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| diy bacterial gene engineering crispr kit: Zero to Genetic Engineering Hero Justin Pahara, Julie Legault, 2021-08-19 Zero to Genetic Engineering Hero is made to provide you with a first glimpse of the inner-workings of a cell. It further focuses on skill-building for genetic engineering and the Biology-as-a-Technology mindset (BAAT). This book is designed and written for hands-on learners who have little knowledge of biology or genetic engineering. This book focuses on the reader mastering the necessary skills of genetic engineering while learning about cells and how they function. The goal of this book is to take you from no prior biology and genetic engineering knowledge toward a basic understanding of how a cell functions, and how they are engineered, all while building the skills needed to do so. |
| diy bacterial gene engineering crispr kit: CRISPR-Cas Systems Rodolphe Barrangou, John van der Oost, 2012-12-13 CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field. |
| diy bacterial gene engineering crispr kit: Safety of Genetically Engineered Foods National Research Council, Institute of Medicine, Board on Agriculture and Natural Resources, Food and Nutrition Board, Board on Life Sciences, Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health, 2004-07-08 Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps. |
| diy bacterial gene engineering crispr kit: Gmo Sapiens: The Life-changing Science Of Designer Babies Paul Knoepfler, 2015-11-26 This book won the INDIEFAB 2015 Bronze Award for Science (Adult nonfiction).Genetically modified organisms (GMOs) including plants and the foods made from them, are a hot topic of debate today, but soon related technology could go much further and literally change what it means to be human. Scientists are on the verge of being able to create people who are GMOs.Should they do it? Could we become a healthier and 'better' species or might eugenics go viral leading to a real, new world of genetic dystopia? GMO Sapiens tackles such questions by taking a fresh look at the cutting-edge biotech discoveries that have made genetically modified people possible.Bioengineering, genomics, synthetic biology, and stem cells are changing sci-fi into reality before our eyes. This book will capture your imagination with its clear, approachable writing style. It will draw you into the fascinating discussion of the life-changing science of human genetic modification. |
| diy bacterial gene engineering crispr kit: The Code Breaker Walter Isaacson, 2021-03-09 A Best Book of 2021 by Bloomberg BusinessWeek, Time, and The Washington Post The bestselling author of Leonardo da Vinci and Steve Jobs returns with a “compelling” (The Washington Post) account of how Nobel Prize winner Jennifer Doudna and her colleagues launched a revolution that will allow us to cure diseases, fend off viruses, and have healthier babies. When Jennifer Doudna was in sixth grade, she came home one day to find that her dad had left a paperback titled The Double Helix on her bed. She put it aside, thinking it was one of those detective tales she loved. When she read it on a rainy Saturday, she discovered she was right, in a way. As she sped through the pages, she became enthralled by the intense drama behind the competition to discover the code of life. Even though her high school counselor told her girls didn’t become scientists, she decided she would. Driven by a passion to understand how nature works and to turn discoveries into inventions, she would help to make what the book’s author, James Watson, told her was the most important biological advance since his codiscovery of the structure of DNA. She and her collaborators turned a curiosity of nature into an invention that will transform the human race: an easy-to-use tool that can edit DNA. Known as CRISPR, it opened a brave new world of medical miracles and moral questions. The development of CRISPR and the race to create vaccines for coronavirus will hasten our transition to the next great innovation revolution. The past half-century has been a digital age, based on the microchip, computer, and internet. Now we are entering a life-science revolution. Children who study digital coding will be joined by those who study genetic code. Should we use our new evolution-hacking powers to make us less susceptible to viruses? What a wonderful boon that would be! And what about preventing depression? Hmmm…Should we allow parents, if they can afford it, to enhance the height or muscles or IQ of their kids? After helping to discover CRISPR, Doudna became a leader in wrestling with these moral issues and, with her collaborator Emmanuelle Charpentier, won the Nobel Prize in 2020. Her story is an “enthralling detective story” (Oprah Daily) that involves the most profound wonders of nature, from the origins of life to the future of our species. |
| diy bacterial gene engineering crispr kit: CRISPR-Cas Enzymes , 2019-01-25 CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Updated release includes the latest information on the CRISPR-Cas Enzymes |
| diy bacterial gene engineering crispr kit: Preparing for Future Products of Biotechnology National Academies of Sciences, Engineering, and Medicine, Division on Earth and Life Studies, Board on Chemical Sciences and Technology, Board on Agriculture and Natural Resources, Board on Life Sciences, Committee on Future Biotechnology Products and Opportunities to Enhance Capabilities of the Biotechnology Regulatory System, 2017-07-28 Between 1973 and 2016, the ways to manipulate DNA to endow new characteristics in an organism (that is, biotechnology) have advanced, enabling the development of products that were not previously possible. What will the likely future products of biotechnology be over the next 5â€10 years? What scientific capabilities, tools, and/or expertise may be needed by the regulatory agencies to ensure they make efficient and sound evaluations of the likely future products of biotechnology? Preparing for Future Products of Biotechnology analyzes the future landscape of biotechnology products and seeks to inform forthcoming policy making. This report identifies potential new risks and frameworks for risk assessment and areas in which the risks or lack of risks relating to the products of biotechnology are well understood. |
