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| epitope mapping peanut allergy: Finding a Path to Safety in Food Allergy National Academies of Sciences, Engineering, and Medicine, Health and Medicine Division, Food and Nutrition Board, Committee on Food Allergies: Global Burden, Causes, Treatment, Prevention, and Public Policy, 2017-05-27 Over the past 20 years, public concerns have grown in response to the apparent rising prevalence of food allergy and related atopic conditions, such as eczema. Although evidence on the true prevalence of food allergy is complicated by insufficient or inconsistent data and studies with variable methodologies, many health care experts who care for patients agree that a real increase in food allergy has occurred and that it is unlikely to be due simply to an increase in awareness and better tools for diagnosis. Many stakeholders are concerned about these increases, including the general public, policy makers, regulatory agencies, the food industry, scientists, clinicians, and especially families of children and young people suffering from food allergy. At the present time, however, despite a mounting body of data on the prevalence, health consequences, and associated costs of food allergy, this chronic disease has not garnered the level of societal attention that it warrants. Moreover, for patients and families at risk, recommendations and guidelines have not been clear about preventing exposure or the onset of reactions or for managing this disease. Finding a Path to Safety in Food Allergy examines critical issues related to food allergy, including the prevalence and severity of food allergy and its impact on affected individuals, families, and communities; and current understanding of food allergy as a disease, and in diagnostics, treatments, prevention, and public policy. This report seeks to: clarify the nature of the disease, its causes, and its current management; highlight gaps in knowledge; encourage the implementation of management tools at many levels and among many stakeholders; and delineate a roadmap to safety for those who have, or are at risk of developing, food allergy, as well as for others in society who are responsible for public health. |
| epitope mapping peanut allergy: Molecular Allergy Diagnostics Jörg Kleine-Tebbe, Thilo Jakob, 2017-05-08 This book, based on a recent German publication, offers an overview of basic data and recent developments in the groundbreaking field of molecular allergology. It comprehensively explores the origin and structure of single allergen molecules (components) and their utility in improving the management of type I, IgE-mediated allergic reactions and disorders like allergic respiratory diseases, food allergies, and anaphylaxis. Highly specific testing, called component-resolved diagnostics, aims to identify and utilize single molecules. Over 200 single allergens from plant or animal sources have been applied to single or multiplex laboratory testing for the presence of allergen-specific IgE. This leap in assay sensitivity and specificity has led to three major advances in patient management: discrimination between primary allergic sensitization and complex cross-reactivity, recognition of IgE profiles for certain allergens and identification of patients most likely to benefit from allergen-specific immunotherapy. The book discusses in detail the benefits and limitations of this 21st century technology, and offers suggestions for the use of molecular allergology in routine clinical practice. It is a “must read” for physicians treating allergic patients as well as scientists interested in natural allergic molecules and their interactions with the human immune system. |
| epitope mapping peanut allergy: Encyclopedia of Food Allergy , 2024-06-21 Encyclopedia of Food Allergy, organized in 10 sections, with ~200 chapters, and written by world-renowned clinician-scientist authors, is the most comprehensive resource for food allergy ever compiled. With online and physical presence, intuitive and easily accessible organization of information, the reader can quickly access overview and general topics as well as detailed information to inform solutions to clinical or research questions. Research topics provide the necessary background for the novice as well as the details required for those in the field. Clinical topics provide comprehensive and practical information, with generous use of tables, figures, and key points/clinical pearls, to inform clinical decision-making, and promote evidence-based management decisions. Food allergy may affect up to 10% of the population in developed countries and appears to be increasing in prevalence worldwide, with many food allergies proving life-long, severe and potentially fatal. The last decade has witnessed a sea change response to the impact of food allergy through basic science research on the immunology, food science research on the triggers, clinical approaches to daily management, treatment and prevention, and an increasing understanding of the psychosocial and societal implications and how to address them. With the expanding breadth and depth of the field, there is no existing comprehensive resource available for those professionals interested in learning about or contributing to food allergy research and clinical care. This is a complete resource covering broad and detailed aspects of food allergy and adverse food reactions for clinicians, researchers, regulators, food industry, students and other stakeholders who need and will benefit from a rich resource with in-depth and practical information. - Presents in-depth, comprehensive coverage from an outstanding international author base of domain experts - Ideal for new researchers and clinicians who will have a single resource that includes general topics to get them started - Includes access to detailed information in their areas of work AND for many related topics that will help improve their research or clinical care |
| epitope mapping peanut allergy: Protein Microarrays for Disease Analysis Rodrigo Barderas, Joshua LaBaer, Sanjeeva Srivastava, 2021-07-11 This volume focuses on protein analysis, and covers a wide array of uses of protein microarray for disease analysis. The chapters in this book discuss different stages of protein microarrays from their construction to their use, including different types of protein microarrays such as recombinant proteins, antibody, phage, and NAPPA protein microarrays, in planar format or in solution via beads arrays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Protein Microarrays for Disease Analysis: Methods and Protocols is a valuable resource for graduate and post-doctoral fellows interested in protein microarrays, as well as senior researchers interested in gaining more insight into this developing field. |
