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| capillary electrophoresis dna analysis: Capillary Gel Electrophoresis Andras Guttman, László Hajba, 2021-12-04 Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses |
| capillary electrophoresis dna analysis: Analysis of Nucleic Acids by Capillary Electrophoresis Christoph Heller, 2013-06-29 Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids. |
| capillary electrophoresis dna analysis: Clinical Applications of Capillary Electrophoresis Stephen M. Palfrey, 2008-02-01 In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today. |
| capillary electrophoresis dna analysis: Advanced Topics in Forensic DNA Typing: Interpretation John M. Butler, 2014-07-28 Advanced Topics in Forensic DNA Typing: Interpretation builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. Intended as a third-edition companion to the Fundamentals of Forensic DNA Typing volume published in 2010 and Advanced Topics in Forensic DNA Typing: Methodology published in 2012, this book contains 16 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field. Over 80 % of the content of this book is new compared to previous editions. - Provides forensic DNA analysts coverage of the crucial topic of DNA mixture interpretation and statistical analysis of DNA evidence - Worked mixture examples illustrate the impact of different statistical approaches for reporting results - Includes allele frequencies for 24 commonly used autosomal STR loci, the revised Quality Assurance Standards which went into effect September 2011 |
| capillary electrophoresis dna analysis: Capillary Electrophoresis and Microchip Capillary Electrophoresis Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho, 2013-02-01 Explores the benefits and limitations of the latest capillary electrophoresis techniques Capillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization. With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample. The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include: Critical evaluation of the use of surfactants in capillary electrophoresis Sampling and quantitative analysis in capillary electrophoresis Capillary electrophoresis with electrochemical detection Overcoming challenges in using microchip electrophoresis for extended monitoring applications Capillary electrophoresis of intact unfractionated heparin and related impurities Microchip capillary electrophoresis for in situ planetary exploration Each chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications. |
| capillary electrophoresis dna analysis: Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques James P. Landers, 2007-12-18 Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields. |
| capillary electrophoresis dna analysis: Forensic DNA Profiling Jo-Anne Bright, Michael Coble, 2019-12-09 DNA testing and its forensic analysis are recognized as the “gold standard” in forensic identification science methods. However, there is a great need for a hands-on step-by-step guide to teach the forensic DNA community how to interpret DNA mixtures, how to assign a likelihood ratio, and how to use the subsequent likelihood ratio when reporting interpretation conclusions. Forensic DNA Profiling: A Practical Guide to Assigning Likelihood Ratios will provide a roadmap for labs all over the world and the next generation of analysts who need this foundational understanding. The techniques used in forensic DNA analysis are based upon the accepted principles of molecular biology. The interpretation of a good-quality DNA profile generated from a crime scene stain from a single-source donor provides an unambiguous result when using the most modern forensic DNA methods. Unfortunately, many crime scene profiles are not single source. They are described as mixed since they contain DNA from two or more individuals. Interpretation of DNA mixtures represents one of the greatest challenges to the forensic DNA analyst. As such, the book introduces terms used to describe DNA profiles and profile interpretation. Chapters explain DNA extraction methods, the polymerase chain reaction (PCR), capillary electrophoresis (CE), likelihood ratios (LRs) and their interpretation, and population genetic models—including Mendelian inheritance and Hardy-Weinberg equilibrium. It is important that analysts understand how LRs are generated in a probabilistic framework, ideally with an appreciation of both semicontinuous and fully continuous probabilistic approaches. KEY FEATURES: • The first book to focus entirely on DNA mixtures and the complexities involved with interpreting the results • Takes a hands-on approach offering theory with worked examples and exercises to be easily understood and implementable by laboratory personnel • New methods, heretofore unpublished previously, provide a means to innovate deconvoluting a mixed DNA profile, assign an LR, and appropriately report the weight of evidence • Includes a chapter on assigning LRs for close relatives (i.e., “It’s not me, it was my brother”), and discusses strategies for the validation of probabilistic genotyping software Forensic DNA Profiling fills the void for labs unfamiliar with LRs, and moving to probabilistic solutions, and for labs already familiar with LRs, but wishing to understand how they are calculated in more detail. The book will be a welcome read for lab professionals and technicians, students, and legal professionals seeking to understand and apply the techniques covered. |