| diy bacterial gene engineering crispr kit: Nature Matilda McQuaid, Hans Gubbels, Gene Bertrand, 2019 An exploration of the ways in which designers are striving to transform our relationship with the natural world. Designers today are striving to transform our relationship with the natural world. While the modern industrial age gave way to designs that vastly improved human enterprise through technology, there were unintended and destructive consequences for the environment. Humans are intrinsically linked to nature yet our actions have frayed this relationship, forcing designers to think more intentionally and to consider the impact of every design decision, from an artifact's manufacture and use to its obsolescence. Designers are aligning with biologists, engineers, agriculturists, environmentalists and many other disciplines to design a more harmonious and regenerative future. Based on these new partnerships, designers are asking different questions and anticipating future challenges, which not only change the design process, but also what design means. Nature: Collaborations in Design includes over sixty-five international projects from the fields of architecture, product design, landscape design, fashion, interactive and communication design, and material research. More than 300 compelling and exquisite photographs, illustrations and content from data visualizations illustrate seven essays, which explain and explore designers' strategies around understanding, simulating, salvaging, facilitating, augmenting, remediating and nurturing nature. Four conversations between scientists and designers delve into topics related to synthetic biology, scientific versus design lexicon, and recent shifts in the meaning of nature with a glossary illuminating scientific, technological and theoretical concepts and processes invoked by the designers. |
| diy bacterial gene engineering crispr kit: Yeast Genetic Engineering Philip J. Barr, Anthony J. Brake, Pablo Valenzuela, 1989 An illustration of the impressive development of yeast gene expression systems accomplished in recent years which demonstrates the power of these systems for the production of proteins in quantities useful as therapeutics, industrial enzymes and research agents. |
| diy bacterial gene engineering crispr kit: The Science and Technology of Growing Young Sergey Young, 2021-08-24 Wall Street Journal, USA Today, and Publishers Weekly bestseller The prospect of living to 200 years old isn’t science fiction anymore. A leader in the emerging field of longevity offers his perspective on what cutting-edge breakthroughs are on the horizon, as well as the practical steps we can take now to live healthily to 100 and beyond. In The Science and Technology of Growing Young, industry investor and insider Sergey Young demystifies the longevity landscape, cutting through the hype and showing readers what they can do now to live better for longer, and offering a look into the exciting possibilities that await us. By viewing aging as a condition that can be cured, we can dramatically revolutionize the field of longevity and make it accessible for everyone. Join Sergey as he gathers insights from world-leading health entrepreneurs, scientists, doctors, and inventors, providing a comprehensive look into the future of longevity in two horizons: • The Near Horizon of Longevity identifies the technological developments that will allow us to live to 150—some of which are already in use—from AI-based diagnostics to gene editing and organ regeneration. • The Far Horizon of Longevity offers a tour of the future of age reversal, and the exciting technologies that will allow us to live healthily to 200, from Internet of Bodies to digital avatars to AI-brain integration. In a bonus chapter, Sergey also showcases 10 longevity choices that we already know and can easily implement to live to 100, distilling the science behind diet, exercise, sleep, mental health, and our environments into attainable habits and lifestyle hacks that anyone can adopt to vastly improve their lives and workplaces. Combining practical advice with an incredible overview of the brave new world to come, The Science and Technology of Growing Young redefines what it means to be human and to grow young. |
| diy bacterial gene engineering crispr kit: The Science and Technology of Growing Young, Updated Edition Sergey Young, 2024-09-24 Wall Street Journal, USA Today, and Publishers Weekly bestseller 2021 Nautilus Book Award Silver Medal Winner - Aging Consciously Category The prospect of living to 200 years old isn’t science fiction anymore. A leader in the emerging field of longevity offers his perspective on what cutting-edge breakthroughs are on the horizon, as well as the practical steps we can take now to live healthily to 100 and beyond. In The Science and Technology of Growing Young, industry investor and insider Sergey Young demystifies the longevity landscape, cutting through the hype and showing readers what they can do now to live better for longer, and offering a look into the exciting possibilities that await us. By viewing aging as a condition that can be cured, we can dramatically revolutionize the field of longevity and make it accessible for everyone. Join Sergey as he gathers insights from world-leading health entrepreneurs, scientists, doctors, and inventors, providing a comprehensive look into the future of longevity in two horizons: The Near Horizon of Longevity identifies the technological developments that will allow us to live to 150—some of which are already in use—from AI-based diagnostics to gene editing and organ regeneration. The Far Horizon of Longevity offers a tour of the future of age reversal, and the exciting technologies that will allow us to live healthily to 200, from Internet of Bodies to digital avatars to AI-brain integration. In a bonus chapter, Sergey also showcases 10 longevity choices that we already know and can easily implement to live to 100, distilling the science behind diet, exercise, sleep, mental health, and our environments into attainable habits and lifestyle hacks that anyone can adopt to vastly improve their lives and workplaces. Combining practical advice with an incredible overview of the brave new world to come, The Science and Technology of Growing Young redefines what it means to be human and to grow young. |
| diy bacterial gene engineering crispr kit: Genome Engineering via CRISPR-Cas9 System Vijai Singh, Pawan K. Dhar, 2020-02-21 Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. |