| epitope mapping peanut allergy: Plant Food Allergens E. N. Clare Mills, Peter R. Shewry, 2008-04-15 Plant Food Allergens is concerned with a paradox of immense, potentially life-threatening significance to about 1 in 100 adults and 1 in 10 children. The paradox is that certain nutritious proteins from wholesome foods can act as if the were harmful, sometimes deadly poisons, to these people who possess an allergy to them. In order to study the complex problems of food allergy a EU funded network, called PROTALL was set up, bringing together a wide range of clinicians and scientists. This important book is largely based on the outcome of its investigations. Written by over 30 acknowledged experts and carefully edited by Dr Clare Mills and Professor Peter Shewry, themselves well known internationally; this important work covers all major aspects of the subject. Commencing with introductory chapters, the comprehensive contents of Plant Food Allergens includes details of the major allergens including: plant lipid transfer proteins, the 2S albumin proteins, the cereal á-amylase/trypsin family, latex and plant chitinases, profilins, bet v 1-homologous allergens and plant seed globulins. The book concludes with important chapters on the assessment of the allergenicity of novel and GM foods, and the monitoring of and technological effects on allergenicity of proteins in the food industry. Plant Food Allergens is an essential purchase for a wide range of scientists and clinicians including plant and agricultural scientists, chemists, allergy specialists, food scientists and technologists, pharmacologists, physiologists and nutritionists. Libraries in all research establishments and universities researching and teaching these subjects will need copies of this important book on their shelves Dr Clare Mills is based at The Institute of Food Research, Norwich, UK. Professor Peter Shewry is based at Rothamsted Research, Harpenden, UK. |
| epitope mapping peanut allergy: Evolution of Translational Omics Institute of Medicine, Board on Health Sciences Policy, Board on Health Care Services, Committee on the Review of Omics-Based Tests for Predicting Patient Outcomes in Clinical Trials, 2012-09-13 Technologies collectively called omics enable simultaneous measurement of an enormous number of biomolecules; for example, genomics investigates thousands of DNA sequences, and proteomics examines large numbers of proteins. Scientists are using these technologies to develop innovative tests to detect disease and to predict a patient's likelihood of responding to specific drugs. Following a recent case involving premature use of omics-based tests in cancer clinical trials at Duke University, the NCI requested that the IOM establish a committee to recommend ways to strengthen omics-based test development and evaluation. This report identifies best practices to enhance development, evaluation, and translation of omics-based tests while simultaneously reinforcing steps to ensure that these tests are appropriately assessed for scientific validity before they are used to guide patient treatment in clinical trials. |
| epitope mapping peanut allergy: Food Allergy Andreas L. Lopata, 2017-09-07 Food allergy is an adverse immunological reaction to allergens present in food. Up to 4% adults and 8% children are affected by food allergy. The increase in allergic diseases to food has led to the need for better diagnostics and more effective therapeutic approaches. This book describes the molecular biology and immunology of major food allergens, from laboratory based science to clinical immunology, encompassing novel characterisation and quantification methods, the application of recombinant food allergens in molecular diagnosis and the development of novel therapeutics. This book is the ideal reference tool for researchers, students and allergy clinicians to accurately diagnose and manage food allergies. |
| epitope mapping peanut allergy: Food Allergy Dean D. Metcalfe, Hugh A. Sampson, Ronald A. Simon, 2011-08-31 Applying a scientific approach this unique book covers both pediatric and adult adverse reactions to foods and food additives. Following the successful formula of the previous editions, Food Allergy has established itself asthe comprehensive reference for those treating patients with food allergy or suspected allergy. The book has been thoroughly revised and updated presenting new chapters devoted to food biotechnology and genetic engineering, seafood toxins, future approaches to therapy and hidden food allergens. Food Allergy, fourth edition, is divided into five sections featuring key concept boxes for each chapter. Displayed in a logical manner the book is a practical, readable reference for use in the hospital or private practice setting. |
| epitope mapping peanut allergy: Chemical and Biological Properties of Food Allergens Lucjan Jedrychowski, Harry J. Wichers, 2009-09-28 In the U.S. alone, severe food-related allergic reactions account for an estimated 30,000 emergency room visits and 150 deaths per year - unsettling statistics for food product developers and manufacturers who are charged with ensuring food safety and quality throughout the entire farm-to-table production chain. Providing the clear-cut information |
| epitope mapping peanut allergy: Multidisciplinary Approaches to Allergies Zhong-Shan Gao, Hua-Hao Shen, Min Zheng, Lynn J. Frewer, Luud J. W. J. Gilissen, 2013-07-27 Allergy is an immunological disease caused by multiple factors and characterized by variability, specificity and complexity. Multidisciplinary Approaches to Allergies covers diverse aspects ranging from basic molecular mechanisms to societal issues within the framework of multidisciplinary approaches to allergies. It contains 29 chapters in 6 parts: General Allergy; Allergenic Sources and Allergens; Diagnosis; Therapies and Pharmacy; Hypoallergenic Products; Environment, Hygiene and Societal Issues. It can be used in education and research as introductory and supplementary material. It is also an indispensable tool for scientists and doctors who are searching for an integrated way for allergy prevention, treatment and management. Zhong-Shan Gao, Ph.D., is Professor of Fruit Science and Allergy at the Department of Horticulture and the Allergy Research Center, Zhejiang University, China. Hua-Hao Shen, M.D., Ph.D., is Professor of Respiratory Diseases at the Second Affiliated Hospital, School of Medicine, Zhejiang University, China, and Professor in the Chang Jiang Scholars Program (MOE). Min Zheng, M.D., Ph.D., is Professor of Dermatology at the Second Affiliated Hospital, School of Medicine, Zhejiang University, China. Lynn J. Frewer, Ph.D., is Professor of Food & Society at the School of Agriculture, Food and Rural Development, Newcastle University, UK. Luud J.W.J. Gilissen, Ph.D., is senior researcher at Plant Research International, Wageningen University and Research Centre, the Netherlands. |
| epitope mapping peanut allergy: Food Allergy, An Issue of Immunology and Allergy Clinics Anna H. Nowak-Wegrzyn, 2012-02-28 This issue of Immunology and Allergy Clinics provides the latest essential updates in food allergies. Topics include the following: mechanisms of allergic sensitization to foods—bypassing immune tolerance pathways; determinants of food allergenicity; epidemiology of food allergy; prevention of food allergy through manipulating the timing of food exposure; diagnosis and management of eosinophilic esophagitis; the burden of food allergies and mental health issues; state-of-the-art and new horizons in food allergy diagnostic testing; food-induced anaphylaxis; oral tolerance; immunotherapy for food allergy; complementary and alternative medicine for food allergy; paradigm shift in management of milk and egg allergy—baked milk and egg diet. |