| capillary electrophoresis dna analysis: The FBI DNA Laboratory CreateSpace Independent Publishing Platform, Office of the Investigator General, 2018-07-09 The FBI DNA laboratory : a review of protocol and practice vulnerabilities. |
| capillary electrophoresis dna analysis: Microchip Capillary Electrophoresis Charles Henry, 2006-03-01 Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems. |
| capillary electrophoresis dna analysis: Forensic DNA Analysis Jaiprakash G. Shewale, Ray H. Liu, 2013-08-19 The field of forensic DNA analysis has grown immensely in the past two decades and genotyping of biological samples is now routinely performed in human identification (HID) laboratories. Application areas include paternity testing, forensic casework, family lineage studies, identification of human remains, and DNA databasing. Forensic DNA Analysis: |
| capillary electrophoresis dna analysis: Analytical Techniques in Forensic Science Rosalind Wolstenholme, Sue Jickells, Shari Forbes, 2021-01-26 An in-depth text that explores the interface between analytical chemistry and trace evidence Analytical Techniques in Forensic Science is a comprehensive guide written in accessible terms that examines the interface between analytical chemistry and trace evidence in forensic science. With contributions from noted experts on the topic, the text features a detailed introduction analysis in forensic science and then subsequent chapters explore the laboratory techniques grouped by shared operating principles. For each technique, the authors incorporate specific theory, application to forensic analytics, interpretation, forensic specific developments, and illustrative case studies. Forensic techniques covered include UV-Vis and vibrational spectroscopy, mass spectrometry and gas and liquid chromatography. The applications reviewed include evidence types such as fibers, paint, drugs and explosives. The authors highlight data collection, subsequent analysis, what information has been obtained and what this means in the context of a case. The text shows how analytical chemistry and trace evidence can problem solve the nature of much of forensic analysis. This important text: Puts the focus on trace evidence and analytical science Contains case studies that illustrate theory in practice Includes contributions from experts on the topics of instrumentation, theory, and case examples Explores novel and future applications for analytical techniques Written for undergraduate and graduate students in forensic chemistry and forensic practitioners and researchers, Analytical Techniques in Forensic Science offers a text that bridges the gap between introductory textbooks and professional level literature. |
| capillary electrophoresis dna analysis: Molecular Biomethods Handbook John M. Walker, Ralph Rapley, 2008-11-04 Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs. |
| capillary electrophoresis dna analysis: Handbook of Capillary Electrophoresis, Second Edition James P. Landers, 1996-12-23 Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science. |
| capillary electrophoresis dna analysis: DNA Profiling and DNA Fingerprinting Jörg Epplen, Thomas Lubjuhnn, 1999-05-01 This manual presents practical approaches to using DNA fingerprinting and genetic profiling to answer a variety of biological and medical questions. It provides detailed methodology for setting up and performing experiments and evaluating results. Extensive troubleshooting tips, helpful hints, and advice for daily practice are also included. This will be a useful guide for scientists and researchers engaged in genetic identification and relationship analyses. |
| capillary electrophoresis dna analysis: Analytical Determination of Nicotine and Related Compounds and their Metabolites J.W. Gorrod, P. Jacob III, 1999-11-02 This book provides for the first time a single comprehensive source of information on the analytical chemistry of nicotine and related alkaloids. The editors have brought together scientists from academia and the tobacco industry to describe the state-of-the-art of the chemistry and analytical methods for measurement of nicotine. Both the scope and detail of the book are impressive. Chapters describe the history, pharmacology and toxicology of nicotine, the biosynthesis of nicotine and other alkaloids in the tobacco plant, the general chemistry of nicotine and the analytical methodologies that have been used to measure nicotine and related alkaloids in biological specimens, in tobacco and pharmaceutical products and in tobacco smoke. There is also a comprehensive review of the chemistry and toxicology of nicotine-derived nitrosamines, an important class of tobacco carcinogens. |