| diy bacterial gene engineering crispr kit: Change Agent Daniel Suarez, 2017-04-18 New York Times bestselling author Daniel Suarez delivers an exhilarating sci-fi thriller exploring a potential future where CRISPR genetic editing allows the human species to control evolution itself. On a crowded train platform, Interpol agent Kenneth Durand feels the sting of a needle—and his transformation begins... In 2045 Kenneth Durand leads Interpol’s most effective team against genetic crime, hunting down black market labs that perform vanity edits on human embryos for a price. These illegal procedures augment embryos in ways that are rapidly accelerating human evolution—preying on human-trafficking victims to experiment and advance their technology. With the worlds of genetic crime and human trafficking converging, Durand and his fellow Interpol agents discover that one figure looms behind it all: Marcus Demang Wyckes, leader of a powerful and sophisticated cartel known as the Huli jing. But the Huli jing have identified Durand, too. After being forcibly dosed with a radical new change agent, Durand wakes from a coma weeks later to find he’s been genetically transformed into someone else—his most wanted suspect: Wyckes. Now a fugitive, pursued through the genetic underworld by his former colleagues and the police, Durand is determined to restore his original DNA by locating the source of the mysterious—and highly valuable—change agent. But Durand hasn’t anticipated just how difficult locating his enemy will be. With the technology to genetically edit the living, Wyckes and his Huli jing could be anyone and everyone—and they have plans to undermine identity itself. |
| diy bacterial gene engineering crispr kit: Storing Digital Binary Data in Cellular DNA Rocky Termanini, 2020-08-18 Storing Digital Binary Data into Cellular DNA demonstrates how current digital information storage systems have short longevity and limited capacity, also pointing out that their production and consumption of data exceeds supply. Author Rocky Termanini explains the DNA system and how it encodes vast amounts of data, then presents information on the emergence of DNA as a storage technology for the ever-growing stream of data being produced and consumed. The book will be of interest to a range of readers looking to understand this game-changing technology, including researchers in computer science, biomedical engineers, geneticists, physicians, clinicians, law enforcement and cybersecurity experts. - Presents a comprehensive reference on the fascinating and emerging technology of DNA storage - Helps readers understand key concepts on how DNA works as an information storage system - Provides readers with key information on the technologies used to work with DNA data encoding, such as CRISPR - Covers emerging areas of application and ethical concern, such as Smart Cities, cybercrime and cyberwarfare - Includes coverage of synthesizing DNA-encoded data, sequencing DNA-encoded data, and fusing DNA with Digital Immunity Ecosystems (DIE) |
| diy bacterial gene engineering crispr kit: Machine, Platform, Crowd: Harnessing Our Digital Future Andrew McAfee, Erik Brynjolfsson, 2017-06-27 “A clear and crisply written account of machine intelligence, big data and the sharing economy. But McAfee and Brynjolfsson also wisely acknowledge the limitations of their futurology and avoid over-simplification.” —Financial Times In The Second Machine Age, Andrew McAfee and Erik Brynjolfsson predicted some of the far-reaching effects of digital technologies on our lives and businesses. Now they’ve written a guide to help readers make the most of our collective future. Machine | Platform | Crowd outlines the opportunities and challenges inherent in the science fiction technologies that have come to life in recent years, like self-driving cars and 3D printers, online platforms for renting outfits and scheduling workouts, or crowd-sourced medical research and financial instruments. |
| diy bacterial gene engineering crispr kit: Synthetic Sophia Roosth, 2017-03 In the final years of the twentieth century, emigres from mechanical and electrical engineering and computer science resolved that if the aim of biology was to understand life, then making life would yield better theories than experimentation. Sophia Roosth, a cultural anthropologist, takes us into the world of these self-named synthetic biologists who, she shows, advocate not experiment but manufacture, not reduction but construction, not analysis but synthesis. Roosth reveals how synthetic biologists make new living things in order to understand better how life works. What we see through her careful questioning is that the biological features, theories, and limits they fasten upon are determined circularly by their own experimental tactics. This is a story of broad interest, because the active, interested making of the synthetic biologists is endemic to the sciences of our time. |
| diy bacterial gene engineering crispr kit: The CRISPR/Cas System Muhammad Jamal, 2017 The use of CRISPR/Cas technology for genome editing suggests many potential applications, including the alteration of the germline of humans, animals and food crops. The speed and efficiency of the CRISPR/Cas system make it a potentially useful system for gene therapy. In this volume expert international authors provide a useful and timely review of the applications of the CRISPR/Cas system across diverse fields and explore further avenues and research directions of this novel and powerful editing technology. The technology and its application are reviewed with respect to reproduction and development, immunity and genetic diseases, system structure and system specificity. Some of the potential problems of the CRISPR/Cas system are also discussed, in particular the specificity of the system: this remains an important topic as improvement could lead to the more direct and efficient use of the CRISPR/Cas system in clinical settings. The authors also debate ethical concerns associated with this powerful new technology. This volume is a rigorous review of the applications and new opportunities for the CRISPR/Cas system and provides a stimulus for current and future research. An invaluable guide for all scientists working in the fields of genome editing and gene therapy the book is also recommended for all life sciences libraries. |