| epitope mapping peanut allergy: Food Allergy Linglin Fu, Bobby J. Cherayil, Haining Shi, Yanbo Wang, Yang Zhu, 2019-04-03 This book addresses the molecular mechanisms of food allergies and related control strategies. To do so, it covers a broad range of topics, including: the basic immunology of food allergies, including crosstalk between gut mucosal immunity and allergens; types of food allergens, structure of food allergen epitopes and cross-reactivity; detection and quantification methods for food allergens; in vitro and in vivo models for evaluating allergenicity; novel food processing methods for the development of hypoallergenic foods; bioactive natural compounds and functional foods for alleviating allergic reactions; modulation of the microbiota in food allergies and use of probiotics in allergic response regulation; and risk assessment and control strategies for food allergens. The information provided will enable food scientists/specialists to design safer and more functional food products, and will help regulatory agencies identify and label food allergens (and thus help consumers avoid allergic reactions). It will help clinicians and public health investigators prevent or treat outbreaks of food allergies, and will provide food producers and processors, as well as government inspectors, with valuable insights into evaluation, risk assessment and control strategies for allergens. Lastly, it will benefit upper-level undergraduate and graduate students in food science and safety, public health, medicine, nutrition and related fields. |
| epitope mapping peanut allergy: New Approach Methods in Immunology Jeffrey John Bajramovic, Susan Gibbs, Emanuela Corsini, Thomas Hartung, 2024-09-27 Currently, the assessment of functional immunological relevance is mainly done in animal models. Motivation to work on non-animal methods, or new approach methods (NAM), stems from economical and ethical considerations, and is supported by public pressure. Importantly, the translational gap between results obtained in animal studies and clinical trials in humans (the ‘valley of death’), combined with the reproducibility crisis in science, also provide strong scientific arguments to work on novel, robust, human-based methodology. The field of immunology confronts NAM scientists with specific challenges. Firstly, immunological responses require several cell types in different locations for proper development and take considerable time to develop. Secondly, immunological responses in outbred humans are characterized by genetic and functional variability. Still, the development and application of NAM are increasing rapidly, and the field is moving at such a fast pace that a special issue is timely. Our goal is to provide an overview of the current state-of-the-art regarding new approach methods or non-animal methods (NAM) in immunology. These should be inspired by the desire to mimic in vivo biology and describe e.g. challenges in mimicking immunological structures (like lymph nodes, bone marrow, local immune structures), immunological responses (systemic and local, innate and adaptive, B cells and T cells) and/or immunological processes (like maturation, trafficking, extravasation, immunotoxicity, affinity maturation). |
| epitope mapping peanut allergy: Pediatric Food Allergy Ruchi S. Gupta, 2020-03-02 Comprehensive and practical, this book thoroughly addresses the full range of concerns related to food allergies in the pediatric patient. As food allergies in the pediatric population increase in number and severity, Pediatric Food Allergy: A Clinical Guide provides information on new guidelines and potential treatment options, as well as working to improve awareness, diagnosis, management and prevention practices. Written by experts in their respective fields, chapters are divided into five sections. Opening with an introduction and overview of particular concerns and issues specific to food allergy in the pediatric population, sections two and three address diagnosis and management of comorbid conditions in food allergy, along with development of food allergies and current prevention recommendations. Sections four and five cover food allergy management, prognosis, and therapeutic options with a look to future developments, while all sections include a discussion of epidemiology, differential diagnoses of other potential food-related diseases. In Pediatric Food Allergy: A Clinical Guide, pediatricians and allergists alike will find an invaluable resource as they work with this vulnerable patient population. |
| epitope mapping peanut allergy: Allergy and Allergic Diseases A. Barry Kay, Jean Bousquet, Patrick G. Holt, Allen P. Kaplan, 2009-01-26 Reserve your copy now This two volume book is an outstanding reference source on allaspects of allergy and allergic diseases. Covering virtually everyallergic condition, from the immunological and molecular basis ofthe allergic response to future trends in allergic diseaseprevention, this new international editorial team (A.B.Kay, JeanBousquet, Pat Holt and Allen Kaplan) have completely revised andupdated the text, from both a scientific and clinical perspective.References will continue to be added to the text until it goes topress making this the most up-to-date book available in thefield. This second edition consists of more than 1,800 pages containedwithin 98 chapters. The price includes a fully searchable companion CD ROM with thecomplete text and over 300 images from the book in full colour. |
| epitope mapping peanut allergy: Allergy and Asthma Massoud Mahmoudi, 2016-06-02 This highly practical, easy-to-read, fully updated and expanded resource offers a wide range of targeted guidelines and insights in allergy medicine. Written by a leading allergy clinician -- along with a renowned group of nationally recognized expert contributors in allergy and immunology, pulmonary, and infectious diseases -- this title is a proven resource for front-line general practitioners, especially primary care physicians. The most clinically relevant information is provided on the pathophysiology, diagnosis, treatment, and prevention of all major allergic disorders. Each chapter has a section on “Evidence-Based Medicine” that introduces one to two recent research publications on the subject, and several chapters have been written by new authors. Covering the entire scope of adult and pediatric allergy and asthma and organized by specific organ which guides the reader to diagnostic and therapeutic solutions quickly and easily, the book offers a wealth of outstanding illustrations, key concepts, management protocols, and updated references. An invaluable contribution to the field, Allergy and Asthma: Practical Diagnosis and Management, 2nd Edition will be of immense value not only to primary care physicians, but also to fellows in training, residents, nurses, nurse practitioners, and medical and allied health students. |
