Advertisement
| fxcycle pi/rnase staining solution: Unveiling the Impact of Local or Systemic Therapeutic Strategies on the Tumor Microenvironment Jian Huang, Chao Ni, Liu Yang, Kevin J. Ni, Hengyu Li, 2022-02-14 |
| fxcycle pi/rnase staining solution: Hepatocytes Naoki Tanimizu, 2022-09-20 This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepatocellular carcinoma subtypes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Hepatocytes: Methods and Protocols aims to provide techniques to further understand molecular mechanisms involved in the regulating hepatocyte functions and underlying liver diseases. |
| fxcycle pi/rnase staining solution: Mediterranean Diet and Cancer: Experimental and Epidemiological Perspectives Wamidh H. Talib, Eyad Elkord, Mostafa Waly, Lina Tareq Al Kury, 2022-11-28 |
| fxcycle pi/rnase staining solution: Host-pathogen crosstalk: implications in host cellular processes by intracellular pathogens Rajni Garg, Deeksha Tripathi , Saurabh Mishra, Imtiyaz Yaseen, 2024-11-06 Infectious diseases are still leading cause of mortality around the world. Of particular importance in this regard are the intracellular pathogens, which dwell inside the host and manipulate host’s machinery for their growth. These include pathogens like Brucella abortus, Listeria monocytogenes, Chlamydia trachomatis, Coxiella burnetii, Mycobacterium tuberculosis, Salmonella enterica, etc. These pathogens have evolved multiple mechanisms to survive in intracellular niche. The host in turn tries to reprogram its activities to combat their growth. This host-pathogen co-evolution warrants detailed study to probe the ever-changing virulence repertoire of the pathogen, that can be used for pathogen targeting. Through this topic, we would like to explore the lesser explored area of host pathogen interactions. Studying host-pathogen crosstalk at their interface will help us in understanding this complex biology and might prove beneficial in development of new therapeutic targets. Understanding the holistics of host-pathogen interaction, will pave way for development of host-directed therapies. |
| fxcycle pi/rnase staining solution: Basic Clinical Radiobiology Michael C. Joiner, Albert J. van der Kogel, 2018-08-28 Basic Clinical Radiobiology is a concise but comprehensive textbook setting out the essentials of the science and clinical application of radiobiology for those seeking accreditation in radiation oncology, clinical radiation physics, and radiation technology. Fully revised and updated to keep abreast of current developments in radiation biology and radiation oncology, this fifth edition continues to present in an interesting way the biological basis of radiation therapy, discussing the basic principles and significant developments that underlie the latest attempts to improve the radiotherapeutic management of cancer. This new edition is highly illustrated with attractive 2-colour presentation and now includes new chapters on stem cells, tissue response and the convergence of radiotherapy, radiobiology, and physics. It will be invaluable for FRCR (clinical oncology) and equivalent candidates, SpRs (and equivalent) in radiation oncology, practicing radiation oncologists and radiotherapists, as well as radiobiologists and radiotherapy physicists. |
| fxcycle pi/rnase staining solution: Tissue Engineering and Regenerative Medicine Phuc Van Pham, 2019-08-14 This new series, based on a bi-annual conference and its topics, represents a major contribution to the emerging science of cancer research and regenerative medicine. Each volume brings together some of the most pre-eminent scientists working on cancer biology, cancer treatment, cancer diagnosis, cancer prevention and regenerative medicine to share information on currently ongoing work which will help shape future therapies. These volumes are invaluable resources not only for already active researchers or clinicians but also for those entering these fields, plus those in industry. Tissue Engineering and Regenerative Medicine is a proceedings volume which reflects papers presented at the 3rd bi-annual Innovations in Regenerative Medicine and Cancer Research conference; taken with its companion volume Stem Cells: Biology and Engineering it provides a complete overview of the papers from that meeting of international experts. |
| fxcycle pi/rnase staining solution: Mitochondrial Mechanisms of Degeneration and Repair in Parkinson's Disease Lori M. Buhlman, 2016-09-13 This volume brings together various theories of how aberrations in mitochondrial function and morphology contribute to neurodegeneration in idiopathic and familial forms of Parkinson’s disease. Moreover, it comprehensively reviews the current search for therapies, and proposes how molecules are involved in specific functions as attractive therapeutic targets. It is expected to facilitate critical thought and discussion about the fundamental aspects of neurodegeneration in Parkinson’s disease and foster the development of therapeutic strategies among researchers and graduate students. Theories of idiopathic Parkinson’s etiology support roles for chronic inflammation and exposure to heavy metals or