| diy bacterial gene engineering crispr kit: Science Between Myth and History José G. Perillán, 2021 Science Between Myth and History explores scientific storytelling and its implications on the teaching, practice, and public perception of science. In communicating their science, scientists tend to use historical narratives for important rhetorical purposes. This text explores the implications of doing this. |
| diy bacterial gene engineering crispr kit: Biodefense in the Age of Synthetic Biology National Academies of Sciences, Engineering, and Medicine, Division on Earth and Life Studies, Board on Life Sciences, Board on Chemical Sciences and Technology, Committee on Strategies for Identifying and Addressing Potential Biodefense Vulnerabilities Posed by Synthetic Biology, 2019-01-05 Scientific advances over the past several decades have accelerated the ability to engineer existing organisms and to potentially create novel ones not found in nature. Synthetic biology, which collectively refers to concepts, approaches, and tools that enable the modification or creation of biological organisms, is being pursued overwhelmingly for beneficial purposes ranging from reducing the burden of disease to improving agricultural yields to remediating pollution. Although the contributions synthetic biology can make in these and other areas hold great promise, it is also possible to imagine malicious uses that could threaten U.S. citizens and military personnel. Making informed decisions about how to address such concerns requires a realistic assessment of the capabilities that could be misused. Biodefense in the Age of Synthetic Biology explores and envisions potential misuses of synthetic biology. This report develops a framework to guide an assessment of the security concerns related to advances in synthetic biology, assesses the levels of concern warranted for such advances, and identifies options that could help mitigate those concerns. |
| diy bacterial gene engineering crispr kit: Molecular and Diagnostic Procedures in Mycoplasmology , 1996-02-21 This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. - Diagnostic genetic probes - Immunological tools - Antibiotic sensitivity testing - Diagnosis of specific diseases - Experimental infections - Diagnosis of mycoplasma infections of cell cultures |
| diy bacterial gene engineering crispr kit: A Field Guide to the Apocalypse Athena Aktipis, 2024-04-09 A common sense field guide to understanding, surviving, and thriving in our time of complex chaos and crises. Is this finally it? The end times?Because from COVID-19 to climate catastrophe to the looming AI revolution—not to mention the ever-growing background hum of rage, fear, and anxiety—it’s starting to feel like the party we call civilization is just about over. The good news? It’s always felt that way. Drawing on evolutionary psychology, history, brain science, game theory, and more, cooperation theorist (and, coincidentally, zombie expert) Athena Aktipis reassuringly explains how we, as a species, are hardwired to survive big existential crises. And how we can do so again by leveraging our innate abilities to communicate and cooperate. Pack a ukulele in your prep kit. Practice your risk-management skills. Enlist your crew into a survival team. And embrace the apocalypse. You might just enjoy it. Plus, it will help us build a better and more resilient future for all humankind. |
| diy bacterial gene engineering crispr kit: Defense Against Biological Attacks Sunit K. Singh, Jens H. Kuhn, 2019-03-30 This first volume of a two-volume set describes general aspects, such as the historical view on the topic, the role of information distribution and preparedness of health-care systems and preparedness in emergency cases. Part two describes and discusseses in detail the pathogens and toxins that are potentially used for biological attacks. As such, the book is a valuable resource for faculties engaged in molecular biology, genetic engineering, neurology, biodefense, biosafety & biosecurity, virology, and infectious disease programs, as well as professional medical research organizations. |
| diy bacterial gene engineering crispr kit: Paper-based Diagnostics Kevin J. Land, 2018-12-11 This book explores the status of paper-based diagnostic solutions, or Microfluidics 2.0. The contributors explore: how paper-based tests can be widely distributed and utilized by semi-skilled personnel; how close to commercial applications the technology has become, and what is still required to make paper-based diagnostics the game-changer it can be. The technology is examined through the lens of the World Health Organization’s ASSURED criteria for low-resource countries (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free, and Deliverable to end-users). Its applications have to include: health technology, environmental technology, food safety, and more. This book is appropriate for researchers in these areas, as well as those interested in microfluidics, and includes chapters dedicated to principles such as theory of flow and surface treatments; components such as biomarkers and detection; and current methods of manufacturing. Discusses how paper-based diagnostics can be used in developing countries by comparing current diagnostic tests with the World Health Organization's ASSURED criteria Examines how paper-based diagnostics could be integrated with other technologies, such as printed electronics, and the Internet of Things. Outlines how semi-skilled personnel across a variety of fields can implement paper-based diagnostics |