| epitope mapping peanut allergy: Manual of Pediatric Allergy K Nagaraju, 2013-11-30 This manual is a practical guide to paediatric allergy bringing clinicians fully up to date with the most recent advances in the field. Beginning with the pathophysiology of allergy and approach to the patient, the following sections cover the diagnosis of numerous types of allergy including dermatitis, food allergy, ocular allergies and drug allergy. Several chapters are dedicated to asthma. The final sections present the advantages and disadvantages of common drugs used for the management of allergy and asthma, selected lab values in allergy and immunology, and devices for treating allergy and asthma. Key points Practical guide to paediatric allergy for practising paediatricians Covers diagnosis and treatment of many different types of allergy Several chapters dedicated to asthma Includes 230 images, illustrations and tables |
| epitope mapping peanut allergy: Patterson's Allergic Diseases Leslie C. Grammer, Paul A. Greenberger, 2012-02-03 Revised and updated for its Seventh Edition, this highly acclaimed volume is a complete, current, and practical guide to the diagnosis and treatment of allergic disorders. This comprehensive yet concise reference will remain the first choice for residents and practitioners who need guidance to identify an allergy, confirm a diagnosis, or find effective therapies. It will also be an excellent aid for board review. This edition includes discussions of clinical trials in asthma and significant updates on drug allergy, imaging, occupational allergy, and immune deficiency evaluation. A Companion Website will include the fully searchable text and additional illustrations and tables. |
| epitope mapping peanut allergy: Food Allergy Anas M. Abdel Rahman, 2017-09-01 This book explores the recent advances and integrations in molecular technology in food research platforms, which have revolutionized the way we discover and trace potential allergens in our food and drugs and how we utilize that for diagnosis and management. These different technologies for global allergenomic profiling in different kinds of food are discussed, including mass spectrometry, chromatography, and nuclear magnetic resonance. The book also addresses multiomics research with bioinformatics strategies in food allergy in terms of allergen characterization and quantitation, and covers applications in food allergy research from discovery to routine analysis. |
| epitope mapping peanut allergy: Food Allergy, An Issue of Immunology and Allergy Clinics of North America J. Andrew Bird, 2017-11-19 This issue of Immunology and Allergy Clinics, Guest Edited by Dr. J. Andrew Bird, is devoted to Food Allergy. Articles in this outstanding issue include: Prevention of Food Allergies; Epidemiology of Food Allergy; Oral Tolerance Development and Maintenance; Diagnosis of Food Allergy; Food Allergy Management; Interventional Therapies for the Treatment of Food Allergy; Baked Milk and Egg as Oral Immunotherapy; Adjuvant Therapies for Desensitization; Alternative Therapies for Treatment of Food Allergy; Food Protein-Induced Enterocolitis Syndrome (FPIES); Diagnosis and Management of Eosinophilic Esophagitis; and Unfounded Diagnostic Procedures. |
| epitope mapping peanut allergy: Allergy Frontiers:Diagnosis and Health Economics Ruby Pawankar, Stephen T. Holgate, Lanny J. Rosenwasser, 2009-07-10 When I entered the field of allergy in the early 1970s, the standard textbook was a few hundred pages, and the specialty was so compact that texts were often authored entirely by a single individual and were never larger than one volume. Compare this with Allergy Frontiers: Epigenetics, Allergens, and Risk Factors, the present s- volume text with well over 150 contributors from throughout the world. This book captures the explosive growth of our specialty since the single-author textbooks referred to above. The unprecedented format of this work lies in its meticulous attention to detail yet comprehensive scope. For example, great detail is seen in manuscripts dealing with topics such as “Exosomes, naturally occurring minimal antigen presenting units” and “Neuropeptide S receptor 1 (NPSR1), an asthma susceptibility gene.” The scope is exemplified by the unique approach to disease entities normally dealt with in a single chapter in most texts. For example, anaphylaxis, a topic usually confined to one chapter in most textbooks, is given five chapters in Allergy Frontiers. This approach allows the text to employ multiple contributors for a single topic, giving the reader the advantage of being introduced to more than one vi- point regarding a single disease. |
| epitope mapping peanut allergy: Coders' Specialty Guide 2025: Internal Medicine/ Endocrinology/ Wound Care AAPC, 2025-01-31 Hone your reporting accuracy, rev up your productivity, and get prompt and equitable reimbursement with the Coders’ Specialty Guide 2025: Internal Medicine, Endocrinology & Wound Care. All the code details you need are packed into this convenient, specialty-specific resource, along with expert insight on 2025 CPT® and HCPCS Level II procedure code additions and revisions for internal medicine, endocrinology, and wound care. The user-driven layout gives you quick access to essential information for each CPT® code — ICD-10 cross references, RVUs, NCCI edits, Medicare reimbursement info, anatomical illustrations, and definitive coding and billing tips. Plus, a description of the procedure in easy-to-understand terms, so you can confidently translate your providers’ notes into the correct codes. Lighten your workload with helpful features like: Internal medicine, endocrinology, and wound care CPT® and HCPCS Level II procedure and service codes, including 2025 new and revised codes Official descriptors for Category I-III CPT® codes HCPCS Level II codes with lay terms and expert coding and reimbursement advice Practical advice for new and revised 2025 codes Straightforward lay terms explaining how each procedure is performed Medicare fee schedule (hospitals and physicians) RVUs (facility and non-facility) NCCI edits for each specialty procedure Helpful indicators and medical terminology Navigation-friendly headers and tabs Comprehensive code index with page numbers Detailed anatomical illustrations And a lot more! *CPT® is a registered trademark of the American Medical Association. |