pesticides. Interestingly, as this project proposes, a case can be made that abnormalities in mitochondrial morphology and function are at the core of each of these theories. In fact, the most common approach to the generation of animal and cell-culture models of idiopathic Parkinson’s disease involves exposure to mitochondrial toxins. Even more compelling is the fact that most familial patients harbor genetic mutations that cause disruptions in normal mitochondrial morphology and function. While there remains to be no effective treatment for Parkinson’s disease, efforts to postpone, prevent and “cure” onset mitochondrial aberrations and neurodegeneration associated with Parkinson’s disease in various models are encouraging. While only about ten percent of Parkinson’s patients inherit disease-causing mutations, discovering common mechanisms by which familial forms of Parkinson’s disease manifest will likely shed light on the pathophysiology of the more common idiopathic form and provide insight to the general process of neurodegeneration, thus revealing therapeutic targets that will become more and more accessible as technology improves. |
| fxcycle pi/rnase staining solution: Haploids in Crop Improvement II Constantine E. Don Palmer, Wilfred A. Keller, Kenneth J. Kasha, 2006-01-27 Doubled haploid technology is an important tool for plant breeding. It allows for significant time reduction in the achievement of homozygous breeding lines of value in crop improvement. This volume provides an excellent overview of haploid induction and the application of doubled haploids. The authors emphasize advances made in the understanding of microspore embryogenesis, but treat also advances in gynogenesis and the manipulation of parthenogenetic haploid development. The text contains a thorough discussion of the application of haploidy to the improvement of a number of species from various families, including Brassicaceae, Poaceae, and Solanaceae. The various methods applicable to these species are described in detail. Each chapter contains critical evaluation of the scientific literature and an extensive list of references. This volume is ideally suited for plant breeders, geneticists, and plant cell biologists. |
| fxcycle pi/rnase staining solution: Role of Nutraceuticals in Cancer Chemosensitization , 2017-10-18 Role of Nutraceuticals in Chemoresistance to Cancer, Volume Two, focuses on nutraceuticals, the compounds derived from natural sources, which are usually multi-targeted as a means to overcome chemoresistance. This book discusses the role of several compounds related to nutraceuticals and chemoresistance, such as curcumin, resveratrol, indole 3-carbinol, tocotrienols, ursolic acid, fisetin, celastrol, gambogic, butein, catechins and silymarin. It is a valuable resource for cancer researchers, oncologists and members of several areas of the biomedical field who are interested in understanding how to use nutraceuticals as a sensitizing agent for chemotherapy. - Brings updated information on natural compounds used as specific inhibitors of cell signaling pathways as reviewed by experts in the field - Presents experts analysis and summary of reported and novel findings and potential translational application in cancer patients - Describes molecular mechanisms with new and helpful approaches for the readers to use in their own investigations |
| fxcycle pi/rnase staining solution: Endothelium and Epithelium João Carrasco, Matheus Mota, 2012 This book presents topical research in the study of the composition, functions and pathology of endothelium and epithelium. Topics discussed include the pathophysiology of endothelium; the role of epithelial innate immunity in inflammatory diseases; morphofunctional characteristic features of statis endothelium and dynamic epithelial cell populations; changes of respiratory epithelium in bronchial asthma; interleukin-6 (IL6) signalling and its role in the development of cardiovascular disease; endothelium-dependent effects of heat shock on vasomotor tone; the Gasdermin Superfamily functioning in epithelial cells and tight junctions in upper airway epithelium. |
| fxcycle pi/rnase staining solution: Cytometry, Part A , 2000-10-31 Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area. - Contains 56 extensive chapters authored by world authorities on cytometry - Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases - Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine - Complements the first and second editions on flow cytometry in the Methods in Cell Biology series and includes new sections on technology principles |