| diy bacterial gene engineering crispr kit: Decolonizing Science in Latin American Art Joanna Page , 2021-04-15 Projects that bring the ‘hard’ sciences into art are increasingly being exhibited in galleries and museums across the world. In a surge of publications on the subject, few focus on regions beyond Europe and the Anglophone world. Decolonizing Science in Latin American Art assembles a new corpus of art-science projects by Latin American artists, ranging from big-budget collaborations with NASA and MIT to homegrown experiments in artists’ kitchens. While they draw on recent scientific research, these art projects also ‘decolonize’ science. If increasing knowledge of the natural world has often gone hand-in-hand with our objectification and exploitation of it, the artists studied here emphasize the subjectivity and intelligence of other species, staging new forms of collaboration and co-creativity beyond the human. They design technologies that work with organic processes to promote the health of ecosystems, and seek alternatives to the logics of extractivism and monoculture farming that have caused extensive ecological damage in Latin America. They develop do-it-yourself, open-source, commons-based practices for sharing creative and intellectual property. They establish critical dialogues between Western science and indigenous thought, reconnecting a disembedded, abstracted form of knowledge with the cultural, social, spiritual, and ethical spheres of experience from which it has often been excluded. Decolonizing Science in Latin American Art interrogates how artistic practices may communicate, extend, supplement, and challenge scientific ideas. At the same time, it explores broader questions in the field of art, including the relationship between knowledge, care, and curation; nonhuman agency; art and utility; and changing approaches to participation. It also highlights important contributions by Latin American thinkers to themes of global significance, including the Anthropocene, climate change and environmental justice. |
| diy bacterial gene engineering crispr kit: Tripping Points on the Roads to Outwit Terror Yair Sharan, Ted J. Gordon, Elizabeth Florescu, 2021-04-26 This book assesses potential developments of terrorism and ways to prevent it—the growing threats as new technologies become available — and how the same new technologies may help trap those with potential mal-intent. The drumbeat of terror resonates from everywhere; how can we stop it? What are the tripping points along the road and how can we avoid them? Increasingly more people have access to increasingly more information and increasingly more destructive technologies. In the meantime, increasingly advanced technologies help us create an increasingly safer and more harmonious world. Advantages and disadvantages are accelerating each other. While hybrid threats are intensifying, so are the opportunities to address them. But what are the compromises and how can we mitigate them? This book also looks at the unexpected and often random success and failure of policies to counter the evolving terror threat. The various aspects of the terrorism phenomena are presented in a unique way using scenario vignettes, which give the reader a realistic perception of the threat. The combination of positive and negative implications of emerging technologies is describing what might well be one of the most important dimensions of our common future. |
| diy bacterial gene engineering crispr kit: Advances in Synthetic Biology Vijai Singh, 2020-04-13 This book addresses the design of emerging conceptual tools, technologies and systems including novel synthetic parts, devices, circuits, oscillators, biological gates, and small regulatory RNAs (riboregulators and riboswitches), which serve as versatile control elements for regulating gene expression. Synthetic biology, a rapidly growing field that involves the application of engineering principles in biology, is now being used to develop novel systems for a wide range of applications including diagnostics, cell reprogramming, therapeutics, enzymes, vaccines, biomaterials, biofuels, fine chemicals and many more. The book subsequently summarizes recent developments in technologies for assembling synthetic genomes, minimal genomes, synthetic biology toolboxes, CRISPR-Cas systems, cell-free protein synthesis systems and microfluidics. Accordingly, it offers a valuable resource not only for beginners in synthetic biology, but also for researchers, students, scientists, clinicians, stakeholders and policymakers interested in the potential held by synthetic biology. |
| diy bacterial gene engineering crispr kit: Synthetic Biology 2020: Frontiers in Risk Analysis and Governance Benjamin D. Trump, Christopher L. Cummings, Jennifer Kuzma, Igor Linkov, 2019-11-28 Synthetic biology offers powerful remedies for some of the world’s most intractable problems, but these solutions are clouded by uncertainty and risk that few strategies are available to address. The incentives for continued development of this emerging technology are prodigious and obvious, and the public deserves assurances that all potential downsides are duly considered and minimized accordingly. Incorporating social science analysis within the innovation process may impose constraints, but its simultaneous support in making the end products more acceptable to society at large should be considered a worthy trade-off. Contributing authors in this volume represent diverse perspectives related to synthetic biology’s social sciences, and reflect on different areas of risk analysis and governance that have developed for the field. Such perspectives include leading scholarly discussion pertaining to risk assessment, governance, ethics, and communication. The chapters of this volume note that while the first twenty years of synthetic biology development have focused strongly on technological innovation and product development, the next twenty should emphasize the synergy between developers, policymakers, and publics to generate the most beneficial, well governed, and transparent technologies and products possible. Many chapters in this volume provide new data and approaches that demonstrate the feasibility for multi-stakeholder efforts involving policymakers, regulators, industrial developers, workers, experts, and societal representatives to share responsibilities in the production of effective and acceptable governance in the face of uncertain risk probabilities. A full consideration of such perspectives may prevent a world of draconian regulations based on an insufficient or incomplete understanding of the science that underpins synthetic biology, as well as any hesitancy or fear by the public to adopt its eventual products. |