| capillary electrophoresis dna analysis: Portable Spectroscopy and Spectrometry, Applications Richard A. Crocombe, Pauline E. Leary, Brooke W. Kammrath, 2021-04-28 The most comprehensive resource available on the many applications of portable spectrometers, including material not found in any other published work Portable Spectroscopy and Spectrometry: Volume Two is an authoritative and up-to-date compendium of the diverse applications for portable spectrometers across numerous disciplines. Whereas Volume One focuses on the specific technologies of the portable spectrometers themselves, Volume Two explores the use of portable instruments in wide range of fields, including pharmaceutical development, clinical research, food analysis, forensic science, geology, astrobiology, cultural heritage and archaeology. Volume Two features contributions by a multidisciplinary team of experts with hands-on experience using portable instruments in their respective areas of expertise. Organized both by instrumentation type and by scientific or technical discipline, 21 detailed chapters cover various applications of portable ion mobility spectrometry (IMS), infrared and near-infrared (NIR) spectroscopy, Raman and x-ray fluorescence (XRF) spectroscopy, smartphone spectroscopy, and many others. Filling a significant gap in literature on the subject, the second volume of Portable Spectroscopy and Spectrometry: Features a significant amount of content published for the first time, or not available in existing literature Brings together work by authors with assorted backgrounds and fields of study Discusses the central role of applications in portable instrument development Covers the algorithms, calibrations, and libraries that are of critical importance to successful applications of portable instruments Includes chapters on portable spectroscopy applications in areas such as the military, agriculture and feed, hazardous materials (HazMat), art conservation, and environmental science Portable Spectroscopy and Spectrometry: Volume Two is an indispensable resource for developers of portable instruments in universities, research institutes, instrument companies, civilian and government purchasers, trainers, operators of portable instruments, and educators and students in portable spectroscopy courses. |
| capillary electrophoresis dna analysis: Medical BioMethods Handbook John M. Walker, Ralph Rapley, 2005-03-18 John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy. |
| capillary electrophoresis dna analysis: Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das, 2021-02-21 The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples. It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples. Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications. |
| capillary electrophoresis dna analysis: Capillary Electrophoresis of Nucleic Acids Keith R. Mitchelson, Jing Cheng, 2008-02-05 The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications. |
| capillary electrophoresis dna analysis: DNA Electrophoresis Protocols for Forensic Genetics Antonio Alonso, 2011-12-08 Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. |
| capillary electrophoresis dna analysis: Forensic DNA Profiling Protocols Patrick J. Lincoln, Jim Thomson, 1998-01-22 This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here -- many from laboratories around the world -- also discuss how to interpret the results in cases of unknown identity and disputed parentage.-- Covers all steps from extraction of human DNA through to analysis and interpretation-- Takes advantage of new methodologies such as capillary electrophoresis-- Clear step-by-step instructions ensure unfailing reproducibility. |
| capillary electrophoresis dna analysis: Forensic DNA Typing: Principles, Applications and Advancements Pankaj Shrivastava, Hirak Ranjan Dash, Jose A. Lorente, Jahangir Imam, 2020-12-11 The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis. Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis. Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science. Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics. The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping. Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level. |
| capillary electrophoresis dna analysis: Pharmaceutical and Biomedical Applications of Capillary Electrophoresis S.M. Lunte, D.M. Radzik, 1996-08-13 The book describes the theory and applications of Capillary Electrophoresis (CE) in the field of pharmaceutical and biomedical analysis. It is targeted towards users who are intimately involved in analytical problems, especially those which involve small samples. This book presents the technique of capillary electrophoresis from the point of view of the serious hands-on use in the field of pharmaceutical and biomedical analysis. An overview of general theory is presented to acquaint the novice with the fundamental principles. A more theoretical approach is taken in the presentation of electrokinetic chromatography. The next chapter discusses advances in column technologies, the preceding chapters having provided a foundation as to how separations occur. In the next three chapters, recognized experts in their fields present fundamentals and state-of-the-art techniques in the areas of optical, electrochemical and mass spectrometric detection. The major focus of the remaining chapters is on applications. This includes the analysis of pharmaceuticals, amino acids and peptides, macromolecules, nucleosides, nucleotides and oligonucleotides. The use of CE for analysis of small ions and separation of biological particles is also discussed. The issue of sample preparation for analysis by CE is addressed, especially as it relates to clinical analysis. |