| fxcycle pi/rnase staining solution: PI3K signalling Klaus Okkenhaug, Martin Turner, Michael R Gold, 2015-03-05 The PI3Ks control many key functions in immune cells. PI3Ks phosphorylate PtdIns(4,5)P2 to yield PtdIns(3,4,5)P3. Initially, PI3K inhibitors such as Wortmannin, LY294002 and Rapamycin were used to establish a central role for Pi3K pathway in immune cells. Considerable progress in understanding the role of this pathway in cells of the immune system has been made in recent years, starting with analysis of various PI3K and Pten knockout mice and subsequently mTOR and Foxo knockout mice. Together, these experiments have revealed how PI3Ks control B cell and T cell development, T helper cell differentiation, regulatory T cell development and function, B cell and T cell trafficking, immunoglobulin class switching and much, much more. The PI3Kd inhibitor idelalisib has recently been approved for the treatment of B cell lymphoma. Clinical trials of other PI3K inhibitors in autoimmune and inflammatory diseases are also in progress. This is an opportune time to consider a Research Topic considering when what we have learned about the PI3K signalling module in lymphocyte biology and how this is making an impact on clinical immunology and haematology. |
| fxcycle pi/rnase staining solution: Physics of Biological Membranes Patricia Bassereau, Pierre Sens, 2018-12-30 This book mainly focuses on key aspects of biomembranes that have emerged over the past 15 years. It covers static and dynamic descriptions, as well as modeling for membrane organization and shape at the local and global (at the cell level) scale. It also discusses several new developments in non-equilibrium aspects that have not yet been covered elsewhere. Biological membranes are the seat of interactions between cells and the rest of the world, and internally, they are at the core of complex dynamic reorganizations and chemical reactions. Despite the long tradition of membrane research in biophysics, the physics of cell membranes as well as of biomimetic or synthetic membranes is a rapidly developing field. Though successful books have already been published on this topic over the past decades, none include the most recent advances. Additionally, in this domain, the traditional distinction between biological and physical approaches tends to blur. This book gathers the most recent advances in this area, and will benefit biologists and physicists alike. |
| fxcycle pi/rnase staining solution: Dictionary of Food Compounds with CD-ROM Shmuel Yannai, 2003-10-24 The Dictionary of Food Compounds with CD-ROM: Additives, Flavors, and Ingredients provides comprehensive information on 30,000 compounds found in food, including: NATURAL FOOD CONSTITUENTS Lipids Proteins Carbohydrates Fatty acids Flavonoids Alkaloids FOOD ADDITIVES Colorants Preservatives Antioxidants Fl |
| fxcycle pi/rnase staining solution: Kinesins and Cancer Frank Kozielski, FSB, 2015-03-02 This interdisciplinary volume collates research work on kinesins and cancer. Authors attempt to validate members of the kinesin superfamily as potential targets for drug development in cancer chemotherapy. The work begins by highlighting the importance of kinesins, summarising current knowledge and how they are shown to be crucial for mitosis. Chapters go on to explore how this family of proteins are emerging as a novel target for chemotherapeutic intervention and drug development. Readers will learn how kinesins travel along microtubules to fulfill their many roles in intracellular transport or cell division. Several compounds that inhibit two mitotic kinesins (called Eg5 and CENP-E) have entered Phase I and II clinical trials and are explored in these chapters. Additional mitotic kinesins are currently being validated as drug targets, raising the possibility that the repertoire of kinesin-based drug targets may expand in the future. The book is suitable as a reference standard for the field of kinesins and cancer. It will interest those in academia and pharmaceutical companies, and anyone with an interest in the medical relevance of these proteins, which cutting edge methodologies are now enabling us to understand in astonishing detail. |
| fxcycle pi/rnase staining solution: Advances in Cancer Research , 2018-01-24 Advances in Cancer Research, Volume 137, the latest release in this ongoing, well-regarded serial provides invaluable information on the exciting and fast-moving field of cancer research. This volume presents original reviews on research bridging oncology and gene expression, with this volume covering unconventional approaches to modulating the immunogenicity of tumor cells, tumor dormancy and immunoediting, the emerging role of anti-apoptotic Bcl-2 family proteins in chemoresistance, Beclin-1 and autophagy, MDA-7/IL-24, and nanotechnology and medicine. |
| fxcycle pi/rnase staining solution: Boron Nitride Nanotubes in Nanomedicine Gianni Ciofani, Virgilio MATTOLI, 2016-04-26 Boron Nitride Nanotubes in Nanomedicine compiles, for the first time in a single volume, all the information needed by researchers interested in this promising type of smart nanoparticles and their applications in biomedicine. Boron nitride nanotubes (BNNTs) represent an innovative and extremely intriguing class of nanomaterials. After introducing BNNTs and explaining their preparation and evaluation, the book shows how the physical, chemical, piezoelectric and biocompatibility properties of these nanotubes give rise to their potential uses in biomedicine. Evidence is offered (from both in vitro and in vivo investigations) for how BNNTs can be useful in biomedical and nanomedicine applications such as therapeutic applications, tissue regeneration, nanovectors for drug delivery, and intracellular nanotransducers. - Covers a range of promising biomedical BNNT applications - Provides great value not just to academics but also industry researchers in fields such as materials science, molecular biology, pharmacology, biomedical engineering, and biophysical sciences - Offers evidence for how BNNTs can be useful in biomedical and nanomedicine applications such as therapy, tissue regeneration, nanovectors for drug delivery, and intracellular nanotransducers - Incorporates, for the first time in a single volume, all the information needed by researchers interested in this promising type of smart nanoparticles and their applications in biomedicine |