| diy bacterial gene engineering crispr kit: A Crack in Creation Jennifer Doudna, Samuel Sternberg, 2017-06-15 'The most important advance of our era. One of the pioneers of the field describes the exciting hunt for the key breakthrough and what it portends for our future' Walter Isaacson World-famous scientist Jennifer Doudna - winner of the 2020 Nobel Prize in Chemistry for creating the revolutionary gene-editing technique CRISPR - explains her discovery, describes its power to reshape the future of all life and warns of its use. A handful of discoveries have changed the course of human history. This book is about the most recent and potentially the most powerful and dangerous of them all. It is an invention that allows us to rewrite the genetic code that shapes and controls all living beings. As a result, dreams of genetic manipulation have become a stark reality: the power to cure disease and alleviate suffering, as well as to re-design any species, including humans, for our own ends. Jennifer Doudna is the co-inventor of this technology - known as CRISPR - and a scientist of worldwide renown. Writing with fellow researcher Samuel Sternberg, here she provides the definitive account of her discovery, explaining how this wondrous invention works and what it is capable of. She also asks us to consider what our new-found power means: how do we enjoy its unprecedented benefits while avoiding its equally unprecedented dangers? _________________ PRAISE FOR A CRACK IN CREATION: 'The future is in our hands as never before, and this book explains the stakes like no other' George Lucas 'One of the most PIONEERING women in science . . . Exhilarating' Arianna Huffington 'Thrilling' Adam Rutherford 'An instant classic' Siddhartha Mukherjee |
| diy bacterial gene engineering crispr kit: Cell-Free Protein Expression James R. Swartz, 2012-12-06 Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows Cell-Free Translation Systems, edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology. |
| diy bacterial gene engineering crispr kit: Genes, Genomes and Society Röbbe Wünschiers, 2021-12-02 With CRISPR/Cas gene editing tools in hand, we are currently experiencing a new dimension in genetic engineering. But where should the journey lead? Should we treat diseases or better repair them genetically? Will the new genetic engineering, combined with modern reproductive biology, lead to designer babies? And: May we allow a liberalization of these techniques as citizen science? New methods can precisely alter the genetic material - and they leave no traces. This gene and genome surgery thrives on increasing knowledge about the mode of action of genes, those trait-giving regions in the genome. This knowledge is being applied in practice, particularly in the breeding of more resistant and higher-yielding crops. And what about us? The author shows that gene variants have long been associated not only with diseases, but also with nutritional preferences or intelligence. Therapeutic and optimization options are close at hand. What effect does the environment have on the expression of genetic material? Genes can be shaped during a person's lifetime by the environment, nutrition or experiences and thus passed on to their offspring in a modified form. So, does society have a new form of long-term responsibility for (epi)genetic integrity? In this vividly and comprehensibly written book, the author explains the state of genetic engineering without assuming too much prior knowledge and invites an open dialogue on this ambivalent topic. Get your own idea of the fascinating yet intimidating possibilities of genetic engineering. Where do you stand on the issue? With the help of this book, you have the chance to form a differentiated opinion. This book is a translation of the original German 1st edition Generation Gen-Schere by Röbbe Wünschiers, published by Springer Fachmedien Wiesbaden GmbH, part of Springer Nature in 2019. The translation was done with the help of artificial intelligence (machine translation by the service DeepL.com). The text was subsequently revised by the author. Springer Nature works continuously to further the development of tools for the production of books and on the related technologies to support the authors. |
| diy bacterial gene engineering crispr kit: Rice Genomics, Genetics and Breeding Takuji Sasaki, Motoyuki Ashikari, 2018-02-14 This book presents the latest advances in rice genomics, genetics and breeding, with a special focus on their importance for rice biology and how they are breathing new life into traditional genetics. Rice is the main staple food for more than half of the world’s population. Accordingly, sustainable rice production is a crucial issue, particularly in Asia and Africa, where the population continues to grow at an alarming rate. The book’s respective chapters offer new and timely perspectives on the synergistic effects of genomics and genetics in novel rice breeding approaches, which can help address the urgent issue of providing enough food for a global population that is expected to reach 9 billion by 2050. |
| diy bacterial gene engineering crispr kit: How To Build A Dragon Or Die Trying: A Satirical Look At Cutting-edge Science Paul Knoepfler, Julie Knoepfler, 2019-06-25 'This co-authored book explores how advances in cell biology, CRISPR gene editing and bioengineering might be used to make a live dragon. The result is a gloriously tongue-in-check scientific epic … How to Build a Dragon or Die Trying is deliberately flamboyant and outrageous. It’s also funny and smart. Far from a how-to guide for ne’er-do-wells to weaponize reptiles, it is designed to spark healthy curiosity in anyone who enjoys a ripping good science read.'NatureWhat if you could have your own real dragon? While that might seem like just a fantasy, today cutting-edge science has brought us to the point where it might really be possible. This book looks into the possibilities of making living, fire-breathing dragons. The world has been fascinated with dragons for thousands of years. Fictional dragons still have a firm place in pop culture, such as Smaug from The Hobbit as well as the dragons in Game of Thrones and in the How to Train Your Dragon movies. This new book discusses using powerful technologies such as CRISPR gene editing, stem cells, and bioengineering to make real dragons. It also goes through what useful information we can learn from animals such as Pteranodons and amazing present-day creatures in our quest to build actual dragons. The book goes on to discuss the possibility of building other mythical creatures such as unicorns and mermaids. Overall, How to Build A Dragon is also meant as a satirical look at cutting-edge science, and it pokes fun at science hype. Anyone who is interested in dragons or cutting-edge science will enjoy this book! It is written in a humorous, approachable way making science fun and easy to understand, including for young adults.The author is well-known scientist Paul Knoepfler who is familiar to the public for his science, his blog The Niche, and his frequent contributions to lay stories on new science concepts such as stem cells and CRISPR. He also is known for his TED talk on designer babies with more than 1.3 million views, and his two books — . The co-author, his daughter Julie Knoepfler, is a high school student interested in science and writing. She has her own blog on literary and film analysis, and enjoys taking a humorous look at culture through writing. |