| epitope mapping peanut allergy: Pediatric Allergy: Principles and Practice E-Book Stanley J. Szefler, Francisco A Bonilla, Cezmi A Akdis, Hugh Sampson, 2015-05-26 The third edition of Pediatric Allergy continues this title's steadfast tradition of providing comprehensive, authoritative guidance on the day-to-day diagnosis and management of pediatric allergic and immunologic diseases. You'll have the most up-to-date research at hand thanks to an easily accessible full-color format that highlights a host of new chapters, extensive updates, and clinically focused coverage. Whether you're a student, resident, pediatrician or allergist, you'll appreciate this user-friendly and versatile source for providing optimal care! Includes diagnostic tests available for asthma, upper respiratory allergy, and more. Equips you with an understanding of the immune mechanisms underlying allergic diseases. Features coverage of drug allergies and cross-reactivity. Highlights clinical pearls discussing the best approaches to the care and treatment of pediatric patients. Appendices listing common food allergies and autoantibodies in autoimmune diseases make for quick reference to essential material. Expert Consult eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, images, and references from the book on a variety of devices. Revised asthma section examines current asthma guidelines; school-centered asthma programs; exercise-induced asthma; and new directions in asthma therapy. Includes the most current knowledge relating to emerging asthma within young children, medication adherence, and the impact of infection on the natural history of asthma. New information on gene therapy, stem-cell therapy, and a host of new immunodeficiency diseases helps you obtain the best results from the therapeutics for pediatric allergic and immunologic diseases. Features brand-new chapters on immunopathology; diagnostics and management; potential immunotherapeutic strategies for treating food allergies; current status of immunotherapy for food allergy; and biologic therapies. Focused coverage of today's hot topics in pediatric allergy includes the use of targeted biologics to treat specific activation pathways leading to severe allergic diseases; defects of innate immunity; rheumatic diseases of childhood; and inflammatory disorders. Discusses new studies examining potential etiologies for the increase in food allergy and examines potential immunotherapeutic strategies for treating food allergies. New evidence-based principles of medical care help you make the best use of available medications for your patients. |
| epitope mapping peanut allergy: Peptide Microarrays Marina Cretich, Alessandro Gori, 2022-09-24 This detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of considerable value for both advanced users and new-comers in the peptide microarray arena, as the renowned contributors describe full coverage of the aspects related to their workflows, from microchip manufacturing to advanced analytical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peptide Microarrays: Methods and Protocols, Third Edition aims to make the use of peptide microarrays more and more accessible while stimulating further developments to sustain exciting discoveries in the biochemistry and medicine realms. |
| epitope mapping peanut allergy: Allergens and Allergen Immunotherapy Richard F. Lockey, 2004-03-10 Enlarged to describe more than a decade of advances in the immunotherapy of allergic diseases and asthma, this Third Edition contains the most recent studies on the mechanisms, manufacture, and standardization of various allergen groups and their utilization in the treatment of allergic diseases-containing 8 new chapters detailing various pharmacoe |
| epitope mapping peanut allergy: Textbook of Allergy for the Clinician Pudupakkam K. Vedanthan, Harold S. Nelson, Shripad N. Agashe, PA Mahesh, Rohit Katial, 2021-02-04 This is the second and updated version of the Textbook of Allergy for the Clinician. It is a unique book in the field of allergy. The uniqueness lies in the international character of the book with contributors representing both the East and West. This book represents the diversity of issues affecting patients in the specialty of allergy, asthma & immunology. There is some discussion of the basic mechanisms involved and extensive elaboration for the clinicians. This book will appeal to medical students, residents and fellows undergoing training as well as consultants in academic and clinical practice settings. The color plates, especially in the section on Aerobiology, will help in the interaction between the patient and consultant in identifying the plant or flora which is the causative factor. The differences and similarities between the Eastern and Western approaches in the practice of the specialty are being addressed for the first time in a book. |
| epitope mapping peanut allergy: Pediatric Allergy: Principles and Practice E-Book Donald Y. M. Leung, Hugh Sampson, Raif Geha, Stanley J. Szefler, 2010-10-13 Pediatric Allergy supplies the comprehensive guidance you need to diagnose, manage, and treat virtually any type of allergy seen in children. Drs. Leung, Sampson, Geha, and Szefler present the new full-color second edition, with coverage of the diagnosis and management of anaphylaxis, the immune mechanisms underlying allergic disease, the latest diagnostic tests, and more. Treat the full range of pediatric allergic and immunologic diseases through clinically focused coverage relevant to both allergists and pediatricians. Understand the care and treatment of pediatric patients thanks to clinical pearls discussing the best approaches. Easily refer to appendices that list common food allergies and autoantibodies in autoimmune diseases. Apply the newest diagnostic tests available—for asthma, upper respiratory allergy, and more—and know their benefits and contraindications. Treat the allergy at its source rather than the resulting reactions through an understanding of the immune mechanisms underlying allergic diseases. Get coverage of new research that affects methods of patient treatment and discusses potential reasons for increased allergies in some individuals. Better manage potential anaphylaxis cases through analysis of contributing facts and progression of allergic disease. Effectively control asthma and monitor its progression using the new step-by-step approach. Eliminate difficulty in prescribing antibiotics thanks to coverage of drug allergies and cross-reactivity. |
| epitope mapping peanut allergy: Allergens and Allergen Immunotherapy, Second Edition Richard F. Lockey, 1998-09-30 Researchers and clinicians relate their experience with immunotherapy using antigens, which has remained important throughout the enormous advances in immunology over the past 30 years. Among the topics are a historical perspective, outdoor and indoor allergens, venoms, the preparation and administration of extracts, and reactions and other adverse effects. Annotation copyrighted by Book News, Inc., Portland, OR |
| epitope mapping peanut allergy: Epitope Mapping Protocols Ulrich Reineke, Mike Schutkowski, 2009-05-20 Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular BiologyTM series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping. |