| fxcycle pi/rnase staining solution: Nanomaterial David G. Capco, Yongsheng Chen, 2014-03-28 The rapidly developing field of nanomaterials has expanded in many commercial areas. More recent studies have begun to provide a foundation for understanding how nanomaterials influence cells and how they also can serve as methodological tools for studies in medicine and cell biology, including research into stem cells. Recent investigations have shown affects of nanomaterials on specific subcellular structures, such as the actin-based brush border network in cells with an increasing emphasis on the barrier function of epithelial tissues. While other studies have shown involvement of nanoparticles in specific cytoplasmic signal transduction events such as the rise in intracellular free calcium, a signaling event known to regulate many changes in cell architecture and function. In parallel, nanomaterials are increasingly used in medicine for drug delivery, treatment of cancer and an increasing number of new applications. This book investigates these areas and also includes new methods for assessment in cell biology and medicine. |
| fxcycle pi/rnase staining solution: Cardiac Development Margaret L. Kirby, 2007-03-22 This is the only in-depth, single author survey of heart development. It will provide a more systematic, up-to-date synthesis of the subject than any other volume, spanning the range from classical anatomical studies to recent findings in molecular biology. It also covers topics that are often omitted from discussions of heart development, such as myocardial function, cardiac innervation, and conduction development and clinical correlates will be discussed throughout. The book is beautifully illustrated by Karen Waldo, an artist who has collaborated with Dr. Kirby for many years. |
| fxcycle pi/rnase staining solution: Multi-Drug Resistance in Cancer Jun Zhou, 2012-08-09 Chemotherapy is one of the major treatment options for cancer patients; however, the efficacy of chemotherapeutic management of cancer is severely limited by multidrug resistance, in that cancer cells become simultaneously resistant to many structurally and mechanistically unrelated drugs. In the past three decades, a number of mechanisms by which cancer cells acquire multidrug resistance have been discovered. In addition, the development of agents or strategies to overcome resistance has been the subject of intense study. This book contains comprehensive and up-to-date reviews of multidrug resistance mechanisms, from over-expression of ATP-binding cassette drug transporters such as P-glycoprotein, multidrug resistance-associated proteins, and breast cancer resistance p- tein to the drug ratio-dependent antagonism and the paradigm of cancer stem cells. The book also includes strategies to overcome multidrug resistance, from the development of compounds that inhibit drug transporter function to the modulation of transporter expression. In addition, this book contains techniques for the detection and imaging of drug transporters, methods for the investigation of drug resistance in animal models, and strategies to evaluate the efficacy of resistance reversal agents. The book intends to provide a state-of-the-art collection of reviews and methods for both basic and clinician investigators who are interested in cancer multidrug resistance mechanisms and reversal strategies. Tianjin, China Jun Zhou v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Multidrug Resistance in Cancer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Bruce C. Baguley 2 Multidrug Resistance in Oncology and Beyond: From Imaging of Drug Efflux Pumps to Cellular Drug Targets . . . . . . . . . . . . . . . . . . . . . . . . . . |
| fxcycle pi/rnase staining solution: Microbial Toxins P. Gopalakrishnakone, 2018-03-14 In recent years, the field of Toxinology has expanded substantially. On the one hand it studies venomous animals, plants and micro organisms in detail to understand their mode of action on targets. While on the other, it explores the biochemical composition, genomics and proteomics of toxins and venoms to understand their three interaction with life forms (especially humans), development of antidotes and exploring their pharmacological potential. Therefore, Toxinology has deep linkages with biochemistry, molecular biology, anatomy and pharmacology. In addition, there is a fast developing applied subfield, clinical toxinology, which deals with understanding and managing medical effects of toxins on human body. Given the huge impact of toxin-based deaths globally, and the potential of venom in generation of drugs for so-far incurable diseases (for example, Diabetes, Chronic Pain), the continued research and growth of the field is imminent. This has led to the growth of research in the area and the consequent scholarly output by way of publications in journals and books. Despite this ever growing body of literature within biomedical sciences, there is still no all-inclusive reference work available that collects all of the important biochemical, biomedical and clinical insights relating to Toxinology. The Handbook of Toxinology aims to address this gap and cover the field of Toxinology comprehensively. |
