection systems It explains the advantages and limitations of …
Fragment Analysis by capillary electrophoresis
Fragment Analysis by capillary electrophoresis Technique used in genetics to separate and size fluorescently labeled DNA fragments. This fragment analysis 4-step workflow is …