| epitope mapping peanut allergy: Food Allergy John M James, A Wesley Burks, Philippe Eigenmann, 2011-09-29 Definitive yet concise, Food Allergy, by Drs. John M. James, Wesley Burks, and Philippe Eigenmann, provides expert guidance for efficient diagnosis and effective management of these increasingly prevalent conditions. The consistent, practical format, with a wealth of case studies, clinical pearls and pitfalls, full-color photos and illustrations, diagrams, and more - along with online access at www.expertconsult.com - make this an ideal quick reference tool for both allergy clinicians and primary care physicians. Quickly reference essential topics thanks to a templated, focused format that includes a wealth of full-color photos and illustrations, diagrams, case studies, and more. Benefit from the knowledge, experience, and global perspective of leading international authors. Deliver the best outcomes by incorporating clinical pearls from experts in the field into your practice. Stay current with timely topics including our latest understanding of non-IgE-mediated food allergies; cross-reactions; future therapies; natural history and prevention; and a review of unproven diagnostic and therapeutic techniques. Access this reference online at ExpertConsult.com, complete with the full text, entire image bank, and more. |
| epitope mapping peanut allergy: Nutrition Support for Infants and Children at Risk Richard J. Cooke, Yvan Vandenplas, Ulrich Wahn, 2007-01-01 Adequate nutrition is especially important during infancy and childhood, where even short periods of malnutrition have long-lasting effects on growth, development and health in adult life. There are several high-risk scenarios for the development of malnutrition, which are the focus of the current publication: Atopic diseases, gastrointestinal disorders, and preterm delivery. For the pediatric allergist it is important to understand the mechanisms regulating IgE responses to food proteins since they may also be the earliest markers for the atopic march. As breastfeeding seems to have only limited effects regarding the atopic march, other measures to modulate infantile immune responses have to be taken, including the use of hypoallergenic formulae or the addition of probiotics or prebiotics to infant formulae. The second part of this book highlights the functional properties of nutrition with regard to diseases of the gastrointestinal tract and the ensuing chronic alterations of gastrointestinal function. Topics addressed include the molecular basis of some diseases, main causes of and nutritional measures in chronic enteropathy, including the role of parenteral and enteral nutrition, stressed mucosa and the role of nutrition in cholestatic liver disease. Nowadays, smaller and more immature infants are surviving - but the smaller the infant, the greater the accrued deficit as nutritional needs change with advancing maturity, and one formulation may not meet all requirements. Furthermore, there are no sensitive, accurate and precise measures of nutritional outcome. The net effect of these uncertainties is that all very-low-birth-weight infants are growth retarded at hospital discharge. Strategies for improving growth in these high-risk infants are at the center of the last part of this publication. |
| epitope mapping peanut allergy: Food Proteins and Peptides Chibuike C Udenigwe, 2021-06-03 This book discusses the chemistry of food proteins and peptides and their relationship with nutritional, functional, and health applications. Bringing together authorities in the field, it provides a comprehensive discussion focused on fundamental chemistries and mechanisms underpinning the structure-function relationships of food proteins and peptides. The functional and bioactive properties hinge on their structural features such as amino acid sequence, molecular size, hydrophobicity, hydrophilicity, and net charges. The book includes coverage of advances in the nutritional and health applications of protein and peptide modifications; novel applications of food proteins and peptides in the development of edible functional biomaterials; advances in the use of proteomics and peptidomics for food proteins and peptide analysis (foodomics); and the relevance of food protein and peptide chemistries in policy and regulation. Research into the fundamental chemistries behind the functional, health and nutritional benefits is burgeoning and has gained the interest of scientists, the industry, regulatory agencies, and consumers. This book fills the knowledge gap providing an excellent source of information for researchers, instructors, students, food and nutrition industry, and policy makers. |
| epitope mapping peanut allergy: Middleton's Allergy E-Book A Wesley Burks, Robyn E. O'Hehir, David H. Broide, Stephen T Holgate, Leonard B. Bacharier, Gurjit K. Khurana Hershey, R. Stokes Peebles, 2019-09-25 Through eight outstanding editions, Middleton's Allergy: Principles and Practice has been the reference of choice for both clinicians and researchers as both a practical reference and an effective self-assessment tool for board preparation. The 9th Edition continues the tradition of excellence with comprehensive coverage of all basic science and clinical applications regarding allergy practice and disease mechanisms. It brings you fully up to date with recent innovations in the diagnosis, prevention, and management of allergic disorders, including emerging global issues, the advent of precision medicine, and new immunologic therapies. - Offers unparalleled depth and up-to-date guidance on the full spectrum of allergy across the lifespan, with significant updates throughout. - Contains new chapters on Innate Lymphoid Cells, Systems Biology, and Treatment of Primary Immunodeficiency Diseases. - Discusses emerging topics such as epidemic thunderstorm asthma and precision medicine in allergic disorders. - Features more than 730 full-color illustrations, including many new cellular and molecular drawings of disease mechanisms. - Includes new Summary of Important Concepts boxes, plus new multiple-choice questions online with explanations and answers. - Features a new team of expert editors and more international contributors for a global perspective of this complex field. - Enhanced eBook version included with purchase. Your enhanced eBook allows you to access all of the text, figures, and references from the book on a variety of devices. |
| epitope mapping peanut allergy: Bioactive Proteins and Peptides as Functional Foods and Nutraceuticals Yoshinori Mine, Eunice Li-Chan, Bo Jiang, 2011-06-09 Bioactive Proteins and Peptides as Functional Foods and Nutraceuticals highlights recent developments of nutraceutical proteins and peptides for the promotion of human health. The book considers fundamental concepts and structure-activity relations for the major classes of nutraceutical proteins and peptides. Coverage includes functional proteins and peptides from numerous sources including: soy, Pacific hake, bovine muscle, peas, wheat, fermented milk, eggs, casein, fish collagen, bovine lactoferrin, and rice. The international panel of experts from industry and academia also reviews current applications and future opportunities within the nutraceutical proteins and peptides sector. |