| capillary electrophoresis dna analysis: Handbook of Smart Materials in Analytical Chemistry Miguel de la Guardia, Francesc A. Esteve-Turrillas, 2019-01-22 A comprehensive guide to smart materials and how they are used in sample preparation, analytical processes, and applications This comprehensive, two-volume handbook provides detailed information on the present state of new materials tailored for selective sample preparation and the legal frame and environmental side effects of the use of smart materials for sample preparation in analytical chemistry, as well as their use in the analytical processes and applications. It covers both methodological and applied analytical aspects, relating to the development and application of new materials for solid-phase extraction (SPE) and solid-phase microextraction (SPME), their use in the different steps and techniques of the analytical process, and their application in specific fields such as water, food, air, pharmaceuticals, clinical sciences and forensics. Every chapter in Handbook of Smart Materials in Analytical Chemistry is written by experts in the field to provide a comprehensive picture of the present state of this key area of analytical sciences and to summarize current applications and research literature in a critical way. Volume 1 covers New Materials for Sample Preparation and Analysis. Volume 2 handles Analytical Processes and Applications. Focuses on the development and applications of smart materials in analytical chemistry Covers both, methodological and applied analytical aspects, for the development of new materials and their use in the different steps and techniques of the analytical process and their application in specific fields Features applications in key areas including water, air, environment, pharma, food, forensic, and clinical Presents the available tools for the use of new materials suitable to aid recognition process to the sample preparation and analysis A key resource for analytical chemists, applied laboratories, and instrument companies Handbook of Smart Materials in Analytical Chemistry, 2V Set is an excellent reference book for specialists and advanced students in the areas of analytical chemistry, including both research and application environments. |
| capillary electrophoresis dna analysis: Capillary Electrophoresis Paul D. Grossman, Joel C. Colburn, 2012-12-02 This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations.This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:Electroosmosis, separation efficiency, and Joule heatingDetection methodsIn-depth discussion of the separation principles and capabilities of the major modes of CESieving gel electrophoresisIsoelectric focusingFree solution CEMicellar electrokinetic capillary chromatographyEntangled polymer matrix-based separationDetailed treatment of the application of CE to a wide range of molecules, supplemented with extensive hands-on illustrations |
| capillary electrophoresis dna analysis: Food Toxicants Analysis Yolanda Picó, 2007-02-07 Food Toxicants Analysis covers different aspects from the field of analytical food toxicology including emerging analytical techniques and applications to detect food allergens, genetically modified organisms, and novel ingredients (including those of functional foods). Focus will be on natural toxins in food plants and animals, cancer modulating substances, microbial toxins in foods (algal, fungal, and bacterial) and all groups of contaminants (i.e., pesticides), persistent organic pollutants, metals, packaging materials, hormones and animal drug residues. The first section describes the current status of the regulatory framework, including the key principles of the EU food law, food safety, and the main mechanisms of enforcement. The second section addresses validation and quality assurance in food toxicants analysis and comprises a general discussion on the use of risk analysis in establishing priorities, the selection and quality control of available analytical techniques. The third section addresses new issues in food toxicant analysis including food allergens and genetically modified organisms (GMOs). The fourth section covers the analysis of organic food toxicants.* step-by-step guide to the use of food analysis techniques* eighteen chapters covering emerging fields in food toxicants analysis* assesses the latest techniques in the field of inorganic analysis |