| diy bacterial gene engineering crispr kit: Neuro-AIDS Alireza Minagar, Paul Shapshak, 2006 During the past two decades, the world scientific community has witnessed major achievements in our understanding of the pathogenesis of HIV infection of the nervous system and HIV-Associated Dementia (HAD). Despite these giant gains, nervous system involvement during AIDS remains a relentlessly progressive disease with a deadly fate in many cases. This book on NeuroAIDS provides a unique resource for both general neurologists as well as basic neuroscientists with profound interests for research on NeuroAIDS. This book has special emphasis on the mechanisms of disease development and progression of HIV-infected patients with NeuroAIDS. The contributors have provided the readers with comprehensive reviews on clinical manifestations of HAD, mechanisms of HIV entry into the central nervous system, the role of cytokines and chemokines in pathogenesis of NeuroAIDS, drug abuse and NeuroAIDS, virus load in HAD, allostasis in HIV and AIDS, stroke in AIDS patients, and neuroimaging of HIV infection of the central nervous system. In addition, there are chapters on Varicella Zoster virus infection of HIV-seropositive and AIDS patients, as well as the molecular basis for opioids and AIDS virus interactions. |
| diy bacterial gene engineering crispr kit: Making Death Matter Tara Mehrabi, 2016-11-18 This thesis is a contribution to feminist laboratory studies and a critical engagement with the natural sciences, or more precisely research on the biochemical workings and deadly relations of Alzheimer’s disease emanating from a year of field work in a Drosophila fly lab. The natural sciences have been a point of fascination within the field of gender studies for decades. Such sciences produce knowledge on what gets to count as nature and natural, healthy or sick, normal or not, and they have done it with great societal authority and impact throughout European modernity. However, feminist technoscience scholars argue that science and knowledge is socially produced, and political too. Concepts such as nature, animal, human, body, sex, and life itself are not simply given natural realities but phenomena processed through the naturecultures of the laboratory. Situated within such theoretical and methodological approaches, this thesis wonders how scientific facts about Alzheimer’s disease are made in the lab today. What kinds of realities, bodies and ethico-political concerns are enacted? Who gets to live and who gets to die in everyday laboratory practices? Theoretically, the thesis is grounded, particularly, within Karen Barad’s agential realism and posthumanist performativity, and as such it accounts for human and nonhuman entanglements through which AD is performed in the lab in relational ways. In other words, the thesis explores how AD is enacted in the bodies of transgenic fruit flies (Drosophila melanogaster), as these flies embody the disease, live and die with it. Last but not least, the thesis explores the materialities of death, dying, embodiment and biological waste in a biochemistry lab as constitutive parts of the produced knowledge about AD. |
| diy bacterial gene engineering crispr kit: Human Genome Editing National Academies of Sciences, Engineering, and Medicine, National Academy of Medicine, National Academy of Sciences, Committee on Human Gene Editing: Scientific, Medical, and Ethical Considerations, 2017-08-13 Genome editing is a powerful new tool for making precise alterations to an organism's genetic material. Recent scientific advances have made genome editing more efficient, precise, and flexible than ever before. These advances have spurred an explosion of interest from around the globe in the possible ways in which genome editing can improve human health. The speed at which these technologies are being developed and applied has led many policymakers and stakeholders to express concern about whether appropriate systems are in place to govern these technologies and how and when the public should be engaged in these decisions. Human Genome Editing considers important questions about the human application of genome editing including: balancing potential benefits with unintended risks, governing the use of genome editing, incorporating societal values into clinical applications and policy decisions, and respecting the inevitable differences across nations and cultures that will shape how and whether to use these new technologies. This report proposes criteria for heritable germline editing, provides conclusions on the crucial need for public education and engagement, and presents 7 general principles for the governance of human genome editing. |
| diy bacterial gene engineering crispr kit: Chemical Demonstrations Bassam Z. Shakhashiri, 1985 Describes and gives instructions for lecture demonstrations covering acids and bases and liquids, solutions, and colloids |
| diy bacterial gene engineering crispr kit: 최강의 브레인 해킹 엘리자베스 리커, 2023-06-20 디지털 시대, 집중력을 꺼트리는 각종 소음과 방해꾼들이 늘어나며 뇌를 효율적으로 사용하기 더욱 어려워졌다. 하지만 만약 하루 15분만 투자하여 컴퓨터 소프트웨어를 업그레이드하듯이 뇌를 업그레이드할 수 있는 방법이 있다면 어떨까? 우리는 더욱더 많은 지식을 빠르게 익히고, 오랫동안 기억하며, 업무를 효율적으로 처리할 수 있을 것이다. 또한 창의력을 높이고 감정 조절 역시 수월하게 할 수도 있다. 하버드· MIT 출신 신경과학자인 저자는 ‘어떻게 하면 뇌를 업그레이드할 수 있을까?’라는 질문의 해답을 찾고자 10년 동안 수백 개의 연구 논문 및 전문가들과의 인터뷰, 자가 실험 등을 통해 뇌를 변화시키는 효과적인 방법을 기록하고 정리하였다. 이러한 브레인 해킹법을 따라 약물, 운동, 게임, 명상, 식단 등 일상의 사소한 부분을 바꾼다면 누구나 자기 뇌의 ‘진짜’ 주인이 될 수 있다. 이 책에서는 개개인에게 맞춤형으로 최적화한 하루 15분 브레인 해킹 실험과 12주의 샘플 일정표를 제공해 스스로 자신에게 맞는 뇌 활용법을 찾도록 도와준다. ‘테트리스와 명상 중에 무엇이 감정 조절에 효과적일까?’, ‘걷는 것과 자전거 타기 중에 인지 능력을 더 향상시켜주는 것은 무엇일까?’ ‘카페인과 청색광 중에 운전 정확도를 더 높여주는 것은 무엇일까?’와 같이 저자가 제시하는 흥미로운 실험들을 따라 하다 보면 자신도 모르게 저절로 뇌가 업그레이드되는 것을 느낄 수 있을 것이다. 이제 이 책을 따라 뇌를 단련시키며 산만한 뇌에서 벗어나 자신이 원하는 바를 이뤄내보자. |