| fxcycle pi/rnase staining solution: Handbook of Flow Cytometry Methods J. Paul Robinson, 1993-03-25 Handbook of Flow Cytometry Methods Edited By J. Paul Robinson Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory manual for investigators involved in research and diagnostic applications of flow cytometry. Handbook of flow cytometry methods offers a collection of proven, basic procedures designed for use in the flow cytometry laboratory. Methods implemented at Purdue University Cytometry Laboratories and developed in laboratories of contributors worldwide provide practical, step-by-step procedures for: the preparation and handling of standards,reagents and specimens laboratory analyses for immunophenotyping measurement of DNA content and cell cycle analyses determination of cell physiology and function set up and operation of flow cytometers computational and data analysis techniques safety precautions Drawing on their collective experience at leading research centers, the editors have fashioned a unique working manual intended to serve as the starting point for the evaluation and establishment of laboratory benchmark standards in flow and image cytometry. Each method is described in detail to be used and modified depending on the user’s needs. Special features of Handbook of Flow Cytometry Methods include: ample space throughout for personal annotations suggestions of alternative procedures; trouble-shooting tips extensive list of reagents and suppliers comb-bound format for ease of use at the lab bench Handbook of Flow Cytometry Methods is an essential laboratory resource for students, faculty, and researchers in cell and molecular biology, immunology, cancer biology, hematology/oncology, and laboratory medicine. |
| fxcycle pi/rnase staining solution: LC-MS/MS in Proteomics Pedro R. Cutillas, John F. Timms, 2011-04-06 With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem. |
| fxcycle pi/rnase staining solution: Hormone Related Tumors Hiroshi Nagasawa, Kaoru Abe, 1981 |
| fxcycle pi/rnase staining solution: Active Polymers: Volume 1190 Andreas Lendlein, V. Prasad Shastri, K. Gall, 2009-11-19 The MRS Symposium Proceeding series is an internationally recognised reference suitable for researchers and practitioners. |
| fxcycle pi/rnase staining solution: Chlordane Hazards to Fish, Wildlife, and Invertebrates Ronald Eisler, 1990 |
| fxcycle pi/rnase staining solution: Inflammation and Gastrointestinal Cancers Janusz A. Z. Jankowski, 2011-08-08 Inflammation in gastrointestinal mucosa can remodel the topography of the overlying epithelium. If such inflammation is chronic, it has fundamental clinical consequences, the principal of which is premalignant metaplasia throughout the alimentary tract. Furthermore, mucosal inflammation, even if subtle, is the single most common pathway for GI cancer. This book discusses all aspects of the relation between inflammation and GI cancer, from the basic science through to the translational science which is helping in the optimization of clinical management strategies. Among the topics considered are the impact of inherited syndromes; the roles of acid reflux, H. pylori, inflammatory bowel disease, and primary sclerosing cholangitis; screening strategies; targeted drug therapies; genetics; and the use of endoscopic methods. The authors are the best in their field, and this book is designed for the enthusiastic student as well as the professional in GI science and medicine. |
| fxcycle pi/rnase staining solution: Photochemistry and Photophysics of Coordination Compounds I Vincenzo Balzani, Sebastiano Campagna, 2007-07-31 This book presents critical reviews of the current position and future trends in modern chemical research. It offers short and concise reports on chemistry, each written by world renowned experts. |
| fxcycle pi/rnase staining solution: Apoptosis in Cancer Pathogenesis and Anti-cancer Therapy Christopher D. Gregory, 2016-08-24 This book discusses properties of apoptosis and other cell death modalities in cancer pathogenesis and treatment. Its nine chapters discuss modulation of anti-tumor inflammatory and immune responses, effects on the tumor microenvironment, to strategies for improving pro-apoptotic therapies, mechanisms and implications for disease pathogenesis, axl and mer receptor tyrosine kinases, immunogenic apoptotic cell death and anti-cancer immunity and cancer cell death-inducing radiotherapy. This book places the onco-biology of apoptosis in clear and objective perspective through an expertly synthesized series of reviews. Apoptosis in Cancer Pathogenesis and Anti-cancer Therapy is a deft and thorough exploration of cutting-edge research in apoptosis and anti-cancer mechanisms from basic biology to oncology. It highlights a rapidly growing field within cancer research and is essential reading for oncologists, biochemists and advanced graduate students alike. |