| capillary electrophoresis dna analysis: Prenatal Diagnosis Brynn Levy, 2018-12-01 This second edition volume expands on the first edition with more detailed methodologies on prenatal testing and diagnosis, and also covers next-generation sequencing techniques. The chapters in this book are divided into three sections: preimplantation genetic testing, traditional prenatal testing, and non-invasive prenatal testing. This book covers topics such as molecular testing for preimplantation genetic diagnosis of single gene disorders; DNA extraction from various types of prenatal specimens; prenatal diagnosis of cystic fibrosis and Tay-Sachs disease; chromosomal SNP microarrays; and isolation of cell-free DNA from maternal plasma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Prenatal Diagnosis, Second Edition is a valuable resource for any researcher interested in reproducing these techniques in their clinical laboratories. |
| capillary electrophoresis dna analysis: Fundamentals of Forensic DNA Typing John M. Butler, 2009-09-30 Fundamentals of Forensic DNA Typing is written with a broad viewpoint. It examines the methods of current forensic DNA typing, focusing on short tandem repeats (STRs). It encompasses current forensic DNA analysis methods, as well as biology, technology and genetic interpretation. This book reviews the methods of forensic DNA testing used in the first two decades since early 1980's, and it offers perspectives on future trends in this field, including new genetic markers and new technologies. Furthermore, it explains the process of DNA testing from collection of samples through DNA extraction, DNA quantitation, DNA amplification, and statistical interpretation. The book also discusses DNA databases, which play an important role in law enforcement investigations. In addition, there is a discussion about ethical concerns in retaining DNA profiles and the issues involved when people use a database to search for close relatives. Students of forensic DNA analysis, forensic scientists, and members of the law enforcement and legal professions who want to know more about STR typing will find this book invaluable. - Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect - Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A.Boxes-- Data, Notes & Applications sections throughout - Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key |
| capillary electrophoresis dna analysis: Epigenomics Krishnarao Appasani, 2012-08-02 Understanding mechanisms of gene regulation that are independent of the DNA sequence itself - epigenetics - has the potential to overthrow long-held views on central topics in biology, such as the biology of disease or the evolution of species. High throughput technologies reveal epigenetic mechanisms at a genome-wide level, giving rise to epigenomics as a new discipline with a distinct set of research questions and methods. Leading experts from academia, the biotechnology and pharmaceutical industries explain the role of epigenomics in a wide range of contexts, covering basic chromatin biology, imprinting at a genome-wide level, and epigenomics in disease biology and epidemiology. Details on assays and sequencing technology serve as an up-to-date overview of the available technological tool kit. A reliable guide for newcomers to the field as well as experienced scientists, this is a unique resource for anyone interested in applying the power of twenty-first-century genomics to epigenetic studies. |
| capillary electrophoresis dna analysis: Forensic DNA Typing John M. Butler, 2005-02-08 Forensic DNA Typing, Second Edition, is the only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome. It examines the science of current forensic DNA typing methods by focusing on the biology, technology, and genetic interpretation of short tandem repeat (STR) markers, which encompass the most common forensic DNA analysis methods used today. The book covers topics from introductory level right up to cutting edge research. High-profile cases are addressed throughout the text, near the sections dealing with the science or issues behind these cases. Ten new chapters have been added to accommodate the explosion of new information since the turn of the century. These additional chapters cover statistical genetic analysis of DNA data, an emerging field of interest to DNA research. Several chapters on statistical analysis of short tandem repeat (STR) typing data have been contributed by Dr. George Carmody, a well-respected professor in forensic genetics. Specific examples make the concepts of population genetics more understandable. This book will be of interest to researchers and practitioners in forensic DNA analysis, forensic scientists, population geneticists, military and private and public forensic laboratories (for identifying individuals through remains), and students of forensic science. *The only book available that specifically covers detailed information on mitochondrial DNA and the Y chromosome*Chapters cover the topic from introductory level right up to cutting edge research*High-profile cases are addressed throughout the book, near the sections dealing with the science or issues behind these cases*NEW TO THIS EDITION: D.N.A. Boxes--boxed Data, Notes & Applications sections throughout the book offer higher levels of detail on specific questions |