| epitope mapping peanut allergy: Tree Nuts Cesarettin Alasalvar, Fereidoon Shahidi, 2008-12-17 Nuts have been long perceived as a high-fat, high-calorie food, best avoided or consumed in moderation. However, research is showing that tree nuts are cholesterol-free and contain unsaturated fats which can help lower the risk of heart disease. Nuts also provide essential nutrients such as magnesium, chromium, zinc, and manganese. Like all plant foods they are high in fiber and phytochemicals. This book examines ten popular tree nuts and describes each nut's compositional characteristics, lipid characteristics, effects of consumption on serum lipid profiles, as well as their phytochemicals and role disease prevention. In addition the book covers allergens and uses for non-edible parts. |
| epitope mapping peanut allergy: The Immunoassay Handbook David Wild, 2013-01-21 The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com |
| epitope mapping peanut allergy: Food Toxicology Debasis Bagchi, Anand Swaroop, 2016-11-25 Food toxicology studies how natural or synthetic poisons and toxicants in diverse food products cause harmful, detrimental, or adverse side effects in living organisms. Food toxicology is an important consideration as food supply chain is becoming more multinational in origin, and any contamination or toxic manifestation may cause serious, wide-spread adverse health effects. Food Toxicology covers various aspects of food safety and toxicology, including the study of the nature, properties, effects, and detection of toxic substances in food and their disease manifestations in humans. It will also include other aspects of consumer product safety. The first two chapters discuss the measurement of toxicants and toxicity and the importance of dose-response in food toxicology. Additional chapters discuss the aspects of food associated carcinogenesis and food-derived chemical carcinogenesis, food allergy, pathogens associated with fruits and vegetables, and the detrimental effects of radionuclides exposure. The chapters also cover the most important heavy metal contaminants, namely mercury, lead and vanadium, and Fluoride toxicity, which is extensively discussed in its own chapter. Toxicologists, scientists, researchers in food toxicology, nutritionists, and public health care professionals will find valuable information in this book on all possible intricate areas of food toxicology. |
| epitope mapping peanut allergy: Food Allergens Beatriz Cabanillas, 2023-10-24 This detailed volume provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to allergen structure description, cellular responses, and tolerance induction, through a variety of techniques and animal models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Food Allergens: Methods and Protocols serves as an ideal reference for scientists at all stages involved in the study of food allergy and allergenic components. |
| epitope mapping peanut allergy: Managing Allergens in Food Clare Mills, Harry Wichers, Karin Hoffmann-Sommergruber, 2006-11-30 Controlling allergens in food is a matter of increasing importance for the food industry, especially in light of recent legislation. Effective handling of allergens depends on identifying allergenic ingredients, creating separate production lines for allergen-free products, and effective labelling to inform consumers about which products are safe to eat. Recent attention has also focused on novel methods to manage allergens in food, for example by reducing allergenicity through the prudent selection of raw food materials and improved processing techniques. This important collection provides a clear introduction to food allergens and allergy and offers a comprehensive review of current research contributing to safer food production with regard to allergens.Part one discusses the diagnosis of offending foods and how allergens can affect the quality of life. Expanding on these fundamentals, part two reviews the effect of different methods of food processing on allergens and novel technologies which can reduce the potency of allergens or remove them altogether from foods. The final part of the book covers the key area of risk assessment and allergen management to achieve more uniform standards within the industry. Practical strategies to improve consumer acceptance of GM foods are also discussed.With its distinguished editors and array of international contributors, Managing allergens in food is an essential reference for producers, manufacturers, retailers and all those wishing to improve safety in the food industry. - Presents a cohesive summary of recent research into safer food production - Discusses the effects of food processing on allergens - An international team of editors |
| epitope mapping peanut allergy: Genomics, Proteomics and Metabolomics in Nutraceuticals and Functional Foods Debasis Bagchi, Francis Lau, Manashi Bagchi, 2010-02-04 The nutraceutical and functional food field is rapidly growing in diverse sectors, including academic, commercial and government. This has brought a corresponding shift in research focus and in public awareness. Understanding the relevance of the scientific principles in determining the safety and effectiveness of functional foods and nutraceuticals is increasingly important. It is becoming increasingly evident that genomic research technologies will be used in the coming years and there is a need to provide resources that will facilitate this growth. This book incorporates the most recent advances in the three major sectors of the field within one volume. Genomics, proteomics, and metobolomics represent three major scientific research areas that contribute to nutraceutical and functional food research for studies of effectiveness and safety. |
Accurate and reproducible diagnosis of peanut allergy using …
Identification of immunodominant sequential peanut allergen epitopes enables the diagnosis of peanut allergy with high accuracy. High- throughput peanut allergy diagnostic test validated on …
Accuracy of bead-based epitope assay testing for peanut …
New methods for peanut allergy diagnosis through bead-based epitope assay (BBEA) test-ing with Ara h 2.008 and Ara h 2.019 epitopes have retrospectively demonstrated improved …
Highly Accurate and Reproducible Diagnosis of Peanut Allergy …
Jun 19, 2020 · Here, a novel diagnostic blood-based test using a Bead-Based Epitope Assay (“peanut BBEA”) has been developed on the LEAP cohort and then independently validated …
IgE and IgG epitope mapping by microarray immunoassay …
Microarray technology offers advantages of scale and parallel design over previous methods of epitope mapping. Objective: We designed a redundant peptide microarray for IgE and IgG. …
Epitope-Based IgE Assays and Their Role in Providing …
Jun 30, 2023 · Epitope-based testing detects IgE antibodies that directly bind to antigenic sites of an allergen, providing increased resolution speci ficity and fewer false-positive results for …
Mapping B-cell epitopes of major and minor peanut allergens …
Peanut allergy is an IgE-mediated hypersensitivity reaction char-acterised by activation of mast cells and basophils. Symptoms occur within minutes to a few hours of ingestion, ranging from...