| fxcycle pi/rnase staining solution: Carbohydrate Metabolism and Its Disorders , 1968 |
| fxcycle pi/rnase staining solution: The Integrin Interactome Miguel Vicente-Manzanares, 2020-11-20 This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology. |
| fxcycle pi/rnase staining solution: Airways and Vascular Remodelling in Asthma and Cardiovascular Disease Clive Page, Judith Black, 1994-11-10 Vascular diseases and asthma are two common diseases associated with considerable morbidity and mortality. Both are chronic, each has an inflammatory component, both are accompanied by remodelling of hollow tubes and both are amenable to early intervention. This book compares and contrasts research progress made in each area, including our current understanding of the pathophysiology involved and the results of large-scale multicentre trials. |
| fxcycle pi/rnase staining solution: Flow Cytometry Zbigniew Darzynkiewicz, J. Paul Robinson, 1994 Flow Cytometry, Second Edition is a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Unique comprehensive methodological coverage * In-depth treatment of procedures, including: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who developed or modified the techniques * Methods cover cell death and cell cycle analyses * Practical, handbook-style presentation works in lab and in the classroom * Color plates illustrate technique |
| fxcycle pi/rnase staining solution: Autophagosome and Phagosome Vojo Deretic, 2008-04-24 Autophagy and phagocytosis are distinct yet partially morphologically similar processes. Understanding them is vital for the studies of cancer, aging, neurodegeneration, immunology, and infectious diseases. This book presents autophagosome and phagosome methods for novices and advanced researchers alike. Comprehensive and forward-thinking, the book offers a valuable guide to both cellular processes while inciting researchers to explore their potentially important connections. |
| fxcycle pi/rnase staining solution: Developmental Cardiology Edward B. Clark, Atsuyoshi Takao, 1990-01-01 |
| fxcycle pi/rnase staining solution: The Molecular Probes Handbook Iain Johnson, 2010 The most complete fluorescent labeling and detection reference available, The Molecular Probes HandbookA Guide to Fluorescent Probes and Labeling Technologies contains over 3,000 technology solutions representing a wide range of biomolecular labeling and detection reagents. The significantly revised 11th Edition features extensive references, reorganized content, and new technical notes and product highlights. |
| fxcycle pi/rnase staining solution: High Resolution Electron Microscopy of Defects in Materials: Volume 183 Materials Research Society, 1990-08-10 The MRS Symposium Proceeding series is an internationally recognised reference suitable for researchers and practitioners. |
| fxcycle pi/rnase staining solution: Practical Flow Cytometry Howard M. Shapiro, 2005-02-25 From the reviews of the 3rd Edition... The standard reference for anyone interested in understandingflow cytometry technology. American Journal of Clinical Oncology ...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating. Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers. |
| fxcycle pi/rnase staining solution: The Hematopoietic Microenvironment Michael W. Long, Max S. Wicha, 1993 |
| fxcycle pi/rnase staining solution: Cancer Cell Metabolism , 2020 This book illustrates various aspects of cancer cell metabolism, including metabolic regulation in solid tumours vs. non-solid tumours, the molecular pathways involved in its metabolism, and the role of the tumour microenvironment in the regulation of cancer cell metabolism. It summarizes the complexity of cancer cell metabolism in terms of the switch from anaerobic to aerobic glycolysis and how mitochondrial damage promotes aerobic glycolysis in cancer cells. The respective chapters provide the latest information on the metabolic remodelling of cancer cells and elucidate the important role of the signalling pathways in reprogramming of cancer cell metabolism. In addition, the book highlights the role of autophagy in cancer cell metabolism, and how metabolic crosstalk between cancer cells and cancer-associated fibroblasts promotes cancer cell progression. In closing, it summarizes recent advancements in drug development through targeting cancer metabolism. |
FxCycleTM PI/RNase Staining Solution - Thermo Fisher Scientific
Mar 9, 2015 · Univariate DNA content analysis is an established assay method and is widely used for studies in oncology, cell biology, and molecular biology. The FxCycleTM PI/RNase Staining …
PI/RNase Staining Buffer - BD Biosciences
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases.