| capillary electrophoresis dna analysis: Clinical and Forensic Applications of Capillary Electrophoresis John R. Petersen, Amin A. Mohammad, 2001-04-20 John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology. |
| capillary electrophoresis dna analysis: Microchip Capillary Electrophoresis Protocols Ann Van Schepdael, 2015 Annotation In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis. Chapters focus on small molecule, biomolecule applications, various detection modes, and sample preparation approaches are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microchip Capillary Electrophoresis Protocol aids scientists in continuing to study microchip capillary electrophoresis. |
| capillary electrophoresis dna analysis: Paper Electrophoresis G. E. W. Wolstenholme, Elaine C. P. Millar, 2009-09-14 The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world. |
| capillary electrophoresis dna analysis: Reference Manual on Scientific Evidence , 1994 |
| capillary electrophoresis dna analysis: Capillary Electrophoresis Technology Norberto A. Guzman, 1993-08-10 Tracing the sequence of observations that has led to current understanding in the field, this reference presents the basic concepts, instrumentation and applications of capillary electrophoresis, examining its many features such as high-power resolution, high-mass sensitivity, overall sensitivity and low-sample volume requirements. This work highlights the use of capillary elctrophoresis for the identification, separation, detection and characterization of substances on the molecular counting level. Illustrating the major technical maneuvers for common operations and applications, Capillary Electrophoresis Technology outlines the theoretical concepts and mathematical expressions of capillary electrophoresis; describes advances in instrumentation hardware and detection systems. It explains the advantages and limitations of the different variants of capillary electrophoresis; and provides extra coverage of areas in which capillary electrophoresis has grown increasingly popular, including the identification and characterization of small molecules and macromolecules. Written by experts in the field, this book is aimed at analytical and clinical chemists and biochemists, chemical engineers, biologists, pharmacists, biotechnologists and students in these disciplines. |
| capillary electrophoresis dna analysis: DNA Fingerprinting in Plants Kurt Weising, Hilde Nybom, Markus Pfenninger, Kirsten Wolff, Günter Kahl, 2005-02-28 Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated p |
| capillary electrophoresis dna analysis: Antibody Glycosylation Marija Pezer, 2021-10-22 This book summarizes recent advances in antibody glycosylation research. Covering major topics relevant for immunoglobulin glycosylation - analytical methods, biosynthesis and regulation, modulation of effector functions - it provides new perspectives for research and development in the field of therapeutic antibodies, biomarkers, vaccinations, and immunotherapy. Glycans attached to both variable and constant regions of antibodies are known to affect the antibody conformation, stability, and effector functions. Although it focuses on immunoglobulin G (IgG), the most explored antibody in this context, and unravels the natural phenomena resulting from the mixture of IgG glycovariants present in the human body, the book also discusses other classes of human immunoglobulins, as well as immunoglobulins produced in other species and production systems. Further, it reviews the glycoanalytical methods applied to antibodies and addresses a range of less commonly explored topics, such as automatization and bioinformatics aspects of high-throughput antibody glycosylation analysis. Lastly, the book highlights application areas ranging from the ones already benefitting from antibody glycoengineering (such as monoclonal antibody production), to those still in the research stages (such as exploration of antibody glycosylation as a clinical or biological age biomarker), and the potential use of antibody glycosylation in the optimization of vaccine production and immunization protocols. Summarizing the current knowledge on the broad topic of antibody glycosylation and its therapeutic and biomarker potential, this book will appeal to a wide biomedical readership in academia and industry alike. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. |
| capillary electrophoresis dna analysis: Electrophoresis Budin Michov, 2020-09-07 Electrophoresis is a straightforward but informative analytical method used in biochemistry, biology and medicine. This book combines a detailed discussion of theory and technical application with an elaborate section on troubleshooting and problem solving in electrophoresis. Therefore the book is an important guide for both students and scientists. |
| capillary electrophoresis dna analysis: Mutation Detection R. G. H. Cotton, E. Edkins, S. Forrest, 1998-02-05 Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnbosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field. |