High-resolution epitope mapping by AllerScan reveals …
Chen et al. use a phage-display-based method to simultaneously map IgE and IgG epitopes in peanut-allergic patients undergoing oral immunotherapy (OIT). They identify shared epitopes …
OPEN High-throughput sequencing enhanced phage display …
epitope mapping directly on serum. In this study, we exploit the enormous data output of high-throughput sequencing to investigate samples of great complexity, specifically serum samples …
Direct epitope study using human IgE mAbs against peanut …
Feb 27, 2025 · peanut allergies target highly conserved epitopes on Ara h 2 and/or Ara h 6, or the repetitive DPYSPS motif on Ara h 2. As such, unrelated individuals harbor convergent, …
Specific Peanut Epitopes as a Biomarker for Desensitization …
•Total peanut -IgG4 during EPIT is not highly correlated with treatment response during immunotherapy •Measuring IgE and IgG4 reactivity to epitopes(smaller fragments of allergen …
A bioinformatics approach to identify patients with …
Results—Individuals with peanut allergy showed significantly greater IgE binding and broader epitope diversity than did peanut-tolerant individuals. No significant difference in IgG4 binding …
Serologic measurements for peanut allergy
Allergist-immunologists use serologic peanut allergy testing to maximize test sensitivity and specificity while minimizing cost and inconvenience. Recent advances toward this goal …
Microarray immunoassay: Association of clinical history, in …
Objective: We sought to develop a peptide microarray-based immunoassay to map peanut epitopes by using microliter quantities of serum. Methods: A set of 213 overlapping 20-residue …
AllerGenis and Partners Present Positive Precision Medicine …
Dec 20, 2011 · “The ACAAI presentation describes a highly specific and accurate – with 95% accuracy – precision diagnostic approach to monitor the progress of epicutaneous peanut …
Epitope Mapping The Peanut Panallergen Ara h 8 - The …
microarrays of peptides with peanut allergic and peanut sensitized patient sera for IgE and IgG4 reactivity. METHODS: 15-mer peptides that were offset by 5 amino acids were
Mapping of conformational IgE epitopes of food allergens
Food allergens contain linear and conformational IgE epitopes. Linear epitopes are easy to map by peptide microarrays. Conformational epitopes are challenging as they are created by …
EPOPEX (EPITOPE Open-label Extension), Efficacy and Safety …
epicutaneous immunotherapy in peanut-allergic toddlers: 1-year open-label extension to EPITOPE. Presented at: American College of Allergy, Asthma, and Immunology; November …
AllerGenis Begins Testing Patient Samples for Peanut Allergy …
Allergy Using Proprietary Epitope Mapping Technology HATFIELD, Pa.—June 24, 2020— AllerGenis LLC, a data-driven precision diagnostics company focused on food allergies, today …
Ara h 1-reactive T cells in individuals with peanut allergy
Methods: Tetramer-guided epitope mapping was used to identify the antigenic peptides within the peanut allergen Ara h 1. Subsequently, HLA class II/Ara h 1–specific tetramers were used to …
Linear epitope mapping of peanut allergens demonstrates …
Peanut allergy is considered a major health problem due to its prevalence, persistence, and association with severe symptoms.1 Previous studies suggest that clinical reactivity to …
Accurate and reproducible diagnosis of peanut allergy usi…
Identification of immunodominant sequential peanut allergen epitopes enables the diagnosis of peanut allergy with high accuracy. High- throughput peanut allergy …
Accuracy of bead-based epitope assay testing for peanut allerg…
New methods for peanut allergy diagnosis through bead-based epitope assay (BBEA) test-ing with Ara h 2.008 and Ara h 2.019 epitopes have retrospectively …
Highly Accurate and Reproducible Diagnosis of Peanut Allergy Usi…
Jun 19, 2020 · Here, a novel diagnostic blood-based test using a Bead-Based Epitope Assay (“peanut BBEA”) has been developed on the LEAP cohort and then independently …
IgE and IgG epitope mapping by microarray immunoassay reve…
Microarray technology offers advantages of scale and parallel design over previous methods of epitope mapping. Objective: We designed a redundant peptide microarray …
Epitope-Based IgE Assays and Their Role in Providing Diagnosi…
Jun 30, 2023 · Epitope-based testing detects IgE antibodies that directly bind to antigenic sites of an allergen, providing increased resolution speci ficity and fewer false …