Store at 4°C Propidium Iodide (PI)/RNase Staining Solution
Propidium Iodide (PI)/RNase 4°C Revision 4 Background: Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is …
FxCycle™ Far Red Stain - Thermo Fisher Scientific
FxCycleTM Far Red stains RNA as well as dsDNA, so addition of RNase A is required for DNA content analysis. For long-term storage, the dye is supplied as five vials of solid dye and stored …
PI/RNASE Solution - immunostep.com
Propidium Iodide (PI) is the most commonly used dye for DNA and cell cycle analysis for flow cytometry. The PI binds to DNA by intercalating into the double stranded macromolecule.
PI Cell Cycle Analysis Kit (with RNase) - 4abio.com
degraded by RNase, the nucleic acid dye in this kit bind with DNA composed of chromatin in the nucleus. And the results can be analyzed by flow cytometry. REAGENTS PI Cell Cycle Analysis Kit …
PI/RNase Staining Solution - sungenebiotech.com
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to Product Information both DNA and RNA, so the latter must be removed by digestion with ribonucleases.
Propidium Iodide Staining for DNA Content - UMass Chan …
Adjust cell concentration to 2x106 cells/100 l in PBS. Add 900 l of 95% ethanol, dropwise, to the cells while vortexing gently. Store cells at 4°C to fix. Fix cells overnight, or ideally for 24 hours. If …
PMS 425 C QuICk ReFeReNCe TITLe QuICk ReFeReNCe: …
FxCycle™ PI/RNAse Staining Solution 1. Begin with 1 × 106 cells that have been ethanol fixed. Wash cells once and remove the buffer from the cell pellet. 2. ™Add 0.5 mL of FxCycle PI/RNAse …
PI/RNase Staining Solution - mesgenbio.com
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The content of DNA as …
Propidium Iodide Cell cycle staining for cytoplasmic GFP …
For optimal PI staining, cells require permeabilization with ethanol to allow the dye access to the nucleus. However, such treatment with ethanol results in the loss of GFP fluorescence as the …
CellCycle™ PI/RNAse Staining Solution - ABP Bio
The CellCycle™ PI/RNAse staining solution is a specially formulated ready-to-use reagent. Just adding it to fixed cells, incubate, and acquire on a flow cytometer without washing.
Immunofluorescence staining of cells in combination with PI …
Immunofluorescence staining of cells in combination with PI staining of cells for cell cycle analysis Protocol This method provides a general procedure for DNA staining for cell cycle analysis using …
Life Technologies CERTIFICATE OF ANALYSIS
Life Technologies Corporation certifies on the date above that this is an accurate record of the analysis of the subject lot, and that the data conform to the specifications in effect for this …
Propidium Iodide Nucleic Acid Stain - Thermo Fisher Scientific
To make a stock solution from the solid form, dissolve PI (MW = 668.4) in deionized water (dH2O) at 1 mg/mL (1.5 mM) and store at 2–6°C, protected from light. When stored properly, solutions …
Isothiocyanate-induced Cell Cycle Arrest in a Novel In Vitro …
Polyvinylidene Difluoride (PVDF) membranes, page ruler plus protein marker, ECL and FxCycle PI/RNase staining solution were supplied by Thermo Fisher Scientific (Waltham, MA, USA).
Fluorophore Selection Guide
Register for our monthly flow cytometry educational webinar series to help optimize your workflow. Visit thermofisher.com/letstalkflow. For Research Use Only. Not for use in diagnostic procedures. …
Supplementary materials and methods
Cell cycle populations were determined by PI-A. Antiviral CPE assays of EV71, HSV-1, Influenza A Virus, VV and HIV-1 All these assays were performed in Wuxi Apptec.
Using the Attune NxT Flow Cytometer for rapid and accurate …
Flow cytometric analysis of plant nuclei prepared from A. thaliana Col-1 leaf tissue and labeled with FxCycle PI/RNase Staining Solution. Data were collected with the 532 nm laser.