| capillary electrophoresis dna analysis: Electrophoresis in Practice Reiner Westermeier, 2016-05-16 Electrophoresis in Practice ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese. |
Capillary Electrophoresis of DNA - University of Michigan
CE is part of the family of electrophoretic techniques that separate species based upon their size and ionic properties. An ion (i) placed in an electric field will move in the direction parallel to the field towards the oppositely charged electrode with a velocity (vi) defined as follows: See more
DNA Fragment Analysis by Capillary Electrophoresis
DNA Fragment Analysis by Capillary Electrophoresis 7 Contents CHAPTER 5 Data Analysis with GeneMapper® Software and Peak Scanner™ Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . …
Capillary Electrophoresis Fundamentals and Troubleshooting
Analysis of Nucleic Acids by Capillary Electrophoresis (Heller, C., ed.), Vieweg: Germany, Chapter 5, pp. 125-134. Sample is preconcentrated. Effect is inversely proportional to ionic …
Separation of DNA by Capillary Electrophoresis - Springer
Capillary electrophoresis (CE) is an attractive separation technique for DNA analysis due to its high resolving capability, speed, low sample consumption, and its capability to automate …
CAPILLARY ELECTROPHORESIS - kuk.ac.in
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage. CE is performed in a small glass …
A sensitive and robust plasmid analysis method by Capillary
Capillary electrophoresis with Laser Induced Fluorescence detection (CE-LIF) provides a rapid, sensitive, reproducible and automated method for the quantitative analysis of plasmid DNA …
Analysis of double-stranded DNA fragments using capillary …
• Double-stranded DNA (dsDNA) can be analyzed on capillary electrophoresis (CE)-based genetic analyzers with a simple workflow requiring no additional equipment • Sizes of DNA can be …
Capillary Electrophoresis in DNA Analysis - NIST
Why Use CE for DNA Analysis? 1. Injection, separation, and detection are automated. 2. Rapid separations are possible 3. Excellent sensitivity and resolution 4. The time at which any band …
DNA ANALYSIS WITH CAPILLARY ARRAY ELECTROPHORESIS …
Microfabricated capillary array electrophoresis systems have been designed, built and tested that can analyze 12,48 and 96 DNA samples on a single wafer or microplate using individual, 2-fold …
DNA Fragment Analysis by Capillary Electrophoresis - LSU
DNA Fragment Analysis by Capillary Electrophoresis 13 About This Guide IMPORTANT! Before using the products described in this guide, read and understand the information in the Safety …
360643: Introduction to Capillary Electrophoresis - SCIEX
Capillary electrophoresis (CE) is a family of related techniques that employ narrow-bore (20-200 mm i.d.) capillaries to perform high efficiency separa-tions of both large and small molecules.
Capillary Electrophoresis Outline for Workshop in DNA Analysis
Why Use CE for DNA Analysis? 1. Injection, separation, and detection are automated. 2. Rapid separations are possible 3. Excellent sensitivity and resolution 4. The time at which any band …
Principles, Practice, and Evolution of Capillary Electrophoresis …
KEY WORDS: Capillary electrophoresis, capillary-array electrophoresis, forensic DNA analysis, fragment analysis, genotyping, human identification, mitochondrial DNA sequencing, SNP …
Fragment Analysis by capillary electrophoresis
Fragment Analysis by capillary electrophoresis Technique used in genetics to separate and size fluorescently labeled DNA fragments. This fragment analysis 4-step workflow is …
Capillary Electrophoresis in DNA Analysis
• DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification. • Large numbers of copies of specific DNA sequences can be …
Capillary electrophoresis applied to DNA: determining and …
This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone capillary …
Optimization of a Robust and Sensitive Capillary …
Capillary electrophoresis with Laser Induced Fluorescence detection (CE-LIF) provides a rapid, sensitive, reproducible and automated method for the quantitative analysis of plasmid DNA …
DNA fragment analysis by capillary electrophoresis
Analysis of DNA fragments enables a variety of applications, from cell line authentication to detection of aneuploidy. Fragment analysis comprises a series of techniques in which DNA …
Capillary Electrophoresis in DNA Analysis - NIST
As of January 1, 2003, any individual arrested for a violent felony crime (Code of Virginia § 19.2-310.2:1) must provide a buccal sample for DNA analysis, with the resultant profile incorporated …
Capillary Electrophoresis in DNA Analysis - NIST
Butler, J.M., Buel, E., Crivellente, F., McCord, B.R. (2004) Forensic DNA typing by capillary electrophoresis: using the ABI Prism 310 and 3100 Genetic Analyzers for STR analysis. …
Capillary Electrophoresis of DNA - University of Michigan
Capillary electrophoresis (CE), an alternative to conventional slab gel electrophoresis, has developed over the past few years into a very powerful tool for the separation of DNA fragments.