| diy bacterial gene engineering crispr kit: Global Virology II - HIV and NeuroAIDS Paul Shapshak, Andrew J. Levine, Brian T. Foley, Charurut Somboonwit, Elyse Singer, Francesco Chiappelli, John T. Sinnott, 2017-10-07 This book on NeuroAIDS, a collection of chapters written by experts and specialists from around the world, provides a global perspective on HIV and NeuroAIDS in the field, clinic, and laboratory. The chapters address the comorbidity of HIV and other infectious agents, including Zika virus, Ebola, Chagas disease, TB and HCV. Also discussed are key topics, such as: · Molecular socioepidemiology · Global HIV and NeuroAIDS · Neuropathology · cART and blood-brain barrier penetration · HIV replicative oscillations · HIV and SIV evolution · Psychiatric comorbidities · Neurosyphilis · The examination of current and innovative models of translational research to translational effectiveness |
| diy bacterial gene engineering crispr kit: Beauty Andrea Lipps, Ellen Lupton, 2015 Beauty -- the book, born out of Cooper Hewitt, Smithsonian Design Museum's 2015 Triennial of the same name, curated by Andrea Lipps and Ellen Lupton -- showcases some of the most exciting and provocative design created around the globe during the past three years. These pages aim not to emphasize the hidden beauty in the everyday -- a beloved teapot or favorite shoe -- but to locate transformational beauty in contemporary design that is exuberant, ethereal, atmospheric, experiential, exceptional or sublime. Sixty-two designers represent a vast range of disciplines from architecture, fashion, digital, graphic, and product design, to interiors, hair, nail and lighting design. The objects featured cause us to take pause, catch our breath and get lost in our pursuit to understand or explain them. Designed by the innovative Kimberly Varella, the book is itself a tactile, fluid and provocative interpretation of beauty. Varella's design provides unexpected points of entry, playing with the concepts of beauty by using reflective surfaces, hot pink thread weaving pages together and a heart of the book, from which all else flows. Ethereal, Intricate, Extravagant, Transformative, Transgressive, Elemental and Emergent Beauty are the seven themes. Each section includes the individual designers in conversation with the curators about her or his process and beauty's differing forms, punctuated by rich galleries of their work, generating the ultimate feast for the senses--Publisher's description. |
| diy bacterial gene engineering crispr kit: Heritable Human Genome Editing The Royal Society, National Academy of Sciences, National Academy of Medicine, International Commission on the Clinical Use of Human Germline Genome Editing, 2021-01-16 Heritable human genome editing - making changes to the genetic material of eggs, sperm, or any cells that lead to their development, including the cells of early embryos, and establishing a pregnancy - raises not only scientific and medical considerations but also a host of ethical, moral, and societal issues. Human embryos whose genomes have been edited should not be used to create a pregnancy until it is established that precise genomic changes can be made reliably and without introducing undesired changes - criteria that have not yet been met, says Heritable Human Genome Editing. From an international commission of the U.S. National Academy of Medicine, U.S. National Academy of Sciences, and the U.K.'s Royal Society, the report considers potential benefits, harms, and uncertainties associated with genome editing technologies and defines a translational pathway from rigorous preclinical research to initial clinical uses, should a country decide to permit such uses. The report specifies stringent preclinical and clinical requirements for establishing safety and efficacy, and for undertaking long-term monitoring of outcomes. Extensive national and international dialogue is needed before any country decides whether to permit clinical use of this technology, according to the report, which identifies essential elements of national and international scientific governance and oversight. |
DIY Home Improvement Information | DoItYourself.com
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Apr 16, 2010 · Step 1 - Create Access. Staring down a sealed wall and needing to add blown-in insulation to it can seem like an impossible task, and even more impossible because of the …
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DIY Home Improvement Information | DoItYourself.com
Do it yourself home improvement and diy repair at Doityourself.com. Includes home improvement projects, home repair, kitchen remodeling, plumbing, electrical, painting, real estate, and …
关于自己动手制作（DIY）的网站有哪些推荐？ - 知乎
答主在学校搞社团，就是关于电子DIY，在这里把我常上的一些网站分享一下。有了这些网站，你想做什么都可以啦，当然原子弹就算了。 DIY, 最重要的是开心啦~ 强烈推 …
How to Make Homemade Bee Repellent | DoItYourself.com
Jun 2, 2023 · KC has written how-to articles, guides, and tutorials on different DIY ideas and home improvement projects. KC doesn’t just write about DIY projects, she does …
How to Blow Insulation into Existing Walls | DoItYourself.com
Apr 16, 2010 · Step 1 - Create Access. Staring down a sealed wall and needing to add blown-in insulation to it can seem like an impossible task, and even more impossible because of the …
How To Polish Plastic: 5 DIY Methods | DoItYourself.com
Mar 13, 2023 · KC has written how-to articles, guides, and tutorials on different DIY ideas and home improvement projects. KC doesn’t just write about DIY projects, she does …
DoItYourself.com Community Forums
Help us brainstorm a diy... by Pilot Dane 06-10-25 10:51 AM. 4,641. 24,904. XSleeper. Closets and Home ...
How to Apply Stucco | DoItYourself.com
Sep 14, 2011 · 2. After the bonding agent has been allowed to dry, the stucco can be applied directly to the wall. The application of the stucco should begin with a scratch coat, a 1/4" to …
Weatherproof Wooden Signs: 6 Step DIY Guide | DoItYourself.com
Jul 13, 2023 · A wooden sign is a charming little touch anywhere you put it, immediately creating a look of rustic appeal and DIY energy once it’s been hung up. But it can stop looking pretty …
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How to Seal and Encapsulate a Crawl Space | DoItYourself.com
Pros of DIY Crawl Space Encapsulation Cost. DIY crawl space encapsulation can cost upwards of a couple hundred dollars, but that is a fraction of the cost of having it done professionally …