Fluorophore selection guide
InvitrogenTM AttuneTM XenithTM Flow Cytometer. Register for our monthly flow cytometry educational webinar series to help optimize your workflow. Visit thermofisher.com/letstalkflow. …
FxCycleTM PI/RNase Staining Solution - Thermo Fisher …
Mar 9, 2015 · Univariate DNA content analysis is an established assay method and is widely used for studies in oncology, cell biology, and molecular biology. The FxCycleTM PI/RNase Staining …
PI/RNase Staining Buffer - BD Biosciences
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases.
Store at 4°C Propidium Iodide (PI)/RNase Staining Solution
Propidium Iodide (PI)/RNase 4°C Revision 4 Background: Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is …
FxCycle™ Far Red Stain - Thermo Fisher Scientific
FxCycleTM Far Red stains RNA as well as dsDNA, so addition of RNase A is required for DNA content analysis. For long-term storage, the dye is supplied as five vials of solid dye and …
PI/RNASE Solution - immunostep.com
Propidium Iodide (PI) is the most commonly used dye for DNA and cell cycle analysis for flow cytometry. The PI binds to DNA by intercalating into the double stranded macromolecule.
PI Cell Cycle Analysis Kit (with RNase) - 4abio.com
degraded by RNase, the nucleic acid dye in this kit bind with DNA composed of chromatin in the nucleus. And the results can be analyzed by flow cytometry. REAGENTS PI Cell Cycle …
PI/RNase Staining Solution - sungenebiotech.com
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to Product Information both DNA and RNA, so the latter must be removed by digestion with ribonucleases.
Propidium Iodide Staining for DNA Content - UMass Chan …
Adjust cell concentration to 2x106 cells/100 l in PBS. Add 900 l of 95% ethanol, dropwise, to the cells while vortexing gently. Store cells at 4°C to fix. Fix cells overnight, or ideally for 24 hours. …
PMS 425 C QuICk ReFeReNCe TITLe QuICk ReFeReNCe: …
FxCycle™ PI/RNAse Staining Solution 1. Begin with 1 × 106 cells that have been ethanol fixed. Wash cells once and remove the buffer from the cell pellet. 2. ™Add 0.5 mL of FxCycle …
PI/RNase Staining Solution - mesgenbio.com
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The content of DNA …
Propidium Iodide Cell cycle staining for cytoplasmic GFP …
For optimal PI staining, cells require permeabilization with ethanol to allow the dye access to the nucleus. However, such treatment with ethanol results in the loss of GFP fluorescence as the …
CellCycle™ PI/RNAse Staining Solution - ABP Bio
The CellCycle™ PI/RNAse staining solution is a specially formulated ready-to-use reagent. Just adding it to fixed cells, incubate, and acquire on a flow cytometer without washing.
Immunofluorescence staining of cells in combination with PI …
Immunofluorescence staining of cells in combination with PI staining of cells for cell cycle analysis Protocol This method provides a general procedure for DNA staining for cell cycle analysis …
Life Technologies CERTIFICATE OF ANALYSIS
Life Technologies Corporation certifies on the date above that this is an accurate record of the analysis of the subject lot, and that the data conform to the specifications in effect for this …
Propidium Iodide Nucleic Acid Stain - Thermo Fisher Scientific
To make a stock solution from the solid form, dissolve PI (MW = 668.4) in deionized water (dH2O) at 1 mg/mL (1.5 mM) and store at 2–6°C, protected from light. When stored properly, solutions …
Isothiocyanate-induced Cell Cycle Arrest in a Novel In Vitro …
Polyvinylidene Difluoride (PVDF) membranes, page ruler plus protein marker, ECL and FxCycle PI/RNase staining solution were supplied by Thermo Fisher Scientific (Waltham, MA, USA).
Fluorophore Selection Guide
Register for our monthly flow cytometry educational webinar series to help optimize your workflow. Visit thermofisher.com/letstalkflow. For Research Use Only. Not for use in diagnostic …
Supplementary materials and methods
Cell cycle populations were determined by PI-A. Antiviral CPE assays of EV71, HSV-1, Influenza A Virus, VV and HIV-1 All these assays were performed in Wuxi Apptec.
Using the Attune NxT Flow Cytometer for rapid and …
Flow cytometric analysis of plant nuclei prepared from A. thaliana Col-1 leaf tissue and labeled with FxCycle PI/RNase Staining Solution. Data were collected with the 532 nm laser.
Fluorophore selection guide
InvitrogenTM AttuneTM XenithTM Flow Cytometer. Register for our monthly flow cytometry educational webinar series to help optimize your workflow. Visit thermofisher.com/letstalkflow. …