DNA Fragment Analysis by Capillary Electrophoresis
DNA Fragment Analysis by Capillary Electrophoresis 7 Contents CHAPTER 5 Data Analysis with GeneMapper® Software and Peak Scanner™ Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . …
Capillary Electrophoresis Fundamentals and …
Analysis of Nucleic Acids by Capillary Electrophoresis (Heller, C., ed.), Vieweg: Germany, Chapter 5, pp. 125-134. Sample is preconcentrated. Effect is inversely proportional to ionic …
Separation of DNA by Capillary Electrophoresis - Springer
Capillary electrophoresis (CE) is an attractive separation technique for DNA analysis due to its high resolving capability, speed, low sample consumption, and its capability to automate …
CAPILLARY ELECTROPHORESIS - kuk.ac.in
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage. CE is performed in a small glass …
A sensitive and robust plasmid analysis method by Capillary …
Capillary electrophoresis with Laser Induced Fluorescence detection (CE-LIF) provides a rapid, sensitive, reproducible and automated method for the quantitative analysis of plasmid DNA …
Analysis of double-stranded DNA fragments using capillary …
• Double-stranded DNA (dsDNA) can be analyzed on capillary electrophoresis (CE)-based genetic analyzers with a simple workflow requiring no additional equipment • Sizes of DNA can be …
Capillary Electrophoresis in DNA Analysis - NIST
Why Use CE for DNA Analysis? 1. Injection, separation, and detection are automated. 2. Rapid separations are possible 3. Excellent sensitivity and resolution 4. The time at which any band …
DNA ANALYSIS WITH CAPILLARY ARRAY …
Microfabricated capillary array electrophoresis systems have been designed, built and tested that can analyze 12,48 and 96 DNA samples on a single wafer or microplate using individual, 2 …
DNA Fragment Analysis by Capillary Electrophoresis - LSU
DNA Fragment Analysis by Capillary Electrophoresis 13 About This Guide IMPORTANT! Before using the products described in this guide, read and understand the information in the Safety …
360643: Introduction to Capillary Electrophoresis - SCIEX
Capillary electrophoresis (CE) is a family of related techniques that employ narrow-bore (20-200 mm i.d.) capillaries to perform high efficiency separa-tions of both large and small molecules.
Capillary Electrophoresis Outline for Workshop in DNA …
Why Use CE for DNA Analysis? 1. Injection, separation, and detection are automated. 2. Rapid separations are possible 3. Excellent sensitivity and resolution 4. The time at which any band …
Principles, Practice, and Evolution of Capillary …
KEY WORDS: Capillary electrophoresis, capillary-array electrophoresis, forensic DNA analysis, fragment analysis, genotyping, human identification, mitochondrial DNA sequencing, SNP …
Fragment Analysis by capillary electrophoresis
Fragment Analysis by capillary electrophoresis Technique used in genetics to separate and size fluorescently labeled DNA fragments. This fragment analysis 4-step workflow is …
Capillary Electrophoresis in DNA Analysis
• DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification. • Large numbers of copies of specific DNA sequences can be …
Capillary electrophoresis applied to DNA: determining and …
This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone capillary …
Optimization of a Robust and Sensitive Capillary …
Capillary electrophoresis with Laser Induced Fluorescence detection (CE-LIF) provides a rapid, sensitive, reproducible and automated method for the quantitative analysis of plasmid DNA …
DNA fragment analysis by capillary electrophoresis
Analysis of DNA fragments enables a variety of applications, from cell line authentication to detection of aneuploidy. Fragment analysis comprises a series of techniques in which DNA …
Capillary Electrophoresis in DNA Analysis - NIST
As of January 1, 2003, any individual arrested for a violent felony crime (Code of Virginia § 19.2-310.2:1) must provide a buccal sample for DNA analysis, with the resultant profile incorporated …
Capillary Electrophoresis in DNA Analysis - NIST
Butler, J.M., Buel, E., Crivellente, F., McCord, B.R. (2004) Forensic DNA typing by capillary electrophoresis: using the ABI Prism 310 and 3100 Genetic Analyzers for STR analysis. …
